Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,499,369 C→T 7.3% V144V (GTC→GTT rimL → ribosomal‑protein‑L7/L12‑serine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,499,3690CT 7.3% 341.6 / 10.3 110V144V (GTC→GTTrimLribosomal‑protein‑L7/L12‑serine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (55/46);  new base T (3/5);  total (58/52)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

Only 100 of 111 total aligned reads displayed.

GAAATAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGC  >  NC_000913/1499220‑1499512
                                                                                                                                                     |                                                                                                                                               
 aaaTAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCg                                                                                                                                                >  2:1251695/1‑150 (MQ=255)
     aGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCttt                                                                                                                                            >  1:878010/1‑150 (MQ=255)
       gCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgc                                                                                                                                          <  1:831707/150‑1 (MQ=255)
        ctcctggctggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTgcg                                                                                                                                         <  2:79520/147‑1 (MQ=255)
         tACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcgg                                                                                                                                        <  2:1392899/150‑2 (MQ=255)
         tACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcgg                                                                                                                                        <  1:323833/150‑2 (MQ=255)
           ctggctggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGATGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGATTGCTTTgcggat                                                                                                                                      <  2:832778/150‑4 (MQ=255)
             ggctggACGAATCTCATCAGGGGCAGGGGAGCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCTGGTGGACAAACCGCAAAGCAACCAGGTCGCTTTGCGCAATg                                                                                                                                    >  1:220472/1‑150 (MQ=255)
               ctggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAACTGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcggattgtc                                                                                                                                  <  1:1141157/150‑8 (MQ=255)
               ctggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGt                                                                                                                                  >  1:1026238/1‑150 (MQ=255)
               ctggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGt                                                                                                                                  >  2:1377233/1‑150 (MQ=255)
                tggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGtt                                                                                                                                 >  2:1205356/1‑150 (MQ=255)
                tggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGtt                                                                                                                                 <  1:1493147/150‑1 (MQ=255)
                 ggACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGttt                                                                                                                                <  1:1595742/150‑1 (MQ=255)
                  gACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGtttt                                                                                                                               >  2:597353/1‑150 (MQ=255)
                  gACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCATGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGtttt                                                                                                                               >  1:667862/1‑150 (MQ=255)
                   aCGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTa                                                                                                                              >  2:705040/1‑150 (MQ=255)
                   aCGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGATTAGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGATTTa                                                                                                                              >  2:1209040/1‑150 (MQ=255)
                    cGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACTCCCAGTATGGTGAACTTAGCCGCTTAGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCTCTATGCTCAAGGGTTTTaa                                                                                                                             >  2:353777/1‑149 (MQ=255)
                      aaTCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATcc                                                                                                                           <  2:1286369/150‑1 (MQ=255)
                         ctcATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTg                                                                                                                        >  1:1038236/1‑150 (MQ=255)
                         ctcATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGAGCAATGGTTTTATCCttt                                                                                                                        >  2:561816/1‑149 (MQ=255)
                            atcaGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAg                                                                                                                     <  1:1441993/150‑1 (MQ=255)
                            atcaGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAg                                                                                                                     >  2:394482/1‑150 (MQ=255)
                            atcaGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAg                                                                                                                     >  2:400724/1‑150 (MQ=255)
                            atcaGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAg                                                                                                                     >  2:1234398/1‑150 (MQ=255)
                             tcaGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAgg                                                                                                                    <  1:1377233/150‑1 (MQ=255)
                                ggggcaggggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTg                                                                                                                 <  1:533149/150‑1 (MQ=255)
                                 gggcaggtgATCATTTCTCAGGCGGTGGAGGCATTGATTCATAATTACGCCCCGTCTGGTGAACTTAGACGCTTCGTTATCAAATGTCGGGTGGACAATCCGCAAAGCAACCCGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGc                                                                                                                <  1:1543265/150‑1 (MQ=255)
                                  gggaggggAGCATTTCTCAGGCGCTGGAGGCATTGATTCATCATTACTCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGcc                                                                                                               <  1:44379/147‑1 (MQ=255)
                                  ggcaggtgAACATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGGCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGcc                                                                                                               <  2:189236/150‑1 (MQ=255)
                                  ggcaggggATCATTTCTCAGGCGCTGCCGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGcc                                                                                                               <  2:894388/150‑1 (MQ=255)
                                   gcaggggATCATTTCTCAGGCGCTGCAGTCATTGATTCATCATTAGCCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTGTTATCCTTGAAGGTTGCCt                                                                                                              <  2:92240/150‑1 (MQ=255)
                                    caggggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTATGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTg                                                                                                             <  2:1666660/150‑1 (MQ=255)
                                      ggggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCTAAGCAACCAGGTCGCTTTGCGCAAGGGTATTATACTTGAAGGTTGCCTgca                                                                                                           >  1:1121729/1‑148 (MQ=255)
                                      ggggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGaa                                                                                                           >  1:459470/1‑150 (MQ=255)
                                        ggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGTAGGTTGCCTGAAAc                                                                                                         >  2:1564172/1‑150 (MQ=255)
                                        ggATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTGAGCCGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCGTTGCGCAATGGTTTTATCCTTGAAGGTTGCCGGAAAc                                                                                                         >  2:1442633/1‑150 (MQ=255)
                                          aTCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGAGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAg                                                                                                       >  2:85748/1‑150 (MQ=255)
                                            cATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGc                                                                                                     <  2:470068/150‑1 (MQ=255)
                                              tttCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTg                                                                                                   >  2:1092031/1‑150 (MQ=255)
                                               ttCTCAGGCCCTGCAGGCAAGGCTTCATCCTTACGCCCAGGCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGa                                                                                                  <  2:305358/150‑1 (MQ=255)
                                                 gtcAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGt                                                                                                <  2:1189407/149‑1 (MQ=255)
                                                 ctcAGGCGCTGCAGGCATTGATTCCTCATTACGCCCAGTCTGGTGAACTTAGCCGCTTCGTGATCAAATGTCGGGTGGACACTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGCCTGAGt                                                                                                >  1:243398/1‑150 (MQ=255)
                                                  tcAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGtt                                                                                               >  2:728518/1‑150 (MQ=255)
                                                    aGGCGCTGCAGGCTTTGATTCATCATTACGCCGAGTATGGTGTACTTATACGCTTCGTGATCAAATGTCGGGAGGACATTCGGGATAGCAACCAGGTCGGTTTGCGCAATGTTGTTCTCCTTGACGGGTGCCTGAAACAGGCTGAGTTcc                                                                                             <  2:921341/150‑1 (MQ=255)
                                                     ggCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCt                                                                                            >  2:629534/1‑150 (MQ=255)
                                                      gcgcTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTg                                                                                           <  2:834388/150‑1 (MQ=255)
                                                      gcgcTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGGCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTg                                                                                           <  1:1112550/150‑1 (MQ=255)
                                                       cgcTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGa                                                                                          >  2:519538/1‑150 (MQ=255)
                                                        gcTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGaa                                                                                         >  2:1604435/1‑150 (MQ=255)
                                                        gcTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGGCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGaa                                                                                         <  2:691753/150‑1 (MQ=255)
                                                              ggCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGc                                                                                   <  2:1356781/150‑1 (MQ=255)
                                                                cATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGAGATCAAATGTCTGGTGGACAATCCGCAAAGCAACCAGGTCGCATTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTCAGTTCCTGAATGATGCCt                                                                                 >  1:524578/1‑150 (MQ=255)
                                                                 aTTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTa                                                                                <  2:1326295/150‑1 (MQ=255)
                                                                       tcatcaTTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgatga                                                                          >  1:1215152/1‑150 (MQ=255)
                                                                        catcatTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgatgat                                                                         >  1:1474879/1‑150 (MQ=255)
                                                                        catcatTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCAGCAAAGCAACCAGGTCGCGTTGCGCAATGGTGTTATCCTTGAAGCTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgatgat                                                                         >  1:869495/1‑150 (MQ=255)
                                                                          acatTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGTTCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGAtgt                                                                       <  1:1073999/149‑1 (MQ=255)
                                                                           catTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGAtgtg                                                                      >  2:1186600/1‑150 (MQ=255)
                                                                           catTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGGCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTAGGATGAtgtg                                                                      >  2:1707888/1‑150 (MQ=255)
                                                                           catTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGACGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGAtgtt                                                                      >  1:814508/1‑149 (MQ=255)
                                                                                 gCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTta                                                                >  1:312555/1‑150 (MQ=255)
                                                                                   ccAGGCTGGTGAACTTAGACGCTTCGTGAGCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata                                                              <  1:645712/150‑1 (MQ=255)
                                                                                    cAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAc                                                             >  1:408102/1‑150 (MQ=255)
                                                                                     aGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCACCCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcg                                                            >  2:1030306/1‑150 (MQ=255)
                                                                                         tGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcgcgcg                                                        <  2:1418819/150‑1 (MQ=255)
                                                                                            tGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtat                                                     <  2:1478550/150‑1 (MQ=255)
                                                                                            tGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtat                                                     >  1:1721405/1‑150 (MQ=255)
                                                                                            tGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtat                                                     >  2:414988/1‑150 (MQ=255)
                                                                                             gAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtatt                                                    >  2:642526/1‑150 (MQ=255)
                                                                                                cTTAGACGCTTCGTGATCAAATGTCGGGTGGACAAACCGCAAAGCAACCACGTCGCTAAGCGCAATGGTATTATCCTAAAAGGTTGCCTGAAACAGGCTCAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAAGCGCGTATTATc                                                 >  1:359650/1‑150 (MQ=255)
                                                                                                 ttAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGGCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCg                                                <  2:129270/150‑1 (MQ=255)
                                                                                                 attctcGCTTCGTGTAGCAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCg                                                <  1:233373/145‑1 (MQ=255)
                                                                                                     aCGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTc                                            <  2:1407178/150‑1 (MQ=255)
                                                                                                      cGCTTCGTGATCAAATGTCGGGTGGACAATACGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTATATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTTAACTTATACGCGCGTATTATCGATTca                                           >  2:923196/1‑150 (MQ=255)
                                                                                                       gCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTcac                                          <  1:1317807/150‑1 (MQ=255)
                                                                                                        cTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTcaca                                         <  1:394482/150‑1 (MQ=255)
                                                                                                        cTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTcaca                                         <  1:400724/150‑1 (MQ=255)
                                                                                                          tCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAAt                                       >  2:1013930/1‑150 (MQ=255)
                                                                                                            gTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGCTTTTATCATTGAAGGTTGCCTGAAACAGGTTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATaa                                     >  1:497555/1‑150 (MQ=255)
                                                                                                                  aaaTGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGc                               >  1:106674/1‑150 (MQ=255)
                                                                                                                  aaaTGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGc                               >  2:908352/1‑150 (MQ=255)
                                                                                                                   aaTGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGcc                              <  1:519538/150‑1 (MQ=255)
                                                                                                                    aTGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCa                             <  1:993018/150‑1 (MQ=255)
                                                                                                                     tGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAg                            <  1:1714630/150‑1 (MQ=255)
                                                                                                                     tGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAg                            <  1:1123468/150‑1 (MQ=255)
                                                                                                                           gTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGt                      <  2:459470/150‑1 (MQ=255)
                                                                                                                             ggACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCATTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATTATGTGAACTTATACGCGCGTATTATCGAGTCACAATAACCCTGCCAGCGGCGtta                    >  2:1285104/1‑149 (MQ=255)
                                                                                                                              gACAATCCGCAAAGCAACCAGGTCGCTTTGCGCATTGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTcg                   >  2:832043/1‑150 (MQ=255)
                                                                                                                               ccattgcGCAAAGCGACCTGGTTGCTTTGCGGAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTcgc                  >  1:1129329/7‑150 (MQ=255)
                                                                                                                                cattgcGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTcgcg                 >  2:760353/6‑150 (MQ=255)
                                                                                                                                 aaTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGt                >  2:1520397/1‑150 (MQ=255)
                                                                                                                                  aTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTa               >  2:419724/1‑150 (MQ=255)
                                                                                                                                     cGCAAAGCAACCAGGTCGCGTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATg            <  1:847723/150‑1 (MQ=255)
                                                                                                                                       cAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATgcg          >  1:974736/1‑150 (MQ=255)
                                                                                                                                           gCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGctcc      <  2:1551943/150‑1 (MQ=255)
                                                                                                                                           gCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGctcc      <  1:1565743/150‑1 (MQ=255)
                                                                                                                                              aCCAGGTCGCTTTGCGCAATGGTTTTATCCTTGCAGGTGGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCg   <  1:1442633/150‑1 (MQ=255)
                                                                                                                                               ccACGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGTCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGACAGCGGCGTTCGCGTAATGAGCTCCTCGc  <  1:353777/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
GAAATAGGCTACTGGCTGGACGAATCTCATCAGGGGCAGGGGATCATTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGC  >  NC_000913/1499220‑1499512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: