Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,567,471 A→C 12.3% intergenic (‑331/+33) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,567,4710AC12.3% 290.9 / 30.3 121intergenic (‑331/+33)dosC/yddWdiguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
Reads supporting (aligned to +/- strand):  ref base A (50/56);  new base C (4/11);  total (54/67)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

Only 100 of 122 total aligned reads displayed.

GGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGAT  >  NC_000913/1567327‑1567619
                                                                                                                                                |                                                                                                                                                    
ggTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCAcc                                                                                                                                                 >  2:1069341/1‑150 (MQ=255)
ggTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCAcc                                                                                                                                                 >  2:747963/1‑150 (MQ=255)
    aCAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCt                                                                                                                                             <  1:1001034/150‑1 (MQ=255)
    aCAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCt                                                                                                                                             <  2:1694235/150‑1 (MQ=255)
    aCAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCt                                                                                                                                             <  1:152264/150‑1 (MQ=255)
    aCAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTAACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCt                                                                                                                                             <  1:1422283/150‑1 (MQ=255)
    aCAGCGAATATCGTATGGTTTTTCATATTCATACATTTTAATTATGGATTTATGGCTGTTTAACTAAGTTTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAATCAGGTGCTTTACCCACCGGCt                                                                                                                                             <  2:1118922/150‑1 (MQ=255)
    aCAGAGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGTGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCt                                                                                                                                             <  1:48596/150‑1 (MQ=255)
     cAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCtt                                                                                                                                            <  2:1056295/150‑1 (MQ=255)
      ggCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCTTg                                                                                                                                           <  2:1619519/149‑1 (MQ=255)
      cgCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCTTg                                                                                                                                           <  2:1625994/149‑1 (MQ=255)
      aGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTg                                                                                                                                           <  1:414461/150‑1 (MQ=255)
      aGCGAATATCGTATGGTTTTTCATATTAATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTg                                                                                                                                           >  1:1427646/1‑150 (MQ=255)
        cGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGtt                                                                                                                                         <  2:1277144/150‑1 (MQ=255)
        cGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGtt                                                                                                                                         <  1:730715/150‑1 (MQ=255)
        cGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGtt                                                                                                                                         <  1:44676/150‑1 (MQ=255)
              tCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAg                                                                                                                                   <  2:333502/150‑1 (MQ=255)
               cGTATGGTTTTTCATATTCATACTTTTTTATTAGGGATTTATGGCTGTTAAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGt                                                                                                                                  <  2:295361/150‑1 (MQ=255)
               cGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGt                                                                                                                                  >  2:1265632/1‑150 (MQ=255)
               cGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGt                                                                                                                                  >  1:1724649/1‑150 (MQ=255)
               cGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGt                                                                                                                                  <  1:131181/150‑1 (MQ=255)
                gTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTa                                                                                                                                 <  2:781015/150‑1 (MQ=255)
                 tATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTaa                                                                                                                                >  1:740495/1‑150 (MQ=255)
                  aTGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAg                                                                                                                               >  2:295954/1‑150 (MQ=255)
                     gTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGATTGTTACAAAGTAAGAAt                                                                                                                            >  1:1184776/1‑150 (MQ=255)
                       ttttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTTAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATgg                                                                                                                          <  1:146620/150‑1 (MQ=255)
                       ttttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATgg                                                                                                                          <  1:1306793/150‑1 (MQ=255)
                       ttttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATgg                                                                                                                          <  1:360976/150‑1 (MQ=255)
                        tttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATggg                                                                                                                         <  2:230758/150‑1 (MQ=255)
                        tttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATggg                                                                                                                         >  1:778559/1‑150 (MQ=255)
                           catattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGt                                                                                                                      >  2:926322/1‑150 (MQ=255)
                           catattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGt                                                                                                                      >  2:1211807/1‑150 (MQ=255)
                           catattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGt                                                                                                                      >  1:656894/1‑150 (MQ=255)
                           aatattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGt                                                                                                                      <  1:50172/149‑1 (MQ=255)
                             ttttcataCATTTTTATTAGGGATTTATTGATGTTTAACTAAGGGGGGGTAATTTGACTTAAGTAAGACTTATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGttt                                                                                                                    <  2:853202/148‑1 (MQ=255)
                             tattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGttt                                                                                                                    <  2:596968/150‑1 (MQ=255)
                                    aCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGcc                                                                                                             <  2:448686/150‑1 (MQ=255)
                                    aCAATTTAAATAGGGATTTATGGCTGTTTAACTACGTCTGGCTAATTTGACTTAAGTCAGCATGATTATAAGTCGGATAGATTAAGAGGGTAACAAGCCGGTGGGTAACGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGcc                                                                                                             <  2:448690/150‑1 (MQ=255)
                                                gggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATg                                                                                                                           <  1:672765/124‑1 (MQ=255)
                                                gggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATg                                                                                                                           >  2:672765/1‑124 (MQ=255)
                                                  gATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCa                                                                                               <  2:542067/150‑1 (MQ=255)
                                                  gATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCa                                                                                               <  1:1065035/150‑1 (MQ=255)
                                                   ctttATGGCTGTTAAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTACTCGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCGTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAg                                                                                              <  2:273886/149‑1 (MQ=255)
                                                     ttATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGAt                                                                                            <  2:1724649/150‑1 (MQ=255)
                                                        tGGCTGCTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGc                                                                                         <  2:1534350/150‑1 (MQ=255)
                                                         ggCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCt                                                                                        <  2:545600/150‑1 (MQ=255)
                                                             gTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGAACCGGATTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCCACTTTGAAGATAGCTGAcc                                                                                    >  2:744840/1‑150 (MQ=255)
                                                                tAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCt                                                                                 >  1:900761/1‑150 (MQ=255)
                                                                  aCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttg                                                                               >  1:389069/1‑150 (MQ=255)
                                                                  aCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGAATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTAGTTACAAAGTAAGAAAGGGAGTTTAAATGCCCCAGCGACTTTGCACATAGCTGACCGAttg                                                                               >  2:68476/1‑150 (MQ=255)
                                                                     acgagaggTTAATTTGACTTACGTAAGCCTGATTATTACTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGCTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgttg                                                                            <  2:137542/144‑1 (MQ=255)
                                                                     aaGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgttg                                                                            <  2:162148/150‑1 (MQ=255)
                                                                      aGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGa                                                                           >  2:1027029/1‑150 (MQ=255)
                                                                       ttgtgGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAg                                                                          <  2:616939/149‑1 (MQ=255)
                                                                       gtgtGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAg                                                                          <  2:468912/150‑1 (MQ=255)
                                                                         gtgGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTc                                                                        <  1:713822/150‑1 (MQ=255)
                                                                           ggTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACttt                                                                                                  >  2:528213/1‑122 (MQ=255)
                                                                           ggTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACttt                                                                                                  <  1:528213/122‑1 (MQ=255)
                                                                           ggTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTg                                                                      >  1:643725/1‑150 (MQ=255)
                                                                            gTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATTGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGc                                                                     >  1:164905/1‑150 (MQ=255)
                                                                            gTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGCAGTTTAACTGCCCAAGCGAATTTGCATATAGCTTACCAATTGTTGAGTCTga                                                                     >  2:725508/1‑149 (MQ=255)
                                                                              tAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCgg                                                                   <  1:840398/150‑1 (MQ=255)
                                                                                aTTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGtt                                                                 <  1:1489490/150‑1 (MQ=255)
                                                                                aTTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGtt                                                                 >  1:1362592/1‑150 (MQ=255)
                                                                                  ttGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGCCTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTa                                                               >  2:1210307/1‑150 (MQ=255)
                                                                                  ttGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGtttt                                                               >  2:448780/1‑149 (MQ=255)
                                                                                   tGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTAt                                                              >  1:1292051/1‑150 (MQ=255)
                                                                                   tGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTAt                                                              >  2:1721488/1‑150 (MQ=255)
                                                                                     aCTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTc                                                            <  2:740495/150‑1 (MQ=255)
                                                                                      cTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTAttaa                                                           >  2:54255/1‑148 (MQ=255)
                                                                                        taagtaagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGa                                                         <  1:926322/150‑1 (MQ=255)
                                                                                        taagtaagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGa                                                         >  1:1119328/1‑150 (MQ=255)
                                                                                               gCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCt                                                  <  1:440125/150‑1 (MQ=255)
                                                                                                 aTGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGATTATAACTGCCAAAGCGACTTTGCAGATAGCTGAACGCGTGATGAGTCTGCGGTTTATTCAGATAGtcatca                                                >  1:239097/1‑150 (MQ=255)
                                                                                                  tGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCTGTCGGTAAAGCACCGGCTTGTTACAAAGAAAGAATGTGAGATTAACTGCCCCAGCTACTTTGCTGATAGCTGACAGCTTGTTGAGTCTCCGGTTGAATCACTTAGTCCTCAc                                               >  1:289099/1‑150 (MQ=255)
                                                                                                  tGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCAc                                               >  2:935879/1‑150 (MQ=255)
                                                                                                   gATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACg                                              <  1:1608684/150‑1 (MQ=255)
                                                                                                   gATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACg                                              <  1:119570/150‑1 (MQ=255)
                                                                                                        ttaGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACa                                         <  2:1654345/150‑1 (MQ=255)
                                                                                                             gggATAGTTTAAGAGGGTAACAAGCCGGTGTGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATg                                    <  1:1175546/150‑1 (MQ=255)
                                                                                                             gggATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATg                                    >  2:1017755/1‑150 (MQ=255)
                                                                                                              ggATACTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTACGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATgg                                   <  2:1488682/150‑1 (MQ=255)
                                                                                                                        agagGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAAt                         >  2:1168609/1‑150 (MQ=255)
                                                                                                                            ggTAACACGCCGGTGGTTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACTGAACAAGATGGTGCCGCTAATTTCg                     <  1:1069341/150‑1 (MQ=255)
                                                                                                                            ggTAACAAGCCGGTGTGTAAAGCACCGGCTTGTTACATAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCTGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCg                     <  1:522615/150‑1 (MQ=255)
                                                                                                                            ggTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCg                     <  1:747963/150‑1 (MQ=255)
                                                                                                                              tAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCggg                   >  2:26303/1‑150 (MQ=255)
                                                                                                                              tAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCggg                   >  2:626827/1‑150 (MQ=255)
                                                                                                                              tAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGAACGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCCAAtttgctt                   >  2:1526145/1‑146 (MQ=255)
                                                                                                                               aaCAAGCCTGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGATTGTTGATTCTGCGGTTTATTCAGATAGTACTCACGGAAAAAGATGGTGCAGCTAATTTCGGGc                  >  2:1349327/1‑150 (MQ=255)
                                                                                                                               aaCAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGc                  <  1:1027029/150‑1 (MQ=255)
                                                                                                                                aCAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGCCTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGca                 >  1:1197848/1‑150 (MQ=255)
                                                                                                                                aCAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTAt                                                              <  2:125715/105‑1 (MQ=38)
                                                                                                                                aCAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTAt                                                              >  1:125715/1‑105 (MQ=38)
                                                                                                                                aCAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGca                 >  2:1366681/1‑150 (MQ=255)
                                                                                                                                   aGCCGGCGGGCAAAGCACCGGCTTGTTACAAAGTAACAATCCGCCTCAAACCGCCCCCGCGACCTTCCAGATAGCTGACCGCCTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGCAACAAGCTGGCGCCGCTAATTTCGGGCACAg              <  2:847251/150‑1 (MQ=255)
                                                                                                                                         tGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGt        >  2:1351292/1‑150 (MQ=255)
                                                                                                                                          tggTAAAGCACCGGCTTGTTAGAAAGAAAGACTTGGACTCAACCTGACCCAGCGACCTTGCAGATAGCTGACCGCGGGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGTAACAACATGGTGCCGCTAATTTCGGGCACATCATCGtt       <  2:295446/149‑1 (MQ=255)
                                                                                                                                            gTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTaa     >  1:1706480/1‑150 (MQ=255)
                                                                                                                                               aaGCACCGGCTTGTTACAAAGAAACACTGGCCGAGACACTCCCCCAGCGACTATGCCGCTGGCTGACCGCTTGTTGACTCTTCGGATTATTCAGATAGTCCTCACGTAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGAt  <  2:1538713/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGAT  >  NC_000913/1567327‑1567619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: