Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,660,446 C→T 6.1% A793V (GCG→GTG)  ynfE → putative selenate reductase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,660,4460CT 6.1% 345.0 / 7.0 113A793V (GCG→GTG) ynfEputative selenate reductase, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (55/51);  new base T (2/5);  total (57/56)
Fisher's exact test for biased strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 113 total aligned reads displayed.

GGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCTGA  >  NC_000913/1660299‑1660593
                                                                                                                                                   |                                                                                                                                                    
ggCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGa                                                                                                                                                    <  2:1209223/150‑1 (MQ=255)
  cTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAg                                                                                                                                                 >  2:1619887/1‑150 (MQ=255)
   tACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAgg                                                                                                                                                <  1:1048587/150‑1 (MQ=255)
    aacAGCCAAAGTAACACCACGTTTTCTCCCTGGAGTTAGCGCTATGGTCCAGGGAGCCTGGCAGGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCTCTCTGCGCCCTTCACCACTGGCGAAggg                                                                                                                                               <  2:1429500/148‑1 (MQ=255)
    aCCATCCAAAGTCCCACCACGTATTATCCCTGGAGTTCGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCATTATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAggg                                                                                                                                               <  1:250888/150‑1 (MQ=255)
    aCCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAggg                                                                                                                                               >  1:1316581/1‑150 (MQ=255)
     ccAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAAgggc                                                                                                                                              <  1:1041791/150‑2 (MQ=255)
     ccAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAAgggc                                                                                                                                              <  2:1701739/150‑2 (MQ=255)
     ccAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAAgggc                                                                                                                                              <  2:28520/150‑2 (MQ=255)
      cAGCCAAAGTAACACGACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGGACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCGCCAGTGGTGAAgggcg                                                                                                                                             <  2:201649/150‑3 (MQ=255)
       aGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGaaa                                                                                                                                            >  1:141352/1‑150 (MQ=255)
         ccAAAGTAACACCTCGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAAcc                                                                                                                                          <  1:1191290/150‑1 (MQ=255)
         ccAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATACGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAccc                                                                                                                                          >  1:1083119/1‑150 (MQ=255)
          cAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAAccc                                                                                                                                         >  2:428204/1‑150 (MQ=255)
            aaGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCCCCACTGGCGAAGGGAAGCCCgc                                                                                                                                       >  1:1266339/1‑150 (MQ=255)
             aGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCCCGAGGCCAATATGTCTGGCGCTAAAATCGACCATGGCTGCTGAGTGAATACTCTAACAACTCTGCGCCCATCACCACTGGCGAAGGTAAACCCgca                                                                                                                                      >  2:311660/1‑150 (MQ=255)
             aGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgca                                                                                                                                      <  1:848921/150‑1 (MQ=255)
             aGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGCCTGTGTGAATACGCTACCCACTCTGCGCCCTTCCCCCCTGGCGAAGGGAAACCCgca                                                                                                                                      >  2:502792/1‑150 (MQ=255)
                aaCACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCgcagcc                                                                                                                                   >  1:1302442/1‑149 (MQ=255)
                 acacCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGcac                                                                                                                                  <  1:512612/150‑1 (MQ=255)
                  caccacGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGcaca                                                                                                                                 >  1:1572322/1‑150 (MQ=255)
                     cacGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTa                                                                                                                              >  1:568652/1‑150 (MQ=255)
                          aTTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTg                                                                                                                         >  2:864608/1‑150 (MQ=255)
                             ctcCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTc                                                                                                                      <  1:1000729/150‑1 (MQ=255)
                                ccTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGTCCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCgag                                                                                                                   <  1:39051/150‑1 (MQ=255)
                                   ggAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAATATCGACGATGGCGGCTGTGTGAATACGCTTACCACTCTGCGCCCTTCACCGCTGGCGAAGGGATACCCGCAGCTCACTAATCTGGTCGAGATc                                                                                                                >  1:1530084/1‑150 (MQ=255)
                                          gcgcTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa                                                                                                         <  1:1077786/150‑1 (MQ=255)
                                          gcgcTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGaaaaaa                                                                                                         >  2:317777/1‑150 (MQ=255)
                                             cTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACAACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAatat                                                                                                      >  2:1012595/1‑150 (MQ=255)
                                              tATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAAtata                                                                                                     <  2:933449/150‑1 (MQ=255)
                                                 gggCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAattataa                                                                                                   >  2:712917/1‑145 (MQ=255)
                                                 gggCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAcc                                                                                                  >  2:773931/1‑150 (MQ=255)
                                                 gggCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAAcc                                                                                                  >  1:733237/1‑150 (MQ=255)
                                                    ccAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCAc                                                                                               <  1:1432326/150‑1 (MQ=255)
                                                       gggAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAACTATAACCCACGAc                                                                                            >  2:444779/1‑150 (MQ=255)
                                                          aGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAc                                                                                         >  1:61874/1‑150 (MQ=255)
                                                          aGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAAc                                                                                         <  1:1709951/150‑1 (MQ=255)
                                                            ccTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTACCCACTCTGCGCCCTTCACCCCTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGCCAACCa                                                                                       >  2:1581235/1‑150 (MQ=255)
                                                            ccTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCa                                                                                       <  1:499269/150‑1 (MQ=255)
                                                            ccTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCa                                                                                       >  1:551907/1‑150 (MQ=255)
                                                            ccTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGTCGAGATCGAAAAAATATAACCCACGACAACCAt                                                                                      >  2:1510522/1‑150 (MQ=255)
                                                                 cACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAAt                                                                                  <  2:1079672/150‑1 (MQ=255)
                                                                      ggCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGCTCGAAAAAATATAACCCACGACAACCATAAATTCTgg                                                                             >  2:690146/1‑150 (MQ=255)
                                                                       gCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGc                                                                            <  2:1692740/150‑1 (MQ=255)
                                                                        ccAATATGTCTGGCGATAAATTCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGTAGGGAAACCCGCAGCACACTAATCTGGTCGAGTTCGAAAAATTATTTCCGACGACACCCTTAATTTCTGGCa                                                                           >  2:250591/1‑150 (MQ=255)
                                                                        ccAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCCCTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCCCGACAACCATAAATTCTGGCa                                                                           >  1:94611/1‑150 (MQ=255)
                                                                        ccAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTTGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCa                                                                           <  1:297350/150‑1 (MQ=255)
                                                                        ccAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATACCCCACGACAACCATAAATTcagtca                                                                           >  1:236373/1‑145 (MQ=255)
                                                                         aaaTATGTCTGTCGATAATATCGACCATGTCGGATGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTTGCGAAGGGATACCCGCAGCACACTAATCTGGTCGAGATCGATAAAATATAACCCACGACAACCATAAATTCTGGCAt                                                                          <  2:882945/149‑1 (MQ=255)
                                                                          aaTATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATg                                                                         >  2:1513262/1‑150 (MQ=255)
                                                                              tGTCTGGCTATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAt                                                                     <  1:169582/150‑1 (MQ=255)
                                                                               gTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTAACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAtt                                                                    >  2:564595/1‑150 (MQ=255)
                                                                                tCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAttt                                                                   >  2:1700278/1‑150 (MQ=255)
                                                                                 cTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACAtttt                                                                  <  2:733237/150‑1 (MQ=255)
                                                                                   ggCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAATCCCGCAGCACACTAATCTGGTCGAGATCGAAAAACTATTTCCCACGACAACCTTAAATTCTGGCTTGACTGtttgt                                                                >  1:1049849/1‑150 (MQ=255)
                                                                                   ggCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgt                                                                >  1:1023999/1‑150 (MQ=255)
                                                                                   ggCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgt                                                                >  2:771933/1‑150 (MQ=255)
                                                                                   ggCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgt                                                                >  2:738515/1‑150 (MQ=255)
                                                                                    gCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtt                                                               <  1:1275143/150‑1 (MQ=255)
                                                                                     cGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgttt                                                              <  2:737223/150‑1 (MQ=255)
                                                                                     cGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtttg                                                             <  1:1510522/150‑1 (MQ=255)
                                                                                      gATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtttg                                                             <  2:233110/150‑1 (MQ=255)
                                                                                      gATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATtttgtttg                                                             <  2:1654035/150‑1 (MQ=255)
                                                                                       aTAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGa                                                            >  1:179814/1‑150 (MQ=255)
                                                                                       aTAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGa                                                            >  1:1314720/1‑150 (MQ=255)
                                                                                        tAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaa                                                           <  1:28339/150‑1 (MQ=255)
                                                                                        tAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGaa                                                           <  1:104886/150‑1 (MQ=255)
                                                                                               gACCATGGCGGCTGTGTGAATACTCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCTAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAAt                                                    <  1:325112/150‑1 (MQ=255)
                                                                                                    tGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTg                                               <  1:502792/150‑1 (MQ=255)
                                                                                                    tGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTg                                               <  1:600859/150‑1 (MQ=255)
                                                                                                     ggcggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGa                                              >  2:1147632/1‑150 (MQ=255)
                                                                                                        ggcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTa                                           <  2:705131/150‑1 (MQ=255)
                                                                                                         gcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTaa                                          >  1:1237471/1‑150 (MQ=255)
                                                                                                         gcTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTaa                                          >  1:418616/1‑150 (MQ=255)
                                                                                                           tgtgtgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAatg                                        <  2:111012/150‑1 (MQ=255)
                                                                                                               tgAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAAtgatga                                    >  2:1545093/1‑150 (MQ=255)
                                                                                                                  aTACGCTAACCACTCTGCGCCCTTCACCACTGGCGACGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATACCCCACGACCACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGataa                                 >  1:1166523/1‑150 (MQ=255)
                                                                                                                    aCGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATc                               <  1:212233/150‑1 (MQ=255)
                                                                                                                      gCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCa                             <  2:852088/150‑1 (MQ=255)
                                                                                                                       cTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCaa                            >  2:1306897/1‑149 (MQ=255)
                                                                                                                       cTAACCACTCTGCGCCCTTCACCACTGGCGAACGGAAACCAGCAGCAAACTAATCTTGTCTAGATCTAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCAt                            <  2:1133227/150‑1 (MQ=255)
                                                                                                                         aaCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc                          <  2:962921/150‑1 (MQ=255)
                                                                                                                         aaCCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATAc                          <  1:708868/150‑1 (MQ=255)
                                                                                                                               gggtttcccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACaga                    >  2:525454/7‑150 (MQ=255)
                                                                                                                                ctGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACagag                   <  1:565271/150‑1 (MQ=255)
                                                                                                                                  gcgcCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGgc                 >  1:1617341/1‑150 (MQ=255)
                                                                                                                                    tcccTTCGCCAGTGGTGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGgcgc               >  2:1294689/2‑150 (MQ=255)
                                                                                                                                       cTTCACCACTGGCGAAGGGAAACCCGCCGCTCACTAATCTGGTCGAGATCGAAAAAATATAACCCACGCCAACCATAAATTCTGGCATGACATTTTGTTTGAATAGCAATAAGTGAGTAATGATGAAAATCCATACCCCAGAGGCGCTCa            >  1:95639/1‑150 (MQ=255)
                                                                                                                                       cTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCa            <  2:1061496/150‑1 (MQ=255)
                                                                                                                                       cTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCa            >  1:1211438/1‑150 (MQ=255)
                                                                                                                                        ttCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCAt           <  1:1669742/150‑1 (MQ=255)
                                                                                                                                        ttCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCAt           <  2:916375/150‑1 (MQ=255)
                                                                                                                                          caccacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATATGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGa         >  2:927241/1‑150 (MQ=255)
                                                                                                                                            ccacTGTCGAAGTGAAACCCGCAGCACACTAATCTGGTCGAGATCGAATAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAATAGCTATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAg       <  1:336653/150‑1 (MQ=255)
                                                                                                                                            ccacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAg       >  2:1475426/1‑150 (MQ=255)
                                                                                                                                            ccacTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAg       >  1:1241428/1‑150 (MQ=255)
                                                                                                                                              acTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGc     <  2:1083119/150‑1 (MQ=255)
                                                                                                                                              acTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAACCCATACCACAGAGGCGCTCATGAAGGc     <  1:304831/150‑1 (MQ=255)
                                                                                                                                              acTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGAGAGTAATGATGAAAATCCATACCACAGAGGCGATCATGAAGGc     >  2:1408626/1‑150 (MQ=255)
                                                                                                                                                 ggCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCTGa  >  1:1265260/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATAAAATCGACCATGGCGGCTGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACTAATCTGGTCGAGATCGAAAAAATATAACCCACGACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCTGA  >  NC_000913/1660299‑1660593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: