Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,640,497 T→C 5.2% K30R (AAA→AGA) ‡ essQ ← Qin prophage; putative S lysis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,640,4970TC 5.2% 351.8 / 6.8 115K30R (AAA→AGA) ‡essQQin prophage; putative S lysis protein
Reads supporting (aligned to +/- strand):  ref base T (49/60);  new base C (3/3);  total (52/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01

Only 100 of 116 total aligned reads displayed.

CATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGTTTGAAAAGATCGGAGTCTACGGGGT  >  NC_000913/1640350‑1640646
                                                                                                                                                   |                                                                                                                                                     
cATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTa                                                                                                                                                     >  1:612144/1‑150 (MQ=255)
  tAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATc                                                                                                                                                   >  1:150242/1‑150 (MQ=255)
   aGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCg                                                                                                                                                  <  2:1422690/150‑1 (MQ=255)
   aGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCg                                                                                                                                                  <  1:1454327/150‑1 (MQ=255)
   aGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCg                                                                                                                                                  >  1:1253783/1‑150 (MQ=255)
            tCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGc                                                                                                                                         <  2:1001120/150‑1 (MQ=255)
            tCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGc                                                                                                                                         >  1:108736/1‑150 (MQ=255)
             cATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCCCTGTGACGGAGTTACTTTATCGAGTAACTGCa                                                                                                                                        >  1:1456752/1‑150 (MQ=255)
             cATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCa                                                                                                                                        >  2:1034120/1‑150 (MQ=255)
             cATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACAACGATTGCAGCCCACTGTGACGGAGTTACTTTTTCGAGTAACTGCa                                                                                                                                        >  1:457486/1‑150 (MQ=255)
              aTTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCaa                                                                                                                                       >  1:1091824/1‑150 (MQ=255)
              aTTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGCAGTTACTCGATAAAGTAACtccgt                                                                                                                                       <  1:1204891/150‑5 (MQ=18)
               ttGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTCGATAAAGTAACtccgtc                                                                                                                                      <  1:1560581/150‑6 (MQ=18)
               ttGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGCAGTTACTCGATAAAGTAACtccgtc                                                                                                                                      <  2:347809/150‑6 (MQ=18)
                 ggATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTTATCTTGATATAAAGTTTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACCCCGATTGCAGCCCACTGTGACGGAGTTACATTTTCGAGGATCTGCAATGc                                                                                                                                    >  2:158264/1‑150 (MQ=255)
                     tACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCTGGCCAAAAACCAGGCTACCCAGCACTCCGATTGCAGCCCACTGTTACGGAGTTACTTTATCGAGTAACTGCAATGCGCAg                                                                                                                                <  1:312277/150‑1 (MQ=255)
                     tACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAg                                                                                                                                <  1:1113492/150‑1 (MQ=255)
                      aCTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGa                                                                                                                               >  1:154176/1‑150 (MQ=255)
                       ctctcctctcGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAACAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTGACTTTATCGAGGTACTGCAATGCCCAGaa                                                                                                                              >  2:103718/1‑150 (MQ=255)
                       ctctcctctcGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGaa                                                                                                                              >  2:1224870/1‑150 (MQ=255)
                       ctctcctctcGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGaa                                                                                                                              >  2:1552802/1‑150 (MQ=255)
                       cgctcctctcGCAGCCTTTCGCCTGTGGTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCCGCAGGCCAAAAACCAGGCGACCGAGCACCCCGATTGCAGGGCCCTGTGACGGAGTAACTTTATCGAGTAACTGCACTGCCCAGac                                                                                                                              >  2:378978/3‑149 (MQ=18)
                        tctcctctcGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGaaa                                                                                                                             <  2:1546725/150‑1 (MQ=255)
                        tctcctctcGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGaaa                                                                                                                             >  1:1238366/1‑150 (MQ=255)
                         ctcctctcGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAAc                                                                                                                            >  1:177381/1‑150 (MQ=255)
                           cctctcGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCa                                                                                                                          >  2:368272/1‑150 (MQ=255)
                           cctctcGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCa                                                                                                                          >  2:1147722/1‑150 (MQ=255)
                            ctctcGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAg                                                                                                                         <  2:621793/150‑1 (MQ=255)
                             tctcGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGc                                                                                                                        <  2:721625/150‑1 (MQ=255)
                              ctcGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCa                                                                                                                       <  1:951828/150‑1 (MQ=255)
                                    gCCTTACGCCTGTCTTCTTTAATGTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGGAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTAccc                                                                                                                 <  2:903679/150‑1 (MQ=255)
                                       ttACGCCTGTCTTCTTTAATCTTGTAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACccgcc                                                                                                              <  2:1228329/150‑1 (MQ=255)
                                           gCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCCGAAACCAGCATTACCCGCCGAtg                                                                                                          >  2:1653722/1‑150 (MQ=255)
                                             cTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGAtgtg                                                                                                        <  1:368272/150‑1 (MQ=255)
                                             cTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGAtgtg                                                                                                        <  1:1147722/150‑1 (MQ=255)
                                             cTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGAtgtg                                                                                                        <  2:64577/150‑1 (MQ=255)
                                             cTGTCTTATTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGAtgtg                                                                                                        <  1:706774/150‑1 (MQ=255)
                                               gTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGcc                                                                                                      >  2:698007/1‑150 (MQ=255)
                                               gTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGcc                                                                                                      <  1:875777/150‑1 (MQ=255)
                                                tcttctTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCa                                                                                                     <  2:657524/150‑1 (MQ=255)
                                                 cttcttTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCCCTGTGACGGAGTTACTTTATCGAGTAACTGCTATGCCCAGAAACCTGCATTACCCGCCGATGTGCCAt                                                                                                    >  2:250976/1‑150 (MQ=255)
                                                   tcttTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAg                                                                                                  >  1:1702192/1‑150 (MQ=255)
                                                      ttAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCa                                                                                               <  2:182027/150‑1 (MQ=255)
                                                        aaTCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAac                                                                                             <  2:154176/150‑1 (MQ=255)
                                                           catGAAATACAGAGTTGTCAGATACGTCAGCAGGCCAAACCCCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGGTACTTAATCGAGTAACTGCAAAGCCCAGAAACCAGCATTACCCGCCGATGTGCCAAAGGCAACAcc                                                                                          <  1:607648/148‑1 (MQ=255)
                                                           cTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACAcc                                                                                          <  2:150242/150‑1 (MQ=255)
                                                           cTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACAcc                                                                                          >  2:1716060/1‑150 (MQ=255)
                                                              gacaTAAAGATTTGTCAGATACGTCAGCAGGCCAACAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTAATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCtgt                                                                                       <  1:1629089/147‑1 (MQ=255)
                                                              gAAATAAAGATTTGACAGATACGTCAGCAGGCCAAAAACCAGGATACCCAGAACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCAGCCGATGTGCAATAGGCAACACCtgt                                                                                       >  1:994759/1‑150 (MQ=255)
                                                               aaaTAAAGATTTGTCAGATACGTCAGCAGGCCAAAACCCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTAATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCtgtt                                                                                      <  1:91634/150‑1 (MQ=255)
                                                                 aTAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCtgttgt                                                                                    >  1:1555500/1‑150 (MQ=255)
                                                                  tAAAGATTAGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTgttgtt                                                                                   <  2:1411142/150‑1 (MQ=255)
                                                                   aaaGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTa                                                                                  >  2:573934/1‑150 (MQ=255)
                                                                   aaaGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTa                                                                                  <  1:1376387/150‑1 (MQ=255)
                                                                      taTTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACt                                                                               <  2:787016/149‑1 (MQ=255)
                                                                      gATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACt                                                                               >  2:790933/1‑150 (MQ=255)
                                                                      gATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACt                                                                               <  1:496116/150‑1 (MQ=255)
                                                                      gATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACt                                                                               <  2:1733570/150‑1 (MQ=255)
                                                                      gATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACt                                                                               >  2:229214/1‑150 (MQ=255)
                                                                          tGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATc                                                                           >  1:673645/1‑150 (MQ=255)
                                                                           gTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATcc                                                                          <  2:1671089/150‑1 (MQ=255)
                                                                             cAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCACCACCTGTTGTTAACTTATACAt                                                                        >  2:1048799/1‑150 (MQ=255)
                                                                             cAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCAt                                                                        <  2:607384/150‑1 (MQ=255)
                                                                              aGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCAtt                                                                       <  1:935394/150‑1 (MQ=255)
                                                                               gATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTg                                                                      <  2:1253783/150‑1 (MQ=255)
                                                                               acgccGTCAGCAGGCCAAAAACCAGGATACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTg                                                                      <  1:825496/146‑1 (MQ=255)
                                                                                   cGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGAttt                                                                  <  1:847640/150‑1 (MQ=255)
                                                                                      cagcagGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCTATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCat                                                               >  2:871869/1‑150 (MQ=255)
                                                                                      cagcagGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCat                                                               <  1:492712/150‑1 (MQ=255)
                                                                                       agcagGCCAAAAACCAGTCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTATCTGCAATTCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCata                                                              <  1:516963/150‑1 (MQ=255)
                                                                                       agcagGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCata                                                              <  1:1224870/150‑1 (MQ=255)
                                                                                       agcagGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCata                                                              <  1:1552802/150‑1 (MQ=255)
                                                                                       agcagGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCata                                                              <  1:492270/150‑1 (MQ=255)
                                                                                        gcagGCGAAAAACAAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCatat                                                             <  2:1247701/150‑1 (MQ=255)
                                                                                        gcagGCCAAAAACCAGGCTACCCAGCACCCCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGACACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCatat                                                             >  1:710113/1‑150 (MQ=255)
                                                                                        gcagGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCAATTGATTTCatat                                                             >  1:860564/1‑150 (MQ=255)
                                                                                         cagGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATTGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATc                                                            >  2:670180/1‑150 (MQ=255)
                                                                                             ccAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCa                                                        >  1:542820/1‑150 (MQ=255)
                                                                                              cAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCAc                                                       >  1:1602716/1‑150 (MQ=255)
                                                                                                    ccAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCACCACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGAt                                                 >  2:127215/1‑150 (MQ=255)
                                                                                                    ccAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTAATTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTGCATATCCTCACCCCGAt                                                 >  1:73277/1‑150 (MQ=255)
                                                                                                      aGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGt                                               <  2:108736/150‑1 (MQ=255)
                                                                                                           aCCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACg                                          <  1:959791/150‑1 (MQ=255)
                                                                                                            cccAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTACCTTATCCATTGATTTCATATCCTCACCCCGATGTACACgg                                         >  2:1017235/1‑150 (MQ=255)
                                                                                                                 cacaCCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGt                                    >  2:1244974/1‑150 (MQ=255)
                                                                                                                 cacaCCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGt                                    <  2:1091824/150‑1 (MQ=255)
                                                                                                                   cacCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCTGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGc                                  <  2:287543/150‑1 (MQ=255)
                                                                                                                            cAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGttt                         >  2:1018287/1‑150 (MQ=255)
                                                                                                                             aGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGTTTg                        <  1:1048728/150‑1 (MQ=255)
                                                                                                                             aGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGTTTg                        <  2:1650776/150‑1 (MQ=255)
                                                                                                                              tcccACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGTTTGa                       >  1:1069577/2‑150 (MQ=255)
                                                                                                                              gCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGTTTGa                       >  2:1061782/1‑150 (MQ=255)
                                                                                                                                 cACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGTTTGaaaa                    <  1:669572/150‑1 (MQ=255)
                                                                                                                                     gtgACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGTTTGAAAAGATc                <  1:1034120/150‑1 (MQ=255)
                                                                                                                                      tgACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGTTTGAAAAGATCg               <  1:546121/150‑1 (MQ=255)
                                                                                                                                       gACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATCACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGTTTGAAAAGATCgg              <  2:177381/150‑1 (MQ=255)
                                                                                                                                         tgcaGTTACTCGATAAAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGTTTGAAAAGATCGGAg            >  2:1561486/4‑150 (MQ=255)
                                                                                                                                          gcaGTTACTCGATAAAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGTTTGAAAAGATCGGAGt           >  1:1218600/3‑150 (MQ=255)
                                                                                                                                          gcaGTTACTCGATAAAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGTTTGAAAAGATCGGAGt           >  1:730817/3‑150 (MQ=255)
                                                                                                                                                   ttATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGTTTGAAAAGATCGGAGTCTACGGGGt  >  1:1704277/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CATAGTCTTGAGTCATTGGATTACTCTCCTCTCGCAGCCTTACGCCTGTCTTCTTTAATCTTGAAATAAAGATTTGTCAGATACGTCAGCAGGCCAAAAACCAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAATATGTTTGAAAAGATCGGAGTCTACGGGGT  >  NC_000913/1640350‑1640646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: