Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,609,201 G→T 7.1% intergenic (‑179/+28) yneE ← / ← uxaB bestrophin family putative inner membrane protein/altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,609,2010GT 7.1% 299.0 / 10.0 99intergenic (‑179/+28)yneE/uxaBbestrophin family putative inner membrane protein/altronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (38/53);  new base T (2/5);  total (40/59)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.68e-01

Only 100 of 102 total aligned reads displayed.

TATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTG  >  NC_000913/1609052‑1609350
                                                                                                                                                      |                                                                                                                                                     
tATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCg                                                                                                                                                       >  1:480729/1‑150 (MQ=255)
    aaaCGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAAc                                                                                                                                                   >  1:35354/1‑150 (MQ=255)
       cGTGATTACGATCACATTCTCAAGACGCTGCTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGTCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCgg                                                                                                                                                >  1:198722/1‑150 (MQ=255)
       cGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCgg                                                                                                                                                <  1:397000/150‑1 (MQ=255)
        gTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCggg                                                                                                                                               <  2:654166/150‑1 (MQ=255)
        gTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCggg                                                                                                                                               <  1:258104/150‑1 (MQ=255)
        gTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCggg                                                                                                                                               >  1:398270/1‑150 (MQ=255)
        gTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCggg                                                                                                                                               <  2:1417503/150‑1 (MQ=255)
        gTGATTAAGTTCACATTCTCTAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCggg                                                                                                                                               <  2:238620/150‑1 (MQ=255)
           aTTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTc                                                                                                                                            <  1:402486/150‑1 (MQ=255)
            ttACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTTTCGACCGGGtttc                                                                                                                                           <  1:1597186/150‑3 (MQ=255)
             tACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTTTCGACCGGGtttct                                                                                                                                          <  1:1110971/150‑4 (MQ=255)
             tACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTTTCGACCGGGtttct                                                                                                                                          <  1:1612987/150‑4 (MQ=255)
                gATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAg                                                                                                                                       <  2:1501487/150‑1 (MQ=255)
                gATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAg                                                                                                                                       >  1:706988/1‑150 (MQ=255)
                gATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAg                                                                                                                                       <  1:775233/150‑1 (MQ=255)
                gATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAg                                                                                                                                       <  2:1130516/150‑1 (MQ=255)
                 aTCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGt                                                                                                                                      >  2:1111823/1‑150 (MQ=255)
                   cacaTTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTaa                                                                                                                                    >  1:984154/1‑150 (MQ=255)
                        tctcAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGgtg                                                                                                                               <  2:491822/150‑1 (MQ=255)
                         ctcAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGGTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGgtgt                                                                                                                              >  1:797912/1‑150 (MQ=255)
                             aGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGttatt                                                                                                                          <  2:484420/150‑1 (MQ=255)
                               aCGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAg                                                                                                                        <  1:1416938/150‑1 (MQ=255)
                                  cTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGcac                                                                                                                     <  1:804057/150‑1 (MQ=255)
                                     cTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAAc                                                                                                                  >  1:604938/1‑150 (MQ=255)
                                     attACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATATGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAAc                                                                                                                  <  2:1378586/149‑1 (MQ=255)
                                      ttACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGTCATATAGGATGTTGTAACTAATATGGTGAAATAAGATACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACg                                                                                                                 <  1:1127144/150‑1 (MQ=255)
                                      ttACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACg                                                                                                                 <  2:173834/150‑1 (MQ=255)
                                        aCAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTTTCGACCGGGtttct                                                                                                                                          <  2:867290/123‑4 (MQ=255)
                                        aCAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTTTCGACCGGGtttct                                                                                                                                          >  1:867290/1‑120 (MQ=255)
                                         cAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGAc                                                                                                              <  2:1392371/150‑1 (MQ=255)
                                           aaTTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGt                                                                                                            >  1:587112/1‑150 (MQ=255)
                                            aatACCTACAAGCTTACAACATTAGCAGGCGATGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACAAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTa                                                                                                           <  1:69303/148‑1 (MQ=255)
                                               aCCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACa                                                                                                         <  1:399845/150‑1 (MQ=255)
                                                 cTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTTTCGACCGGGtttctt                                                                                                                                         >  1:519808/1‑111 (MQ=255)
                                                 cTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTTTCGACCGGGtttctt                                                                                                                                         <  2:519808/115‑5 (MQ=255)
                                                   aCAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGCTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTc                                                                                                    >  2:23703/1‑150 (MQ=255)
                                                      aGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTcgcg                                                                                                 >  2:1461580/1‑150 (MQ=255)
                                                        cctttatcaTTAACAGGTGCCACATGCAGCGCCTGCTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCTCAACGGACGTACAGCTTCGCGCa                                                                                               >  1:376156/8‑150 (MQ=37)
                                                        cTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCa                                                                                               <  1:1039004/150‑1 (MQ=255)
                                                         ttACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCAt                                                                                              <  1:1111823/150‑1 (MQ=255)
                                                            tatcaTTAACAGGTGCCACATGCAGCGCCTGCTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACAGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATccc                                                                                           >  1:143191/4‑150 (MQ=38)
                                                             aacaTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCt                                                                                          <  2:5013/150‑1 (MQ=255)
                                                                  tAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTc                                                                                     <  2:913034/150‑1 (MQ=255)
                                                                   aGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCg                                                                                    <  2:1081092/150‑1 (MQ=255)
                                                                    ccAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCga                                                                                   <  2:1274981/149‑1 (MQ=255)
                                                                      tggCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTCAAATAAGAAACCCGGTCGCATCCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCgaga                                                                                 <  2:1053645/149‑1 (MQ=255)
                                                                       ggCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGaa                                                                                >  1:441555/1‑150 (MQ=255)
                                                                       ggCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGaa                                                                                <  1:853720/150‑1 (MQ=255)
                                                                         cgcTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATc                                                                              <  1:1078477/150‑1 (MQ=255)
                                                                           cTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGc                                                                            >  1:619952/1‑150 (MQ=255)
                                                                              cATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATc                                                                         >  2:23093/1‑150 (MQ=255)
                                                                              cATGTGGCACCTGTTAAAGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGTTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTAAGAGAATCGCATc                                                                         >  1:276131/1‑150 (MQ=255)
                                                                               aTGTGGCACCTGTTAATGATAAATGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCCAAACCGGGTTCAGAAGTAACGGTGTAATTAGCCCAACGGACGTACCGCTTCGCGCACCCCTTTTTCGAGAATCGCATcc                                                                        <  2:82101/150‑1 (MQ=255)
                                                                                tgtgGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCa                                                                       >  1:930425/1‑150 (MQ=255)
                                                                                tgtgGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAg                                                                      >  1:996669/1‑150 (MQ=255)
                                                                                  tgGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAgg                                                                     >  1:1158130/1‑150 (MQ=255)
                                                                                   ggCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGt                                                                    >  2:154548/1‑150 (MQ=255)
                                                                                        cTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTa                                                               >  1:1485105/1‑150 (MQ=255)
                                                                                         tGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACTGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCAATAg                                                              >  1:98318/1‑150 (MQ=255)
                                                                                         tGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAg                                                              <  1:749732/150‑1 (MQ=255)
                                                                                             aaTGTTATAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAAGCGGTTTCAGAAGTAACGGTGTTATTAGCACATCGGACGTACAGCTACGCGCATCCAGTTTTCGAGAATCGCATCCAGGTCATTAGCacc                                                          >  2:1131008/1‑148 (MQ=255)
                                                                                              aTGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAAcc                                                         <  2:1465839/150‑1 (MQ=255)
                                                                                              aTGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAAcc                                                         >  1:1430679/1‑150 (MQ=255)
                                                                                              aTGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAAcc                                                         <  1:1284211/150‑1 (MQ=255)
                                                                                                gATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCCGGTCATTAGCAACCTg                                                       >  2:1395218/1‑150 (MQ=255)
                                                                                                   aaaGGCATATAGTATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTc                                                    <  1:373690/150‑1 (MQ=255)
                                                                                                   aaaGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCta                                                    >  2:896044/1‑149 (MQ=255)
                                                                                                   aaaGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTc                                                    <  2:198722/150‑1 (MQ=255)
                                                                                                    aaGGAATATAGGATGTTGTAACTAATATGGTGATATATGAATCCCGTTCGAAACAGGGTTCAGAAGTAACGGTGTTATTAGCACTACGTACGTACAGCTTCGTGCTTCGCTTTTTCGAGATTCGCATCCAGGTCTTTAGCTACGTGCTCg                                                   <  1:1107333/150‑1 (MQ=255)
                                                                                                      ggCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgac                                                 <  1:521681/150‑1 (MQ=255)
                                                                                                          tataGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgaccaga                                             <  2:1232136/150‑1 (MQ=255)
                                                                                                            taGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgaccagacc                                           >  1:1515711/1‑150 (MQ=255)
                                                                                                              ggATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGaccagaccag                                         <  2:1636637/150‑1 (MQ=255)
                                                                                                               gATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAgg                                        <  1:1021624/150‑1 (MQ=255)
                                                                                                               gATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGGGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAgg                                        <  1:193795/150‑1 (MQ=255)
                                                                                                                   ttgtAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACt                                    <  2:638047/150‑1 (MQ=255)
                                                                                                                   ttgtAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACt                                    >  2:465885/1‑150 (MQ=255)
                                                                                                                   ttgtAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACt                                    <  1:1718828/150‑1 (MQ=255)
                                                                                                                     gtAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTg                                  >  2:548076/1‑150 (MQ=255)
                                                                                                                      tAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGc                                 <  2:1376704/150‑1 (MQ=255)
                                                                                                                         cTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTTCTCGACCAGACCAGGCTCTTGCGTc                              >  1:377140/1‑150 (MQ=255)
                                                                                                                         cTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTc                              <  2:732990/150‑1 (MQ=255)
                                                                                                                           aaTATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAAACGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCCCGTGGGTCAg                            >  2:1129821/1‑150 (MQ=255)
                                                                                                                              atGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTc                         >  2:988696/1‑150 (MQ=255)
                                                                                                                               tGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTcc                        <  1:1396834/150‑1 (MQ=255)
                                                                                                                                  tGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGc                     >  2:563627/1‑150 (MQ=255)
                                                                                                                                   gAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCt                    >  1:429936/1‑150 (MQ=255)
                                                                                                                                         aGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGt              <  2:629171/150‑1 (MQ=255)
                                                                                                                                         aGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGt              <  2:398270/150‑1 (MQ=255)
                                                                                                                                         aGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGt              <  2:706988/150‑1 (MQ=255)
                                                                                                                                            aaaCCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTgg            <  2:984154/150‑1 (MQ=255)
                                                                                                                                             aaCCCGGTCGAAACCGGGTTCAGAAGTAACGGCGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGt           >  1:373418/1‑150 (MQ=255)
                                                                                                                                              aCCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTc          <  2:488454/150‑1 (MQ=255)
                                                                                                                                                ccGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCtt        >  1:621436/1‑150 (MQ=255)
                                                                                                                                                  ggTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCtttt      <  1:472808/150‑1 (MQ=255)
                                                                                                                                                  ggTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCtttt      <  1:392377/150‑1 (MQ=255)
                                                                                                                                                     cGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCACCCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCt   >  2:881985/1‑150 (MQ=255)
                                                                                                                                                      gAAACCGTGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTg  <  2:824026/150‑1 (MQ=255)
                                                                                                                                                      gAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTg  <  2:1419119/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTG  >  NC_000913/1609052‑1609350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: