Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 138,662 T→G 5.8% intergenic (+29/+173) cueO → / ← gcd multicopper oxidase (laccase)/glucose dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913138,6620TG 5.8% 294.4 / 4.1 101intergenic (+29/+173)cueO/gcdmulticopper oxidase (laccase)/glucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (54/39);  new base G (4/3);  total (59/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

AATGTCAGCGAAGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACG  >  NC_000913/138514‑138812
                                                                                                                                                    |                                                                                                                                                      
aaTGTCAGCGAAGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGAtc                                                                                                                                                       <  1:1387314/150‑1 (MQ=255)
aaTGTCAGCGAAGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGAtc                                                                                                                                                       <  1:1354625/150‑1 (MQ=255)
   gTCAGCGAAGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGAtctct                                                                                                                                                    >  2:575180/1‑150 (MQ=255)
   gTCAGCGAAGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGAtctct                                                                                                                                                    >  2:213319/1‑150 (MQ=255)
   gTCAGCGAAGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGAtctct                                                                                                                                                    >  2:647144/1‑150 (MQ=255)
   gTCAGCGAAGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCCGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGAtctct                                                                                                                                                    >  2:1625827/1‑150 (MQ=255)
    tCAGCGAAGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGAtctctc                                                                                                                                                   >  1:961181/1‑150 (MQ=255)
     cAGCGAAGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTcc                                                                                                                                                  <  1:395795/150‑1 (MQ=255)
      aGCGAAGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCg                                                                                                                                                 >  2:774990/1‑150 (MQ=255)
         gAAGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGc                                                                                                                                              >  1:899425/1‑150 (MQ=255)
           aGTGCTGGTTAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCTCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGAGAGATCCGGGCAt                                                                                                                                            <  1:1031150/150‑1 (MQ=255)
           aGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCAt                                                                                                                                            <  2:706242/150‑1 (MQ=255)
                  gggatgTTTAATCACGATGCACCGAAAGAACCTGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTCCGGTATAAAAAGACCGTATTCGGAAATATGCCCGGAGCTCTCCGGGCATTAAACtg                                                                                                                                     <  1:1141828/145‑1 (MQ=255)
                    gAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACtgat                                                                                                                                   <  1:195407/150‑1 (MQ=255)
                    gAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACtgat                                                                                                                                   >  2:1066237/1‑150 (MQ=255)
                     aaGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATTCGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACtgatg                                                                                                                                  <  1:1235589/150‑1 (MQ=255)
                       gTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGAc                                                                                                                                <  1:1387850/150‑1 (MQ=255)
                         ttAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGCCCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACaa                                                                                                                              >  1:1649977/1‑150 (MQ=255)
                             tCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGAAAGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGAAGACAAACGc                                                                                                                          >  1:85891/1‑150 (MQ=255)
                              cACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCa                                                                                                                         >  2:1372857/1‑150 (MQ=255)
                                cGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCaaa                                                                                                                       >  2:1702451/1‑150 (MQ=255)
                                   tGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCt                                                                                                                    <  1:307616/150‑1 (MQ=255)
                                      aCCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGcc                                                                                                                 <  1:1391811/150‑1 (MQ=255)
                                       ccGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCt                                                                                                                <  1:1213498/150‑1 (MQ=255)
                                         gAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGa                                                                                                              >  2:466978/1‑150 (MQ=255)
                                         gAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATAAGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCTGAAATATGCCCGGAACTCTCCGGGCATTAAACTGATGACAAAAGCAAATCTGCATGa                                                                                                              >  2:466974/1‑150 (MQ=255)
                                               aCATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCt                                                                                                        <  1:178070/150‑1 (MQ=255)
                                                 aTGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTAc                                                                                                      <  1:1637972/150‑1 (MQ=255)
                                                    cTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCt                                                                                                   >  2:1596582/1‑150 (MQ=255)
                                                     ttATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCtt                                                                                                  >  1:1194212/1‑150 (MQ=255)
                                                      tataTGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCGTa                                                                                                 >  1:1506024/1‑150 (MQ=255)
                                                      tataTGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCAGTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTa                                                                                                 >  1:340719/1‑150 (MQ=255)
                                                        tatGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATc                                                                                               <  1:1573514/150‑1 (MQ=255)
                                                         atGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCa                                                                                              >  1:1329276/1‑150 (MQ=255)
                                                         atGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCa                                                                                              <  2:1699038/150‑1 (MQ=255)
                                                             cgcACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGcc                                                                                          >  1:375156/1‑150 (MQ=255)
                                                                aCTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTAc                                                                                       >  2:707400/1‑150 (MQ=255)
                                                                 cTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACg                                                                                      >  1:348229/1‑150 (MQ=255)
                                                                   gCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAACAGCCCGTATTCGGTAATATGCCCGGATATCTCGGGGCATTAAACTGATGAAAAAAGCAAATCTGCCTGATTCGCTACGCTTATCAGGCCTACGca                                                                                    >  1:272473/1‑149 (MQ=38)
                                                                     cATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAg                                                                                  >  2:855335/1‑150 (MQ=255)
                                                                     cATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAg                                                                                  >  1:205883/1‑150 (MQ=255)
                                                                      aTCTGCTGGAGCATGAAGATACTGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGc                                                                                 <  2:1501659/150‑1 (MQ=255)
                                                                       tCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCt                                                                                >  1:491872/1‑150 (MQ=255)
                                                                            tgGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCa                                                                           >  1:220996/1‑150 (MQ=255)
                                                                               aGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATc                                                                        <  1:1066237/150‑1 (MQ=255)
                                                                                gCATGAAGATACGGGGATGATGTTAGGGTTTACGTTATAAAAAGACCGTATTCGTAAATATGCCAGGATCTCTCCGCGCATAATACTGATGACAAACGCAAATCTGCATGATGAGCTAATCTTATAAGTCCAACGCTAGCTGTGCAATcc                                                                       >  2:335762/1‑150 (MQ=255)
                                                                                gCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATcc                                                                       <  2:879350/150‑1 (MQ=255)
                                                                                    gAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTg                                                                   <  2:1658514/150‑1 (MQ=255)
                                                                                    gAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTg                                                                   >  2:321302/1‑150 (MQ=255)
                                                                                    cacgATACCGCGATGATGGGAGGGATTACGGTCTAAAAAGACCGTAGTCGGAAATAAGCCCGGAGATCTCCGGGCATTAAACTGATGACAAACGCAAAGCTGCCTGATGCGCTACGCTTAACAGGCCTACGCTAGCTGTGCAATCCATTg                                                                   <  2:260355/147‑1 (MQ=25)
                                                                                        aTACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGAttt                                                               <  1:1206019/150‑1 (MQ=255)
                                                                                         tACGGGGATGATGTTAGGGTTTACGGTATAAAAAGCCCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGAtttt                                                              >  1:80761/1‑150 (MQ=255)
                                                                                         tACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGAtttt                                                              >  1:1290528/1‑150 (MQ=255)
                                                                                         tACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGAATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGAtttt                                                              <  1:25626/150‑1 (MQ=255)
                                                                                          aCGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTg                                                             >  1:1667303/1‑150 (MQ=255)
                                                                                           cGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGc                                                            >  2:1028710/1‑150 (MQ=255)
                                                                                            ggggATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGca                                                           >  2:1494724/1‑150 (MQ=255)
                                                                                              ggATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGcaca                                                         >  1:1566003/1‑150 (MQ=255)
                                                                                              ggATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGcaca                                                         >  2:705008/1‑150 (MQ=255)
                                                                                                atgatgTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAAt                                                       >  2:352073/1‑150 (MQ=255)
                                                                                                atgatgTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAAt                                                       >  2:1483303/1‑150 (MQ=255)
                                                                                                  gatgTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAAttt                                                     >  2:504305/1‑150 (MQ=255)
                                                                                                     gTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGt                                                  >  2:725521/1‑150 (MQ=255)
                                                                                                      ttAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGTATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTa                                                 <  2:936258/150‑1 (MQ=255)
                                                                                                      ttAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTa                                                 <  2:1194212/150‑1 (MQ=255)
                                                                                                      atAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCATTCCATTGATTTTGCACAATTTTGTa                                                 >  1:30078/2‑150 (MQ=255)
                                                                                                        aGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAgg                                               <  1:352073/150‑1 (MQ=255)
                                                                                                         gggTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGc                                              >  1:220457/1‑150 (MQ=255)
                                                                                                          ggTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCt                                             >  1:742804/1‑150 (MQ=255)
                                                                                                              tACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGAt                                         <  2:938046/150‑1 (MQ=255)
                                                                                                               aCGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATa                                        <  1:557412/150‑1 (MQ=255)
                                                                                                               aCGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATa                                        >  1:1475541/1‑150 (MQ=255)
                                                                                                                cGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATaa                                       >  1:240877/1‑150 (MQ=255)
                                                                                                                  gTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCTTTAAAATGATGACAAAAGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGATAGCTGTGAAATCCATTGATTTTGCACAATTTTGTAGGCAGGATAAgg                                     >  1:929260/1‑150 (MQ=37)
                                                                                                                    ataAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGTGCATTAAACTGATGACAATCGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCg                                   <  2:1213059/150‑1 (MQ=255)
                                                                                                                     taAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGt                                  >  2:1182998/1‑150 (MQ=255)
                                                                                                                         aaGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCAc                              <  1:1624192/150‑1 (MQ=255)
                                                                                                                           gACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCAcgc                            <  1:1625827/150‑1 (MQ=255)
                                                                                                                             ccGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCAcgccg                          <  1:647144/150‑1 (MQ=255)
                                                                                                                             ccGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCAcgccg                          >  2:581803/1‑150 (MQ=255)
                                                                                                                             ccGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCAcgccg                          <  1:575180/150‑1 (MQ=255)
                                                                                                                               gTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAg                                      >  1:1197133/1‑136 (MQ=255)
                                                                                                                               gTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAg                                      <  2:1197133/136‑1 (MQ=255)
                                                                                                                                 aTTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATc                      <  1:885591/150‑1 (MQ=255)
                                                                                                                                  ttCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATcc                     >  2:1522973/1‑150 (MQ=255)
                                                                                                                                  ttCGGAAATATGCCCGGAGCTCTCCGGGCATTAAACTGATGACAAGCGCAAATCTGCCTGGTGCGCTCCGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCCCGCCGCGTcc                     >  2:407176/1‑150 (MQ=37)
                                                                                                                                    cGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCgg                   >  2:1170529/1‑150 (MQ=255)
                                                                                                                                    cGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCgg                   <  2:961181/150‑1 (MQ=255)
                                                                                                                                     ggAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGc                  >  2:240074/1‑150 (MQ=255)
                                                                                                                                       aaaTATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATGCGGCGTGAACGCCTTATCCAGcct                <  2:909368/150‑3 (MQ=37)
                                                                                                                                       aaaTATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATGCGGCGTGAACGCCTTATCCAGcct                <  2:1222470/150‑3 (MQ=37)
                                                                                                                                        aaTATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCGGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATg               <  2:1456201/150‑1 (MQ=255)
                                                                                                                                           atGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTTATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGaac            <  2:1450368/150‑1 (MQ=255)
                                                                                                                                           atGCCCGGAGAGATCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGaac            >  1:1285446/1‑150 (MQ=255)
                                                                                                                                           atGCCCGGAGAGATCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGaac            >  1:497851/1‑150 (MQ=255)
                                                                                                                                             gCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATACGGCGTTCACGCCGCATCCGGCATGaacaa          >  1:476113/1‑150 (MQ=255)
                                                                                                                                             gCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAAATTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGaacaa          >  1:224976/1‑150 (MQ=255)
                                                                                                                                                    tctctcCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCCTCCGGCATGAACAACGCGCAc   >  2:1070200/1‑150 (MQ=255)
                                                                                                                                                    tctctcCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCacac   <  2:1290528/150‑1 (MQ=255)
                                                                                                                                                    tctctcCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACg  >  2:835181/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
AATGTCAGCGAAGTGCTGGTGAAGTTTAATCACGATGCACCGAAAGAACATGCTTATATGGCGCACTGCCATCTGCTGGAGCATGAAGATACGGGGATGATGTTAGGGTTTACGGTATAAAAAGACCGTATTCGGAAATATGCCCGGATCTCTCCGGGCATTAAACTGATGACAAACGCAAATCTGCCTGATGCGCTACGCTTATCAGGCCTACGCTAGCTGTGCAATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACG  >  NC_000913/138514‑138812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: