Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,520,585 C→T 5.2% D108D (GAC→GAT) ‡ mcbR → colanic acid and biofilm gene transcriptional regulator, MqsR‑controlled

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,520,5850CT 5.2% 367.8 / 6.0 117D108D (GAC→GAT) ‡mcbRcolanic acid and biofilm gene transcriptional regulator, MqsR‑controlled
Reads supporting (aligned to +/- strand):  ref base C (58/51);  new base T (3/3);  total (62/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

Only 100 of 121 total aligned reads displayed.

GTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACT  >  NC_000913/1520436‑1520734
                                                                                                                                                     |                                                                                                                                                     
gTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGAc                                                                                                                                                       <  1:1337906/150‑1 (MQ=255)
  ttCGGTGAATGCGATTTCTGTCGCACCTGCACAATCATTTACAGTTCCGGAAGTGGGGAAAAGTCAATTAGACGAAATCATTCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCt                                                                                                                                                     <  1:56242/150‑1 (MQ=255)
     ggTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGATAACCTCACCCCGCAAGACCTTGc                                                                                                                                                  <  2:234719/150‑1 (MQ=255)
     ggTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGc                                                                                                                                                  >  2:1300154/1‑150 (MQ=255)
     ggTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGc                                                                                                                                                  <  1:946828/150‑1 (MQ=255)
     ggTGAATGCGCTTTCTGTCGCACATGCACAAGCATTTTAAGTGCCGGAAGTGGGGCTACGTCAATTAGACGAAATCAATCGGATCCGCTACGATCTGGTATTAATGGCTGTTGATATTGCTGTTGACAACCTCACACCGCAAGAcgtacc                                                                                                                                                  >  1:305616/1‑145 (MQ=255)
       tGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCgg                                                                                                                                                <  1:1650865/150‑1 (MQ=255)
         aaTGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGGTCTTGCgggg                                                                                                                                              <  1:1116163/150‑3 (MQ=255)
           tGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGGTCTTGCggggtg                                                                                                                                            <  2:1709389/150‑5 (MQ=255)
            gcgcTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTAAAAACCTCACCCCGCAAGACCTTGCGGAACTc                                                                                                                                           >  2:1295134/1‑150 (MQ=255)
             cgcTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTcc                                                                                                                                          <  2:300626/150‑1 (MQ=255)
              gcTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTAGCTGTTGAAAACCTCACCCCGCAAGGTCTTGCggggtgagg                                                                                                                                         <  1:1374941/150‑8 (MQ=255)
                tttCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAgg                                                                                                                                       >  1:516017/1‑150 (MQ=255)
                  tCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGaa                                                                                                                                     >  2:606832/1‑150 (MQ=255)
                  gcTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGaa                                                                                                                                     <  2:1218228/149‑1 (MQ=255)
                     gTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGACCTCCAGGAATTg                                                                                                                                  >  2:485381/1‑150 (MQ=255)
                       cGCACCTGCACATGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCATTTGCTCTGGATGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCt                                                                                                                                <  1:24558/150‑1 (MQ=255)
                        gCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTc                                                                                                                               >  2:830681/1‑150 (MQ=255)
                        gCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTc                                                                                                                               <  2:885404/150‑1 (MQ=255)
                           ccTGCACAAGCATTTACAGTTCCGGAAGTGGGGAACCGTGAATTAGCCGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCCCCCCGCAAGACCTTGCGGAACTCGAGGAATTGCTCgtg                                                                                                                            >  2:638693/1‑148 (MQ=255)
                           ccTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCgag                                                                                                                            >  2:1111279/1‑150 (MQ=255)
                                acaAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAAtt                                                                                                                       >  1:754737/1‑150 (MQ=255)
                                    gCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTAcag                                                                                                                   <  2:186069/150‑1 (MQ=255)
                                    gCATTTACAGTTCCGGAAGTGGGGAAAAGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTAcag                                                                                                                   <  1:75700/150‑1 (MQ=255)
                                     cATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTAcagc                                                                                                                  >  1:895390/1‑150 (MQ=255)
                                      aTTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTAcagca                                                                                                                 >  2:452519/1‑150 (MQ=255)
                                         tACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGgc                                                                                                              <  1:530289/150‑1 (MQ=255)
                                           cAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAGCTCCAGGAATTGCTCGAGAAATTACAGCAGgcgc                                                                                                            >  2:483198/1‑150 (MQ=255)
                                           cAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGgcgc                                                                                                            <  1:1395798/150‑1 (MQ=255)
                                            aGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGAACCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCa                                                                                                           >  1:712763/1‑150 (MQ=255)
                                                    agtgggggAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAgg                                                                                                   >  1:162677/5‑150 (MQ=255)
                                                      gTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGc                                                                                                 <  2:817546/150‑1 (MQ=255)
                                                       tGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTTCTCTGGCTGTTGAAATCCTCACCCCGCAACACCTTGCGGTAGTCCAGGAATTGCTCGTGATATTACAGCAGGCGCAAGAAAAGGGCg                                                                                                <  2:1030740/150‑1 (MQ=255)
                                                             aaCGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATgg                                                                                          >  2:656240/1‑150 (MQ=255)
                                                              aCGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGa                                                                                         <  1:1300154/150‑1 (MQ=255)
                                                                   aaTTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaaa                                                                                    <  2:895390/150‑1 (MQ=255)
                                                                      tAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCa                                                                                 >  1:1180399/1‑150 (MQ=255)
                                                                       aGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCAt                                                                                <  2:529520/150‑1 (MQ=255)
                                                                        gACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCAtt                                                                               >  1:464703/1‑150 (MQ=255)
                                                                         aCGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACACCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTa                                                                              >  1:775901/1‑150 (MQ=255)
                                                                          cGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTaa                                                                             >  1:601778/1‑150 (MQ=255)
                                                                          cGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTaa                                                                             <  2:1355235/150‑1 (MQ=255)
                                                                            aaaTCAATCGGATCCGCTACGAACTGGAATTAATGTCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGTCGCAAGAAAAGGGCGACATGGAACAAATCATTAATg                                                                           <  1:1329757/150‑1 (MQ=255)
                                                                            aaaTCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATg                                                                           <  1:1377811/150‑1 (MQ=255)
                                                                            aaaTCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATg                                                                           <  2:765090/150‑1 (MQ=255)
                                                                                    cGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTAAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAgg                                                                   <  1:1295134/150‑1 (MQ=255)
                                                                                     ggATCGGCTACGAACTGGAATTAATGGCAGTTGCTCGGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGc                                                                  >  1:925072/1‑150 (MQ=255)
                                                                                           gCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTc                                                            <  1:515297/150‑1 (MQ=255)
                                                                                              aCGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCt                                                         <  1:1254009/150‑1 (MQ=255)
                                                                                                 aaCTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAg                                                      <  1:692971/150‑1 (MQ=255)
                                                                                                   cTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCg                                                    <  1:911020/150‑1 (MQ=255)
                                                                                                    tGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGa                                                   <  1:452519/150‑1 (MQ=255)
                                                                                                     ggAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAt                                                  >  1:449222/1‑150 (MQ=255)
                                                                                                     ggAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAt                                                  >  1:422683/1‑150 (MQ=255)
                                                                                                      gAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAtt                                                 <  2:456131/150‑1 (MQ=255)
                                                                                                       aaTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAttt                                                >  2:1649148/1‑150 (MQ=255)
                                                                                                        aTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTa                                               >  1:422566/1‑150 (MQ=255)
                                                                                                          tAATGGCAGTTGCTCTGGCTGTTGAAAACCTCCCCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAAGTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatc                                             >  1:1376947/1‑150 (MQ=255)
                                                                                                            aTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatcat                                           >  1:1009492/1‑150 (MQ=255)
                                                                                                            aTGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCAATTTCGCTTAGCGATTTatcat                                           >  1:1089967/1‑150 (MQ=255)
                                                                                                             tGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatcatc                                          <  2:1680368/150‑1 (MQ=255)
                                                                                                             tGGCAGTTGCTCAGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatcatc                                          <  1:266583/150‑1 (MQ=255)
                                                                                                                cAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGCACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCt                                       >  1:105942/1‑150 (MQ=255)
                                                                                                                 aGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTc                                      <  2:1476151/150‑1 (MQ=255)
                                                                                                                  gTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCa                                     >  2:1294497/1‑150 (MQ=255)
                                                                                                                    tGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCaaa                                   <  1:10523/150‑1 (MQ=255)
                                                                                                                         tGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGc                              <  1:633655/150‑1 (MQ=255)
                                                                                                                          ggCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGcc                             <  1:830681/150‑1 (MQ=255)
                                                                                                                          ggCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGcc                             <  2:162677/150‑1 (MQ=255)
                                                                                                                          ggCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGcc                             <  2:1162422/150‑1 (MQ=255)
                                                                                                                           gCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGccc                            <  1:774405/150‑1 (MQ=255)
                                                                                                                            cTGTTGAAAACCTCACCCCGCAAGACTTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCa                           >  2:744621/1‑150 (MQ=255)
                                                                                                                            cTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCa                           >  2:1640585/1‑150 (MQ=255)
                                                                                                                            cTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCa                           >  2:356354/1‑150 (MQ=255)
                                                                                                                            cTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCCTAGCGATTTATCATCGCTCAAATATGCCCa                           >  1:1609854/1‑150 (MQ=255)
                                                                                                                                 gAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAACTCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtg                      >  1:1046704/1‑150 (MQ=255)
                                                                                                                                 gAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGTTTTATCATCGCTCAAATATGCCAATCCtg                      >  1:1603313/1‑150 (MQ=255)
                                                                                                                                 gAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtg                      <  2:1535818/150‑1 (MQ=255)
                                                                                                                                 gAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAGTTATCATCGACCAAATATGCACATCCtg                      >  1:254271/1‑150 (MQ=255)
                                                                                                                                  aaaaCCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgt                     <  2:773481/150‑1 (MQ=255)
                                                                                                                                   aaaCCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtg                    <  1:485381/150‑1 (MQ=255)
                                                                                                                                   aaaCCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtg                    >  2:496970/1‑150 (MQ=255)
                                                                                                                                    aaCCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAGTTATCATCGCTCAAATATGCCCATCCtgtgt                   >  2:486816/1‑150 (MQ=255)
                                                                                                                                     aCCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtgtg                  <  1:1111279/150‑1 (MQ=255)
                                                                                                                                     aCCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtgtg                  >  1:1204426/1‑150 (MQ=255)
                                                                                                                                     aCCTCAACCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtgtgtg                  <  2:516017/150‑1 (MQ=255)
                                                                                                                                      ccTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTga                 <  2:3830/150‑1 (MQ=255)
                                                                                                                                      ccTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTga                 >  1:724346/1‑150 (MQ=255)
                                                                                                                                        gagttccGCAAGGTCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgaga               >  1:1198300/6‑150 (MQ=255)
                                                                                                                                          gttccGCAAGGGCTAGCGGATCTCCAGGAATTGCTCGAGATATTACAGCAGGAGCAAGATAAGGGCGACATGGAACAAATCATTAAAGTAGACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTTAGTGAgatt             >  2:1540459/4‑149 (MQ=255)
                                                                                                                                           ccccGCAAGACCTTGCGGAACTCCAGGAATTGATCGAGAAATTACAGCAGGCCCAAGAAAAGGCCGACATCGAACAAATCATTAATGAAAACACGATATTTCGATAAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGAGAgatta            >  1:240663/1‑148 (MQ=255)
                                                                                                                                           ccacGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCACGCGCAAGAAAAGGGCGACATGGAACAAATCATTAAGGTAAACAGGCTATTTCGCTTAGCGAATTATCATCGCTCAAATATGCCGATCCTGTTTGtgatga            >  2:1130776/4‑150 (MQ=255)
                                                                                                                                            cccGCAAGCCCTGGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCCGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGCTTTATCATCGCTCAAATATGCCCATCCTGTGTGAgttgat           >  2:273642/1‑150 (MQ=255)
                                                                                                                                            cccGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgatgat           >  2:1029046/1‑150 (MQ=255)
                                                                                                                                             ccGCAAGACCTTGCGGAACTCCCGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTATTGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGAtt          <  2:250164/150‑1 (MQ=255)
                                                                                                                                               gCAAGGTCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTgc        >  1:1686119/1‑149 (MQ=255)
                                                                                                                                                cAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAg       <  1:99412/150‑1 (MQ=255)
                                                                                                                                                cAAGAACTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCGAGAAAAGGTCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAg       <  2:513066/150‑1 (MQ=255)
                                                                                                                                                     ccTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTTTGATCTATAAAATATGCCCATCCTGTGTGAGATGATTGAGAAACt  >  1:284124/1‑150 (MQ=255)
                                                                                                                                                     ccTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACt  >  1:1465892/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTTCGGTGAATGCGCTTTCTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACT  >  NC_000913/1520436‑1520734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: