Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,530,490 T→C 5.6% intergenic (+86/‑96) ydcD → / → yncI putative immunity protein for RhsE/pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,530,4900TC 5.6% 324.3 / 6.9 106intergenic (+86/‑96)ydcD/yncIputative immunity protein for RhsE/pseudogene
Reads supporting (aligned to +/- strand):  ref base T (49/51);  new base C (2/4);  total (51/55)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

Only 100 of 116 total aligned reads displayed.

TGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGAC  >  NC_000913/1530341‑1530637
                                                                                                                                                     |                                                                                                                                                   
tcttGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGTTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGTGACGGGGCAGGGCAAGAACATGAAAAtt                                                                                                                                                     >  1:199997/3‑150 (MQ=255)
 gTTTATTATTATGATAACAATTTCAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATCAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATtg                                                                                                                                                    <  2:876123/150‑1 (MQ=255)
 gTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATtg                                                                                                                                                    <  2:494140/150‑1 (MQ=255)
  ttGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATtgt                                                                                                                                                   <  1:1048030/150‑1 (MQ=255)
   tGATTATTATGATAACAATTTTAAAAATGAATTTTTTGACTGGATTGGTAATTGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATtgtg                                                                                                                                                  <  2:129057/150‑1 (MQ=255)
   tGATTATTATGATAACAATTTTAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACACAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATtgtg                                                                                                                                                  <  2:1532159/150‑1 (MQ=255)
   tGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCATGGCAAGATCATGAAAATtgtg                                                                                                                                                  >  2:1107915/1‑150 (MQ=255)
   tGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATtgtg                                                                                                                                                  <  2:1338813/150‑1 (MQ=255)
   tGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGGCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATtgtg                                                                                                                                                  <  2:952482/150‑1 (MQ=255)
   tGATTATTATCAAAACAATTTGAAAAATGAAGTTTTTGACTGGATTGTTAAATGGTCAAAATGAACAACACCAGAAAGAGAAAAATATAAAATACATCCAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATtgtg                                                                                                                                                  <  1:180392/150‑1 (MQ=255)
     attattATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGAt                                                                                                                                                <  1:3252/150‑1 (MQ=255)
              aTAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCATAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCAc                                                                                                                                       <  2:2900/150‑1 (MQ=255)
                aaCAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGa                                                                                                                                     >  1:1733452/1‑150 (MQ=255)
                 aCAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGATAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAAt                                                                                                                                    <  2:174681/150‑1 (MQ=255)
                  cAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGAtt                                                                                                                                   >  1:108401/1‑150 (MQ=255)
                     tttGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTc                                                                                                                                <  1:517249/150‑1 (MQ=255)
                     tttGAAAAATGAAGTTTTTGACTGGATTGGAAATTGGTCAAAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAATTTTc                                                                                                                                <  2:341938/150‑1 (MQ=255)
                       tcaaaaaTGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAATTTTCAt                                                                                                                              <  1:712491/148‑1 (MQ=255)
                         aaaaaTGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCt                                                                                                                            >  2:1273167/1‑150 (MQ=255)
                             aTGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAATAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGc                                                                                                                        <  2:1729363/150‑1 (MQ=255)
                                aaGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATTAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGATAATTGTGATGTAAATCACGATTTTCATCTTTGCttt                                                                                                                     >  2:159945/1‑150 (MQ=255)
                                 aGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTa                                                                                                                    <  2:740905/150‑1 (MQ=255)
                                   tttttGACTGGATTGGTAAATGGTCATAATTAACAACACCAGAAAGAGAAAAAAATAAAATACATACACAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAAc                                                                                                                  <  1:880975/150‑1 (MQ=255)
                                    ttttGACTGCATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATTTAAAATACATACAGAAAGACATGAGAGAAAGGTACGGGGCAGGGCAAGATCATCAAAATTGTGATCTAAATCACGATTTTCATCTTTGCTTTAACg                                                                                                                 >  2:887720/1‑150 (MQ=255)
                                     tttGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGc                                                                                                                <  2:545752/150‑1 (MQ=255)
                                      ttGACTGGATTGGTAAATGGTCACAATGAACAACACCAGAAAGAGAAAAATATAAAATACATTCAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAGATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGcc                                                                                                               <  1:11727/150‑1 (MQ=255)
                                       tGACTGGATTCGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAACACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCt                                                                                                              <  2:965086/150‑1 (MQ=255)
                                        gACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTa                                                                                                             >  1:1190565/1‑150 (MQ=255)
                                        gACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTa                                                                                                             >  1:1640135/1‑150 (MQ=255)
                                         aCTGGATTGGTAAAAGGTCATAATCAACAACACCAGAAAGAGAAAAATATAAAAAACATACAGAAAGACATGAGAGCAAGGGCCGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTAc                                                                                                            <  1:140025/150‑1 (MQ=255)
                                           tGGATTGGTAAATGGTCATAATGAACAACACCATAAAGATAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAg                                                                                                          <  2:1177591/150‑1 (MQ=255)
                                            ggATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGTAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAgg                                                                                                         >  2:314187/1‑150 (MQ=255)
                                            ggATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACAAACAGAAAGAAATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAgg                                                                                                         <  2:293331/150‑1 (MQ=255)
                                             gATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGt                                                                                                        <  2:957142/150‑1 (MQ=255)
                                              aTTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAAAATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTg                                                                                                       >  1:1313285/1‑150 (MQ=255)
                                               ttGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATATTCATCTATGCTTTAACGCCTACAGgaga                                                                                                      >  1:1694060/1‑150 (MQ=255)
                                                 ggTAAATGGTCATAATGAACAACACCAGAAAGAGAAAATTATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACTATTTTCATCTTTGCTTTAACGCCTACAGGTGATc                                                                                                    >  1:6451/1‑150 (MQ=255)
                                                    aaaTGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCaaa                                                                                                 >  1:1717674/1‑150 (MQ=255)
                                                     aaTGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCaaaa                                                                                                <  1:391847/150‑1 (MQ=255)
                                                         gTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGCCGGGGCTGGGCAAGATCATGAAAATTGTGATGTAAATCAAGATTTTCATCTTTGCGTTAACGTCGACAGGTGATCAAAATTTa                                                                                            >  2:169580/1‑150 (MQ=255)
                                                         gTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTa                                                                                            >  2:1014294/1‑150 (MQ=255)
                                                           cATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAAc                                                                                          <  2:495694/150‑1 (MQ=255)
                                                           cATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAAc                                                                                          <  2:738792/150‑1 (MQ=255)
                                                           cATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAAc                                                                                          <  2:1704559/150‑1 (MQ=255)
                                                           aaaaaTGAACATCAACAGAAAGATAAAAATAAAAAAAACATACAGAAAGAAATGAGAGCAAGGGACGGGGCTGGGCAAGATCTTGTAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCATATTTTAAc                                                                                          <  2:814486/147‑1 (MQ=255)
                                                             tAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCt                                                                                        >  1:1331040/1‑150 (MQ=255)
                                                             tAATGAACAACACAAGAAATATAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCt                                                                                        <  2:1331040/150‑1 (MQ=255)
                                                              aaTGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCCTGAAAATTGTGATGTAAATCACGATTTACAACTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCtt                                                                                       >  1:279874/1‑150 (MQ=255)
                                                              aaTGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTATCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCtt                                                                                       >  1:314166/1‑150 (MQ=255)
                                                               aTGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGCGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCAGCGTTGCTTTAACGCCTACAGGTGATCAAAATTTAACAttt                                                                                      >  1:1039730/1‑150 (MQ=255)
                                                                        acCAGAAAGAGAAAAATATAAAATACATACATAAAGACATGAGAGCAAGTGACTGGGCAGGGAAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACt                                                                             <  1:264401/150‑1 (MQ=255)
                                                                          cAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTaa                                                                           >  2:589649/1‑150 (MQ=255)
                                                                            gAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTaaaa                                                                         >  2:1224988/1‑150 (MQ=255)
                                                                             aaaGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCAAGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTaaaaa                                                                        <  1:1152131/150‑1 (MQ=255)
                                                                              aaGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAg                                                                       <  1:1424952/150‑1 (MQ=255)
                                                                              aaGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAg                                                                       <  1:1448267/150‑1 (MQ=255)
                                                                               agagAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGa                                                                      <  2:1485708/150‑1 (MQ=255)
                                                                               agagAAAAATATAAAATACAAACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGa                                                                      <  2:75744/150‑1 (MQ=255)
                                                                                tagaAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGAt                                                                     <  1:1491185/149‑1 (MQ=255)
                                                                                  gaAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATaa                                                                   >  2:1252336/1‑150 (MQ=255)
                                                                                    aaaaTATAAAATACATACAGAAAGACATGAGAGCAAGGGACGAGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATaaaa                                                                 >  2:1470402/1‑150 (MQ=255)
                                                                                       atatAAAATACATACAGAAAGACATGAGAGCAAGTGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAg                                                              <  1:134676/150‑1 (MQ=255)
                                                                                          taAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGAc                                                           >  1:552042/1‑150 (MQ=255)
                                                                                                  atacaGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGTAATAACTAAAAAGATAAAAAAGCACGCCAGGTg                                                   >  2:1137541/1‑150 (MQ=255)
                                                                                                  atacaGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTg                                                   >  2:1256382/1‑150 (MQ=255)
                                                                                                   tacaGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGa                                                  >  1:1517646/1‑150 (MQ=255)
                                                                                                    acaGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAg                                                 >  2:389495/1‑150 (MQ=255)
                                                                                                          aGACATTAGAGAAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGTAATAACTAAAAAGATAATAAAGGACGCCAGGTGAGTATTCa                                           <  2:1119490/150‑1 (MQ=255)
                                                                                                          aGACATGAGAGCAAGGGACGGGGCTGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTATAACGCCTACAGGTGAACAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGACAGTATTCa                                           >  2:753240/1‑150 (MQ=255)
                                                                                                                gagaGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGttt                                     <  2:1385314/150‑1 (MQ=255)
                                                                                                                 agagCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTg                                    <  1:1156689/150‑1 (MQ=255)
                                                                                                                 agagCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTGTAACGCCTACAGGTGATCTACATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGGATTCAAAGTTTg                                    >  1:265122/1‑150 (MQ=255)
                                                                                                                  gagCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTTCTTTAACGCCTACAGGTTATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGc                                   <  2:1263191/150‑1 (MQ=255)
                                                                                                                   agCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCt                                  <  2:695870/150‑1 (MQ=255)
                                                                                                                    gCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAATGTTTGCtt                                 >  1:289107/1‑150 (MQ=255)
                                                                                                                     cAAGGGTCGGGGCAGGGCAAGATCATGAAAATTTTTATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGTTGATCAAAATTTAACCTTTGTAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTg                                <  1:475027/150‑1 (MQ=255)
                                                                                                                     cAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTg                                <  2:1733452/150‑1 (MQ=255)
                                                                                                                      aaGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGa                               >  1:794650/1‑150 (MQ=255)
                                                                                                                       aGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTTAt                              >  1:134448/1‑150 (MQ=255)
                                                                                                                       aGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGAt                              <  2:108401/150‑1 (MQ=255)
                                                                                                                       aGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGAt                              >  1:139749/1‑150 (MQ=255)
                                                                                                                            cGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTatat                         >  1:779809/1‑150 (MQ=255)
                                                                                                                              gggcagggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATAttt                       >  2:390634/1‑150 (MQ=255)
                                                                                                                                 cagggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAg                    <  2:555026/150‑1 (MQ=255)
                                                                                                                                   gggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTg                  >  2:1518749/1‑150 (MQ=255)
                                                                                                                                    ggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGa                 >  2:402556/1‑150 (MQ=255)
                                                                                                                                     gcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAc                >  1:1370996/1‑150 (MQ=255)
                                                                                                                                     gcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCGTTGCTTTAACGCCGACAGGTGATCAAAATTTAACCTTTGGAATAACAAAAAAGATAAAAACGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAc                >  1:380682/1‑150 (MQ=255)
                                                                                                                                       aaGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGCAATAACTAAAACGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAccc              <  2:683736/150‑1 (MQ=255)
                                                                                                                                        caaagaTGAAAATCGTGATTTACATCACAATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAcccc             >  2:926053/6‑150 (MQ=39)
                                                                                                                                        caaagaTGAAAATCGTGATTTACATCACAATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCAAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAcccc             >  2:824757/6‑150 (MQ=39)
                                                                                                                                         gATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCt            >  1:490671/1‑150 (MQ=255)
                                                                                                                                         gATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCt            >  1:1330248/1‑150 (MQ=255)
                                                                                                                                         gATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCt            >  1:1607746/1‑150 (MQ=255)
                                                                                                                                            cATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGat         >  2:578806/1‑150 (MQ=255)
                                                                                                                                            aaTGAAAATTGTTATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAATATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGat         <  1:609901/149‑1 (MQ=255)
                                                                                                                                              tGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGatat       <  2:1717674/150‑1 (MQ=255)
                                                                                                                                                aaaaTTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGCCCCCTGATATAc     >  1:603830/1‑150 (MQ=255)
                                                                                                                                                 aaaTTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGTCGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACg    <  2:199997/150‑1 (MQ=255)
                                                                                                                                                   aTTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGac  <  2:486618/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGAC  >  NC_000913/1530341‑1530637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: