BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	144,806	C→A	6.1%	S77* (TCA→TAA)	yadI →	putative PTS Enzyme IIA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	144,806	0	C	A	6.1%	341.9 / 8.4	115	S77* (TCA→TAA)	yadI	putative PTS Enzyme IIA
Reads supporting (aligned to +/- strand): ref base C (37/71); new base A (5/2); total (42/73)
Fisher's exact test for biased strand distribution p-value = 9.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

Only 100 of 117 total aligned reads displayed.

TCTTCAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTC  >  NC_000913/144657‑144955

   tcAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCatt                                                                                                                                                    <  2:738908/150‑1 (MQ=255)

   tcAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTAata                                                                                                                                                    >  2:664148/1‑149 (MQ=255)

    cAGTGCCGGGCCGTCAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGGGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCatta                                                                                                                                                   <  1:953360/150‑1 (MQ=255)

      gTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCattatt                                                                                                                                                 >  2:1049280/1‑150 (MQ=255)

          cGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGc                                                                                                                                             >  2:811227/1‑150 (MQ=255)

           gggCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTAATAATGAAGcc                                                                                                                                            <  1:664148/150‑1 (MQ=255)

             gCCGAGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACAGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCcac                                                                                                                                          >  1:1406800/1‑150 (MQ=255)

               cGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTAATAATGAAGCcacgc                                                                                                                                        <  1:669806/150‑3 (MQ=255)

                 tGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAac                                                                                                                                      <  1:459198/150‑1 (MQ=255)

                  gAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAaca                                                                                                                                     >  2:365499/1‑150 (MQ=255)

                      ttCTGGCGCGGATTAAGCGCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTcc                                                                                                                                 <  1:1194368/150‑1 (MQ=255)

                       tCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTccc                                                                                                                                <  2:1262130/150‑1 (MQ=255)

                               ggATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGAGaa                                                                                                                        >  2:1445048/1‑150 (MQ=255)

                               ggATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATAGCGTGTCTTCATTATTAAGCAACAAACACTCCCGTTGCGaa                                                                                                                        >  2:672938/1‑150 (MQ=255)

                                    aaGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGAt                                                                                                                   <  1:1480479/150‑1 (MQ=255)

                                     aGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGAtt                                                                                                                  >  1:1335898/1‑150 (MQ=255)

                                      gCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGAttt                                                                                                                 >  1:91102/1‑150 (MQ=255)

                                         ctAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTg                                                                                                              <  1:1441264/150‑1 (MQ=255)

                                         ctAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCAGCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTg                                                                                                              <  2:1286267/150‑1 (MQ=255)

                                          tAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTgg                                                                                                             <  1:1606900/150‑1 (MQ=255)

                                             tatGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGTGGAATCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGgtgt                                                                                                          <  2:1061043/150‑1 (MQ=255)

                                             tatGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGgtgt                                                                                                          >  1:827429/1‑150 (MQ=255)

                                               tGCTCAGCCGCATGTTGTGCGATGCTCTGCATGTAGCGGACTCTGGTGAGGGTGTCATCTTCTTTACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCa                                                                                                        <  1:302730/150‑1 (MQ=255)

                                               tGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCa                                                                                                        >  2:928999/1‑150 (MQ=255)

                                                 cgcAGCCGCATGATGGGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACg                                                                                                      <  1:406238/148‑1 (MQ=255)

                                                     gCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTAc                                                                                                  >  2:6836/1‑150 (MQ=255)

                                                     gCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTAc                                                                                                  <  1:1062333/150‑1 (MQ=255)

                                                      ccGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTAcc                                                                                                 <  1:1257335/150‑1 (MQ=255)

                                                       cGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCg                                                                                                <  2:1353296/150‑1 (MQ=255)

                                                       cGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCg                                                                                                <  2:299425/150‑1 (MQ=255)

                                                        gCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACACCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGt                                                                                               >  2:357738/1‑150 (MQ=255)

                                                         cATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGtt                                                                                              <  2:39682/150‑1 (MQ=255)

                                                         cATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGtt                                                                                              <  2:971689/150‑1 (MQ=255)

                                                           tgatgTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTaa                                                                                            >  2:1086991/1‑150 (MQ=255)

                                                            gatgTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGTGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAAt                                                                                           <  2:709506/150‑1 (MQ=255)

                                                               gtgCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGa                                                                                        <  1:331531/150‑1 (MQ=255)

                                                                 gCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAAc                                                                                      <  1:274707/150‑1 (MQ=255)

                                                                   gATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAg                                                                                    <  2:1592247/150‑1 (MQ=255)

                                                                    aTGCTCTGCATGTAGCTGAGTCTGGTTAGGCTGTCATCTTCTTAACCGATATAGACGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCAACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAga                                                                                   <  1:258210/150‑1 (MQ=255)

                                                                     tGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAgat                                                                                  <  1:1707009/150‑1 (MQ=255)

                                                                        tctGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCCCCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAgatgat                                                                               >  1:1225239/1‑150 (MQ=255)

                                                                         cttcATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATATCCGGAGCGCCACCGTTTCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGatgatg                                                                              <  1:311078/147‑1 (MQ=255)

                                                                            cATGAAGTGGACTCTGGTGAGGGTGTTATCTTCTTTAGCGATATAGCCGGAGCGCATACGTATAGTGTTGCTTCATTTTTAAGCTACAAACACTCCCGTTGCGTAGTGATTTCTGGTGTCACGTTAACGTTAATTGAACAGATGATGGCt                                                                           <  2:1116976/150‑1 (MQ=255)

                                                                              tGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCTGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTg                                                                         <  2:669528/150‑1 (MQ=255)

                                                                               gAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGc                                                                        <  1:457796/150‑1 (MQ=255)

                                                                               gAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGc                                                                        <  1:1049280/150‑1 (MQ=255)

                                                                               gAAGCGGACTCTCGTGAGGGTGTCATCTTCTAAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATAGAACAGATGATGTCTTGc                                                                        >  2:1528052/1‑150 (MQ=255)

                                                                                aaGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGcc                                                                       <  2:1212622/150‑1 (MQ=255)

                                                                                 aGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCg                                                                      <  1:1664254/150‑1 (MQ=255)

                                                                                 aGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCg                                                                      <  1:1657642/150‑1 (MQ=255)

                                                                                 aGCGGACTCTGGTGAGGGTCGCATCTTCTTAAACGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACTCTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCg                                                                      <  1:263088/150‑1 (MQ=255)

                                                                                       ctctGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAAc                                                                >  2:790954/1‑150 (MQ=255)

                                                                                        tctGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAAcc                                                               <  2:1340526/150‑1 (MQ=255)

                                                                                         cttgTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTCTCGCGGGGCTTCATTATTACGCCACAAACACTCCCTTTTCGAATTGATTTCTGGCGTCACGCTACCGTTAATGGAACAGATGCGGGCCTGCCGTGAAACCa                                                              <  2:289298/147‑1 (MQ=255)

                                                                                         ctGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCa                                                              <  2:1694635/150‑1 (MQ=255)

                                                                                          tGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCAt                                                             <  1:1586440/150‑1 (MQ=255)

                                                                                           ggTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATg                                                            <  2:749300/150‑1 (MQ=255)

                                                                                                   tgtCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTc                                                    <  2:871658/150‑1 (MQ=255)

                                                                                                     tCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACGATTTCat                                                  >  1:1075565/1‑149 (MQ=255)

                                                                                                     gcATCTTCTTAACCGATCTAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGGGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCag                                                  <  2:297091/149‑1 (MQ=255)

                                                                                                       aTCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCagag                                                <  1:1098556/150‑1 (MQ=255)

                                                                                                       aTCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCagag                                                <  1:1545428/150‑1 (MQ=255)

                                                                                                        tcttctTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTGGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGt                                               <  2:91102/150‑1 (MQ=255)

                                                                                                         cttcttAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGtt                                              <  1:176460/150‑1 (MQ=255)

                                                                                                           tcttAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTc                                            >  2:627288/1‑150 (MQ=255)

                                                                                                           tcttAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTc                                            <  2:1518901/150‑1 (MQ=255)

                                                                                                             ttAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTcgc                                          <  1:709965/150‑1 (MQ=255)

                                                                                                              tAACCGATATAGCCGGATCGCCACCGTATCGCGTGGCTGCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGGCACGTTACCGTTAATTGAACAGATGATGGCGTGCCGTGAAACCATGACCAGTTCAGAGTTTcgcg                                         <  1:1047881/150‑1 (MQ=255)

                                                                                                              tAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCATCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTcgcg                                         >  2:724734/1‑150 (MQ=255)

                                                                                                                aCCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAACCCATGACCAGTTCAGAGTTTCGCGAg                                       >  2:233813/1‑150 (MQ=255)

                                                                                                                aCCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAg                                       <  2:1472178/150‑1 (MQ=255)

                                                                                                                aCCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAg                                       <  2:1186169/150‑1 (MQ=255)

                                                                                                                 ccGATATAGCCGGAGCGACACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATAGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGc                                      >  2:1724038/1‑150 (MQ=255)

                                                                                                                    atatAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTa                                   <  1:811227/150‑1 (MQ=255)

                                                                                                                     tataGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTAt                                  >  1:476249/1‑150 (MQ=255)

                                                                                                                     tataGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTAt                                  <  2:569659/150‑1 (MQ=255)

                                                                                                                       taGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTg                                >  2:700464/1‑150 (MQ=255)

                                                                                                                        aGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGt                               >  2:1686494/1‑150 (MQ=255)

                                                                                                                        aGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGt                               >  1:1437241/1‑150 (MQ=255)

                                                                                                                         gCCGGAGCGCCACCGTATCGCTTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTc                              <  2:1524237/150‑1 (MQ=255)

                                                                                                                          ccGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCg                             <  1:998822/150‑1 (MQ=255)

                                                                                                                          ccGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGAGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCg                             >  2:792295/1‑150 (MQ=255)

                                                                                                                           cGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTTCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGa                            <  1:1329265/150‑1 (MQ=255)

                                                                                                                                cgcCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGATCAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTgg                       >  2:166502/1‑150 (MQ=255)

                                                                                                                                   cACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTg                    <  1:1086991/150‑1 (MQ=255)

                                                                                                                                    aCCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCTAGCGTATTGTCGAACTGGGTgc                   >  2:1428756/1‑150 (MQ=255)

                                                                                                                                    aCCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTgc                   <  1:159394/150‑1 (MQ=255)

                                                                                                                                       gTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGcc                <  1:700464/150‑1 (MQ=255)

                                                                                                                                         aTCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCAGGTGTCACGTTACCGTTAATTGAACAGATGATGCCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCTAGCGTATTGTCGAACAGGGTGCGCCgg              >  1:1274660/1‑150 (MQ=255)

                                                                                                                                          tCGCGTGGCTTCATTATTAAG‑CACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAg            <  2:959196/150‑1 (MQ=255)

                                                                                                                                          gtttgTGGCTTAATAATGAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAACCCAAGACCAGTTCAGACTTTCGCGAGCGTATTGTCGAACAGGGTGCGCCGGa             >  2:786260/5‑150 (MQ=255)

                                                                                                                                           tttgTGGCTTAATAATGAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAg            >  1:968515/4‑150 (MQ=255)

                                                                                                                                            gcgTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAgg           >  1:144174/1‑150 (MQ=255)

                                                                                                                                            gcgTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAgg           >  2:1465540/1‑150 (MQ=255)

                                                                                                                                             tgTGGCTTAATAATGAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGt          >  1:399691/2‑150 (MQ=255)

                                                                                                                                              gTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTg         <  1:365499/150‑1 (MQ=255)

                                                                                                                                               gggCTTCATAATTAAGCCAAAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGa        <  1:760609/149‑1 (MQ=255)

                                                                                                                                                  cTTCAATATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTAACGTTAATTGAACAGATGATGGATTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCTAGCGTATTGTCGAACAGGGTGCGCCGGAGGTCagta     >  1:47958/1‑150 (MQ=255)

                                                                                                                                                   ttCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGagtag    <  1:851093/150‑1 (MQ=255)

                                                                                                                                                    tCATTATTAAGCCACAAACACTCCCGTTTCGAACACATAACTGGAGTCACGTTACCGTTAATTGAAAAGATGATCGCTAGCCGTGAAACCATGACCAGTTCAGAGTATCGCGAGCCAATTGTCGAACTGGGTGCGCCGGAGGTGagtagt   >  2:1239997/1‑150 (MQ=255)

TCTTCAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTC  >  NC_000913/144657‑144955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`