Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,661,943 C→A 5.0% P463H (CCC→CAC)  ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,661,9430CA 5.0% 330.3 / 2.2 110P463H (CCC→CAC) ynfFS‑ and N‑oxide reductase, A subunit, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (49/54);  new base A (3/4);  total (52/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

CTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGC  >  NC_000913/1661794‑1662091
                                                                                                                                                     |                                                                                                                                                    
cTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTTAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTccc                                                                                                                                                      >  2:1450748/1‑150 (MQ=255)
cTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTccc                                                                                                                                                      <  2:1582021/150‑1 (MQ=255)
cTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGGGATGGGGTTCGTGGAAAAGAAAAACTGGATGTccc                                                                                                                                                      >  2:1225062/1‑150 (MQ=255)
cTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGAGACACGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTccc                                                                                                                                                      >  2:1218774/1‑150 (MQ=255)
cTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCAATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGATAAAATGGATGTccc                                                                                                                                                      >  2:240253/1‑150 (MQ=255)
     aTCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCa                                                                                                                                                 <  2:720753/150‑1 (MQ=255)
      tCTGGGGGTAGAATGGTTCACGATGCTCGAGAATCCTGTAAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAAAGACCCCGACCCTTCATGGTTTTCGTGGAAAAGAAAAACAGGATGACCCCATaaa                                                                                                                                                >  1:1306953/1‑150 (MQ=255)
             gTAGAATTGTTCCCGATGCTCGAGAATCATGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTa                                                                                                                                         >  2:1240224/1‑150 (MQ=255)
              tAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTAt                                                                                                                                        <  1:430378/150‑1 (MQ=255)
              tAGAATGGTTCCCGATGCTCGAGAAGCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTAt                                                                                                                                        <  1:1242663/150‑1 (MQ=255)
               ataaTGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACAATGGTACGGAAATTACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCACATCAAGTTTTTATg                                                                                                                                       >  2:685228/3‑150 (MQ=255)
               aGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTTGATGGGGACATCCAGtttttctt                                                                                                                                       <  2:1356041/150‑4 (MQ=255)
               aGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTAt                                                                                                                                        <  1:1096138/149‑1 (MQ=255)
               aGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTAt                                                                                                                                        >  2:1096138/1‑149 (MQ=255)
                taaTGGTTCCCGATTCTCGAGAATCCTGTTAAAAGGCAGATTTCCGTATTTACATTGACAGATGCCATCGAACATGTTACTGAAATGACCGCGAACAGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCACATCAAGTTTTTATgg                                                                                                                                      <  2:115472/149‑1 (MQ=255)
                gAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATgg                                                                                                                                      >  2:1649467/1‑150 (MQ=255)
                gAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATgg                                                                                                                                      <  2:1513892/150‑1 (MQ=255)
                gAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATgg                                                                                                                                      <  2:454959/150‑1 (MQ=255)
                gAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATgg                                                                                                                                      >  2:210141/1‑150 (MQ=255)
                 aaTGGTTCCCGATGCTGGTGATTCCTGTTAAAACGCATATTTCCGTCTTTAGATGGACAGATGCCATCGACCATTTTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTTGATGGGGACATCCAGtttttctttt                                                                                                                                     <  1:1394927/150‑6 (MQ=255)
                   tGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGc                                                                                                                                   >  1:1673919/1‑150 (MQ=255)
                     gTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTa                                                                                                                                 >  2:127406/1‑150 (MQ=255)
                        cccGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCACATCAAGTTTTTATGGTGCTACGc                                                                                                                              >  1:1496976/1‑150 (MQ=255)
                          cGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATTACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCa                                                                                                                            >  2:1701620/1‑150 (MQ=255)
                               cTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACGGCGACCCGTGATGGGGTTCGGGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGACAGTTac                                                                                                                       >  2:819501/1‑150 (MQ=255)
                               cTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGCCCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAac                                                                                                                       >  2:1535200/1‑150 (MQ=255)
                               cTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAac                                                                                                                       >  2:1244040/1‑150 (MQ=255)
                                 cGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAacac                                                                                                                     <  1:210141/150‑1 (MQ=255)
                                   agaATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGCCCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACAt                                                                                                                   >  1:1155122/1‑150 (MQ=255)
                                       tCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGat                                                                                                               <  2:159161/150‑1 (MQ=255)
                                       cccTGTTAAAACGCAGATTTCCGTCTTTACATGGACTGCTGCCATCGACCAGGGTACGGAAAGGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGat                                                                                                               <  1:250524/149‑1 (MQ=255)
                                        ccTCTTAAAACGCAGAATTCCGTCTTTACATCGACAGAAGCCATCGACCATGGTACGGAAATGACCGCGACCCTTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatc                                                                                                              <  2:309503/150‑1 (MQ=255)
                                         cTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatca                                                                                                             <  1:1309549/150‑1 (MQ=255)
                                         cTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatca                                                                                                             >  2:208251/1‑150 (MQ=255)
                                            ttAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTTGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatcaatc                                                                                                          <  1:194559/150‑1 (MQ=255)
                                            ttAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatcaatc                                                                                                          <  1:1076219/150‑1 (MQ=255)
                                            ttAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatcaatc                                                                                                          <  2:824016/150‑1 (MQ=255)
                                            ttAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatcaatc                                                                                                          >  2:893425/1‑150 (MQ=255)
                                             tAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatcaatca                                                                                                         >  2:631651/1‑150 (MQ=255)
                                               aaaCGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAAc                                                                                                       >  1:595147/1‑150 (MQ=255)
                                                aaCGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACa                                                                                                      <  1:1332670/150‑1 (MQ=255)
                                                   tcAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATgg                                                                                                   >  1:252107/2‑150 (MQ=255)
                                                          tCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatc                                                                                            <  2:1227391/150‑1 (MQ=255)
                                                            cGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaa                                                                                          <  1:1244040/150‑1 (MQ=255)
                                                                tttACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATcac                                                                                      >  1:1186832/1‑150 (MQ=255)
                                                                 ttACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATcaca                                                                                     >  2:229273/1‑150 (MQ=255)
                                                                   aCATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACAcc                                                                                   >  1:919995/1‑150 (MQ=255)
                                                                      tGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCAt                                                                                >  1:483517/1‑150 (MQ=255)
                                                                         aCAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAg                                                                             >  2:1638092/1‑150 (MQ=255)
                                                                          cAGATGCCATCGACCATTGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAgg                                                                            <  1:756816/150‑1 (MQ=255)
                                                                           aGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGt                                                                           <  1:814632/150‑1 (MQ=255)
                                                                            gATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTg                                                                          >  2:788345/1‑150 (MQ=255)
                                                                                ccATCGACCATGGTACGGAAATGACCTCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGCTCAATCAACATGGCGACATCAATCACACCCATGAGGTGCtgc                                                                      >  2:106388/1‑148 (MQ=255)
                                                                                ccATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTc                                                                      <  1:979468/150‑1 (MQ=255)
                                                                                 cATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTTCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCa                                                                     >  1:826975/1‑150 (MQ=255)
                                                                                  aTCGACCATGGTTCGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCGCCATCAAGTTTTTATGGTTCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAg                                                                    <  2:1306953/150‑1 (MQ=255)
                                                                                  aTCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAg                                                                    <  1:1700450/150‑1 (MQ=255)
                                                                                    cGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGa                                                                  <  1:1216771/150‑1 (MQ=255)
                                                                                     gACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGAt                                                                 <  1:1039576/150‑1 (MQ=255)
                                                                                      aCCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATg                                                                >  2:1128776/1‑150 (MQ=255)
                                                                                       ccATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTACCACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGa                                                               >  1:1588447/1‑150 (MQ=255)
                                                                                        cATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGAc                                                              <  2:1315104/150‑1 (MQ=255)
                                                                                          tGGTACGGAAATGAACGAGACCGGTGATGGTGTTCGTGGAAAAGAAAAACTGGAGGTCACCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAg                                                            <  1:685228/150‑1 (MQ=255)
                                                                                              aCGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAACCTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCCCACCCATGAGGTGCTTCAGGATGACAGCAAg                                                        >  1:311865/1‑150 (MQ=255)
                                                                                              aCGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAg                                                        <  2:465175/150‑1 (MQ=255)
                                                                                              aCGGAAATGACCGCGACCCGTGATGCTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAg                                                        <  2:424819/150‑1 (MQ=255)
                                                                                               cGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGt                                                       <  1:1395805/150‑1 (MQ=255)
                                                                                                ggAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTg                                                      <  2:1504792/150‑1 (MQ=255)
                                                                                                ggAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTg                                                      <  1:378131/150‑1 (MQ=255)
                                                                                                    aTGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCgag                                                  <  2:1409284/150‑1 (MQ=255)
                                                                                                      gACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGAAATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAgat                                                >  2:597679/1‑150 (MQ=255)
                                                                                                       aCCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCTATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAgatg                                               >  1:120002/1‑150 (MQ=255)
                                                                                                       aCCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAgatg                                               <  2:1673919/150‑1 (MQ=255)
                                                                                                         cgcgACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAgatgat                                             <  2:1115056/150‑1 (MQ=255)
                                                                                                           cgACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGAttg                                           <  1:1649467/150‑1 (MQ=255)
                                                                                                                     tGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGa                                 >  2:1007435/1‑150 (MQ=255)
                                                                                                                     tGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTGGGCATTGa                                 >  2:69805/1‑150 (MQ=255)
                                                                                                                       gtgtTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGAcc                               >  1:567218/1‑150 (MQ=255)
                                                                                                                       gtgtTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGAcc                               <  1:1600009/150‑1 (MQ=255)
                                                                                                                       gtgtTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGAcc                               <  1:893425/150‑1 (MQ=255)
                                                                                                                         gtTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCAc                             >  1:1634589/1‑150 (MQ=255)
                                                                                                                           tCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACtt                           >  2:1006946/1‑150 (MQ=255)
                                                                                                                             gTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCa                         <  1:955749/150‑1 (MQ=255)
                                                                                                                               ggAAAAGAAAAACTGTCTGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACCCCCATGAGGTGCTTCAGGAAGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATg                       <  2:1038755/150‑1 (MQ=255)
                                                                                                                               ggAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATg                       <  2:595147/150‑1 (MQ=255)
                                                                                                                               ggAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATg                       >  1:623827/1‑150 (MQ=255)
                                                                                                                                gaaaagaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGa                      >  2:683928/1‑150 (MQ=255)
                                                                                                                                 accataaaaaCTTGATGGGGACATCCAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGAc                     >  2:1523629/6‑150 (MQ=255)
                                                                                                                                 aaaagaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATgcc                     >  1:1490039/1‑148 (MQ=255)
                                                                                                                                 aaaagaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGAc                     >  2:1169445/1‑150 (MQ=255)
                                                                                                                                   aagaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGTTGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACgg                   >  1:114202/1‑150 (MQ=255)
                                                                                                                                   aagaaaaACTGGATGTCCCAATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACgg                   <  1:1240224/150‑1 (MQ=255)
                                                                                                                                    agaaaaACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGc                  >  1:518728/1‑150 (MQ=255)
                                                                                                                                             ggATGTCCCCATCAAGTTTTTATGGTGATACGCCAGTAACACATTGATAAATAAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGAGAGATGATTGTTGGCATTGACAACTTCATGACGGCCTCGGCTaa         >  1:1089596/1‑150 (MQ=255)
                                                                                                                                              gATGTCCCCCTCAAGGTTTTATGGTCCTACGCCAGTCACACATTGATCACTCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGTGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAg        <  2:349771/150‑1 (MQ=255)
                                                                                                                                               aTGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGt       <  2:1335496/150‑1 (MQ=255)
                                                                                                                                               aTGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGt       <  2:1186832/150‑1 (MQ=255)
                                                                                                                                                   ccccATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACAGCCATGAGGTGCTTCTGGATGTCAGCACGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTg   <  2:1367897/150‑1 (MQ=255)
                                                                                                                                                    cccATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGc  <  1:1638092/150‑1 (MQ=255)
                                                                                                                                                    cccATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGc  <  1:548963/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGC  >  NC_000913/1661794‑1662091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑