Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,620,227 C→G 10.9% intergenic (+20/+11) marB → / ← eamA periplasmic mar operon regulator/cysteine and O‑acetyl‑L‑serine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,620,2270CG10.9% 271.9 / 22.4 92intergenic (+20/+11)marB/eamAperiplasmic mar operon regulator/cysteine and O‑acetyl‑L‑serine efflux system
Reads supporting (aligned to +/- strand):  ref base C (31/51);  new base G (3/7);  total (34/58)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

TTCTTGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCC  >  NC_000913/1620078‑1620375
                                                                                                                                                     |                                                                                                                                                     
ttcttGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACAtc                                                                                                                                                       <  2:439729/150‑1 (MQ=255)
ttcttGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGAAGTTTGTTCTGGCCCCGACAtc                                                                                                                                                       >  2:993182/1‑150 (MQ=255)
  cttGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTACATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACAtctc                                                                                                                                                     <  1:625060/150‑1 (MQ=255)
  cttGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACAtctc                                                                                                                                                     <  1:383547/150‑1 (MQ=255)
     gtgCCAATGTCGTGGTTGTTCCGCCATCGCTGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCggg                                                                                                                                                  <  1:119857/150‑1 (MQ=255)
     gtgCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACACTATGTAGTTTGTTCTGGCCCCGACATCTCggg                                                                                                                                                  <  2:734142/150‑1 (MQ=255)
      tgCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCgggg                                                                                                                                                 <  1:1433241/150‑1 (MQ=255)
       gCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGc                                                                                                                                                <  1:1145240/150‑1 (MQ=255)
        ccAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGcc                                                                                                                                               <  2:1405262/150‑2 (MQ=255)
         cAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCtt                                                                                                                                              >  1:1014017/1‑150 (MQ=255)
          aaTGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGccag                                                                                                                                             <  2:1363761/150‑4 (MQ=255)
          aaTGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGccag                                                                                                                                             <  1:1431830/150‑4 (MQ=255)
          aaTGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGccag                                                                                                                                             <  2:1357102/150‑4 (MQ=255)
          aaTGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGccag                                                                                                                                             <  1:705283/150‑4 (MQ=255)
          aaTGGCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTAAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGccag                                                                                                                                             <  1:1287964/150‑4 (MQ=255)
           aTGTCGTGGTTGTTGCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGTGATGTCGGGGccaga                                                                                                                                            <  2:281812/150‑5 (MQ=255)
            tGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGccagaa                                                                                                                                           <  1:1350842/150‑6 (MQ=255)
               cGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGGCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAAc                                                                                                                                        <  2:321929/150‑1 (MQ=255)
                gTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACt                                                                                                                                       <  1:525665/150‑1 (MQ=255)
                 tGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACtt                                                                                                                                      <  2:438221/150‑1 (MQ=255)
                 tGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCGCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACtt                                                                                                                                      <  2:372199/150‑1 (MQ=255)
                  ggTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTc                                                                                                                                     >  1:248174/1‑150 (MQ=255)
                   gttgttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTcc                                                                                                                                    <  1:891507/150‑1 (MQ=255)
                    ttgttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTccc                                                                                                                                   <  1:202023/150‑1 (MQ=255)
                      gttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCACCACGCTATGTAGTGTGTTCTGGCCCGGACATCGCGGGGCTTATTAACTTCCCAc                                                                                                                                 >  2:1129984/1‑150 (MQ=255)
                         cccccATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCtt                                                                                                                              >  1:740520/1‑150 (MQ=255)
                          ccccATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCttt                                                                                                                             >  1:625718/1‑150 (MQ=255)
                           cccATCGCAGGAACACCCACCGTTTGATTTAAATCACATGAGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTa                                                                                                                            <  2:220053/150‑1 (MQ=255)
                            ccATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTAc                                                                                                                           <  2:1558885/150‑1 (MQ=255)
                            ccATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTAc                                                                                                                           <  1:1588286/150‑1 (MQ=255)
                             cATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTAcc                                                                                                                          >  2:646946/1‑150 (MQ=255)
                              aTCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCTGCGTGCCCTATTATAATCAACACGATATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCg                                                                                                                         >  1:77990/1‑150 (MQ=255)
                               tCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGc                                                                                                                        <  1:539936/150‑1 (MQ=255)
                                cGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTAGGTAGTGTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCt                                                                                                                       >  2:825386/1‑150 (MQ=255)
                                 gCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCtt                                                                                                                      >  1:740508/1‑150 (MQ=255)
                                    ggAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTAc                                                                                                                   <  1:1410110/150‑1 (MQ=255)
                                        cacccaccGTTTGATTTTAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGccc                                                                                                               >  1:315648/1‑149 (MQ=255)
                                        cacccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCa                                                                                                               >  1:1398444/1‑150 (MQ=255)
                                           ccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCg                                                                                                            <  1:1278648/150‑1 (MQ=255)
                                            caccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGc                                                                                                           >  2:818295/1‑150 (MQ=255)
                                            caccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGc                                                                                                           <  1:893440/150‑1 (MQ=255)
                                                gTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGc                                                                                                       <  1:818295/150‑1 (MQ=255)
                                                   tGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCGTAATATAATCCACACGCTATGGAGTTTGTTCTGGCCCCGACAGCTCGGGTAGTATTACCTTGACACCTTTAACGCTTAACGCCACCGCAAGCCaa                                                                                                    >  1:1165290/1‑150 (MQ=255)
                                                    gATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTACCTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCaaa                                                                                                   >  2:193959/1‑150 (MQ=255)
                                                     aTTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAAt                                                                                                  <  1:1457114/150‑1 (MQ=255)
                                                           aTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTg                                                                                            <  2:730613/150‑1 (MQ=255)
                                                           aTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTg                                                                                            <  2:35679/150‑1 (MQ=255)
                                                            tCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGa                                                                                           <  2:908761/150‑1 (MQ=255)
                                                               caTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGatat                                                                                        <  2:1365714/150‑1 (MQ=255)
                                                                   ggTACTGGCAGTGTTAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAg                                                                                   <  2:1223421/150‑1 (MQ=255)
                                                                    gTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTTTTATAATCAACACGCTATGTAGATTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCGACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGTTATACAGc                                                                                  <  2:821908/150‑1 (MQ=255)
                                                                      acaggCAGTGATAAGTCGGATGCGCTCGGTGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGccc                                                                                <  2:702525/147‑1 (MQ=255)
                                                                            aGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCAt                                                                          <  2:1635184/150‑1 (MQ=255)
                                                                             gTGATAAGTCGGATGCGCTCGGCGTGGCCTATTATAATCAACACGCTATGTTGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACAGCAAGCCAAATACATTGATATACAGCCCGGTCATa                                                                         <  2:1076295/150‑1 (MQ=255)
                                                                             gTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCCAGCCAAATTCATTGATATACTGCCAGGTCATa                                                                         >  1:259820/1‑150 (MQ=255)
                                                                               gATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAAt                                                                       >  1:504591/1‑150 (MQ=255)
                                                                                cttaggcGGATGCGCTCTGCGAGCCCTATTATAATAAACACTCTATTTAGTTTGTTCTGGCCACGAAATCTCGGTGATTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATTCATTGATATACAGCCCGGTCATAATg                                                                      <  1:288725/144‑1 (MQ=255)
                                                                                aTAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATg                                                                      <  2:1235971/150‑1 (MQ=255)
                                                                                aTAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCCACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATg                                                                      >  2:1569845/1‑150 (MQ=255)
                                                                                aTAAGTCGGATGAGCTCGGCGTGCAATATTATAATCAACAAGCTATGTAGTTTGTTATGGCAACGACATCTATGTGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATg                                                                      <  1:1157746/150‑1 (MQ=255)
                                                                                 tAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCGCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGa                                                                     >  1:1638306/1‑150 (MQ=255)
                                                                                  aaGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAg                                                                    <  2:1014017/150‑1 (MQ=255)
                                                                                   aGTCGGATGCGCTCGGCGTGCCCTATTATATTCAACAGGATATGTAGTTTGTTATGCCCCCGACTTCTCGGGGCTTATTAACTTCCCCCCTTTCCCGCTTTACGCCACCGCAAGCCAAATACTTTATTTACAACCCCGGGCATAATGAgc                                                                   >  2:648541/1‑150 (MQ=255)
                                                                                   aGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgc                                                                   <  1:1686338/150‑1 (MQ=255)
                                                                                      cGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACATCCCGGTCATAATGAgcacc                                                                >  2:1363575/1‑150 (MQ=255)
                                                                                      cGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcacc                                                                <  1:927869/150‑1 (MQ=255)
                                                                                            cgcTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCt                                                          <  2:1398069/150‑1 (MQ=255)
                                                                                            cgcTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCt                                                          >  1:911598/1‑150 (MQ=255)
                                                                                                  gCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAAt                                                    >  1:637318/1‑150 (MQ=255)
                                                                                                      gCCCTATTATAATCACCACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCTCCGCACCTAAAAATTGCa                                                >  1:1721712/1‑150 (MQ=255)
                                                                                                        ccTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGa                                              <  1:1367351/150‑1 (MQ=255)
                                                                                                           gttatAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTAAGCCACCGCAAGCCAAATACATTGATATACAGCCCGGGCATAATGAGCACCGCACCTAAAAATTGCAGAccc                                           >  1:1198395/2‑150 (MQ=255)
                                                                                                              ataATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGtt                                        <  2:1194377/150‑1 (MQ=255)
                                                                                                               taATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGTCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTa                                       >  2:1363597/1‑150 (MQ=255)
                                                                                                                aaTCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGGTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGCCCCGTTaa                                      >  2:1246747/1‑150 (MQ=255)
                                                                                                                   cAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATAAACAGCCCGGTCATAATGCGCACCGCACCTAATAATTGCAGACCCGTTAAGCg                                   >  2:227275/1‑150 (MQ=255)
                                                                                                                     acacGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGtt                                 <  1:33800/150‑1 (MQ=255)
                                                                                                                     acacGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGtt                                 <  1:917242/150‑1 (MQ=255)
                                                                                                                      cacGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTc                                <  2:740520/150‑1 (MQ=255)
                                                                                                                       acGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTGAAGCGTTCa                               >  2:881227/1‑150 (MQ=255)
                                                                                                                         gCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATc                             <  2:911598/150‑1 (MQ=255)
                                                                                                                          cTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATcc                            <  2:1398444/150‑1 (MQ=255)
                                                                                                                          cTATGTAGTTTGTTATGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCTCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATcc                            <  2:315648/150‑1 (MQ=255)
                                                                                                                           tATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCca                           <  2:1157492/150‑1 (MQ=255)
                                                                                                                             tGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTCTACGCCACCGCAATCGAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGCCCCGGTTAGCGGTCATCcaac                         >  2:295349/1‑150 (MQ=255)
                                                                                                                              gTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCcaaca                        >  1:588882/1‑150 (MQ=255)
                                                                                                                              gTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCcaaca                        <  1:1122343/150‑1 (MQ=255)
                                                                                                                                  tttGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAg                    <  2:637318/150‑1 (MQ=255)
                                                                                                                                     taataagCCCCGAGATGTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCCCCGCCAGCCAAATACATTGAGATAGAGCCCGGGCATAAGGAGCACCGCACCTAAAAATTGCAGACCCGTAAAGCGTTCATCCACCAATAGTGc                 >  2:842219/7‑150 (MQ=255)
                                                                                                                                     taataagCCCCGAGATGTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAATTTGCAGACCCGTTAAGCGTTCATCCAACAATAGTga                 >  2:315984/7‑149 (MQ=255)
                                                                                                                                     gTTCTGGCCCCGACATCTCTGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGAACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGc                 <  1:324027/150‑1 (MQ=255)
                                                                                                                                      aataagCCCCGAGATGTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGGCATAATGAGCACCGCACCAAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGcc                >  2:665545/6‑150 (MQ=255)
                                                                                                                                           gCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACt           <  1:1037455/150‑1 (MQ=255)
                                                                                                                                              ccGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGTCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGc        <  1:1363597/150‑1 (MQ=255)
                                                                                                                                               cGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGcc       <  1:1523515/150‑1 (MQ=255)
                                                                                                                                               cGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGcc       >  2:308329/1‑150 (MQ=255)
                                                                                                                                                    tctcGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTcc  <  2:511541/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCTTGTGCCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCCGTTAAGCGTTCATCCAACAATAGTGCCGCACTTGCCAGTCC  >  NC_000913/1620078‑1620375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: