Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,629,435 G→A 7.0% intergenic (+17/+18) ydfZ → / ← ydfI selenoprotein, function unknown/putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,629,4350GA 7.0% 303.3 / 8.2 100intergenic (+17/+18)ydfZ/ydfIselenoprotein, function unknown/putative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (53/40);  new base A (2/5);  total (55/45)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-01

Only 100 of 108 total aligned reads displayed.

CACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAG  >  NC_000913/1629286‑1629584
                                                                                                                                                     |                                                                                                                                                     
cacTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGccgccg                                                                                                                                                       <  2:1205731/150‑1 (MQ=255)
   tGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATg                                                                                                                                                    >  2:938830/1‑150 (MQ=255)
    ggCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACGTGATTCGTCTCGGCATGATCTATGGGTGTGAATGCCGCCGAggg                                                                                                                                                   >  1:57761/1‑150 (MQ=255)
    ggCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATgg                                                                                                                                                   >  2:101588/1‑150 (MQ=255)
        aGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATggcggc                                                                                                                                               <  1:1141798/150‑1 (MQ=255)
        aGATCCTGTCGATTGATACTGAAGGGCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATggcggc                                                                                                                                               <  2:1142020/150‑1 (MQ=255)
           tCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCATCGGCGGCAtt                                                                                                                                            <  1:1460051/150‑1 (MQ=255)
            ccTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGGCTCGGCATGAACTAAGCGTGTGAATGCCGCCATCGGCGGCAttc                                                                                                                                           <  2:102733/150‑2 (MQ=255)
            ccTGTCGATTGACACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTg                                                                                                                                           <  1:949129/150‑1 (MQ=255)
             cTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCATCGGCGGCAttca                                                                                                                                          <  1:1351615/150‑3 (MQ=255)
              tGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACATGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCATCGGCGGCAttcac                                                                                                                                         <  2:852201/150‑4 (MQ=255)
               gTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCATCGGCGGCAttcaca                                                                                                                                        <  1:1463667/150‑5 (MQ=255)
                  gATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAAGTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCttttt                                                                                                                                     <  2:134748/150‑1 (MQ=255)
                  gATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTAGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCGTGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCttttt                                                                                                                                     >  1:732689/1‑150 (MQ=255)
                   aTTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCTGAAAAACAGTAGTAGTTGAAGGTTGTGAAGAGAACCTGGCACCACTGGACATGATTCGTCTAGTCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTTCtttttt                                                                                                                                    <  1:710872/150‑1 (MQ=255)
                    ttGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTa                                                                                                                                   >  1:602142/1‑150 (MQ=255)
                        tACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTc                                                                                                                               >  1:371702/1‑150 (MQ=255)
                        tACTGAAGGTCTGACCGCTGAGCAAATACGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCAGTGCTTTTTTACTTc                                                                                                                               >  1:377898/1‑150 (MQ=255)
                         aCTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCa                                                                                                                              <  1:645198/150‑1 (MQ=255)
                          cTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGCTGGCGGCATTGCTTTTTTACTTCAc                                                                                                                             >  1:869906/1‑150 (MQ=255)
                          cTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCAc                                                                                                                             >  2:1373211/1‑150 (MQ=255)
                          cTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGGTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCAc                                                                                                                             <  1:1199453/150‑1 (MQ=255)
                            gAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACgg                                                                                                                           >  2:1268276/1‑150 (MQ=255)
                            gAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACgg                                                                                                                           >  1:913958/1‑150 (MQ=255)
                            gAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGGTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACgg                                                                                                                           <  1:105043/150‑1 (MQ=255)
                                  cTGCCCGCAGCGGAAATGCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGTCCTGATTAGTATCCGCATGAACTAAGCGTGTTAATGCAGCCGATGGCGGCATTGCTTGTTTAATTCCCGGAATAtt                                                                                                                     >  2:439235/1‑150 (MQ=255)
                                     aCCGCTGAGCACATCCTGCGCGGAAACACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAAATAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTg                                                                                                                  <  2:868567/150‑1 (MQ=255)
                                      ccGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGc                                                                                                                 >  1:359197/1‑150 (MQ=255)
                                      ccGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGc                                                                                                                 <  2:1221425/150‑1 (MQ=255)
                                      ccGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGc                                                                                                                 <  1:520579/150‑1 (MQ=255)
                                       cGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGcc                                                                                                                >  1:1675573/1‑150 (MQ=255)
                                       cGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGcc                                                                                                                <  1:405425/150‑1 (MQ=255)
                                          agAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGAATCGTCTCGGCATGAACTAAGTGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACg                                                                                                             >  2:1262913/2‑150 (MQ=255)
                                              cAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCg                                                                                                         <  2:1481904/150‑1 (MQ=255)
                                                aaTCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCt                                                                                                       >  2:79932/1‑150 (MQ=255)
                                                  tCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCttt                                                                                                     >  2:1294568/1‑150 (MQ=255)
                                                   ccgccgCGGAAAAACCGTAGTTGTTGACGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTc                                                                                                    <  1:572652/150‑1 (MQ=255)
                                                   ccgccgCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTc                                                                                                    <  1:565509/150‑1 (MQ=255)
                                                    cgccgcGGAAAAACCGTAGTTGTTGAATGTTGTGAAGAGAAACTGTCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTcg                                                                                                   <  1:1032664/150‑1 (MQ=255)
                                                    cgccgcGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTcg                                                                                                   <  1:1330671/150‑1 (MQ=255)
                                                        gcgGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGcc                                                                                               >  2:878315/1‑150 (MQ=255)
                                                           gAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATg                                                                                            >  1:1389261/1‑150 (MQ=255)
                                                               aaCCGTAGTTGTTGCAGGTTGTGAAGCGAACCTGGCACCACTGGACCTGAGTCGGCTCGGCATGTACTAAGCGTGTGTAAGCCGCCGATGGCGGCATTGCTTTTTTACTTCACTGAATATTTTGCCACGGTCGCATTCGCGCCATGCGCt                                                                                        <  1:1092140/150‑1 (MQ=255)
                                                               aaCCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGCTGGCGGCAATGCTTTTTTACTACACGGAATATTTTGCCACTGTCGCTTTCGCGCCATGCGCt                                                                                        >  1:911332/1‑150 (MQ=255)
                                                                aCCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCta                                                                                       <  2:1017995/150‑1 (MQ=255)
                                                                  cGTAGTTGTTGCAGGTTGGGAAGAGTAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTTAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCtaat                                                                                     <  1:1176608/150‑1 (MQ=255)
                                                                   gTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCtaata                                                                                    <  2:486524/150‑1 (MQ=255)
                                                                    tAGTTGTTGAAGGTTGTGAAGAGAAAATGGCACCACTGGACCTGATTCGTCTGGTCATGTACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACTGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCtaataa                                                                                   <  2:1172003/150‑1 (MQ=255)
                                                                        tgttgAAGGTTGTGAAGAGAAACTGGCCCCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGAc                                                                               >  2:498328/1‑150 (MQ=255)
                                                                           tgAAGGTTGTGAAGAGAAACTGGCACCACTGGCCCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAg                                                                            >  1:168144/1‑150 (MQ=255)
                                                                           tgAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAg                                                                            <  1:142920/150‑1 (MQ=255)
                                                                           tgAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAg                                                                            <  1:1522365/150‑1 (MQ=255)
                                                                           tgAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAg                                                                            >  1:1615850/1‑150 (MQ=255)
                                                                              aGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTAc                                                                         >  2:1345617/1‑150 (MQ=255)
                                                                                      aaGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGt                                                                 <  2:913958/150‑1 (MQ=255)
                                                                                      aaGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGt                                                                 >  2:922030/1‑150 (MQ=255)
                                                                                       agagAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTc                                                                >  2:1156741/1‑150 (MQ=255)
                                                                                       agagAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTc                                                                <  1:88363/150‑1 (MQ=255)
                                                                                       agagAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTc                                                                >  1:524579/1‑150 (MQ=255)
                                                                                        gagaAACTGGATCCACTGGACCTGATTCGTCTCGGCATCAACTAAGCGTGTGATTGCCGCCGATGGCGGCATTGCTTTTTTACTTCTCGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCa                                                               <  1:1159256/150‑1 (MQ=255)
                                                                                         agaAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCTTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCAc                                                              <  1:931089/150‑1 (MQ=255)
                                                                                          gaAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCCCGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCAct                                                             >  2:72085/1‑150 (MQ=255)
                                                                                          gaAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCAct                                                             >  1:1581277/1‑150 (MQ=255)
                                                                                             aCTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCtt                                                          >  2:730297/1‑150 (MQ=255)
                                                                                                ggCACCTCTGGACATGATTCGTCTCGGCGTGAACTAAGCGTGGTTATGCATACGATTGCGGGATTGCTTTTTTACTTGACGGAATATTTTGCAACGGTCGCTTTCGCTACATGCGCTAATAAAGACAAGTACTTTTCCGTCACTCTTGCa                                                       <  2:732689/150‑1 (MQ=255)
                                                                                                 gCACCACTGGACCTGATTCTGCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAg                                                      <  2:347750/150‑1 (MQ=255)
                                                                                                 gCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAg                                                      <  1:938830/150‑1 (MQ=255)
                                                                                                  caccacTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGt                                                     >  1:631755/1‑150 (MQ=255)
                                                                                                    ccacTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaa                                                   >  1:716335/1‑150 (MQ=255)
                                                                                                    ccacTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaa                                                   <  2:410157/150‑1 (MQ=255)
                                                                                                         ggACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaacaaa                                              >  1:901868/1‑150 (MQ=255)
                                                                                                         ggACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaacaaa                                              >  1:423761/1‑150 (MQ=255)
                                                                                                          gACCTGATTCGTCTCGGCATGAACTAAGCTTGTGAATGCCGCCGATGGAGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaacaaac                                             <  2:1504615/150‑1 (MQ=255)
                                                                                                            ccTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTa                                           >  1:1190643/1‑150 (MQ=255)
                                                                                                               gATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTg                                        <  2:602142/150‑1 (MQ=255)
                                                                                                                  tCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCt                                     <  1:922030/150‑1 (MQ=255)
                                                                                                                   cGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCCCTCTTGCCGTAAACAAACTATTGTCTg                                    >  2:1159882/1‑150 (MQ=255)
                                                                                                                   cGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTGGCCACGGTCGCTTTCGCGCCAGTGGCTAATAAAGCCAAGTACGTTTCCGTCACTCTGGCTGTAAACCAACTATTGGCTg                                    >  2:812679/1‑150 (MQ=255)
                                                                                                                      ctcGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGAATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCa                                 >  2:112452/1‑150 (MQ=255)
                                                                                                                         ggCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAat                              >  2:755554/1‑150 (MQ=255)
                                                                                                                          gCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAatc                             >  1:504167/1‑150 (MQ=255)
                                                                                                                           cATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAatca                            <  2:1615850/150‑1 (MQ=255)
                                                                                                                           cATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAatca                            <  2:168144/150‑1 (MQ=255)
                                                                                                                           cATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAatca                            <  2:1379573/150‑1 (MQ=255)
                                                                                                                           cATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAatca                            >  1:1508395/1‑150 (MQ=255)
                                                                                                                                aCTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCAcc                       >  2:477546/1‑150 (MQ=255)
                                                                                                                                aCTAAGCGTGTGAATGCCGCCGATGGCGGCATTCCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCTCCATCCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCAcc                       >  2:1048203/1‑150 (MQ=255)
                                                                                                                                  tAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCaa                     <  2:1436336/150‑1 (MQ=255)
                                                                                                                                         gtgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTTATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGc              >  2:1097196/1‑150 (MQ=255)
                                                                                                                                         agcaaTGCCGCCATCGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGc              >  2:297335/4‑150 (MQ=255)
                                                                                                                                           gAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCt            >  2:1381787/1‑150 (MQ=255)
                                                                                                                                           caaTGCCGCCATCGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAACCTATTGTCTGGCAAATCATCACCAAAGATCGCCt            >  2:1187729/2‑150 (MQ=255)
                                                                                                                                               gccgccGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAAt        >  1:262953/1‑150 (MQ=255)
                                                                                                                                                ccgccgATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATc       >  1:529385/1‑150 (MQ=255)
                                                                                                                                                ccgccgATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATc       <  1:1345617/150‑1 (MQ=255)
                                                                                                                                                 cgccgATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCg      <  1:968672/150‑1 (MQ=255)
                                                                                                                                                 cgccgATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCg      <  1:190779/150‑1 (MQ=255)
                                                                                                                                                  gccgATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGc     >  1:1151340/1‑150 (MQ=255)
                                                                                                                                                   ccgATGGCGGCATTGCGTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGcc    >  1:1397620/1‑150 (MQ=255)
                                                                                                                                                     gATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAg  <  1:101588/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAG  >  NC_000913/1629286‑1629584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: