Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,488,050 T→C 6.1% intergenic (+15/‑182) ydcF → / → aldA DUF218 superfamily protein, SAM‑binding/aldehyde dehydrogenase A, NAD‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,488,0500TC 6.1% 381.6 / 10.2 129intergenic (+15/‑182)ydcF/aldADUF218 superfamily protein, SAM‑binding/aldehyde dehydrogenase A, NAD‑linked
Reads supporting (aligned to +/- strand):  ref base T (54/67);  new base C (3/5);  total (57/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.30e-01

Only 100 of 133 total aligned reads displayed.

GCGATGGCTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCAC  >  NC_000913/1487902‑1488198
                                                                                                                                                    |                                                                                                                                                    
gCGATGGCTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTtgtg                                                                                                                                                     >  1:118298/1‑150 (MQ=255)
gCGATGGCTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTtgtg                                                                                                                                                     <  1:1370499/150‑1 (MQ=255)
gCGATGGCTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACACTGAAACATGATGCGGTGCTAATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTtgtt                                                                                                                                                     >  1:451848/1‑149 (MQ=255)
  gATGGCTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGaa                                                                                                                                                   <  2:1662414/150‑1 (MQ=255)
  gATGGCTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGaa                                                                                                                                                   <  1:1003726/150‑1 (MQ=255)
   aTGGCTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGacc                                                                                                                                                  >  1:1423013/1‑148 (MQ=255)
   aTGGCTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAAc                                                                                                                                                  >  1:1719058/1‑150 (MQ=255)
   aTGGCTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATACATGCCTGGCAAACGCTGACACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAAc                                                                                                                                                  <  1:870870/150‑1 (MQ=255)
       cTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACt                                                                                                                                              <  2:1382215/150‑1 (MQ=255)
       cTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCGCGTTACGTTAAAAATTGCCCGTTTGTGAACCACt                                                                                                                                              <  2:208884/150‑1 (MQ=255)
       cTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTAGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACt                                                                                                                                              >  2:457375/1‑150 (MQ=255)
       cTACGGTCCACGCGGGCGAGATTTTATAGTTCACTTTGATTTTAAGTAATAAGTCATCCATGCATGGCAAAATCTTAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCTCTCGATAAGTTAAAAATTGCCCGTTTGTGAACCAca                                                                                                                                              >  2:396927/1‑149 (MQ=255)
           ggTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGtt                                                                                                                                          >  1:1217518/1‑150 (MQ=255)
            gTCCCCGCGGTCGAGATTTTATCGTTCACGTTGAGTTTCCGGCAGAAGGCATCCATGAATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGttt                                                                                                                                         <  1:266600/150‑1 (MQ=255)
             tCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTg                                                                                                                                        <  2:602414/150‑1 (MQ=255)
               cccGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCa                                                                                                                                      <  1:168688/150‑1 (MQ=255)
                ccGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCaa                                                                                                                                     <  2:1624497/150‑1 (MQ=255)
                   cgGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACg                                                                                                                                  <  1:1410787/150‑1 (MQ=255)
                     ggCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACggg                                                                                                                                >  1:1452779/1‑150 (MQ=255)
                     ggCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACggg                                                                                                                                <  1:1196359/150‑1 (MQ=255)
                     ggCGAGATGATATGGTTCCCGGTTATTTTCCGGCAGAAGTCTTCCTTTCATTGCAAACGCTGAAACATGATGCGGTGCTCCTCGATTCTATTGAAAGTCTCTCGTTACGGTAAAAATTGCCCGTTTGCAAACAAGTGGTTCACAAACggg                                                                                                                                <  2:286869/150‑1 (MQ=255)
                      gctagaTTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCCTGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGTCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGCAAACAAGTGGTTCACAAACGGGc                                                                                                                               <  1:1045366/147‑1 (MQ=255)
                      gCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCTTCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGCAAACAAGTGGTTCACAAACGGGc                                                                                                                               <  2:1164471/150‑1 (MQ=255)
                      gCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGCAAACAAGTGGTTCACAAACGGGc                                                                                                                               <  2:1044616/150‑1 (MQ=255)
                       cGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCAGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCa                                                                                                                              >  1:612843/1‑150 (MQ=255)
                        gagaTTTTATCGTTCACGTTGATTTTCCGCCAGAAGTCATCCATGCATGGCAACCGCTGAAACATGATGCGGAGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCAt                                                                                                                             <  2:387647/150‑1 (MQ=255)
                         agaTTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATg                                                                                                                            >  2:311433/1‑150 (MQ=255)
                           aTTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGAGGCGGTGCTCATCGAGGCGCTGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGAc                                                                                                                          <  1:327038/150‑1 (MQ=255)
                               tATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGCTGCTCATCGAGGCGAGGGAAAGGCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCt                                                                                                                      <  1:829628/150‑1 (MQ=255)
                                    ttCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACtt                                                                                                                 <  1:157341/150‑1 (MQ=255)
                                      cACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACtttt                                                                                                               >  2:689271/1‑150 (MQ=255)
                                       aCGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTa                                                                                                              >  2:382107/1‑150 (MQ=255)
                                       aCGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTa                                                                                                              <  2:280399/150‑1 (MQ=255)
                                       aCGTTGAGTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAAGATGATGCGGAGCTCATCGAGGCGATTGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTa                                                                                                              <  1:305353/150‑1 (MQ=255)
                                        cGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTAt                                                                                                             >  2:1276631/1‑150 (MQ=255)
                                          ttGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTTCGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTCCAAACGGGCATGACTCCTGACTTTTAttt                                                                                                           >  2:1081346/1‑150 (MQ=255)
                                           tGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGCCTTTTATTTc                                                                                                          >  2:1052522/1‑150 (MQ=255)
                                           tGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTc                                                                                                          <  1:1574436/150‑1 (MQ=255)
                                              ttttCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGTAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGc                                                                                                       <  2:1310416/150‑1 (MQ=255)
                                                   cGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCtttt                                                                                                  >  2:27325/1‑150 (MQ=255)
                                                   cGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCtttt                                                                                                  >  1:1017549/1‑150 (MQ=255)
                                                    ggCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTa                                                                                                 <  2:1183325/150‑1 (MQ=255)
                                                       aGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTc                                                                                              >  2:170720/1‑150 (MQ=255)
                                                        taaGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTcc                                                                                             <  2:1500746/149‑1 (MQ=255)
                                                         aaGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCt                                                                                            <  2:787306/150‑1 (MQ=255)
                                                          aGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCtt                                                                                           <  2:885668/150‑1 (MQ=255)
                                                           gTCATCCATGCATGGCAAACGCTGAAACATGCTGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCttt                                                                                          <  2:1159084/150‑1 (MQ=255)
                                                           gTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCttt                                                                                          >  2:1258183/1‑150 (MQ=255)
                                                           gTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCttt                                                                                          <  2:866806/150‑1 (MQ=255)
                                                            tCATCCATGCATGTCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCtttt                                                                                         <  2:412401/150‑1 (MQ=255)
                                                            tCATCCATGCATGGCAAACGCAGAAACATGATGCGGGGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCtttt                                                                                         <  2:951806/150‑1 (MQ=255)
                                                             cATCCATGCATGGCACAAGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGACCCTGTTGTGTGCAAACGGGGAGTACTCATGACTTTTATTTCAGCATTTTATTCCTGTTa                                                                                        >  2:111084/1‑150 (MQ=255)
                                                             cATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTa                                                                                        >  2:1426397/1‑150 (MQ=255)
                                                              aTCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTac                                                                                       >  1:687354/1‑150 (MQ=255)
                                                                ccATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTacaa                                                                                     >  1:550251/1‑149 (MQ=255)
                                                                  atgcatgGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTAGTGCATTTATTACATTTTACAAtt                                                                                   >  1:33352/1‑150 (MQ=255)
                                                                   tgcatgGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTg                                                                                  <  1:12579/150‑1 (MQ=255)
                                                                        ggCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGttttt                                                                             <  2:247457/150‑1 (MQ=255)
                                                                          cAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTAt                                                                           <  2:1002733/150‑1 (MQ=255)
                                                                          cAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGACATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTAt                                                                           <  2:998712/150‑1 (MQ=255)
                                                                            accGCTGAAACATGATGCGGTGCTCATCTAGGCGATGGAAAGTCCCTCGTTAAGTTAAAAATTGCCCGTTTGTGAACCACTTGGTTGAAAACTGTCATGACTCATTCCATTTATTTCTGGCTTTTATTCATTTTAGACTTGTTTTTAtaa                                                                         >  1:142107/3‑148 (MQ=255)
                                                                                cTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGcc                                                                     >  2:891174/1‑150 (MQ=255)
                                                                                cTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGcc                                                                     <  1:1118678/150‑1 (MQ=255)
                                                                                     aCATGATGCGGTGCTCATCGAGTCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTca                                                                <  2:84268/150‑1 (MQ=255)
                                                                                        tgatgCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAg                                                             <  2:118298/150‑1 (MQ=255)
                                                                                         tattcgttGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCATTTGTTTGCAAACGGGCATGACTCCTGTCTTTTATTTCTGCCTTTTATTCCTTTTTCACTTGTTTTTATGAAGCCATTAACAGa                                                            <  2:677094/143‑1 (MQ=255)
                                                                                            gCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAAtt                                                         <  2:369105/150‑1 (MQ=255)
                                                                                                tGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTcc                                                     <  2:983139/150‑1 (MQ=255)
                                                                                                    cATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGATTTGTCCTTTc                                                 >  1:282198/1‑150 (MQ=255)
                                                                                                     aTCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCa                                                <  2:1206423/150‑1 (MQ=255)
                                                                                                     aTCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCa                                                <  2:1719058/150‑1 (MQ=255)
                                                                                                      tCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCAc                                               <  1:1082914/150‑1 (MQ=255)
                                                                                                       cGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTAAACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACg                                              >  2:1146742/1‑150 (MQ=255)
                                                                                                             gATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCg                                        <  1:546104/150‑1 (MQ=255)
                                                                                                             gATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCg                                        <  1:181138/150‑1 (MQ=255)
                                                                                                              aTGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGt                                       >  2:1483808/1‑150 (MQ=255)
                                                                                                               tGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGtc                                      <  1:664156/150‑1 (MQ=255)
                                                                                                               gggAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGtc                                      <  1:1103041/149‑1 (MQ=255)
                                                                                                                 gAAAGTCTCTCGTTACGTTAAAAATTGCCCGTTTGTGATCCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGtctc                                    <  2:52717/150‑1 (MQ=255)
                                                                                                                 gAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGtctc                                    <  1:526408/150‑1 (MQ=255)
                                                                                                                     gTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCtg                                >  1:1037258/1‑150 (MQ=255)
                                                                                                                      tCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTGGGCAAACGTGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTAGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCtga                               <  2:85831/150‑1 (MQ=255)
                                                                                                                        gCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCtgatg                             >  2:718083/1‑150 (MQ=255)
                                                                                                                        gCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCtgatg                             <  1:1276631/150‑1 (MQ=255)
                                                                                                                        gCTCGTAACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGATGCCATTCAGAGAATTGTCCTTTCACGCTTCCGTCTCTCggatt                             >  1:282335/1‑149 (MQ=255)
                                                                                                                             ttacgttaAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGAtgattga                        >  2:1401851/1‑150 (MQ=255)
                                                                                                                                 gttaAAAATTTCCCGTTTGTTAACAACTTGTTTGCAAACGGGCATGACTCCTGAATTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtt                    <  2:550251/150‑1 (MQ=255)
                                                                                                                                 gttaAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTTTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtt                    >  2:1622487/1‑150 (MQ=255)
                                                                                                                                 gttaAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtt                    >  2:408191/1‑150 (MQ=255)
                                                                                                                                 gttaAAAATTGCCAGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtt                    >  1:738975/1‑150 (MQ=255)
                                                                                                                                  ttaAAAATTGCCCGTTTGTGAACCACTTGTTTACAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtta                   <  2:114912/150‑1 (MQ=255)
                                                                                                                                      gtcatGCCCGTTTGCAAACAAGTGGTTCACAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGttaatta               >  1:778606/5‑150 (MQ=38)
                                                                                                                                      gtcatGCCCGTTTGCAAACAAGTGGTTCACAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGttaatta               >  1:1385532/5‑150 (MQ=38)
                                                                                                                                          tGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGACGATTGATGTTAATTAACaa           <  1:942013/150‑1 (MQ=255)
                                                                                                                                            cccTTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATg         <  2:1128314/150‑1 (MQ=255)
                                                                                                                                            cccGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATg         >  2:709216/1‑150 (MQ=255)
                                                                                                                                            cccGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATg         <  1:1258183/150‑1 (MQ=255)
                                                                                                                                              cGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTGCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGTTTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTa       <  2:1592959/150‑1 (MQ=255)
                                                                                                                                                tttGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTAtt     <  2:1037258/150‑1 (MQ=255)
                                                                                                                                                   gtgAACCACTTGTTTTCAAACGGGAATGACTCATTACTTTTATTTCTGACTTTTATTCCTTTTACACTTGTTTTTATTAAGCCCTTCTCAGAATTGTCCTTTCACGATTCCGTCTCTCTGATTATTGATGTTAATTAACAATGTATTCAc  <  2:935537/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGATGGCTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCAC  >  NC_000913/1487902‑1488198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: