Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,555,782 C→T 6.6% intergenic (‑113/+44) maeA ← / ← sra malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme/stationary‑phase‑induced ribosome‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,555,7820CT 6.6% 408.4 / 10.8 136intergenic (‑113/+44)maeA/sramalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme/stationary‑phase‑induced ribosome‑associated protein
Reads supporting (aligned to +/- strand):  ref base C (64/61);  new base T (4/5);  total (70/66)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.28e-01

Only 100 of 138 total aligned reads displayed.

ATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCC  >  NC_000913/1555634‑1555930
                                                                                                                                                      |                                                                                                                                                    
 taAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAg                                                                                                                                                    >  2:815389/1‑150 (MQ=255)
 taAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAg                                                                                                                                                    >  2:1174371/1‑150 (MQ=255)
 taAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAg                                                                                                                                                    >  2:1019703/1‑150 (MQ=255)
  aaaGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAgg                                                                                                                                                   >  2:1510525/1‑150 (MQ=255)
  aaaGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAgg                                                                                                                                                   <  2:1420294/150‑1 (MQ=255)
  aaaGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATACATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAATCAGATAAAAACAAAAACACCATTGCGCAgg                                                                                                                                                   >  2:1205973/1‑150 (MQ=255)
    aGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCa                                                                                                                                                 >  2:862568/1‑150 (MQ=255)
        aaCGCTGTTTTTTTGGTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCCTCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCACGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATgg                                                                                                                                             <  2:1000452/150‑1 (MQ=255)
         aCGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGgt                                                                                                                                            <  2:931163/150‑1 (MQ=255)
         aCGCTGTTTTTTTGTTTTTGGTTCCAAGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGgt                                                                                                                                            <  2:254311/150‑1 (MQ=255)
          cGCTGTTTTTTTGTTTTTTGTTCCATGTCACTCACTCTTTTTTGAAAATCCATCACT‑‑GGGGGGCTTTTATGGTCTTTGCTTAACCGCAAGGGCGGCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGgtg                                                                                                                                           <  2:258145/150‑1 (MQ=255)
          cGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGgtg                                                                                                                                           <  1:1701154/150‑1 (MQ=255)
          cGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGgtg                                                                                                                                           <  2:53296/150‑1 (MQ=255)
          cGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGgtg                                                                                                                                           <  2:1641770/150‑1 (MQ=255)
           gCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGgtgt                                                                                                                                          <  2:1435425/150‑1 (MQ=255)
            cTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGtt                                                                                                                                         <  1:890429/150‑1 (MQ=255)
            cTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTGTTATCGTCTTTCCTTTACCGCCAGGGCGTCGGCCTCAAGTAGAAAGCAGATAAAAACAAAAACACCCTTGCGCAGGCAATGgtgat                                                                                                                                         >  2:752588/1‑148 (MQ=255)
            cTGTCTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGtt                                                                                                                                         <  2:1677133/150‑1 (MQ=255)
             tGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGttt                                                                                                                                        <  2:353751/150‑1 (MQ=255)
                 tttttgtttttgGTTCCATGTCACTCCCTCTTTTTTGAACATCCATCCCT‑‑GGGGGGCTTTTATCGTATTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGGCGtttttgt                                                                                                                                    <  2:1541019/150‑5 (MQ=255)
                 tttttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGGTGtttttgt                                                                                                                                    <  1:1375353/150‑5 (MQ=255)
                   tttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGGGCAATGGTGtttttgttt                                                                                                                                  <  2:408253/150‑7 (MQ=255)
                     tgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGATTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGACTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCATTGGTGTTTAATCGTCa                                                                                                                                >  2:1123628/1‑150 (MQ=255)
                      gtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCAt                                                                                                                               >  1:1274331/1‑150 (MQ=255)
                      gtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCAt                                                                                                                               >  2:920030/1‑150 (MQ=255)
                      gtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCACAGGCAATGGTGTTTAATCGTCAt                                                                                                                               >  2:480187/1‑150 (MQ=255)
                       tttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCAACCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCGCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCAtt                                                                                                                              >  2:1699251/1‑150 (MQ=255)
                          ttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAg                                                                                                                           <  2:659482/150‑1 (MQ=255)
                           tgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAgg                                                                                                                          <  1:499811/150‑1 (MQ=255)
                           tgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAgg                                                                                                                          <  2:340641/150‑1 (MQ=255)
                             gTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGAc                                                                                                                        <  2:1412839/150‑1 (MQ=255)
                                ccATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGa                                                                                                                     <  1:1313764/150‑1 (MQ=255)
                                 cATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTAATTGAGGACTGAt                                                                                                                    >  2:1032046/1‑150 (MQ=255)
                                  aTGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGCCAATGGTGTTTAATCGTCATTGAGGACTGATg                                                                                                                   >  2:405234/1‑150 (MQ=255)
                                  aTGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGACCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATg                                                                                                                   >  2:1370018/1‑150 (MQ=255)
                                      cactcactCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTa                                                                                                               >  2:1004983/1‑150 (MQ=255)
                                           actcTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTAAATCGTCATTGACGACTGATGGTTATGAAt                                                                                                          >  2:1142742/1‑150 (MQ=255)
                                           actcTTTTTTGAATATCCATCCAT‑‑TGGGGGCTTTTATCGTCTTTGATTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAAAACCATTTCGCATGCTTTGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAAt                                                                                                          <  1:881427/150‑1 (MQ=255)
                                             tctTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTa                                                                                                        >  2:1593604/1‑150 (MQ=255)
                                             tctTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTa                                                                                                        >  1:1341240/1‑150 (MQ=255)
                                              ctTTTTTGAATATCCCTCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCATCGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCAATGCGCAGGCAAAGGTGTTTAATCGACATTGAGGACTGATGGTTATGAATTAc                                                                                                       >  2:1233212/1‑150 (MQ=255)
                                                tttttGAATATCCATCCCTGGGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTAc                                                                                                       <  2:610413/150‑1 (MQ=255)
                                                  tttGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTTCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACtttt                                                                                                   >  1:1667646/1‑150 (MQ=255)
                                                       atatCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCg                                                                                              >  1:1263373/1‑150 (MQ=255)
                                                       atatCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCACAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCg                                                                                              <  2:14421/150‑1 (MQ=255)
                                                        tatCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCgg                                                                                             >  2:1303203/1‑150 (MQ=255)
                                                        tatCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCgg                                                                                             >  2:1588446/1‑150 (MQ=255)
                                                         atccatccCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCggg                                                                                            >  2:1648910/1‑150 (MQ=255)
                                                          tccatccCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCgggg                                                                                           <  1:1296723/150‑1 (MQ=255)
                                                           ccatccCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGc                                                                                          >  2:843456/1‑150 (MQ=255)
                                                             atccCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGGCGGCCGCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCgg                                                                                        >  2:1228827/1‑149 (MQ=255)
                                                                 cT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTc                                                                                    <  1:1004983/150‑1 (MQ=255)
                                                                 cT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTc                                                                                    <  2:1626263/150‑1 (MQ=255)
                                                                  t‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCGAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCt                                                                                   <  2:666891/150‑1 (MQ=255)
                                                                      gggggCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGc                                                                                 <  1:1127614/150‑1 (MQ=255)
                                                                       ggggGTTTTATTCTCTTTTCTTTACCGCCCCGGCGGCGGCCTCAAGTAATAATCATTTAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGcc                                                                                <  2:1659418/150‑1 (MQ=255)
                                                                       ggggCTTTTATCGTCGTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGAAGGCAATGGTGTTTAATCGTCATTGAGTACTGATGGTTATGAATTACTTTTCATCGGGGCGTTTTATGcc                                                                                >  1:1267603/1‑150 (MQ=255)
                                                                       ggggCTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCg                                                                               <  1:1656150/150‑1 (MQ=255)
                                                                            ttttATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAAGAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTg                                                                           <  1:1501707/150‑1 (MQ=255)
                                                                             tttATAGTCTATGCTTTACAGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCATGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCTGGGCGTTTTCTGCCGGTTgg                                                                          <  1:291435/150‑1 (MQ=255)
                                                                              ttATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTggg                                                                         <  1:1062063/150‑1 (MQ=255)
                                                                                    tCTTTGCTTTACCGCCCGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAACACCCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAttt                                                                   <  1:1032874/150‑1 (MQ=255)
                                                                                    tCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAttt                                                                   >  2:850510/1‑150 (MQ=255)
                                                                                     cTTTGCTTTACCGCCAGGGCGTCGGACTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCGGCGGGGCGTTTTCTGCCGGTTGGGTTTTTta                                                                  >  2:97923/1‑150 (MQ=255)
                                                                                      tttGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTCCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTtac                                                                 <  2:185146/150‑1 (MQ=255)
                                                                                          cTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACg                                                             >  2:974541/1‑150 (MQ=255)
                                                                                             tACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTg                                                          >  1:817854/1‑150 (MQ=255)
                                                                                               ccGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGGGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTTTGAATTTCTTTTCAGCGGGGCGTTTTCGGCCGGTTGGGTTATTTACTACGCTGGa                                                        >  1:870293/1‑150 (MQ=255)
                                                                                                cGCCAGGGTGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGAt                                                       <  1:46610/150‑1 (MQ=255)
                                                                                                     gggCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTc                                                  <  1:328399/150‑1 (MQ=255)
                                                                                                           cGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTc                                            <  1:1135796/150‑1 (MQ=255)
                                                                                                             gCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCgg                                          <  1:194268/150‑1 (MQ=255)
                                                                                                              ccTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGt                                         >  1:1417993/1‑150 (MQ=255)
                                                                                                               cTCAAGTATAAAGCAGATAAAAACAAAAACACCATAGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTCATGGTTATCAAATACTTTTCAGCGGGGCGTTTTCTGACTGTTGGGTTATTTACTACGATGGATTTGTCAACTTCGGTa                                        >  1:284214/1‑150 (MQ=255)
                                                                                                                       taAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGTCGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAAATAtttt                                >  2:289374/1‑150 (MQ=255)
                                                                                                                        aaaGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTg                               <  1:998386/150‑1 (MQ=255)
                                                                                                                        aaaGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTg                               >  1:334792/1‑150 (MQ=255)
                                                                                                                         aaGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTgc                              <  2:1097994/150‑1 (MQ=255)
                                                                                                                           gCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCTTTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTgcgc                            >  1:178886/1‑150 (MQ=255)
                                                                                                                            cAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCt                           <  1:1510525/150‑1 (MQ=255)
                                                                                                                             aGATAAAAACAAAAACACCATTGCGAAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCTCTg                          >  1:42545/1‑150 (MQ=255)
                                                                                                                              gATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTgg                         >  1:1570149/1‑150 (MQ=255)
                                                                                                                                 aaaaacaaaaacACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGCCTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTa                      >  2:79926/1‑150 (MQ=255)
                                                                                                                                 aaaaacaaaaacACCATTGCACAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTa                      <  1:480187/150‑1 (MQ=255)
                                                                                                                                  aaaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGTCTGATGGTTATGAATTACTTTTCATCGGGGCGTTTTCTGCCGGTTTGGTTATTTACTACGCTTGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTat                     <  1:451344/150‑2 (MQ=255)
                                                                                                                                  aaaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAg                     <  2:1074998/150‑1 (MQ=255)
                                                                                                                                   aaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGa                    >  2:626156/1‑150 (MQ=255)
                                                                                                                                   aaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGATTTCTGCAGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGa                    >  1:1160949/1‑150 (MQ=255)
                                                                                                                                      caaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACATTCGGTAACTATTTTGCGCTGGTTAGAAAt                 >  1:622847/1‑150 (MQ=255)
                                                                                                                                         aaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAtttt              <  1:815389/150‑1 (MQ=255)
                                                                                                                                          aacaCCATTGCCTGCGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTa             >  2:428351/1‑150 (MQ=255)
                                                                                                                                          aacaCCATTGCCTGCGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTAGTTTGCGCTGGTTAGAAATTTTa             >  1:1160361/1‑150 (MQ=255)
                                                                                                                                          aacaCCATTGCCTACGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTa             >  2:1397317/1‑150 (MQ=255)
                                                                                                                                           acacCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCAGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTAt            >  2:121122/1‑150 (MQ=255)
                                                                                                                                            cacCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATg           <  1:1019703/150‑1 (MQ=255)
                                                                                                                                              ccATTGCGCAGGCAATGGTGTTTAATCGTCAGTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgt         >  2:206832/1‑150 (MQ=255)
                                                                                                                                               cATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtc        >  2:571563/1‑150 (MQ=255)
                                                                                                                                                aTTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtcc       <  1:29213/150‑1 (MQ=255)
                                                                                                                                                    cgcAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGATGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTAAGAAATTTTATGgtccattc   >  2:1077463/1‑150 (MQ=255)
                                                                                                                                                     gcAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtccagtcc  <  2:911026/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
ATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCT‑‑GGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCC  >  NC_000913/1555634‑1555930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: