Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,555,783 A→G 5.9% intergenic (‑114/+43) maeA ← / ← sra malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme/stationary‑phase‑induced ribosome‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,555,7830AG 5.9% 407.9 / 11.5 137intergenic (‑114/+43)maeA/sramalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme/stationary‑phase‑induced ribosome‑associated protein
Reads supporting (aligned to +/- strand):  ref base A (67/61);  new base G (3/5);  total (70/67)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.43e-01

Only 100 of 139 total aligned reads displayed.

ATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAA  >  NC_000913/1555634‑1555932
                                                                                                                                                     |                                                                                                                                                     
ataAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTATGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCa                                                                                                                                                       >  1:1720192/1‑150 (MQ=255)
 taAAGCGAACGCTGTTTTTTTGTTTTTGTTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGTGTGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAg                                                                                                                                                      <  1:322711/150‑1 (MQ=255)
 taAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAg                                                                                                                                                      >  2:815389/1‑150 (MQ=255)
 taAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAg                                                                                                                                                      >  2:1174371/1‑150 (MQ=255)
 taAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAg                                                                                                                                                      >  2:1019703/1‑150 (MQ=255)
  aaaGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATACATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAATCAGATAAAAACAAAAACACCATTGCGCAgg                                                                                                                                                     >  2:1205973/1‑150 (MQ=255)
    aGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCa                                                                                                                                                   >  2:862568/1‑150 (MQ=255)
        aaCGCTGTTTTTTTGGTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCCTCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCACGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATgg                                                                                                                                               <  2:1000452/150‑1 (MQ=255)
         aCGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGgt                                                                                                                                              <  2:931163/150‑1 (MQ=255)
         aCGCTGTTTTTTTGTTTTTGGTTCCAAGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGgt                                                                                                                                              <  2:254311/150‑1 (MQ=255)
           gCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGgtgt                                                                                                                                            <  2:1435425/150‑1 (MQ=255)
            cTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGtt                                                                                                                                           <  2:1146526/150‑1 (MQ=255)
            cTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTGTTATCGTCTTTCCTTTACCGCCAGGGCGTCGGCCTCAAGTAGAAAGCAGATAAAAACAAAAACACCCTTGCGCAGGCAATGgtgat                                                                                                                                           >  2:752588/1‑148 (MQ=255)
             tGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGttt                                                                                                                                          <  2:353751/150‑1 (MQ=255)
                 tttttgtttttgGTTCCATGTCACTCCCTCTTTTTTGAACATCCATCCCTGGGGGGCTTTTATCGTATTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGGCGtttttgt                                                                                                                                      <  2:1541019/150‑5 (MQ=255)
                 tttttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGGTGtttttgt                                                                                                                                      <  1:1375353/150‑5 (MQ=255)
                  ttttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCg                                                                                                                                     <  2:1123601/150‑1 (MQ=255)
                     tgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGATTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGACTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCATTGGTGTTTAATCGTCa                                                                                                                                  >  2:1123628/1‑150 (MQ=255)
                      gtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCAt                                                                                                                                 >  1:1274331/1‑150 (MQ=255)
                      gtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCAt                                                                                                                                 >  2:920030/1‑150 (MQ=255)
                      gtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCACAGGCAATGGTGTTTAATCGTCAt                                                                                                                                 >  2:480187/1‑150 (MQ=255)
                       tttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCAACCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCGCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCAtt                                                                                                                                >  2:1699251/1‑150 (MQ=255)
                          ttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCCGGGCGGCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAg                                                                                                                             <  2:659481/150‑1 (MQ=255)
                          ttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAg                                                                                                                             <  2:659482/150‑1 (MQ=255)
                           tgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAgg                                                                                                                            <  1:1605861/150‑1 (MQ=255)
                           tgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAgg                                                                                                                            <  1:499811/150‑1 (MQ=255)
                             gTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGAc                                                                                                                          <  2:1412839/150‑1 (MQ=255)
                                ccATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGa                                                                                                                       <  1:1313764/150‑1 (MQ=255)
                                 cATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGAt                                                                                                                      >  2:1436952/1‑150 (MQ=255)
                                  aTGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGCCAATGGTGTTTAATCGTCATTGAGGACTGATg                                                                                                                     >  2:405234/1‑150 (MQ=255)
                                  aTGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGACCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATg                                                                                                                     >  2:1370018/1‑150 (MQ=255)
                                     tctctcactcTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGtt                                                                                                                  >  2:29213/4‑150 (MQ=255)
                                      cactcactCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTa                                                                                                                 >  1:911026/1‑150 (MQ=255)
                                         acattattttttGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGa                                                                                                              >  2:474893/7‑150 (MQ=255)
                                           actcTTTTTTGAATATCCATCCATTGGGGGCTTTTATCGTCTTTGATTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAAAACCATTTCGCATGCTTTGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAAt                                                                                                            <  1:881427/150‑1 (MQ=255)
                                               ttttttGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCTTGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACt                                                                                                        <  2:1565871/150‑1 (MQ=255)
                                                  tttGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTTCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACtttt                                                                                                     >  1:1667646/1‑150 (MQ=255)
                                                       atatCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCg                                                                                                >  1:1263373/1‑150 (MQ=255)
                                                       atatCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCACAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCg                                                                                                <  2:14421/150‑1 (MQ=255)
                                                        tatCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCgg                                                                                               >  2:1588446/1‑150 (MQ=255)
                                                        tatCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCgg                                                                                               >  2:1303203/1‑150 (MQ=255)
                                                         atccatccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCggg                                                                                              >  2:1648910/1‑150 (MQ=255)
                                                          tccatccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCgggg                                                                                             <  1:1296723/150‑1 (MQ=255)
                                                           ccatccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGc                                                                                            >  2:843456/1‑150 (MQ=255)
                                                            catccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGAATGATGGTTATGAATTACTTTTCAGCGGTGCg                                                                                           >  2:1331144/1‑150 (MQ=255)
                                                             atccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGGCGGCCGCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCgg                                                                                          >  2:1228827/1‑149 (MQ=255)
                                                              tccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGtt                                                                                         <  2:1605393/150‑1 (MQ=255)
                                                               cccTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGttt                                                                                        <  1:850686/150‑1 (MQ=255)
                                                                ccTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGtttt                                                                                       <  2:1659272/150‑1 (MQ=255)
                                                                 cTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTc                                                                                      <  1:1004983/150‑1 (MQ=255)
                                                                 cTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTc                                                                                      <  2:1626263/150‑1 (MQ=255)
                                                                  tGGGGGGCTTTTATCGTCTTTGCTTTACCGCGAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCt                                                                                     <  2:666891/150‑1 (MQ=255)
                                                                  tGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCt                                                                                     <  2:240817/150‑1 (MQ=255)
                                                                    gggggCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGc                                                                                   <  1:1127614/150‑1 (MQ=255)
                                                                     ggggGTTTTATTCTCTTTTCTTTACCGCCCCGGCGGCGGCCTCAAGTAATAATCATTTAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGcc                                                                                  <  2:1659418/150‑1 (MQ=255)
                                                                     ggggCTTTTATCGTCGTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGAAGGCAATGGTGTTTAATCGTCATTGAGTACTGATGGTTATGAATTACTTTTCATCGGGGCGTTTTATGcc                                                                                  >  1:1267603/1‑150 (MQ=255)
                                                                     ggggCTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCg                                                                                 <  1:1656150/150‑1 (MQ=255)
                                                                         cTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAATCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCAGGtt                                                                              >  1:698255/1‑150 (MQ=255)
                                                                         cTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTg                                                                                    <  2:1037433/144‑1 (MQ=255)
                                                                          ttttATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAAGAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTg                                                                             <  1:1501707/150‑1 (MQ=255)
                                                                           tttATAGTCTATGCTTTACAGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCATGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCTGGGCGTTTTCTGCCGGTTgg                                                                            <  1:291435/150‑1 (MQ=255)
                                                                            ttATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTggg                                                                           <  1:1062063/150‑1 (MQ=255)
                                                                             tATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGt                                                                          <  1:474893/150‑1 (MQ=255)
                                                                                  tCTTTGCTTTACCGCCCGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAACACCCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAttt                                                                     <  1:1032874/150‑1 (MQ=255)
                                                                                  tCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAttt                                                                     >  2:850510/1‑150 (MQ=255)
                                                                                   cTTTGCTTTACCGCCAGGGCGTCGGACTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCGGCGGGGCGTTTTCTGCCGGTTGGGTTTTTta                                                                    >  2:97923/1‑150 (MQ=255)
                                                                                        cTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACg                                                               >  2:974541/1‑150 (MQ=255)
                                                                                             ccGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGGGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTTTGAATTTCTTTTCAGCGGGGCGTTTTCGGCCGGTTGGGTTATTTACTACGCTGGa                                                          >  1:870293/1‑150 (MQ=255)
                                                                                              cGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGAt                                                         <  2:1720192/150‑1 (MQ=255)
                                                                                                   gggCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTc                                                    <  1:328399/150‑1 (MQ=255)
                                                                                                         cGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTc                                              <  1:1135796/150‑1 (MQ=255)
                                                                                                           gCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCgg                                            <  1:194268/150‑1 (MQ=255)
                                                                                                            ccTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGt                                           >  1:1417993/1‑150 (MQ=255)
                                                                                                             cTCAAGTATAAAGCAGATAAAAACAAAAACACCATAGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTCATGGTTATCAAATACTTTTCAGCGGGGCGTTTTCTGACTGTTGGGTTATTTACTACGATGGATTTGTCAACTTCGGTa                                          >  1:284214/1‑150 (MQ=255)
                                                                                                                     taAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGTCGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAAATAtttt                                  >  2:289374/1‑150 (MQ=255)
                                                                                                                      aaaGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTg                                 <  1:998386/150‑1 (MQ=255)
                                                                                                                      aaaGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTg                                 >  1:334792/1‑150 (MQ=255)
                                                                                                                         gCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCTTTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTgcgc                              >  1:178886/1‑150 (MQ=255)
                                                                                                                          cAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCt                             <  1:1510525/150‑1 (MQ=255)
                                                                                                                           aGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTg                            >  2:363080/1‑150 (MQ=255)
                                                                                                                           aGATAAAAACAAAAACACCATTGCGAAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCTCTg                            >  1:42545/1‑150 (MQ=255)
                                                                                                                               aaaaacaaaaacACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGCCTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTa                        >  2:79926/1‑150 (MQ=255)
                                                                                                                               aaaaacaaaaacACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGTGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTa                        <  2:1226251/150‑1 (MQ=255)
                                                                                                                               aaaaacaaaaacACCATTGCACAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTa                        <  1:480187/150‑1 (MQ=255)
                                                                                                                                aaaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGTCTGATGGTTATGAATTACTTTTCATCGGGGCGTTTTCTGCCGGTTTGGTTATTTACTACGCTTGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTat                       <  1:451344/150‑2 (MQ=255)
                                                                                                                                aaaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAg                       <  2:1074998/150‑1 (MQ=255)
                                                                                                                                 aaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGa                      >  2:626156/1‑150 (MQ=255)
                                                                                                                                 aaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGATTTCTGCAGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGa                      >  1:1160949/1‑150 (MQ=255)
                                                                                                                                  aacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGaa                     >  2:1399993/1‑150 (MQ=255)
                                                                                                                                  aacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTCATGGTTATGAATTACATTTCAGCGGGGAGTTTTCTGCCGGTTGGGTTATTTACTACGCTTTATTTGTCACCTTCGGTAACTATTTTGCGATGGTTAGaa                     >  2:1016169/1‑150 (MQ=255)
                                                                                                                                       aaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAtttt                <  1:815389/150‑1 (MQ=255)
                                                                                                                                        aacaCCATTGCCTGCGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTa               >  2:603027/1‑150 (MQ=255)
                                                                                                                                        aacaCCATTGCCTGCGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTa               >  2:428351/1‑150 (MQ=255)
                                                                                                                                        aacaCCATTGCCTGCGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTAGTTTGCGCTGGTTAGAAATTTTa               >  1:1160361/1‑150 (MQ=255)
                                                                                                                                          cacCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATg             <  1:1019703/150‑1 (MQ=255)
                                                                                                                                            ccATTGCGCAGGCAATGGTGTTTAATCGTCAGTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgt           >  2:206832/1‑150 (MQ=255)
                                                                                                                                             cATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtc          >  2:571563/1‑150 (MQ=255)
                                                                                                                                              aTTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtcc         <  1:1370018/150‑1 (MQ=255)
                                                                                                                                                  cgcAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGATGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTAAGAAATTTTATGgtccattc     >  2:1077463/1‑150 (MQ=255)
                                                                                                                                                   gcAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtccagtcc    <  2:911026/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAA  >  NC_000913/1555634‑1555932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: