Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,609,200 C→T 7.0% intergenic (‑178/+29) yneE ← / ← uxaB bestrophin family putative inner membrane protein/altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,609,2000CT 7.0% 308.8 / 7.1 99intergenic (‑178/+29)yneE/uxaBbestrophin family putative inner membrane protein/altronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base C (38/54);  new base T (2/5);  total (40/59)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

CTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCT  >  NC_000913/1609051‑1609349
                                                                                                                                                      |                                                                                                                                                     
cTATTATACGTTATTACGATCACATACTCAAGACGCTACTTACAAATTACCTACAACCTTACAACATTAGCAGCCGCTGCAGGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTc                                                                                                                                                       <  2:90371/150‑1 (MQ=255)
cTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTc                                                                                                                                                       <  2:1187552/150‑1 (MQ=255)
cTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTc                                                                                                                                                       <  1:583478/150‑1 (MQ=255)
 tATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCg                                                                                                                                                      >  1:480729/1‑150 (MQ=255)
     aaaCGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAAc                                                                                                                                                  >  1:35354/1‑150 (MQ=255)
        cGTGATTACGATCACATTCTCAAGACGCTGCTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGTCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCgg                                                                                                                                               >  1:198722/1‑150 (MQ=255)
        cGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCgg                                                                                                                                               <  1:397000/150‑1 (MQ=255)
         gTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCggg                                                                                                                                              <  2:654166/150‑1 (MQ=255)
         gTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCggg                                                                                                                                              >  1:398270/1‑150 (MQ=255)
         gTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCggg                                                                                                                                              <  1:258104/150‑1 (MQ=255)
         gTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCggg                                                                                                                                              <  2:1417503/150‑1 (MQ=255)
         gTGATTAAGTTCACATTCTCTAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCggg                                                                                                                                              <  2:238620/150‑1 (MQ=255)
            aTTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTc                                                                                                                                           <  1:402486/150‑1 (MQ=255)
             ttACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTTTCGACCGGGtttc                                                                                                                                          <  1:1597186/150‑3 (MQ=255)
              tACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTTTCGACCGGGtttct                                                                                                                                         <  1:1110971/150‑4 (MQ=255)
              tACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTTTCGACCGGGtttct                                                                                                                                         <  1:1612987/150‑4 (MQ=255)
                 gATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAg                                                                                                                                      <  2:1130516/150‑1 (MQ=255)
                 gATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAg                                                                                                                                      <  1:775233/150‑1 (MQ=255)
                 gATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAg                                                                                                                                      <  2:1501487/150‑1 (MQ=255)
                 gATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAg                                                                                                                                      >  1:706988/1‑150 (MQ=255)
                  aTCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGt                                                                                                                                     >  2:1111823/1‑150 (MQ=255)
                    cacaTTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTaa                                                                                                                                   >  1:984154/1‑150 (MQ=255)
                         tctcAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGgtg                                                                                                                              <  2:491822/150‑1 (MQ=255)
                          ctcAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGGTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGgtgt                                                                                                                             >  1:797912/1‑150 (MQ=255)
                              aGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGttatt                                                                                                                         <  2:484420/150‑1 (MQ=255)
                                aCGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAg                                                                                                                       <  1:1416938/150‑1 (MQ=255)
                                   cTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGcac                                                                                                                    <  1:804057/150‑1 (MQ=255)
                                      cTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAAc                                                                                                                 >  1:604938/1‑150 (MQ=255)
                                      attACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATATGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAAc                                                                                                                 <  2:1378586/149‑1 (MQ=255)
                                       ttACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGTCATATAGGATGTTGTAACTAATATGGTGAAATAAGATACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACg                                                                                                                <  1:1127144/150‑1 (MQ=255)
                                       ttACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACg                                                                                                                <  2:173834/150‑1 (MQ=255)
                                         aCAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTTTCGACCGGGtttct                                                                                                                                         <  2:867290/123‑4 (MQ=255)
                                         aCAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTTTCGACCGGGtttct                                                                                                                                         >  1:867290/1‑120 (MQ=255)
                                          cAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGAc                                                                                                             <  2:1392371/150‑1 (MQ=255)
                                            aaTTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGt                                                                                                           >  1:587112/1‑150 (MQ=255)
                                             aatACCTACAAGCTTACAACATTAGCAGGCGATGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACAAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTa                                                                                                          <  1:69303/148‑1 (MQ=255)
                                                aCCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACa                                                                                                        <  1:399845/150‑1 (MQ=255)
                                                  cTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTTTCGACCGGGtttctt                                                                                                                                        >  1:519808/1‑111 (MQ=255)
                                                  cTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTTTCGACCGGGtttctt                                                                                                                                        <  2:519808/115‑5 (MQ=255)
                                                    aCAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGCTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTc                                                                                                   >  2:23703/1‑150 (MQ=255)
                                                       aGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTcgcg                                                                                                >  2:1461580/1‑150 (MQ=255)
                                                         cctttatcaTTAACAGGTGCCACATGCAGCGCCTGCTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCTCAACGGACGTACAGCTTCGCGCa                                                                                              >  1:376156/8‑150 (MQ=37)
                                                         cTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCa                                                                                              <  1:1039004/150‑1 (MQ=255)
                                                          ttACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCAt                                                                                             <  1:1111823/150‑1 (MQ=255)
                                                             tatcaTTAACAGGTGCCACATGCAGCGCCTGCTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACAGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATccc                                                                                          >  1:143191/4‑150 (MQ=38)
                                                              aacaTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCt                                                                                         <  2:5013/150‑1 (MQ=255)
                                                                   tAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTc                                                                                    <  2:913034/150‑1 (MQ=255)
                                                                    aGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCg                                                                                   <  2:1081092/150‑1 (MQ=255)
                                                                     ccAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCga                                                                                  <  2:1274981/149‑1 (MQ=255)
                                                                       tggCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTCAAATAAGAAACCCGGTCGCATCCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCgaga                                                                                <  2:1053645/149‑1 (MQ=255)
                                                                        ggCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGaa                                                                               <  1:853720/150‑1 (MQ=255)
                                                                        ggCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGaa                                                                               >  1:441555/1‑150 (MQ=255)
                                                                          cgcTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATc                                                                             <  1:1078477/150‑1 (MQ=255)
                                                                            cTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGc                                                                           >  1:619952/1‑150 (MQ=255)
                                                                               cATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATc                                                                        >  2:23093/1‑150 (MQ=255)
                                                                               cATGTGGCACCTGTTAAAGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGTTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTAAGAGAATCGCATc                                                                        >  1:276131/1‑150 (MQ=255)
                                                                                aTGTGGCACCTGTTAATGATAAATGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCCAAACCGGGTTCAGAAGTAACGGTGTAATTAGCCCAACGGACGTACCGCTTCGCGCACCCCTTTTTCGAGAATCGCATcc                                                                       <  2:82101/150‑1 (MQ=255)
                                                                                 tgtgGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCa                                                                      >  1:930425/1‑150 (MQ=255)
                                                                                 tgtgGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAg                                                                     >  1:996669/1‑150 (MQ=255)
                                                                                   tgGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAgg                                                                    >  1:1158130/1‑150 (MQ=255)
                                                                                    ggCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGt                                                                   >  2:154548/1‑150 (MQ=255)
                                                                                         cTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTa                                                              >  1:1485105/1‑150 (MQ=255)
                                                                                          tGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACTGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCAATAg                                                             >  1:98318/1‑150 (MQ=255)
                                                                                          tGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAg                                                             <  1:749732/150‑1 (MQ=255)
                                                                                              aaTGTTATAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAAGCGGTTTCAGAAGTAACGGTGTTATTAGCACATCGGACGTACAGCTACGCGCATCCAGTTTTCGAGAATCGCATCCAGGTCATTAGCacc                                                         >  2:1131008/1‑148 (MQ=255)
                                                                                               aTGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAAcc                                                        <  2:1465839/150‑1 (MQ=255)
                                                                                               aTGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAAcc                                                        >  1:1430679/1‑150 (MQ=255)
                                                                                               aTGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAAcc                                                        <  1:1284211/150‑1 (MQ=255)
                                                                                                    aaaGGCATATAGTATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTc                                                   <  1:373690/150‑1 (MQ=255)
                                                                                                    aaaGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCta                                                   >  2:896044/1‑149 (MQ=255)
                                                                                                    aaaGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTc                                                   <  2:198722/150‑1 (MQ=255)
                                                                                                     aaGGAATATAGGATGTTGTAACTAATATGGTGATATATGAATCCCGTTCGAAACAGGGTTCAGAAGTAACGGTGTTATTAGCACTACGTACGTACAGCTTCGTGCTTCGCTTTTTCGAGATTCGCATCCAGGTCTTTAGCTACGTGCTCg                                                  <  1:1107333/150‑1 (MQ=255)
                                                                                                       ggCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgac                                                <  1:521681/150‑1 (MQ=255)
                                                                                                           tataGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgaccaga                                            <  2:1232136/150‑1 (MQ=255)
                                                                                                             taGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgaccagacc                                          >  1:1515711/1‑150 (MQ=255)
                                                                                                               ggATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGaccagaccag                                        <  2:1636637/150‑1 (MQ=255)
                                                                                                                gATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAgg                                       <  1:1021624/150‑1 (MQ=255)
                                                                                                                gATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGGGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAgg                                       <  1:193795/150‑1 (MQ=255)
                                                                                                                    ttgtAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACt                                   <  2:638047/150‑1 (MQ=255)
                                                                                                                    ttgtAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACt                                   >  2:465885/1‑150 (MQ=255)
                                                                                                                    ttgtAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACt                                   <  1:1718828/150‑1 (MQ=255)
                                                                                                                      gtAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTg                                 >  2:548076/1‑150 (MQ=255)
                                                                                                                       tAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGc                                <  2:1376704/150‑1 (MQ=255)
                                                                                                                          cTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTTCTCGACCAGACCAGGCTCTTGCGTc                             >  1:377140/1‑150 (MQ=255)
                                                                                                                          cTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTc                             <  2:732990/150‑1 (MQ=255)
                                                                                                                            aaTATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAAACGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCCCGTGGGTCAg                           >  2:1129821/1‑150 (MQ=255)
                                                                                                                               atGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTc                        >  2:988696/1‑150 (MQ=255)
                                                                                                                                tGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTcc                       <  1:1396834/150‑1 (MQ=255)
                                                                                                                                   tGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGc                    >  2:563627/1‑150 (MQ=255)
                                                                                                                                    gAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCt                   >  1:429936/1‑150 (MQ=255)
                                                                                                                                          aGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGt             <  2:629171/150‑1 (MQ=255)
                                                                                                                                          aGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGt             <  2:706988/150‑1 (MQ=255)
                                                                                                                                          aGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGt             <  2:398270/150‑1 (MQ=255)
                                                                                                                                             aaaCCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTgg           <  2:984154/150‑1 (MQ=255)
                                                                                                                                              aaCCCGGTCGAAACCGGGTTCAGAAGTAACGGCGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGt          >  1:373418/1‑150 (MQ=255)
                                                                                                                                               aCCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTc         <  2:488454/150‑1 (MQ=255)
                                                                                                                                                 ccGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCtt       >  1:621436/1‑150 (MQ=255)
                                                                                                                                                   ggTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCtttt     <  1:392377/150‑1 (MQ=255)
                                                                                                                                                     tCGAATCCGGGTTCTGAAGTAAGGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGATTCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTTGTCTTTTTc   <  1:909775/150‑1 (MQ=255)
                                                                                                                                                      cGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCACCCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCt  >  2:881985/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCT  >  NC_000913/1609051‑1609349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: