BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	144,809	T→A	5.8%	L78* (TTA→TAA)	yadI →	putative PTS Enzyme IIA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	144,809	0	T	A	5.8%	283.4 / 4.6	110	L78* (TTA→TAA)	yadI	putative PTS Enzyme IIA
Reads supporting (aligned to +/- strand): ref base T (37/66); new base A (4/3); total (41/69)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

Only 100 of 116 total aligned reads displayed.

TCAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTT  >  NC_000913/144660‑144957

tcAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCatt                                                                                                                                                      <  2:738908/150‑1 (MQ=255)

 cAGTGCCGGGCCGTCAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGGGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCatta                                                                                                                                                     <  1:953360/150‑1 (MQ=255)

       cGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGc                                                                                                                                               >  2:811227/1‑150 (MQ=255)

          gCCGTGAATTTCTGGCGCGGATTAAGCTATAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCcac                                                                                                                                            <  2:1691948/150‑1 (MQ=255)

            cGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTAATAATGAAGCcacgc                                                                                                                                          <  1:669806/150‑3 (MQ=255)

            cGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGAAATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACaa                                                                                                                                          >  2:311078/1‑150 (MQ=255)

              tGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAac                                                                                                                                        <  1:459198/150‑1 (MQ=255)

               gAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAaca                                                                                                                                       >  2:365499/1‑150 (MQ=255)

                 aTTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACt                                                                                                                                     >  1:1353296/1‑150 (MQ=255)

                    tCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTccc                                                                                                                                  <  2:1262130/150‑1 (MQ=255)

                         cgcgGATTAAGCGCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGc                                                                                                                             <  1:1548399/150‑1 (MQ=255)

                            ggATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGAGaa                                                                                                                          >  2:1445048/1‑150 (MQ=255)

                            ggATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATAGCGTGTCTTCATTATTAAGCAACAAACACTCCCGTTGCGaa                                                                                                                          >  2:672938/1‑150 (MQ=255)

                               ttAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTg                                                                                                                       <  1:1727875/150‑1 (MQ=255)

                                 aaGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGAt                                                                                                                     <  1:1480479/150‑1 (MQ=255)

                                  aGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGAtt                                                                                                                    >  1:1335898/1‑150 (MQ=255)

                                   gCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGAttt                                                                                                                   >  1:91102/1‑150 (MQ=255)

                                      ctAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTg                                                                                                                <  1:1441264/150‑1 (MQ=255)

                                      ctAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCAGCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTg                                                                                                                <  2:1286267/150‑1 (MQ=255)

                                       tAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTgg                                                                                                               <  1:753422/150‑1 (MQ=255)

                                       tAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTgg                                                                                                               <  1:1606900/150‑1 (MQ=255)

                                          tatGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGTGGAATCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGgtgt                                                                                                            <  2:1061043/150‑1 (MQ=255)

                                            tGCTCAGCCGCATGTTGTGCGATGCTCTGCATGTAGCGGACTCTGGTGAGGGTGTCATCTTCTTTACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCa                                                                                                          <  1:302730/150‑1 (MQ=255)

                                            tGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCa                                                                                                          >  2:928999/1‑150 (MQ=255)

                                              cgcAGCCGCATGATGGGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACg                                                                                                        <  1:406238/148‑1 (MQ=255)

                                                  gCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTAc                                                                                                    >  2:6836/1‑150 (MQ=255)

                                                  gCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTAc                                                                                                    <  1:1062333/150‑1 (MQ=255)

                                                   ccGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTAcc                                                                                                   <  1:1257335/150‑1 (MQ=255)

                                                    cGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCg                                                                                                  <  2:1353296/150‑1 (MQ=255)

                                                    cGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCg                                                                                                  <  2:299425/150‑1 (MQ=255)

                                                     gCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACACCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGt                                                                                                 >  2:357738/1‑150 (MQ=255)

                                                      cATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGtt                                                                                                <  2:39682/150‑1 (MQ=255)

                                                      cATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGtt                                                                                                <  2:971689/150‑1 (MQ=255)

                                                        tgatgTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTaa                                                                                              >  2:1086991/1‑150 (MQ=255)

                                                         gatgTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGTGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAAt                                                                                             <  2:709506/150‑1 (MQ=255)

                                                         gatgTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAAt                                                                                             <  2:1486224/150‑1 (MQ=255)

                                                            gtgCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGa                                                                                          <  1:331531/150‑1 (MQ=255)

                                                              gCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAAc                                                                                        <  1:274707/150‑1 (MQ=255)

                                                                gATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAg                                                                                      <  2:1592247/150‑1 (MQ=255)

                                                                 aTGCTCTGCATGTAGCTGAGTCTGGTTAGGCTGTCATCTTCTTAACCGATATAGACGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCAACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAga                                                                                     <  1:258210/150‑1 (MQ=255)

                                                                  tGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAgat                                                                                    <  1:1707009/150‑1 (MQ=255)

                                                                     tctGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCCCCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAgatgat                                                                                 >  1:1225239/1‑150 (MQ=255)

                                                                      cttcATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATATCCGGAGCGCCACCGTTTCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGatgatg                                                                                <  1:311078/147‑1 (MQ=255)

                                                                         cATGAAGTGGACTCTGGTGAGGGTGTTATCTTCTTTAGCGATATAGCCGGAGCGCATACGTATAGTGTTGCTTCATTTTTAAGCTACAAACACTCCCGTTGCGTAGTGATTTCTGGTGTCACGTTAACGTTAATTGAACAGATGATGGCt                                                                             <  2:1116976/150‑1 (MQ=255)

                                                                           tGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCTGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTg                                                                           <  2:669528/150‑1 (MQ=255)

                                                                            gAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGc                                                                          <  1:1049280/150‑1 (MQ=255)

                                                                            gAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGc                                                                          <  1:457796/150‑1 (MQ=255)

                                                                            gAAGCGGACTCTCGTGAGGGTGTCATCTTCTAAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATAGAACAGATGATGTCTTGc                                                                          >  2:1528052/1‑150 (MQ=255)

                                                                             aaGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGcc                                                                         <  2:1212622/150‑1 (MQ=255)

                                                                              aGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCg                                                                        <  1:1657642/150‑1 (MQ=255)

                                                                              aGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCg                                                                        <  1:1664254/150‑1 (MQ=255)

                                                                              aGCGGACTCTGGTGAGGGTCGCATCTTCTTAAACGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACTCTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCg                                                                        <  1:263088/150‑1 (MQ=255)

                                                                                    ctctGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAAc                                                                  >  2:790954/1‑150 (MQ=255)

                                                                                     tctGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAAcc                                                                 <  2:1340526/150‑1 (MQ=255)

                                                                                      cttgTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTCTCGCGGGGCTTCATTATTACGCCACAAACACTCCCTTTTCGAATTGATTTCTGGCGTCACGCTACCGTTAATGGAACAGATGCGGGCCTGCCGTGAAACCa                                                                <  2:289298/147‑1 (MQ=255)

                                                                                      ctGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCa                                                                <  2:1694635/150‑1 (MQ=255)

                                                                                        ggTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATg                                                              <  2:749300/150‑1 (MQ=255)

                                                                                           gAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGAcc                                                           <  1:470892/150‑1 (MQ=255)

                                                                                                tgtCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTc                                                      <  2:871658/150‑1 (MQ=255)

                                                                                                  tCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACGATTTCat                                                    >  1:1075565/1‑149 (MQ=255)

                                                                                                    aTCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCagag                                                  <  1:1098556/150‑1 (MQ=255)

                                                                                                    aTCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCagag                                                  <  1:1545428/150‑1 (MQ=255)

                                                                                                     tcttctTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTGGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGt                                                 <  2:91102/150‑1 (MQ=255)

                                                                                                        tcttAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTc                                              >  2:627288/1‑150 (MQ=255)

                                                                                                        tcttAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTc                                              <  2:1518901/150‑1 (MQ=255)

                                                                                                          ttAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTcgc                                            <  1:709965/150‑1 (MQ=255)

                                                                                                           tAACCGATATAGCCGGATCGCCACCGTATCGCGTGGCTGCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGGCACGTTACCGTTAATTGAACAGATGATGGCGTGCCGTGAAACCATGACCAGTTCAGAGTTTcgcg                                           <  1:1047881/150‑1 (MQ=255)

                                                                                                           tAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCATCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTcgcg                                           >  2:724734/1‑150 (MQ=255)

                                                                                                            aaCCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGa                                          >  2:1116927/1‑150 (MQ=255)

                                                                                                             aCCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAACCCATGACCAGTTCAGAGTTTCGCGAg                                         >  2:233813/1‑150 (MQ=255)

                                                                                                             aCCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAg                                         <  2:1472178/150‑1 (MQ=255)

                                                                                                             aCCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAg                                         <  2:1186169/150‑1 (MQ=255)

                                                                                                              ccGATATAGCCGGAGCGACACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATAGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGc                                        >  2:1724038/1‑150 (MQ=255)

                                                                                                                 atatAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTa                                     <  1:811227/150‑1 (MQ=255)

                                                                                                                  tataGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTAt                                    >  1:476249/1‑150 (MQ=255)

                                                                                                                  tataGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTAt                                    <  2:569659/150‑1 (MQ=255)

                                                                                                                    taGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTg                                  >  2:700464/1‑150 (MQ=255)

                                                                                                                     aGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGt                                 >  2:1686494/1‑150 (MQ=255)

                                                                                                                     aGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGt                                 >  1:1437241/1‑150 (MQ=255)

                                                                                                                      gCCGGAGCGCCACCGTATCGCTTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTc                                <  2:1524237/150‑1 (MQ=255)

                                                                                                                       ccGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCg                               <  1:998822/150‑1 (MQ=255)

                                                                                                                       ccGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGAGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCg                               >  2:792295/1‑150 (MQ=255)

                                                                                                                        cGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTTCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGa                              <  1:1329265/150‑1 (MQ=255)

                                                                                                                                cACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTg                      <  1:1086991/150‑1 (MQ=255)

                                                                                                                                 aCCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCTAGCGTATTGTCGAACTGGGTgc                     >  2:1428756/1‑150 (MQ=255)

                                                                                                                                 aCCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTgc                     <  1:159394/150‑1 (MQ=255)

                                                                                                                                    gTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGcc                  <  1:700464/150‑1 (MQ=255)

                                                                                                                                      aTCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCAGGTGTCACGTTACCGTTAATTGAACAGATGATGCCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCTAGCGTATTGTCGAACAGGGTGCGCCgg                >  1:1274660/1‑150 (MQ=255)

                                                                                                                                       tCGCGTGGCTTCATTATTAAG‑CACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAg              <  2:959196/150‑1 (MQ=255)

                                                                                                                                       gtttgTGGCTTAATAATGAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAACCCAAGACCAGTTCAGACTTTCGCGAGCGTATTGTCGAACAGGGTGCGCCGGa               >  2:786260/5‑150 (MQ=255)

                                                                                                                                       gtttgTGGCTTAATAATGAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGa               >  1:1172775/5‑150 (MQ=255)

                                                                                                                                        tttgTGGCTTAATAATGAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAg              >  1:968515/4‑150 (MQ=255)

                                                                                                                                         gcgTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAgg             >  1:144174/1‑150 (MQ=255)

                                                                                                                                           gTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTg           <  1:365499/150‑1 (MQ=255)

                                                                                                                                            gggCTTCATAATTAAGCCAAAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGa          <  1:760609/149‑1 (MQ=255)

                                                                                                                                               cTTCAATATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTAACGTTAATTGAACAGATGATGGATTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCTAGCGTATTGTCGAACAGGGTGCGCCGGAGGTCagta       >  1:47958/1‑150 (MQ=255)

                                                                                                                                                ttCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGagtag      <  1:851093/150‑1 (MQ=255)

                                                                                                                                                  cATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTc    >  1:955400/1‑150 (MQ=255)

                                                                                                                                                   attattAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCt   <  2:1069905/150‑1 (MQ=255)

                                                                                                                                                    ttattaAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGGCACGGTACCTTTAATTGAACAGATGATGGCTTGCCGGGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCtt  <  2:861954/150‑1 (MQ=255)

TCAGTGCCGGGCCGTGAATTTCTGGCGCGGATTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATGAAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTATCGCGTGGCTTCATTATTAAGCCACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCATGACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGCGCCGGAGGTGAGTAGTCTT  >  NC_000913/144660‑144957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`