Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,555,781 G→C 6.7% intergenic (‑112/+45) maeA ← / ← sra malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme/stationary‑phase‑induced ribosome‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,555,7810GC 6.7% 404.6 / 17.5 136intergenic (‑112/+45)maeA/sramalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme/stationary‑phase‑induced ribosome‑associated protein
Reads supporting (aligned to +/- strand):  ref base G (65/59);  new base C (4/5);  total (70/66)
Fisher's exact test for biased strand distribution p-value = 7.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.71e-01

Only 100 of 139 total aligned reads displayed.

ATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCC  >  NC_000913/1555632‑1555930
                                                                                                                                                       |                                                                                                                                                     
atatAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTgcg                                                                                                                                                       >  1:931163/1‑150 (MQ=255)
  ataAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTATGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCa                                                                                                                                                     >  1:1720192/1‑150 (MQ=255)
   taAAGCGAACGCTGTTTTTTTGTTTTTGTTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGTGTGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAg                                                                                                                                                    <  1:322711/150‑1 (MQ=255)
   taAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAg                                                                                                                                                    >  2:815389/1‑150 (MQ=255)
   taAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAg                                                                                                                                                    >  2:1174371/1‑150 (MQ=255)
    aaaGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAgg                                                                                                                                                   <  2:1420294/150‑1 (MQ=255)
    aaaGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAgg                                                                                                                                                   >  2:1510525/1‑150 (MQ=255)
      aGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCa                                                                                                                                                 >  2:862568/1‑150 (MQ=255)
           aCGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGgt                                                                                                                                            <  2:931163/150‑1 (MQ=255)
            cGCTGTTTTTTTGTTTTTTGTTCCATGTCACTCACTCTTTTTTGAAAATCCATCACT‑‑GGGGGGCTTTTATGGTCTTTGCTTAACCGCAAGGGCGGCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGgtg                                                                                                                                           <  2:258145/150‑1 (MQ=255)
            cGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGgtg                                                                                                                                           <  2:53296/150‑1 (MQ=255)
            cGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGgtg                                                                                                                                           <  2:1641770/150‑1 (MQ=255)
             gCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGgtgt                                                                                                                                          <  2:1435425/150‑1 (MQ=255)
              cTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGtt                                                                                                                                         <  2:1146526/150‑1 (MQ=255)
              cTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTGTTATCGTCTTTCCTTTACCGCCAGGGCGTCGGCCTCAAGTAGAAAGCAGATAAAAACAAAAACACCCTTGCGCAGGCAATGgtgat                                                                                                                                         >  2:752588/1‑148 (MQ=255)
              cTGTCTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGtt                                                                                                                                         <  2:1677133/150‑1 (MQ=255)
               tGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGttt                                                                                                                                        <  2:353751/150‑1 (MQ=255)
                   tttttgtttttgGTTCCATGTCACTCCCTCTTTTTTGAACATCCATCCCT‑‑GGGGGGCTTTTATCGTATTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGGCGtttttgt                                                                                                                                    <  2:1541019/150‑5 (MQ=255)
                   tttttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGGTGtttttgt                                                                                                                                    <  1:1375353/150‑5 (MQ=255)
                    ttttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCg                                                                                                                                   <  2:1123601/150‑1 (MQ=255)
                     tttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGGGCAATGGTGtttttgttt                                                                                                                                  <  2:408253/150‑7 (MQ=255)
                        gtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCAt                                                                                                                               >  1:1274331/1‑150 (MQ=255)
                        gtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCAt                                                                                                                               >  2:920030/1‑150 (MQ=255)
                         tttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCAACCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCGCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCAtt                                                                                                                              >  2:1699251/1‑150 (MQ=255)
                            ttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCCGGGCGGCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAg                                                                                                                           <  2:659481/150‑1 (MQ=255)
                             tgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAgg                                                                                                                          <  2:340641/150‑1 (MQ=255)
                             tgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAgg                                                                                                                          <  1:1605861/150‑1 (MQ=255)
                             tgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAgg                                                                                                                          <  1:499811/150‑1 (MQ=255)
                                  ccATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGa                                                                                                                     <  1:1313764/150‑1 (MQ=255)
                                   cATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGAt                                                                                                                    >  2:1436952/1‑150 (MQ=255)
                                   cATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTAATTGAGGACTGAt                                                                                                                    >  2:1032046/1‑150 (MQ=255)
                                        cactcactCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTa                                                                                                               >  1:911026/1‑150 (MQ=255)
                                        cactcactCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTa                                                                                                               >  2:1004983/1‑150 (MQ=255)
                                           acattattttttGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGa                                                                                                            >  2:474893/7‑150 (MQ=255)
                                             actcTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTAAATCGTCATTGACGACTGATGGTTATGAAt                                                                                                          >  2:1142742/1‑150 (MQ=255)
                                             actcTTTTTTGAATATCCATCCAT‑‑TGGGGGCTTTTATCGTCTTTGATTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAAAACCATTTCGCATGCTTTGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAAt                                                                                                          <  1:881427/150‑1 (MQ=255)
                                               tctTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTa                                                                                                        >  2:1593604/1‑150 (MQ=255)
                                                ctTTTTTGAATATCCCTCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCATCGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCAATGCGCAGGCAAAGGTGTTTAATCGACATTGAGGACTGATGGTTATGAATTAc                                                                                                       >  2:1233212/1‑150 (MQ=255)
                                                 ttttttGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCTTGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACt                                                                                                      <  2:1565871/150‑1 (MQ=255)
                                                  tttttGAATATCCATCCCTGGGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTAc                                                                                                       <  2:610413/150‑1 (MQ=255)
                                                    tttGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTTCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACtttt                                                                                                   >  1:1667646/1‑150 (MQ=255)
                                                         atatCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCg                                                                                              >  1:1263373/1‑150 (MQ=255)
                                                          tatCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCgg                                                                                             >  2:1303203/1‑150 (MQ=255)
                                                           atccatccCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCggg                                                                                            >  2:1648910/1‑150 (MQ=255)
                                                            tccatccCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCgggg                                                                                           <  1:1296723/150‑1 (MQ=255)
                                                             ccatccCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGc                                                                                          >  2:843456/1‑150 (MQ=255)
                                                              catccCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGAATGATGGTTATGAATTACTTTTCAGCGGTGCg                                                                                         >  2:1331144/1‑150 (MQ=255)
                                                                tccCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGtt                                                                                       <  2:1605393/150‑1 (MQ=255)
                                                                 cccT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGttt                                                                                      <  1:850686/150‑1 (MQ=255)
                                                                  ccT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGtttt                                                                                     <  2:1659272/150‑1 (MQ=255)
                                                                   cT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTc                                                                                    <  1:1004983/150‑1 (MQ=255)
                                                                    t‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCGAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCt                                                                                   <  2:666891/150‑1 (MQ=255)
                                                                    t‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCt                                                                                   <  2:240817/150‑1 (MQ=255)
                                                                        gggggCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGc                                                                                 <  1:1127614/150‑1 (MQ=255)
                                                                         ggggCTTTTATCGTCGTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGAAGGCAATGGTGTTTAATCGTCATTGAGTACTGATGGTTATGAATTACTTTTCATCGGGGCGTTTTATGcc                                                                                >  1:1267603/1‑150 (MQ=255)
                                                                         ggggCTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCg                                                                               <  1:1656150/150‑1 (MQ=255)
                                                                             cTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAATCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCAGGtt                                                                            >  1:698255/1‑150 (MQ=255)
                                                                             cTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTg                                                                                  <  2:1037433/144‑1 (MQ=255)
                                                                              ttttATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAAGAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTg                                                                           <  1:1501707/150‑1 (MQ=255)
                                                                               tttATAGTCTATGCTTTACAGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCATGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCTGGGCGTTTTCTGCCGGTTgg                                                                          <  1:291435/150‑1 (MQ=255)
                                                                                ttATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTggg                                                                         <  1:1062063/150‑1 (MQ=255)
                                                                                 tATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGt                                                                        <  1:474893/150‑1 (MQ=255)
                                                                                      tCTTTGCTTTACCGCCCGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAACACCCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAttt                                                                   <  1:1032874/150‑1 (MQ=255)
                                                                                      tCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAttt                                                                   >  2:850510/1‑150 (MQ=255)
                                                                                       cTTTGCTTTACCGCCAGGGCGTCGGACTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCGGCGGGGCGTTTTCTGCCGGTTGGGTTTTTta                                                                  >  2:97923/1‑150 (MQ=255)
                                                                                        tttGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTCCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTtac                                                                 <  2:185146/150‑1 (MQ=255)
                                                                                            cTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACg                                                             >  2:974541/1‑150 (MQ=255)
                                                                                                 ccGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGGGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTTTGAATTTCTTTTCAGCGGGGCGTTTTCGGCCGGTTGGGTTATTTACTACGCTGGa                                                        >  1:870293/1‑150 (MQ=255)
                                                                                                       gggCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTc                                                  <  1:328399/150‑1 (MQ=255)
                                                                                                             cGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTc                                            <  1:1135796/150‑1 (MQ=255)
                                                                                                               gCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCgg                                          <  1:194268/150‑1 (MQ=255)
                                                                                                                ccTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGt                                         >  1:1417993/1‑150 (MQ=255)
                                                                                                                 cTCAAGTATAAAGCAGATAAAAACAAAAACACCATAGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTCATGGTTATCAAATACTTTTCAGCGGGGCGTTTTCTGACTGTTGGGTTATTTACTACGATGGATTTGTCAACTTCGGTa                                        >  1:284214/1‑150 (MQ=255)
                                                                                                                         taAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGTCGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAAATAtttt                                >  2:289374/1‑150 (MQ=255)
                                                                                                                          aaaGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTg                               <  1:998386/150‑1 (MQ=255)
                                                                                                                          aaaGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTg                               >  1:334792/1‑150 (MQ=255)
                                                                                                                           aaGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTgc                              <  2:1097994/150‑1 (MQ=255)
                                                                                                                             gCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCTTTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTgcgc                            >  1:178886/1‑150 (MQ=255)
                                                                                                                              cAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCt                           <  1:1510525/150‑1 (MQ=255)
                                                                                                                               aGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTg                          >  2:363080/1‑150 (MQ=255)
                                                                                                                               aGATAAAAACAAAAACACCATTGCGAAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCTCTg                          >  1:42545/1‑150 (MQ=255)
                                                                                                                                   aaaaacaaaaacACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGCCTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTa                      >  2:79926/1‑150 (MQ=255)
                                                                                                                                   aaaaacaaaaacACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGTGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTa                      <  2:1226251/150‑1 (MQ=255)
                                                                                                                                   aaaaacaaaaacACCATTGCACAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTa                      <  1:480187/150‑1 (MQ=255)
                                                                                                                                    aaaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGTCTGATGGTTATGAATTACTTTTCATCGGGGCGTTTTCTGCCGGTTTGGTTATTTACTACGCTTGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTat                     <  1:451344/150‑2 (MQ=255)
                                                                                                                                    aaaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAg                     <  2:1074998/150‑1 (MQ=255)
                                                                                                                                     aaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGa                    >  2:626156/1‑150 (MQ=255)
                                                                                                                                     aaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGATTTCTGCAGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGa                    >  1:1160949/1‑150 (MQ=255)
                                                                                                                                      aacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGaa                   >  2:1399993/1‑150 (MQ=255)
                                                                                                                                      aacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTCATGGTTATGAATTACATTTCAGCGGGGAGTTTTCTGCCGGTTGGGTTATTTACTACGCTTTATTTGTCACCTTCGGTAACTATTTTGCGATGGTTAGaa                   >  2:1016169/1‑150 (MQ=255)
                                                                                                                                           aaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAtttt              <  1:815389/150‑1 (MQ=255)
                                                                                                                                            aacaCCATTGCCTGCGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTa             >  2:428351/1‑150 (MQ=255)
                                                                                                                                            aacaCCATTGCCTGCGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTAGTTTGCGCTGGTTAGAAATTTTa             >  1:1160361/1‑150 (MQ=255)
                                                                                                                                            aacaCCATTGCCTACGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTa             >  2:1397317/1‑150 (MQ=255)
                                                                                                                                             acacCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCAGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTAt            >  2:121122/1‑150 (MQ=255)
                                                                                                                                              cacCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATg           <  1:1019703/150‑1 (MQ=255)
                                                                                                                                                ccATTGCGCAGGCAATGGTGTTTAATCGTCAGTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgt         >  2:206832/1‑150 (MQ=255)
                                                                                                                                                 cATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtc        >  2:571563/1‑150 (MQ=255)
                                                                                                                                                  aTTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtcc       <  1:1370018/150‑1 (MQ=255)
                                                                                                                                                       gcAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtccagtcc  <  2:911026/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
ATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCC  >  NC_000913/1555632‑1555930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: