Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,488,057 C→G 7.5% intergenic (+22/‑175) ydcF → / → aldA DUF218 superfamily protein, SAM‑binding/aldehyde dehydrogenase A, NAD‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,488,0570CG 7.5% 389.1 / 15.7 126intergenic (+22/‑175)ydcF/aldADUF218 superfamily protein, SAM‑binding/aldehyde dehydrogenase A, NAD‑linked
Reads supporting (aligned to +/- strand):  ref base C (51/64);  new base G (4/5);  total (55/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 130 total aligned reads displayed.

CTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCACCGAAAACA  >  NC_000913/1487909‑1488206
                                                                                                                                                    |                                                                                                                                                     
cTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACt                                                                                                                                                      <  2:1382215/150‑1 (MQ=255)
cTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCGCGTTACGTTAAAAATTGCCCGTTTGTGAACCACt                                                                                                                                                      <  2:208884/150‑1 (MQ=255)
cTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTAGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACt                                                                                                                                                      >  2:457375/1‑150 (MQ=255)
cTACGGTCCACGCGGGCGAGATTTTATAGTTCACTTTGATTTTAAGTAATAAGTCATCCATGCATGGCAAAATCTTAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCTCTCGATAAGTTAAAAATTGCCCGTTTGTGAACCAca                                                                                                                                                      >  2:396927/1‑149 (MQ=255)
    ggTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGtt                                                                                                                                                  >  1:1217518/1‑150 (MQ=255)
     gTCCCCGCGGTCGAGATTTTATCGTTCACGTTGAGTTTCCGGCAGAAGGCATCCATGAATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGttt                                                                                                                                                 <  1:266600/150‑1 (MQ=255)
      tCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTg                                                                                                                                                <  2:602414/150‑1 (MQ=255)
         ccGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCaa                                                                                                                                             <  2:1624497/150‑1 (MQ=255)
            cgGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACg                                                                                                                                          <  1:1410787/150‑1 (MQ=255)
              ggCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACggg                                                                                                                                        <  1:1196359/150‑1 (MQ=255)
              ggCGAGATGATATGGTTCCCGGTTATTTTCCGGCAGAAGTCTTCCTTTCATTGCAAACGCTGAAACATGATGCGGTGCTCCTCGATTCTATTGAAAGTCTCTCGTTACGGTAAAAATTGCCCGTTTGCAAACAAGTGGTTCACAAACggg                                                                                                                                        <  2:286869/150‑1 (MQ=255)
               gctagaTTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCCTGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGTCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGCAAACAAGTGGTTCACAAACGGGc                                                                                                                                       <  1:1045366/147‑1 (MQ=255)
               gCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCTTCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGCAAACAAGTGGTTCACAAACGGGc                                                                                                                                       <  2:1164471/150‑1 (MQ=255)
               gCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGCAAACAAGTGGTTCACAAACGGGc                                                                                                                                       <  2:1044616/150‑1 (MQ=255)
                cGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCa                                                                                                                                      >  1:866806/1‑150 (MQ=255)
                cGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCAGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCa                                                                                                                                      >  1:612843/1‑150 (MQ=255)
                  agaTTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATg                                                                                                                                    >  2:311433/1‑150 (MQ=255)
                   gaTTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGCAAACAAGTGGTTCACAAACGGGCaatt                                                                                                                                   <  1:1290969/150‑4 (MQ=38)
                        tATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGCTGCTCATCGAGGCGAGGGAAAGGCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCt                                                                                                                              <  1:829628/150‑1 (MQ=255)
                             ttCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACtt                                                                                                                         <  1:157341/150‑1 (MQ=255)
                               cACGTTGATTTTCCGGCAGAAGTCATCCATGCATCGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACtttt                                                                                                                       <  1:478736/150‑1 (MQ=255)
                                aCGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTa                                                                                                                      <  2:280399/150‑1 (MQ=255)
                                aCGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTa                                                                                                                      >  2:382107/1‑150 (MQ=255)
                                aCGTTGAGTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAAGATGATGCGGAGCTCATCGAGGCGATTGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTa                                                                                                                      <  1:305353/150‑1 (MQ=255)
                                 cGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTAt                                                                                                                     >  1:1505852/1‑150 (MQ=255)
                                 cGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTAt                                                                                                                     >  2:829688/1‑150 (MQ=255)
                                   ttGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTTCGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTCCAAACGGGCATGACTCCTGACTTTTAttt                                                                                                                   >  2:1081346/1‑150 (MQ=255)
                                    tGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTc                                                                                                                  <  1:1574436/150‑1 (MQ=255)
                                      aTTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTg                                                                                                                <  2:425731/150‑1 (MQ=255)
                                       ttttCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGTAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGc                                                                                                               <  2:1310416/150‑1 (MQ=255)
                                            cGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCtttt                                                                                                          >  1:1017549/1‑150 (MQ=255)
                                             ggCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTa                                                                                                         <  2:1183325/150‑1 (MQ=255)
                                                aGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTc                                                                                                      >  2:170720/1‑150 (MQ=255)
                                                aGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTc                                                                                                      <  1:311433/150‑1 (MQ=255)
                                                 taaGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTcc                                                                                                     <  2:1500746/149‑1 (MQ=255)
                                                 gAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTcc                                                                                                     <  2:389189/150‑1 (MQ=255)
                                                   aGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCtt                                                                                                   >  2:177105/1‑150 (MQ=255)
                                                   aGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCtt                                                                                                   <  2:885668/150‑1 (MQ=255)
                                                   aGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTAATCCtt                                                                                                   >  2:1144592/1‑150 (MQ=255)
                                                    gTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCttt                                                                                                  >  2:1258183/1‑150 (MQ=255)
                                                    gTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCttt                                                                                                  <  2:866806/150‑1 (MQ=255)
                                                     tCATCCATGCATGGCAAACGCAGAAACATGATGCGGGGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCtttt                                                                                                 <  2:951806/150‑1 (MQ=255)
                                                     gcATCCATGCATGGCAACCGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAATATTGCCCGTTTGTGAACCAATTGTTTGCAAAAGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCtttt                                                                                                 <  2:277217/149‑1 (MQ=255)
                                                      cATCCATGCATGGCACAAGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGACCCTGTTGTGTGCAAACGGGGAGTACTCATGACTTTTATTTCAGCATTTTATTCCTGTTa                                                                                                >  2:111084/1‑150 (MQ=255)
                                                      cATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTa                                                                                                >  2:1426397/1‑150 (MQ=255)
                                                      cATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTAATTCCTTTTa                                                                                                >  1:69469/1‑150 (MQ=255)
                                                       aTCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTac                                                                                               >  1:687354/1‑150 (MQ=255)
                                                         ccATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTacaa                                                                                             >  1:550251/1‑149 (MQ=255)
                                                           atgcatgGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTAGTGCATTTATTACATTTTACAAtt                                                                                           >  1:33352/1‑150 (MQ=255)
                                                           atgcatgGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCATGACTTTTATTTCTGCCTTTTATTCCTTTTACACtt                                                                                           >  1:280521/1‑150 (MQ=255)
                                                            tgcatgGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTg                                                                                          <  1:12579/150‑1 (MQ=255)
                                                            tgcatgGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTg                                                                                          <  1:1710320/150‑1 (MQ=255)
                                                                 ggCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGttttt                                                                                     <  2:247457/150‑1 (MQ=255)
                                                                   cAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTAt                                                                                   >  1:1413594/1‑150 (MQ=255)
                                                                   cAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGACATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTAt                                                                                   <  2:998712/150‑1 (MQ=255)
                                                                     accGCTGAAACATGATGCGGTGCTCATCTAGGCGATGGAAAGTCCCTCGTTAAGTTAAAAATTGCCCGTTTGTGAACCACTTGGTTGAAAACTGTCATGACTCATTCCATTTATTTCTGGCTTTTATTCATTTTAGACTTGTTTTTAtaa                                                                                 >  1:142107/3‑148 (MQ=255)
                                                                         cTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGcc                                                                             <  1:1118678/150‑1 (MQ=255)
                                                                         cTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGcc                                                                             >  2:891174/1‑150 (MQ=255)
                                                                             aaCATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGATACGTTAAAAATTGCCCGTTTGTGAACCACTTTTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTc                                                                         >  2:1161768/1‑150 (MQ=255)
                                                                              aCATGATGCGGTGCTCATCGAGTCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTca                                                                        <  2:84268/150‑1 (MQ=255)
                                                                                 tgatgCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAg                                                                     <  2:118298/150‑1 (MQ=255)
                                                                                  tattcgttGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCATTTGTTTGCAAACGGGCATGACTCCTGTCTTTTATTTCTGCCTTTTATTCCTTTTTCACTTGTTTTTATGAAGCCATTAACAGa                                                                    <  2:677094/143‑1 (MQ=255)
                                                                                     gCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAAtt                                                                 <  2:369105/150‑1 (MQ=255)
                                                                                         tGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTcc                                                             <  2:1017549/150‑1 (MQ=255)
                                                                                         tGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTcc                                                             <  2:983139/150‑1 (MQ=255)
                                                                                              aTCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCa                                                        <  2:1206423/150‑1 (MQ=255)
                                                                                               tCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCAc                                                       <  1:1082914/150‑1 (MQ=255)
                                                                                                cGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACg                                                      >  2:1648203/1‑150 (MQ=255)
                                                                                                cGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTAAACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACg                                                      >  2:1146742/1‑150 (MQ=255)
                                                                                                      gATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCg                                                <  1:181138/150‑1 (MQ=255)
                                                                                                       aTGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGt                                               >  2:1483808/1‑150 (MQ=255)
                                                                                                        gggAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGtc                                              <  1:1103041/149‑1 (MQ=255)
                                                                                                          gAAAGTCTCTCGTTACGTTAAAAATTGCCCGTTTGTGATCCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGtctc                                            <  2:52717/150‑1 (MQ=255)
                                                                                                          gAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGtctc                                            <  1:526408/150‑1 (MQ=255)
                                                                                                              gTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCtg                                        >  1:1037258/1‑150 (MQ=255)
                                                                                                               tCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTGGGCAAACGTGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTAGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCtga                                       <  2:85831/150‑1 (MQ=255)
                                                                                                                 gCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCtgatg                                     >  2:718083/1‑150 (MQ=255)
                                                                                                                 gCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCtgatg                                     <  1:1276631/150‑1 (MQ=255)
                                                                                                                 gCTCGTAACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGATGCCATTCAGAGAATTGTCCTTTCACGCTTCCGTCTCTCggatt                                     >  1:282335/1‑149 (MQ=255)
                                                                                                                      ttacgttaAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGAtgattga                                >  2:1401851/1‑150 (MQ=255)
                                                                                                                          gttaAAAATTTCCCGTTTGTTAACAACTTGTTTGCAAACGGGCATGACTCCTGAATTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtt                            <  2:550251/150‑1 (MQ=255)
                                                                                                                          gttaAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtt                            >  2:408191/1‑150 (MQ=255)
                                                                                                                          gttaAAAATTGCCAGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtt                            >  1:738975/1‑150 (MQ=255)
                                                                                                                           ttaAAAATTGCCCGTTTGTGAACCACTTGTTTACAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGtta                           <  2:114912/150‑1 (MQ=255)
                                                                                                                                 aTTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATTTTAATTAAc                     <  1:605386/150‑1 (MQ=255)
                                                                                                                                   tGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGACGATTGATGTTAATTAACaa                   <  1:942013/150‑1 (MQ=255)
                                                                                                                                     cccGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATg                 >  2:709216/1‑150 (MQ=255)
                                                                                                                                     cccGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATg                 <  1:1258183/150‑1 (MQ=255)
                                                                                                                                     cccGTTTGCAAACAAGTGGTTCACAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATg                 >  2:706138/1‑150 (MQ=255)
                                                                                                                                       cGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTGCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGTTTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTa               <  2:1592959/150‑1 (MQ=255)
                                                                                                                                         tttGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTAtt             <  2:1037258/150‑1 (MQ=255)
                                                                                                                                         tttGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTAtt             <  2:1072297/150‑1 (MQ=255)
                                                                                                                                            gtgAACCACTTGTTTTCAAACGGGAATGACTCATTACTTTTATTTCTGACTTTTATTCCTTTTACACTTGTTTTTATTAAGCCCTTCTCAGAATTGTCCTTTCACGATTCCGTCTCTCTGATTATTGATGTTAATTAACAATGTATTCAc          <  2:935537/150‑1 (MQ=255)
                                                                                                                                              gAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTTATGAACAATGTATTCACCg        >  2:346060/1‑150 (MQ=255)
                                                                                                                                              gAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCACCg        >  1:1149577/1‑150 (MQ=255)
                                                                                                                                               aaCCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAAATGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCACCGa       >  1:626434/1‑150 (MQ=255)
                                                                                                                                                aCCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCACCGaa      <  2:1217518/150‑1 (MQ=255)
                                                                                                                                                    cTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAATCCCTTCACAGAATTGTCCTTTCACTATTCCGTCTCACTGATGATTGATGTTAATTAACAATGTAATAACCGAaaaca  >  2:664153/1‑150 (MQ=255)
                                                                                                                                                    cTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATTTAtt             >  1:102811/1‑139 (MQ=255)
                                                                                                                                                    cTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTAtt             <  2:102811/139‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTACGGTCCCCGCGGGCGAGATTTTATCGTTCACGTTGATTTTCCGGCAGAAGTCATCCATGCATGGCAAACGCTGAAACATGATGCGGTGCTCATCGAGGCGATGGAAAGTCGCTCGTTACGTTAAAAATTGCCCGTTTGTGAACCACTTGTTTGCAAACGGGCATGACTCCTGACTTTTATTTCTGCCTTTTATTCCTTTTACACTTGTTTTTATGAAGCCCTTCACAGAATTGTCCTTTCACGATTCCGTCTCTCTGATGATTGATGTTAATTAACAATGTATTCACCGAAAACA  >  NC_000913/1487909‑1488206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: