Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,520,584 A→G 5.9% D108G (GAC→GGC) ‡ mcbR → colanic acid and biofilm gene transcriptional regulator, MqsR‑controlled

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,520,5840AG 5.9% 346.8 / 9.1 119D108G (GAC→GGC) ‡mcbRcolanic acid and biofilm gene transcriptional regulator, MqsR‑controlled
Reads supporting (aligned to +/- strand):  ref base A (58/53);  new base G (4/3);  total (63/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01

Only 100 of 119 total aligned reads displayed.

GTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCA  >  NC_000913/1520436‑1520731
                                                                                                                                                    |                                                                                                                                                   
gTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGAc                                                                                                                                                    <  1:1337906/150‑1 (MQ=255)
  ttCGGTGAATGCGATTTCTGTCGCACCTGCACAATCATTTACAGTTCCGGAAGTGGGGAAAAGTCAATTAGACGAAATCATTCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCt                                                                                                                                                  <  1:56242/150‑1 (MQ=255)
     ggTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGATAACCTCACCCCGCAAGACCTTGc                                                                                                                                               <  2:234719/150‑1 (MQ=255)
     ggTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGc                                                                                                                                               <  1:946828/150‑1 (MQ=255)
     ggTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGc                                                                                                                                               >  1:1476151/1‑150 (MQ=255)
     ggTGAATGCGCTTTCTGTCGCACATGCACAAGCATTTTAAGTGCCGGAAGTGGGGCTACGTCAATTAGACGAAATCAATCGGATCCGCTACGATCTGGTATTAATGGCTGTTGATATTGCTGTTGACAACCTCACACCGCAAGAcgtacc                                                                                                                                               >  1:305616/1‑145 (MQ=255)
       tGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCgg                                                                                                                                             <  1:1650865/150‑1 (MQ=255)
         aaTGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGGTCTTGCgggg                                                                                                                                           <  1:1116163/150‑3 (MQ=255)
           tGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGGTCTTGCggggtg                                                                                                                                         <  2:1709389/150‑5 (MQ=255)
            gcgcTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTAAAAACCTCACCCCGCAAGACCTTGCGGAACTc                                                                                                                                        >  2:1295134/1‑150 (MQ=255)
             cgcTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTcc                                                                                                                                       <  2:300626/150‑1 (MQ=255)
               cTTTCTGTCGCACCTGCACAAGAATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGAAATTAATGGCAGTTGCTCTGGCTGTTGAACACCTCACCCCGCAAGACCTTGCGGCACTCCAg                                                                                                                                     >  1:273424/1‑150 (MQ=255)
                  gcTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGaa                                                                                                                                  <  2:1218228/149‑1 (MQ=255)
                     gTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGACCTCCAGGAATTg                                                                                                                               >  2:485381/1‑150 (MQ=255)
                       cGCACCTGCACATGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCATTTGCTCTGGATGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCt                                                                                                                             <  1:24558/150‑1 (MQ=255)
                        gCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTc                                                                                                                            >  2:830681/1‑150 (MQ=255)
                        gCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTc                                                                                                                            <  2:885404/150‑1 (MQ=255)
                           ccTGCACAAGCATTTACAGTTCCGGAAGTGGGGAACCGTGAATTAGCCGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCCCCCCGCAAGACCTTGCGGAACTCGAGGAATTGCTCgtg                                                                                                                         >  2:638693/1‑148 (MQ=255)
                           ccTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGCACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGACCTCCAGGAATTGCTCgag                                                                                                                         >  1:1364209/1‑150 (MQ=255)
                               cacaAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAAt                                                                                                                     >  2:1266109/1‑150 (MQ=255)
                                acaAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAAtt                                                                                                                    >  1:754737/1‑150 (MQ=255)
                                 caAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTa                                                                                                                   >  2:627725/1‑150 (MQ=255)
                                   aGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTAca                                                                                                                 <  2:418042/150‑1 (MQ=255)
                                    gCATTTACAGTTCCGGAAGTGGGGAAAAGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTAcag                                                                                                                <  1:75700/150‑1 (MQ=255)
                                     cATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTAcagc                                                                                                               >  1:895390/1‑150 (MQ=255)
                                      aTTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTAcagca                                                                                                              >  2:452519/1‑150 (MQ=255)
                                         tACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGgc                                                                                                           <  1:530289/150‑1 (MQ=255)
                                           cAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAGCTCCAGGAATTGCTCGAGAAATTACAGCAGgcgc                                                                                                         >  2:483198/1‑150 (MQ=255)
                                           cAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGgcgc                                                                                                         <  1:1395798/150‑1 (MQ=255)
                                            aGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCa                                                                                                        >  1:1673225/1‑150 (MQ=255)
                                            aGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGAACCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCa                                                                                                        >  1:712763/1‑150 (MQ=255)
                                             gTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCaa                                                                                                       >  1:773481/1‑150 (MQ=255)
                                                      gTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGc                                                                                              <  2:817546/150‑1 (MQ=255)
                                                       tGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTTCTCTGGCTGTTGAAATCCTCACCCCGCAACACCTTGCGGTAGTCCAGGAATTGCTCGTGATATTACAGCAGGCGCAAGAAAAGGGCg                                                                                             <  2:1030740/150‑1 (MQ=255)
                                                             aaCGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATgg                                                                                       >  2:656240/1‑150 (MQ=255)
                                                              aCGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGa                                                                                      <  1:1300154/150‑1 (MQ=255)
                                                                   aaTTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaaa                                                                                 <  2:895390/150‑1 (MQ=255)
                                                                      tAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCa                                                                              >  1:1180399/1‑150 (MQ=255)
                                                                       aGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCAt                                                                             <  2:529520/150‑1 (MQ=255)
                                                                        gACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCAtt                                                                            >  1:464703/1‑150 (MQ=255)
                                                                         aCGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACACCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTa                                                                           >  1:775901/1‑150 (MQ=255)
                                                                          cGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTaa                                                                          <  2:1355235/150‑1 (MQ=255)
                                                                          cGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTaa                                                                          >  1:601778/1‑150 (MQ=255)
                                                                            aaaTCAATCGGATCCGCTACGAACTGGAATTAATGTCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGTCGCAAGAAAAGGGCGACATGGAACAAATCATTAATg                                                                        <  1:1329757/150‑1 (MQ=255)
                                                                            aaaTCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATg                                                                        <  2:765090/150‑1 (MQ=255)
                                                                            aaaTCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATg                                                                        <  1:1377811/150‑1 (MQ=255)
                                                                                caatcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGCCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTaaa                                                                    >  1:921367/1‑150 (MQ=255)
                                                                                    cGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTAAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAgg                                                                <  1:1295134/150‑1 (MQ=255)
                                                                                      gATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCt                                                              <  2:1494888/150‑1 (MQ=255)
                                                                                           gCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTc                                                         <  1:515297/150‑1 (MQ=255)
                                                                                                gAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTa                                                    >  1:1103366/1‑150 (MQ=255)
                                                                                                   cTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCg                                                 <  1:911020/150‑1 (MQ=255)
                                                                                                    tGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGa                                                <  1:452519/150‑1 (MQ=255)
                                                                                                     ggAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAt                                               >  1:422683/1‑150 (MQ=255)
                                                                                                      gAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAtt                                              <  2:456131/150‑1 (MQ=255)
                                                                                                       aaTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAttt                                             >  2:1649148/1‑150 (MQ=255)
                                                                                                       aaTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAttt                                             <  2:1103366/150‑1 (MQ=255)
                                                                                                        aTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTa                                            >  1:422566/1‑150 (MQ=255)
                                                                                                          tAATGGCAGTTGCTCTGGCTGTTGAAAACCTCCCCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAAGTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatc                                          >  1:1376947/1‑150 (MQ=255)
                                                                                                            aTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatcat                                        >  1:1009492/1‑150 (MQ=255)
                                                                                                            aTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCAATTTCGCTTAGCGATTTatcat                                        >  1:1089967/1‑150 (MQ=255)
                                                                                                             tGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatcatc                                       <  2:1680368/150‑1 (MQ=255)
                                                                                                             tGGCAGTTGCTCAGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatcatc                                       <  1:266583/150‑1 (MQ=255)
                                                                                                                cAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGCACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCt                                    >  1:105942/1‑150 (MQ=255)
                                                                                                                 aGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTc                                   <  2:1476151/150‑1 (MQ=255)
                                                                                                                  gTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCa                                  >  2:1294497/1‑150 (MQ=255)
                                                                                                                    tGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCaaa                                <  1:10523/150‑1 (MQ=255)
                                                                                                                         tGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGc                           <  1:633655/150‑1 (MQ=255)
                                                                                                                          ggCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGcc                          <  2:1162422/150‑1 (MQ=255)
                                                                                                                          ggCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGcc                          <  1:830681/150‑1 (MQ=255)
                                                                                                                            cTGTTGAAAACCTCACCCCGCAAGACTTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCa                        >  2:744621/1‑150 (MQ=255)
                                                                                                                            cTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCa                        >  2:1640585/1‑150 (MQ=255)
                                                                                                                            cTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCa                        >  1:1247013/1‑150 (MQ=255)
                                                                                                                            cTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCCTAGCGATTTATCATCGCTCAAATATGCCCa                        >  1:1609854/1‑150 (MQ=255)
                                                                                                                                 gAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAACTCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtg                   >  1:1046704/1‑150 (MQ=255)
                                                                                                                                 gAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGTTTTATCATCGCTCAAATATGCCAATCCtg                   >  1:1603313/1‑150 (MQ=255)
                                                                                                                                 gAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtg                   <  2:1535818/150‑1 (MQ=255)
                                                                                                                                   aaaCCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtg                 <  1:485381/150‑1 (MQ=255)
                                                                                                                                   aaaCCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtg                 >  2:496970/1‑150 (MQ=255)
                                                                                                                                    aaCCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtgt                >  2:493273/1‑150 (MQ=255)
                                                                                                                                     aCCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAATCAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtgtg               <  2:790790/150‑1 (MQ=255)
                                                                                                                                     aCCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtgtg               >  1:1204426/1‑150 (MQ=255)
                                                                                                                                     aCCTCAACCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtgtg               <  2:516017/150‑1 (MQ=255)
                                                                                                                                      ccTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTga              <  2:3830/150‑1 (MQ=255)
                                                                                                                                      ccTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTga              >  1:724346/1‑150 (MQ=255)
                                                                                                                                      ccTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTga              >  1:676093/1‑150 (MQ=255)
                                                                                                                                        tCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgaga            <  2:1673225/150‑1 (MQ=255)
                                                                                                                                        gagttccGCAAGGTCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgaga            >  1:1198300/6‑150 (MQ=255)
                                                                                                                                          gttccGCAAGGTCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgatg          >  1:450225/4‑150 (MQ=255)
                                                                                                                                          gttccGCAAGGGCTAGCGGATCTCCAGGAATTGCTCGAGATATTACAGCAGGAGCAAGATAAGGGCGACATGGAACAAATCATTAAAGTAGACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTTAGTGAgatt          >  2:1540459/4‑149 (MQ=255)
                                                                                                                                           ccccGCAAGACCTTGCGGAACTCCAGGAATTGATCGAGAAATTACAGCAGGCCCAAGAAAAGGCCGACATCGAACAAATCATTAATGAAAACACGATATTTCGATAAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGAGAgatta         >  1:240663/1‑148 (MQ=255)
                                                                                                                                           ccacGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCACGCGCAAGAAAAGGGCGACATGGAACAAATCATTAAGGTAAACAGGCTATTTCGCTTAGCGAATTATCATCGCTCAAATATGCCGATCCTGTTTGtgatga         >  2:1130776/4‑150 (MQ=255)
                                                                                                                                            cccGCAAGCCCTGGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCCGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGCTTTATCATCGCTCAAATATGCCCATCCTGTGTGAgttgat        >  2:273642/1‑150 (MQ=255)
                                                                                                                                            cccGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgatgat        >  2:1029046/1‑150 (MQ=255)
                                                                                                                                             ccGCAAGACCTTGCGGAACTCCCGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTATTGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGAtt       <  2:250164/150‑1 (MQ=255)
                                                                                                                                               gCAAGGTCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTgc     >  1:1686119/1‑149 (MQ=255)
                                                                                                                                                cAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAg    <  1:99412/150‑1 (MQ=255)
                                                                                                                                                cAAGAACTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCGAGAAAAGGTCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAg    <  2:513066/150‑1 (MQ=255)
                                                                                                                                                  aGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGACCAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCa  <  2:273424/150‑1 (MQ=255)
                                                                                                                                                  aGACCTTGCGGAACTCCAGGAATTGCTCGAGAAAATACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCTCTCAAATATGCCCATCCTGTGTGAGATGATAGAGCa  >  1:519369/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCA  >  NC_000913/1520436‑1520731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: