Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,499,365 A→T 7.0% Q143L (CAG→CTG)  rimL → ribosomal‑protein‑L7/L12‑serine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,499,3650AT 7.0% 315.7 / 10.9 114Q143L (CAG→CTG) rimLribosomal‑protein‑L7/L12‑serine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (58/47);  new base T (3/5);  total (61/53)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-01

Only 100 of 114 total aligned reads displayed.

GCTGAAATAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGC  >  NC_000913/1499217‑1499512
                                                                                                                                                    |                                                                                                                                                   
gCTGAAATAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAg                                                                                                                                                    >  1:1407178/1‑150 (MQ=255)
 cTGAAATAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAgg                                                                                                                                                   >  1:790454/1‑150 (MQ=255)
  tGAAATAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGt                                                                                                                                                  <  1:999588/150‑1 (MQ=255)
   gAAATAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATAATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTc                                                                                                                                                 >  2:198117/1‑150 (MQ=255)
    aaaTAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCg                                                                                                                                                >  2:1251695/1‑150 (MQ=255)
       tAGGCTACTGGCTGGACGAATCTCAGCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCtt                                                                                                                                             <  1:1281003/150‑1 (MQ=255)
        aGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCttt                                                                                                                                            >  1:878010/1‑150 (MQ=255)
          gCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgc                                                                                                                                          <  1:831707/150‑1 (MQ=255)
           ctcctggctggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTgcg                                                                                                                                         <  2:79520/147‑1 (MQ=255)
            tACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcgg                                                                                                                                        <  1:323833/150‑2 (MQ=255)
            tACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcgg                                                                                                                                        <  2:1392899/150‑2 (MQ=255)
                  ctggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGt                                                                                                                                  >  1:1026238/1‑150 (MQ=255)
                  ctggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGt                                                                                                                                  >  2:1377233/1‑150 (MQ=255)
                   tggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGtt                                                                                                                                 >  2:1205356/1‑150 (MQ=255)
                   tggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGtt                                                                                                                                 <  1:1493147/150‑1 (MQ=255)
                    ggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGttt                                                                                                                                <  1:1595742/150‑1 (MQ=255)
                     gACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGtttt                                                                                                                               >  2:597353/1‑150 (MQ=255)
                     gACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCATGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGtttt                                                                                                                               >  1:667862/1‑150 (MQ=255)
                      aCGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTa                                                                                                                              >  2:705040/1‑150 (MQ=255)
                      aCGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGATTAGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGATTTa                                                                                                                              >  2:1209040/1‑150 (MQ=255)
                       cGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACTCCCAGTATGGTGAACTTAGCCGCTTAGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCTCTATGCTCAAGGGTTTTaa                                                                                                                             >  2:353777/1‑149 (MQ=255)
                            ctcATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTg                                                                                                                        >  1:1038236/1‑150 (MQ=255)
                            ctcATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGAGCAATGGTTTTATCCttt                                                                                                                        >  2:561816/1‑149 (MQ=255)
                               atcaGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAg                                                                                                                     >  2:1234398/1‑150 (MQ=255)
                               atcaGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAg                                                                                                                     >  2:394482/1‑150 (MQ=255)
                               atcaGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAg                                                                                                                     >  2:400724/1‑150 (MQ=255)
                               atcaGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAg                                                                                                                     <  1:1441993/150‑1 (MQ=255)
                                tcaGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAgg                                                                                                                    <  1:1377233/150‑1 (MQ=255)
                                    gggcaggtgATCATTTCTCAGGCGGTGGAGGCATTGATTCATAATTACGCCCCGTCTGGTGAACTTAGACGCTTCGTTATCAAATGTCGGGTGGACAATCCGCAAAGCAACCCGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGc                                                                                                                <  1:1543265/150‑1 (MQ=255)
                                     gggaggggAGCATTTCTCAGGCGCTGGAGGCATTGATTCATCATTACTCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGcc                                                                                                               <  1:44379/147‑1 (MQ=255)
                                     ggcaggtgAACATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGGCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGcc                                                                                                               <  2:189236/150‑1 (MQ=255)
                                     ggcaggggATCATTTCTCAGGCGCTGCCGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGcc                                                                                                               <  2:894388/150‑1 (MQ=255)
                                      gcaggggATCATTTCTCAGGCGCTGCAGTCATTGATTCATCATTAGCCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTGTTATCCTTGAAGGTTGCCt                                                                                                              <  2:92240/150‑1 (MQ=255)
                                       caggggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTATGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTg                                                                                                             <  2:1666660/150‑1 (MQ=255)
                                         ggggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCTAAGCAACCAGGTCGCTTTGCGCAAGGGTATTATACTTGAAGGTTGCCTgca                                                                                                           >  1:1121729/1‑148 (MQ=255)
                                         ggggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGaa                                                                                                           >  1:459470/1‑150 (MQ=255)
                                           ggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGTAGGTTGCCTGAAAc                                                                                                         >  2:1564172/1‑150 (MQ=255)
                                           ggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTGAGCCGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCGTTGCGCAATGGTTTTATCCTTGAAGGTTGCCGGAAAc                                                                                                         >  2:1442633/1‑150 (MQ=255)
                                             aTCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGAGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAg                                                                                                       >  2:85748/1‑150 (MQ=255)
                                              tCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAgg                                                                                                      <  1:1612868/150‑1 (MQ=255)
                                               cATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGc                                                                                                     <  2:470068/150‑1 (MQ=255)
                                                  ttCTCAGGCCCTGCAGGCAAGGCTTCATCCTTACGCCCAGGCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGa                                                                                                  <  2:305358/150‑1 (MQ=255)
                                                    gtcAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGt                                                                                                <  2:1189407/149‑1 (MQ=255)
                                                    ctcAGGCGCTGCAGGCATTGATTCCTCATTACGCCCAGTCTGGTGAACTTAGCCGCTTCGTGATCAAATGTCGGGTGGACACTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGCCTGAGt                                                                                                >  1:243398/1‑150 (MQ=255)
                                                    ctcAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGt                                                                                                >  2:1000275/1‑150 (MQ=255)
                                                     tcAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGtt                                                                                               >  2:728518/1‑150 (MQ=255)
                                                       aGGCGCTGCAGGCTTTGATTCATCATTACGCCGAGTATGGTGTACTTATACGCTTCGTGATCAAATGTCGGGAGGACATTCGGGATAGCAACCAGGTCGGTTTGCGCAATGTTGTTCTCCTTGACGGGTGCCTGAAACAGGCTGAGTTcc                                                                                             <  2:921341/150‑1 (MQ=255)
                                                        ggCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCt                                                                                            >  2:629534/1‑150 (MQ=255)
                                                         gcgcTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTg                                                                                           <  2:834388/150‑1 (MQ=255)
                                                         gcgcTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGGCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTg                                                                                           <  1:1112550/150‑1 (MQ=255)
                                                          cgcTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGa                                                                                          >  2:519538/1‑150 (MQ=255)
                                                           gcTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGaa                                                                                         >  2:1604435/1‑150 (MQ=255)
                                                           gcTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGGCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGaa                                                                                         <  2:691753/150‑1 (MQ=255)
                                                                 ggCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGc                                                                                   <  2:1356781/150‑1 (MQ=255)
                                                                   cATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGAGATCAAATGTCTGGTGGACAATCCGCAAAGCAACCAGGTCGCATTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTCAGTTCCTGAATGATGCCt                                                                                 >  1:524578/1‑150 (MQ=255)
                                                                    aTTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTa                                                                                <  2:1326295/150‑1 (MQ=255)
                                                                       gATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatga                                                                             >  2:1256324/1‑150 (MQ=255)
                                                                          tcatcaTTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgatga                                                                          >  1:1215152/1‑150 (MQ=255)
                                                                             acatTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGTTCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGAtgt                                                                       <  1:1073999/149‑1 (MQ=255)
                                                                              catTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGAtgtg                                                                      >  2:1186600/1‑150 (MQ=255)
                                                                              catTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGACGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGAtgtt                                                                      >  1:814508/1‑149 (MQ=255)
                                                                                    gCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTta                                                                >  1:312555/1‑150 (MQ=255)
                                                                                      ccAGGCTGGTGAACTTAGACGCTTCGTGAGCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata                                                              <  1:645712/150‑1 (MQ=255)
                                                                                       cAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAc                                                             >  1:408102/1‑150 (MQ=255)
                                                                                        aGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCACCCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcg                                                            >  2:1030306/1‑150 (MQ=255)
                                                                                            tGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcgcgcg                                                        <  2:1418819/150‑1 (MQ=255)
                                                                                               tGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtat                                                     <  2:1478550/150‑1 (MQ=255)
                                                                                               tGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtat                                                     >  1:1721405/1‑150 (MQ=255)
                                                                                               tGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtat                                                     >  2:414988/1‑150 (MQ=255)
                                                                                                   cTTAGACGCTTCGTGATCAAATGTCGGGTGGACAAACCGCAAAGCAACCACGTCGCTAAGCGCAATGGTATTATCCTAAAAGGTTGCCTGAAACAGGCTCAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAAGCGCGTATTATc                                                 >  1:359650/1‑150 (MQ=255)
                                                                                                    ttAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGGCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCg                                                <  2:129270/150‑1 (MQ=255)
                                                                                                    attctcGCTTCGTGTAGCAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCg                                                <  1:233373/145‑1 (MQ=255)
                                                                                                        aCGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTc                                            <  2:1407178/150‑1 (MQ=255)
                                                                                                         cGCTTCGTGATCAAATGTCGGGTGGACAATACGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTATATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTTAACTTATACGCGCGTATTATCGATTca                                           >  2:923196/1‑150 (MQ=255)
                                                                                                          gCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTcac                                          <  1:1317807/150‑1 (MQ=255)
                                                                                                           cTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTcaca                                         <  1:400724/150‑1 (MQ=255)
                                                                                                             tCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAAt                                       >  2:1013930/1‑150 (MQ=255)
                                                                                                               gTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGCTTTTATCATTGAAGGTTGCCTGAAACAGGTTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATaa                                     >  1:497555/1‑150 (MQ=255)
                                                                                                                     aaaTGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGc                               >  1:106674/1‑150 (MQ=255)
                                                                                                                     aaaTGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGc                               >  2:908352/1‑150 (MQ=255)
                                                                                                                      aaTGTGGGGGGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGATACAGGCTGAGTTCCTGAATGTTGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGcc                              <  2:524578/150‑1 (MQ=255)
                                                                                                                      aaTGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGcc                              <  1:519538/150‑1 (MQ=255)
                                                                                                                       aTGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCa                             <  1:993018/150‑1 (MQ=255)
                                                                                                                        tGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAg                            <  1:1714630/150‑1 (MQ=255)
                                                                                                                        tGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAg                            <  1:1123468/150‑1 (MQ=255)
                                                                                                                            gggTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAgcggc                        >  1:608633/1‑150 (MQ=255)
                                                                                                                              gTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGt                      <  2:459470/150‑1 (MQ=255)
                                                                                                                                ggACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCATTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATTATGTGAACTTATACGCGCGTATTATCGAGTCACAATAACCCTGCCAGCGGCGtta                    >  2:1285104/1‑149 (MQ=255)
                                                                                                                                 gACAATCCGCAAAGCAACCAGGTCGCTTTGCGCATTGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTcg                   >  2:832043/1‑150 (MQ=255)
                                                                                                                                  ccattgcGCAAAGCGACCTGGTTGCTTTGCGGAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTcgc                  >  1:1129329/7‑150 (MQ=255)
                                                                                                                                   cattgcGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTcgcg                 >  2:760353/6‑150 (MQ=255)
                                                                                                                                    aaTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGt                >  2:1520397/1‑150 (MQ=255)
                                                                                                                                        cGCAAAGCAACCAGGTCGCGTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATg            <  1:847723/150‑1 (MQ=255)
                                                                                                                                          cAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATgcg          >  1:974736/1‑150 (MQ=255)
                                                                                                                                            aaGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGct        >  1:367837/1‑150 (MQ=255)
                                                                                                                                            aaGCAACCAGGTAGTTTTGCGCTATGGTTTTTTCCTTGAAGGTTGCATGAAACTGTCTGAGTGCCTGAATTTTGCCTATGATGATGGGAACTTATACGCGCGTATTATCGATTCACAATAACGATGTCAGCGGCGTTCTCGTAATGCGct        <  2:821527/150‑1 (MQ=255)
                                                                                                                                              gCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGctcc      <  2:1551943/150‑1 (MQ=255)
                                                                                                                                              gCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGctcc      <  1:1565743/150‑1 (MQ=255)
                                                                                                                                                 aCCAGGTCGCTTTGCGCAATGGTTTTATCCTTGCAGGTGGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCg   <  1:1442633/150‑1 (MQ=255)
                                                                                                                                                  ccACGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGTCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGACAGCGGCGTTCGCGTAATGAGCTCCTCGc  <  1:353777/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCTGAAATAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGC  >  NC_000913/1499217‑1499512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: