Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 143,534 G→A 6.7% L252L (CTG→CTA) ‡ yadG → putative ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913143,5340GA 6.7% 325.8 / 8.2 106L252L (CTG→CTA) ‡yadGputative ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (39/59);  new base A (3/4);  total (43/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.89e-01

Only 100 of 109 total aligned reads displayed.

GCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGA  >  NC_000913/143386‑143683
                                                                                                                                                    |                                                                                                                                                     
gCCGCAATATCGGCATTATTCAACACGGTGAGCTGGGGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTgg                                                                                                                                                      <  2:827496/150‑1 (MQ=255)
 ccGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGt                                                                                                                                                     >  1:31022/1‑150 (MQ=255)
 ccGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGt                                                                                                                                                     >  1:702760/1‑150 (MQ=255)
   gCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCg                                                                                                                                                   <  2:582263/150‑1 (MQ=255)
        atCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGGAACCTTTATTCTCGAGCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACCAGTCGATAct                                                                                                                                              <  1:817273/150‑2 (MQ=255)
        atCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACCAGTCGATAct                                                                                                                                              <  1:311992/150‑2 (MQ=255)
        atCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCATTACCGAAGCTCGATGGCTATCAGTATCGACCAGTCGATAct                                                                                                                                              <  2:507596/150‑2 (MQ=255)
         tCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACcg                                                                                                                                             <  1:747021/150‑1 (MQ=255)
          cGGCATTATTCAAAACTGTGAGCTGGTTGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTTAAATCGGAAACCTTTATTCTCGATCTCTCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACcgc                                                                                                                                            <  1:76220/150‑1 (MQ=255)
           ggCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACcgcg                                                                                                                                           >  2:1219322/1‑150 (MQ=255)
            gCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGAATCGACAGGTCGATACCGCGa                                                                                                                                          >  1:885970/1‑150 (MQ=255)
             cATTATTCAACACTGTGAGCTGGTGGAAAATACCTCTATGAAGGCGCTGCTGGCAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACg                                                                                                                                        <  2:605265/150‑1 (MQ=255)
                tattCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCt                                                                                                                                      <  1:1187132/150‑1 (MQ=255)
                  ttCAACACGGTGAGGTGGTGGAAAATCCCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTgg                                                                                                                                    >  1:38260/1‑150 (MQ=255)
                    cAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGaa                                                                                                                                  >  2:8158/1‑150 (MQ=255)
                    cAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAACCCCGTTACCGAAGCTCGATCGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGaa                                                                                                                                  >  2:1118007/1‑150 (MQ=255)
                     aaCACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAg                                                                                                                                 <  2:1126999/150‑1 (MQ=255)
                      acacGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCCCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGt                                                                                                                                <  1:408669/150‑1 (MQ=255)
                           gTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAg                                                                                                                           <  2:807957/150‑1 (MQ=255)
                                cTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGtgctg                                                                                                                      <  2:577810/150‑1 (MQ=255)
                                 tggtggAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGtgctgc                                                                                                                     >  1:645458/1‑150 (MQ=255)
                                 tggtggAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGtgctgc                                                                                                                     >  1:43515/1‑150 (MQ=255)
                                   gtggAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGt                                                                                                                   >  1:210143/1‑150 (MQ=255)
                                     ggAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGa                                                                                                                 >  2:973615/1‑150 (MQ=255)
                                      gAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAg                                                                                                                >  1:55494/1‑150 (MQ=255)
                                        aaaTACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCa                                                                                                              <  1:607126/150‑1 (MQ=255)
                                           tACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAggg                                                                                                           <  1:1671324/150‑1 (MQ=255)
                                           tACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAggg                                                                                                           >  2:452484/1‑150 (MQ=255)
                                           tACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCACTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAggg                                                                                                           <  2:1038924/150‑1 (MQ=255)
                                             ccTCGATGAAGGCGCTGCTGGCGAAGCTGAAGTCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGa                                                                                                         >  2:203101/1‑150 (MQ=255)
                                                cGATGAAGGCGCTGCTGGCGAGGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCa                                                                                                      <  1:1122087/150‑1 (MQ=255)
                                                cGATGAAGGCGCTGCTGGCGAAGCTGAAATCTGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCa                                                                                                      <  2:693943/150‑1 (MQ=255)
                                                cGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCa                                                                                                      <  2:31022/150‑1 (MQ=255)
                                                cGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCa                                                                                                      >  2:1429528/1‑150 (MQ=255)
                                                        gcgcTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTa                                                                                              <  2:359534/150‑1 (MQ=255)
                                                           ctgctgGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTAttt                                                                                           <  2:1426283/150‑1 (MQ=255)
                                                             gctgGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTAc                                                                                         >  1:370428/1‑150 (MQ=255)
                                                             gctgGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTAc                                                                                         >  1:1491274/1‑150 (MQ=255)
                                                              ctgGCGAAGCGGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACg                                                                                        <  2:1289700/150‑1 (MQ=255)
                                                               tgGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGc                                                                                       <  2:171482/150‑1 (MQ=255)
                                                               gggCGAAGCTGAAATCGGAAACCTTTATGCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGc                                                                                       <  1:1534439/149‑1 (MQ=255)
                                                                ggCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGCTGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCa                                                                                      >  1:476729/1‑150 (MQ=255)
                                                                 gCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAg                                                                                     <  1:920705/150‑1 (MQ=255)
                                                                  cGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGt                                                                                    >  2:1443242/1‑150 (MQ=255)
                                                                  cGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGAGCAACAGCGTATTTACGCAGt                                                                                    <  1:435547/150‑1 (MQ=255)
                                                                     aGCTGAAATCGGAAACCTTTATTCTCGATCTCGCCCCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTaa                                                                                 <  2:1536065/150‑1 (MQ=255)
                                                                     aGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTaa                                                                                 <  2:1411252/150‑1 (MQ=255)
                                                                      gCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAg                                                                                <  1:1516933/150‑1 (MQ=255)
                                                                       cTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTTCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGt                                                                               >  2:1187659/1‑150 (MQ=255)
                                                                        tGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTg                                                                              <  1:1118007/150‑1 (MQ=255)
                                                                        tGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACCAGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTg                                                                              >  2:374438/1‑150 (MQ=255)
                                                                         gAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGa                                                                             >  2:812323/1‑150 (MQ=255)
                                                                          aaaaCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAg                                                                            >  1:1698623/1‑150 (MQ=255)
                                                                           aaTCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGc                                                                           >  2:407658/1‑150 (MQ=255)
                                                                               ggAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAggg                                                                       <  1:1027900/150‑1 (MQ=255)
                                                                               ggAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAggg                                                                       <  1:1326896/150‑1 (MQ=255)
                                                                                gAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGc                                                                      <  1:25598/150‑1 (MQ=255)
                                                                                 aaaCCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTAGCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTTAGTGAGCAGGGCa                                                                     <  1:1322276/150‑1 (MQ=255)
                                                                                  aaCCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCAt                                                                    >  2:70867/1‑150 (MQ=255)
                                                                                   aCCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACAGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGAATTTACGCCGTTAAGTGAGCAGGGCAtt                                                                   >  1:269047/1‑150 (MQ=255)
                                                                                    ccTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTc                                                                  <  1:741951/150‑1 (MQ=255)
                                                                                     cTTTATTCTCGCTCTCGCACCGAAAAGCCAGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCagaca                                                                 >  2:34582/1‑146 (MQ=255)
                                                                                     cTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCa                                                                 >  1:1503084/1‑150 (MQ=255)
                                                                                     cTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCa                                                                 <  2:702760/150‑1 (MQ=255)
                                                                                         aTTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTa                                                             <  2:963371/150‑1 (MQ=255)
                                                                                           tctcGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGCCTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTAtt                                                           >  1:1518588/1‑150 (MQ=255)
                                                                                            ctcGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTa                                                          <  1:1654503/150‑1 (MQ=255)
                                                                                              cGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTATGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAg                                                        <  2:1657861/150‑1 (MQ=255)
                                                                                              cGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAg                                                        <  2:645458/150‑1 (MQ=255)
                                                                                              cGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAg                                                        <  2:43515/150‑1 (MQ=255)
                                                                                               gATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGt                                                       <  2:1515168/150‑1 (MQ=255)
                                                                                                  ctcGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATg                                                    <  2:765053/150‑1 (MQ=255)
                                                                                                    cGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCg                                                  <  1:900881/150‑1 (MQ=255)
                                                                                                     gCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGt                                                 >  1:1557093/1‑150 (MQ=255)
                                                                                                            aaaGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAg                                          >  1:926917/1‑150 (MQ=255)
                                                                                                            aaaGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAg                                          <  1:1431249/150‑1 (MQ=255)
                                                                                                              aGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCt                                        <  1:973615/150‑1 (MQ=255)
                                                                                                                cccGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTaa                                      <  1:567197/150‑1 (MQ=255)
                                                                                                                 ccGTTACCGACGCTCGATGGCTATCAGTATCGACTGGTCGATACCGAGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAAc                                     <  1:249227/150‑1 (MQ=255)
                                                                                                                  cGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAAcc                                    >  1:1186637/1‑150 (MQ=255)
                                                                                                                      aCCGAAGCTCGATGGCTATCAGTATGGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCt                                <  1:392970/150‑1 (MQ=255)
                                                                                                                      aCCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCt                                >  1:952523/1‑150 (MQ=255)
                                                                                                                        cGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTgg                              >  1:1340299/1‑150 (MQ=255)
                                                                                                                        cGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTgg                              <  2:1688732/150‑1 (MQ=255)
                                                                                                                           aGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAag                           <  1:582810/150‑1 (MQ=255)
                                                                                                                           aGCTCGATGGCTATCAGAATCGAAACGTCGATACCGCGACGCTGGAAGTTGAAGTGCAGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTGAGGTATTAAGAATGCGAAACAAAGCTAACCGACTGGaaa                           >  2:1118112/1‑149 (MQ=255)
                                                                                                                             cTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAagag                         <  2:1491274/150‑1 (MQ=255)
                                                                                                                              tCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGCGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGc                        <  1:1649521/150‑1 (MQ=255)
                                                                                                                                 aTGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCtgt                     <  1:1443242/150‑1 (MQ=255)
                                                                                                                                  tGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCtgtt                    <  1:1205449/150‑1 (MQ=255)
                                                                                                                                    gCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCtgtttg                  <  1:29881/150‑1 (MQ=255)
                                                                                                                                      tATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCtgtttgtt                >  1:471499/1‑150 (MQ=255)
                                                                                                                                         cggTATCGCCCAGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGAATTTACGCAGTTAAGTGAGCAGGGCATTAAGGTATTAAGTATGGGTAACAAAGCTAACAGTCTGGAAGAGCTgtttgtttgc             >  2:136407/3‑148 (MQ=255)
                                                                                                                                          ggTATCGACCAGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCAc            >  1:1246823/2‑150 (MQ=255)
                                                                                                                                          aGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCAc            >  2:1164016/1‑150 (MQ=255)
                                                                                                                                               cGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGtt       >  2:1457538/1‑150 (MQ=255)
                                                                                                                                                   tGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATg   <  1:420414/150‑1 (MQ=255)
                                                                                                                                                   tGGTCGATACCGCGACGCTGGAAGTTGAAGTGCGGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATg   <  2:1020748/150‑1 (MQ=255)
                                                                                                                                                    ggTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCTGGGGATCAACAGCGTATTTACGCAGGTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGa  <  1:598453/150‑1 (MQ=255)
                                                                                                                                                    ggTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGa  >  1:1699408/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATGAAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGCCCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTTGAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATTCAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTTAATGA  >  NC_000913/143386‑143683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: