Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,660,438 | A→G | 6.6% | S790S (TCA→TCG) | ynfE → | putative selenate reductase, periplasmic |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,660,438 | 0 | A | G | 6.6% | 295.2 / 9.8 | 107 | S790S (TCA→TCG) | ynfE | putative selenate reductase, periplasmic |
Reads supporting (aligned to +/- strand): ref base A (49/48); new base G (2/5); total (54/53) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.37e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-01 |
Only 100 of 113 total aligned reads displayed.
GGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGA > NC_000913/1660289‑1660586 | ggCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTca > 1:1372461/1‑150 (MQ=255) gCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTcac > 1:1501067/1‑150 (MQ=255) gCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTcac < 1:317049/150‑1 (MQ=255) aGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGCGCCTGGCACGAGGCCAATATGTCTGGCGATACAATCGACCATGGCGGCTGTGTGAATACGCGAACAACTCTGCGACCTTCACCACt > 1:1523605/1‑150 (MQ=255) gTTCGGCTACCAGCCAAAGTATCACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCCCCACTg > 2:313303/1‑150 (MQ=255) ggCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGa < 2:1209223/150‑1 (MQ=255) cTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑g > 2:1619887/1‑150 (MQ=255) tACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑gg < 1:1048587/150‑1 (MQ=255) aacAGCCAAAGTAACACCACGTTTTCTCCCTGGAGTTAGCGCTATGGTCCAGGGAGCCTGGCAGGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCTCTCTGCGCCCTTCACCACTGGCGAA‑ggg < 2:1429500/148‑1 (MQ=255) aCCATCCAAAGTCCCACCACGTATTATCCCTGGAGTTCGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCATTATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑ggg < 1:250888/150‑1 (MQ=255) aCCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑ggg > 1:1316581/1‑150 (MQ=255) ccAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAA‑gggc < 2:1701739/150‑2 (MQ=255) ccAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAA‑gggc < 1:1041791/150‑2 (MQ=255) cAGCCAAAGTAACACGACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGGACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAA‑gggcg < 2:201649/150‑3 (MQ=255) aGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGaaa > 1:141352/1‑150 (MQ=255) ccAAAGTAACACCTCGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAAcc < 1:1191290/150‑1 (MQ=255) ccAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATACGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAccc > 1:1083119/1‑150 (MQ=255) cAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAAccc > 2:428204/1‑150 (MQ=255) aaGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCCCCACTGGCGAA‑GGGAAGCCCgc > 1:1266339/1‑150 (MQ=255) aGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCCCGAGGCCAATATGTCTGGCGCTAAAATCGACCATGGCTGCTGAGTGAATACTCTAACAACTCTGCGCCCATCACCACTGGCGAA‑GGTAAACCCgca > 2:311660/1‑150 (MQ=255) aGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCgca < 1:848921/150‑1 (MQ=255) aGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGCCTGTGTGAATACGCTACCCACTCTGCGCCCTTCCCCCCTGGCGAA‑GGGAAACCCgca > 2:502792/1‑150 (MQ=255) aaCACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCgcagcc > 1:1302442/1‑149 (MQ=255) acacCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGcac < 1:512612/150‑1 (MQ=255) cacGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTa > 1:568652/1‑150 (MQ=255) aTTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTg > 2:864608/1‑150 (MQ=255) ctcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTc < 1:1000729/150‑1 (MQ=255) ccTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGTCCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCgag < 1:39051/150‑1 (MQ=255) tGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGAt < 1:160779/150‑1 (MQ=255) ggAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAATATCGACGATGGCGGCTGTGTGAATACGCTTACCACTCTGCGCCCTTCACCGCTGGCGAA‑GGGATACCCGCAGCTCACTAATCTGGTCGAGATc > 1:1530084/1‑150 (MQ=255) gcgcTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa < 1:1077786/150‑1 (MQ=255) gcgcTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa > 2:317777/1‑150 (MQ=255) cTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACAACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAatat > 2:1012595/1‑150 (MQ=255) tATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAAtata < 2:933449/150‑1 (MQ=255) gggCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAattataa > 2:712917/1‑145 (MQ=255) gggCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAcc > 1:733237/1‑150 (MQ=255) gggCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAcc > 2:773931/1‑150 (MQ=255) ccAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCAc < 1:1432326/150‑1 (MQ=255) gggAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAACTATAACCCACGAc > 2:444779/1‑150 (MQ=255) aGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAc < 1:1709951/150‑1 (MQ=255) aGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAc > 1:61874/1‑150 (MQ=255) ccTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTACCCACTCTGCGCCCTTCACCCCTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGCCAACCa > 2:1581235/1‑150 (MQ=255) ccTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCa < 1:499269/150‑1 (MQ=255) ccTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCa > 1:551907/1‑150 (MQ=255) ccTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCT‑GTCGAGATCGAAAAAATATAACCCACGACAACCAt > 2:1510522/1‑150 (MQ=255) cACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAAt < 2:1079672/150‑1 (MQ=255) ggCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGCTCGAAAAAATATAACCCACGACAACCATAAATTCTgg > 2:690146/1‑150 (MQ=255) gCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGc < 2:1692740/150‑1 (MQ=255) ccAATATGTCTGGCGATAAATTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGTA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGTTCGAAAAATTATTTCCGACGACACCCTTAATTTCTGGCa > 2:250591/1‑150 (MQ=255) ccAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCCCTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCCCGACAACCATAAATTCTGGCa > 1:94611/1‑150 (MQ=255) ccAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTTGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCa < 1:297350/150‑1 (MQ=255) aaaTATGTCTGTCGATAATATCGACCATGTCGGATGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTTGCGAA‑GGGATACCCGCAGCACACTAATCTGGTCGAGATCGATAAAATATAACCCACGACAACCATAAATTCTGGCAt < 2:882945/149‑1 (MQ=255) aaTATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATg > 2:1513262/1‑150 (MQ=255) tGTCTGGCTATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAt < 1:169582/150‑1 (MQ=255) gTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAtt > 1:129481/1‑150 (MQ=255) gTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTAACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAtt > 2:564595/1‑150 (MQ=255) tCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAttt > 2:1700278/1‑150 (MQ=255) cTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAtttt < 2:733237/150‑1 (MQ=255) ggCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAATCCCGCAGCACACTAATCTGGTCGAGATCGAAAAACTATTTCCCACGACAACCTTAAATTCTGGCTTGACTGtttgt > 1:1049849/1‑150 (MQ=255) ggCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgt > 2:738515/1‑150 (MQ=255) ggCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgt > 2:771933/1‑150 (MQ=255) ggCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgt > 1:1023999/1‑150 (MQ=255) gCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtt < 1:1275143/150‑1 (MQ=255) cGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgttt < 2:737223/150‑1 (MQ=255) cGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCT‑GTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtttg < 1:1510522/150‑1 (MQ=255) gATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtttg < 2:233110/150‑1 (MQ=255) gATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtttg < 2:1654035/150‑1 (MQ=255) aTAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGa > 1:179814/1‑150 (MQ=255) tAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaa < 1:104886/150‑1 (MQ=255) tAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaa < 1:28339/150‑1 (MQ=255) aaaaTCGACCATGGCGTCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGGCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaaa < 1:238624/150‑1 (MQ=255) aTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGAAGCACACTAATCTGGTCTATATAGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGc < 2:1316581/150‑1 (MQ=255) gACCATGGCGGCTGTGTGAATACTCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCTAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAAt < 1:325112/150‑1 (MQ=255) tGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTg < 1:600859/150‑1 (MQ=255) tGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTg < 1:502792/150‑1 (MQ=255) ggcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGa > 2:1147632/1‑150 (MQ=255) ggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTa < 2:705131/150‑1 (MQ=255) gcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTaa > 1:1237471/1‑150 (MQ=255) gcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTaa > 1:418616/1‑150 (MQ=255) tgtgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAatg < 2:111012/150‑1 (MQ=255) tgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAAtgatga > 2:1545093/1‑150 (MQ=255) aTACGCTAACCACTCTGCGCCCTTCACCACTGGCGAC‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATACCCCACGACCACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGataa > 1:1166523/1‑150 (MQ=255) aCGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATc < 1:212233/150‑1 (MQ=255) cGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATcc < 2:28150/150‑1 (MQ=255) gCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCa < 2:852088/150‑1 (MQ=255) cTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCaa > 2:1306897/1‑149 (MQ=255) cTAACCACTCTGCGCCCTTCACCACTGGCGAA‑CGGAAACCAGCAGCAAACTAATCTTGTCTAGATCTAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCAt < 2:1133227/150‑1 (MQ=255) aaCCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc < 1:708868/150‑1 (MQ=255) aaCCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc < 2:962921/150‑1 (MQ=255) gggtttcccTTCGCCAGTGGTGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACaga > 2:525454/7‑150 (MQ=255) ctGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACagag < 1:565271/150‑1 (MQ=255) gcgcCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGgc > 1:1617341/1‑150 (MQ=255) tcccTTCGCCAGTGGTGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGgcgc > 2:1294689/2‑150 (MQ=255) cTTCACCACTGGCGAA‑GGGAAACCCGCCGCTCACTAATCTGGTCGAGATCGAAAAAATATAACCCACGCCAACCATAAATTCTGGCATGACATTTTGTTTGAATAGCAATAAGTGAGTAATGATGAAAATCCATACCCCAGAGGCGCTCa > 1:95639/1‑150 (MQ=255) cTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCa < 2:1061496/150‑1 (MQ=255) cTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCa > 1:1211438/1‑150 (MQ=255) ttCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCAt < 1:1669742/150‑1 (MQ=255) ttCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCAt < 2:916375/150‑1 (MQ=255) caccacTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATATGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGa > 2:927241/1‑150 (MQ=255) caccacTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGa > 2:1621841/1‑150 (MQ=255) | GGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAA‑GGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGA > NC_000913/1660289‑1660586 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |