Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,602,239 A→T 5.9% K250N (AAA→AAT lsrA → autoinducer 2 import ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,602,2390AT 5.9% 255.5 / 3.0 87K250N (AAA→AATlsrAautoinducer 2 import ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (40/41);  new base T (3/3);  total (43/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGGCTGGGCTGGTGGG  >  NC_000913/1602092‑1602385
                                                                                                                                                   |                                                                                                                                                  
ggAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAAtt                                                                                                                                                  >  1:495258/1‑150 (MQ=255)
ggAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAAtt                                                                                                                                                  <  1:1505647/150‑1 (MQ=255)
   aaTTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATg                                                                                                                                               <  2:333178/150‑1 (MQ=255)
     ttCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCATAATTTTTGGc                                                                                                                                             <  1:922552/150‑1 (MQ=255)
     ttCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGc                                                                                                                                             <  2:1129918/150‑1 (MQ=255)
     ttCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGc                                                                                                                                             <  2:192072/150‑1 (MQ=255)
      tCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCt                                                                                                                                            <  2:445589/150‑1 (MQ=255)
        gCCAGATTGCCGATCGAATTAGCGTGATGCGCGACTGAAGCATCGGCTTAAGCGGCAAAACCAGCGCACTGTCTTCCGACGCCATTATTCCGGCCATCCCCCCAGCGGTACGGGAACACTCGCTCTCTGCCCGCCAAAAATTATTGCttg                                                                                                                                          >  1:288711/1‑148 (MQ=255)
        gCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTgg                                                                                                                                          >  1:800916/1‑150 (MQ=255)
           aGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGATAAATAGCTCTCTGCCAGCCAAAAATTATGGCTGGAGt                                                                                                                                       <  2:1317952/150‑1 (MQ=255)
                gCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCt                                                                                                                                  <  1:981394/150‑1 (MQ=255)
                gCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCt                                                                                                                                  >  1:941798/1‑150 (MQ=255)
                   gctCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCGCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGt                                                                                                                               <  2:36803/148‑1 (MQ=255)
                   gATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGt                                                                                                                               <  2:18666/150‑1 (MQ=255)
                   gATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGt                                                                                                                               >  2:353534/1‑150 (MQ=255)
                     tCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCCGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTaa                                                                                                                             >  2:112095/1‑150 (MQ=255)
                       gacgtcgCGTGATGCGCTACGGAAACATCGCCTAAACCGGCAAAACCAGCGAACTGTCTACCGAGGACTTTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCATCCAAAAATAATGGCTGGAGTTACCTGGTAAcc                                                                                                                           <  2:339889/144‑1 (MQ=255)
                          ttAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGcc                                                                                                                        <  2:508341/150‑1 (MQ=255)
                             gCGTGATGCGCGACGGAACCCTCGCCTTAAGCGGCAAACCCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAACTCGCTCTCTGCCAGCCAAACATTATGGCTGGAGTTACCTGGTAACCGCCcac                                                                                                                     <  2:392573/150‑1 (MQ=255)
                             gCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGGCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCcac                                                                                                                     <  2:1058441/150‑1 (MQ=255)
                              cGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAACCCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAAGCGCGCTCTGCCCGCCAAGAATTATGGCTGGAGTTACCTGGTAACCGCCcacc                                                                                                                    >  1:372405/1‑149 (MQ=255)
                                 gATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCacaaca                                                                                                                 <  2:1246486/150‑1 (MQ=255)
                                   tGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATg                                                                                                               >  2:1028576/1‑150 (MQ=255)
                                    gcgcGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGCCGACATTATTCAGGCCATCACCCCAGCGGTACCGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTGACCTGGTAACCGCCCACAACATgc                                                                                                              >  2:730937/1‑150 (MQ=255)
                                    gcgcGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATgc                                                                                                              <  2:307190/150‑1 (MQ=255)
                                        gACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATgccgcc                                                                                                          >  2:1503400/1‑150 (MQ=255)
                                              aCCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACg                                                                                                    >  1:174019/1‑150 (MQ=255)
                                                 aTCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCg                                                                                                 >  2:1176041/1‑150 (MQ=255)
                                                       ttAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTg                                                                                           <  2:1673714/150‑1 (MQ=255)
                                                       ttAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTg                                                                                           <  2:711905/150‑1 (MQ=255)
                                                       ttAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTAAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTAACCTGGTAACCGCCCACACCATGCCGCCGGAACGCCGGAGCTg                                                                                           >  1:639849/1‑150 (MQ=255)
                                                         aaGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGGACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGac                                                                                         <  2:1640010/150‑1 (MQ=255)
                                                          aGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCt                                                                                            <  2:903658/146‑1 (MQ=255)
                                                          aGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCt                                                                                            >  1:903658/1‑146 (MQ=255)
                                                           gCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGATAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGacac                                                                                       <  1:689664/150‑1 (MQ=255)
                                                             ggCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTg                                                                                     >  1:772585/1‑150 (MQ=255)
                                                              gCAAAACCTGCGAACTGTTTAACGACGACATTTTTCAGGCCATCAACCCAGCGGTACTGGAAAAATCGCTCTCTGCCAGCCAAAAATTATTGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTgg                                                                                    <  2:288711/150‑1 (MQ=255)
                                                              gCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTgg                                                                                    >  2:1507151/1‑150 (MQ=255)
                                                              gCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTgg                                                                                    <  2:495258/150‑1 (MQ=255)
                                                               cAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTACCCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGa                                                                                   >  1:1084037/1‑150 (MQ=255)
                                                               cAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCGGGAGTTACCTGGTAACCGCCCCCAACAGGCCGCAGGAACGCCGGTGCTGACACTggc                                                                                   >  2:476426/1‑149 (MQ=255)
                                                                aaaaCCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGaa                                                                                  >  2:1484958/1‑150 (MQ=255)
                                                                 aaaCCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGaaa                                                                                 <  2:767546/150‑1 (MQ=255)
                                                                  aaCCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGaaaa                                                                                >  1:165586/1‑150 (MQ=255)
                                                                   aCCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCACCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCGGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGGGCGGACACTGGAAAAt                                                                               <  2:551182/150‑1 (MQ=255)
                                                                     cAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTTCTGGAAAAATCGCTCTCTGCCAGCCAACAATTATGGCTGGAGTTACCAGGTACCCGCGCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCt                                                                             >  1:1254154/1‑150 (MQ=255)
                                                                             tGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCg                                                                     >  2:1357293/1‑150 (MQ=255)
                                                                              gTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCgg                                                                    >  2:1439488/1‑149 (MQ=255)
                                                                              gTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGa                                                                    >  1:586710/1‑150 (MQ=255)
                                                                                  aCCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGt                                                                >  1:1159095/1‑150 (MQ=255)
                                                                                    cgacgaCATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCTCCCACAACATGCCGCCGGAACGCCCGTGCTGACACTGGAAAATCTGACCGGCGAAGGatt                                                              >  1:1405998/1‑150 (MQ=255)
                                                                                     gacgacATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTc                                                             <  2:174019/150‑1 (MQ=255)
                                                                                       cgacATTATTCAGGCCATCACCCCAGCGGTACGGGAAAACTCGCTCTCTGCCAGCCAAAATTTATGGCTGGAGTTACCTGGTAACCGCCCACAACCTGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAg                                                           <  2:934957/150‑1 (MQ=255)
                                                                                       cgacATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAg                                                           <  2:423190/150‑1 (MQ=255)
                                                                                       cgacATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAg                                                           <  2:1722418/150‑1 (MQ=255)
                                                                                       cgacATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAg                                                           <  1:1503400/150‑1 (MQ=255)
                                                                                       cgacATTATTCAGGCCAGCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAAACGCACACTACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAg                                                           <  1:1562304/150‑1 (MQ=255)
                                                                                          cATTATTCAGGCCATCCCCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACCACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGaa                                                        >  1:700133/1‑150 (MQ=255)
                                                                                           attattCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAAt                                                       <  2:586710/150‑1 (MQ=255)
                                                                                            ttattCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATg                                                      <  2:378199/150‑1 (MQ=255)
                                                                                              attCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTc                                                    <  2:564742/150‑1 (MQ=255)
                                                                                               ttCAGGCCATCACCCCTGCGGTACGGGAAAAATCGCTCTCTGCCAGCCTAAATTTATGGCTGGAGTTACCTTGTAACCGCCCACAACATTCCGCCGTAACGCCGGTGCTGACACTGTAAAATCTGACCGGCGAAGGTTTCAGGAATGTCa                                                   <  1:897617/150‑1 (MQ=255)
                                                                                               ttCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACCTGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCa                                                   >  1:1575978/1‑150 (MQ=255)
                                                                                                   ggCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAATATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGCGACTGGCAAATCTGACGGGCGTAGGTTTGAGGAATGTCCGcag                                               >  1:1562982/1‑148 (MQ=255)
                                                                                                   ggCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGACCGCCGGTGCTGACACTGGATAATCTGACCGGCGATGGTTTGAGGAATGTCAGCCt                                               >  2:215539/1‑150 (MQ=255)
                                                                                                   ggCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGAGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCt                                               <  1:1176041/150‑1 (MQ=255)
                                                                                                     ccATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCAACAACCTGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTgc                                             >  1:37246/1‑149 (MQ=255)
                                                                                                      cATCACCCCAGCGGTACGGGTAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCAACAACATGCCGCCGGAACGCCGGTGCTGACCCTGGAAAATCTGACCGGCGATGGTTTGAGGAATGTCAGCCTGAc                                            >  2:820057/1‑150 (MQ=255)
                                                                                                       aTCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACg                                           <  1:592776/150‑1 (MQ=255)
                                                                                                        tCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGc                                          >  1:1189148/1‑150 (MQ=255)
                                                                                                         cACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGAGCTGACACTGGAAAATCTGACCGGCGAAGGTTGCAGCAAGGTCAGCCTGACGcg                                         >  2:460493/1‑149 (MQ=255)
                                                                                                          aCCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTc                                        <  2:800916/150‑1 (MQ=255)
                                                                                                                 cGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCg                                 <  1:1125994/150‑1 (MQ=255)
                                                                                                                         gAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAAtt                         >  1:794595/1‑150 (MQ=255)
                                                                                                                            aaaTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTg                      <  2:941798/150‑1 (MQ=255)
                                                                                                                            aaaTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGGTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTg                      <  2:1159552/150‑1 (MQ=255)
                                                                                                                             aaTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTgg                     <  1:459872/150‑1 (MQ=255)
                                                                                                                              aTCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTggg                    <  2:1091132/150‑1 (MQ=255)
                                                                                                                               tCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGCTAACCGCCCACAACATGCCGCCGGAACGCCGGAGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGc                   >  2:1593792/1‑150 (MQ=255)
                                                                                                                                 gCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCct                 >  1:1294840/1‑150 (MQ=255)
                                                                                                                                    taactccagccaTAATTTTTGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCctggc              >  1:1365359/6‑150 (MQ=255)
                                                                                                                                      ctGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCctggctg            >  1:547453/1‑150 (MQ=255)
                                                                                                                                      actccagccaTAATTTTTGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCctggctg            >  1:742511/4‑150 (MQ=255)
                                                                                                                                         ccagccaTAATTTTTGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATCTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTggctgggc         >  1:1220903/1‑150 (MQ=255)
                                                                                                                                              caAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGGCTGGGCtggtg    >  2:1193021/1‑150 (MQ=255)
                                                                                                                                              caAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGGCTGGGCtggtg    >  1:784230/1‑150 (MQ=255)
                                                                                                                                              caAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGGCTGGGCtggtg    <  1:566963/150‑1 (MQ=255)
                                                                                                                                                aaaaTTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGGCTGGGCTGGTggg  <  2:845024/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAAAATCGCTCTCTGCCAGCCAAAAATTATGGCTGGAGTTACCTGGTAACCGCCCACAACATGCCGCCGGAACGCCGGTGCTGACACTGGAAAATCTGACCGGCGAAGGTTTCAGGAATGTCAGCCTGACGCTCAATGCCGGAGAAATTCTGGGCCTGGCTGGGCTGGTGGG  >  NC_000913/1602092‑1602385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: