Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,674,177 G→T 6.9% E89* (GAG→TAG) ‡ tqsA → pheromone AI‑2 transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,1770GT 6.9% 287.9 / 10.0 101E89* (GAG→TAG) ‡tqsApheromone AI‑2 transporter
Reads supporting (aligned to +/- strand):  ref base G (44/50);  new base T (4/3);  total (48/53)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.19e-01

Only 100 of 101 total aligned reads displayed.

GCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCAC  >  NC_000913/1674030‑1674325
                                                                                                                                                   |                                                                                                                                                     
gCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAg                                                                                                                                                     <  2:1304854/150‑1 (MQ=255)
gCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAg                                                                                                                                                     >  2:365822/1‑150 (MQ=255)
  attattTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGtt                                                                                                                                                   <  1:1633926/150‑1 (MQ=255)
   ttatttatTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTg                                                                                                                                                  <  2:948686/150‑1 (MQ=255)
   ttatttatTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGATGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATTGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTg                                                                                                                                                  <  2:198204/150‑1 (MQ=255)
    tatttattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGa                                                                                                                                                 <  1:1418519/150‑1 (MQ=255)
     atttattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGAc                                                                                                                                               >  2:954978/1‑150 (MQ=255)
     atttattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGAc                                                                                                                                               >  1:463947/1‑150 (MQ=255)
     atttattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGAc                                                                                                                                               >  1:1701954/1‑150 (MQ=255)
        tattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTTCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGAcgcg                                                                                                                                            <  2:673033/150‑1 (MQ=255)
        tattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGTCAACTCGTTGagcgcg                                                                                                                                            <  2:1672916/150‑6 (MQ=255)
        tattGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGTCAACTCGTTGagcgcg                                                                                                                                            <  1:57835/150‑6 (MQ=255)
         attGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGGGTTGCTATTAGCTTATCTGGGTTCCGCGTCAACTCGTTGAGCGCgg                                                                                                                                           <  1:47143/150‑1 (MQ=255)
             cTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGt                                                                                                                                       >  1:652882/1‑150 (MQ=255)
              tGTTCTTCTTAACCCGCTGGGGCAACACCTGGTCCGCGGGCGTGTGCCGCGTGTACTGGCGGTGGCGATTTTGATGACCATCATCGTGAGGGCGATGGTGTTGCTATTAGCTGATCTGGGTTCCGCGCTCAACGCGTTGACGCGGACGtt                                                                                                                                      <  2:835146/150‑1 (MQ=255)
                 tATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTAcc                                                                                                                                   >  2:415533/1‑150 (MQ=255)
                  aTTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCg                                                                                                                                  >  2:214301/1‑150 (MQ=255)
                    tCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACCGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCa                                                                                                                                >  1:1708207/1‑150 (MQ=255)
                      ttAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAat                                                                                                                              >  1:1365124/1‑150 (MQ=255)
                       tAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAata                                                                                                                             >  2:1073495/1‑150 (MQ=255)
                       tAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAata                                                                                                                             >  1:606574/1‑150 (MQ=255)
                        aaCCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAatat                                                                                                                            >  1:275881/1‑150 (MQ=255)
                           ccGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGc                                                                                                                         <  1:1691473/150‑1 (MQ=255)
                              cTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAAc                                                                                                                      >  2:271602/1‑150 (MQ=255)
                                ggTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCATTATCGCAActc                                                                                                                    >  1:284411/1‑150 (MQ=255)
                                ggTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAActc                                                                                                                    >  2:683764/1‑150 (MQ=255)
                                ggTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAActc                                                                                                                    <  2:1465379/150‑1 (MQ=255)
                                   gCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGCCCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCtat                                                                                                                 >  2:1248036/1‑150 (MQ=255)
                                    cAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCtatt                                                                                                                >  2:746520/1‑150 (MQ=255)
                                     aaCCCATGGTCCGCTGGCGGGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGTGTTGACGCGGACGTTACCGCAATATCGCAACTCtatta                                                                                                               <  2:240611/150‑1 (MQ=255)
                                     aaCACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGCCGTTAACGCAATATCGCAACTCtatta                                                                                                               >  2:931215/1‑150 (MQ=255)
                                     aaCACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCtatta                                                                                                               <  2:463947/150‑1 (MQ=255)
                                     aaCACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCtatta                                                                                                               <  2:1701954/150‑1 (MQ=255)
                                         caTGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGATGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGAc                                                                                                           <  2:1244940/150‑1 (MQ=255)
                                         aaTGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGAc                                                                                                           <  2:877883/149‑1 (MQ=255)
                                          aTGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGAcg                                                                                                          <  1:1131677/150‑1 (MQ=255)
                                          aTGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGATTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGAAGTTACCGCAATATCGCAACTAGATTATGAcg                                                                                                          >  2:210109/1‑150 (MQ=255)
                                          aTGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGGCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGAcg                                                                                                          <  1:89855/150‑1 (MQ=255)
                                                    ggCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAg                                                                                                >  1:1424649/1‑150 (MQ=255)
                                                       gtgtGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAgcgc                                                                                             <  2:1338883/150‑1 (MQ=255)
                                                       gtgtGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAgcgc                                                                                             <  1:661894/150‑1 (MQ=255)
                                                        tgtgCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCt                                                                                            <  1:124656/150‑1 (MQ=255)
                                                         gtgCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCCTCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCCACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCtt                                                                                           <  1:543639/150‑1 (MQ=255)
                                                          tgCCGCGTGTACTGGCGGTGTCGATTTTGATGACGATCATCTTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCTCTCATCGAGTTGACGCGGACGTTACCGCAATATCGCTACTCTATTATGACGCCGCTGCAAGCGCTTg                                                                                          <  1:238532/150‑1 (MQ=255)
                                                           gCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGAGATGCTGTTCCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTCACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAACCGCTTGa                                                                                         >  2:1330389/1‑150 (MQ=255)
                                                             cgcgTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAAc                                                                                       >  1:223383/1‑150 (MQ=255)
                                                             cgcgTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAAc                                                                                       <  1:1731327/150‑1 (MQ=255)
                                                             cgcgTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCAATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCTAGCGCTTGAAc                                                                                       <  1:271602/150‑1 (MQ=255)
                                                               cgTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGATCCg                                                                                     >  2:1313645/1‑150 (MQ=255)
                                                                  gtACTGGCGGTGTCGATTTTGATGACCTTCATCGTGATGGCGATGGTGTTGATATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCTATATCGCAACTGTATTATGACGCCGCTGCAAGCGCTTGAACCgttg                                                                                  <  1:554862/150‑1 (MQ=255)
                                                                  gtACTGGCGGTGGCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCgttg                                                                                  <  2:908073/150‑1 (MQ=255)
                                                                     cTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGttgttg                                                                               >  1:1606397/1‑150 (MQ=255)
                                                                      tGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGc                                                                              <  2:500740/150‑1 (MQ=255)
                                                                      tGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGc                                                                              <  1:404909/150‑1 (MQ=255)
                                                                       ggCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCa                                                                             <  1:881921/150‑1 (MQ=255)
                                                                             gtCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGt                                                                       <  2:545753/150‑1 (MQ=255)
                                                                             gtCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGt                                                                       <  2:1608348/150‑1 (MQ=255)
                                                                             gtCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGt                                                                       >  1:424355/1‑150 (MQ=255)
                                                                               cGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAAcg                                                                          <  2:62809/145‑1 (MQ=255)
                                                                               cGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAAcg                                                                          >  1:62809/1‑145 (MQ=255)
                                                                               cGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAg                                                                     >  1:8508/1‑150 (MQ=255)
                                                                                  ttttGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGa                                                                  >  1:249717/1‑150 (MQ=255)
                                                                                         gaCAATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGATACCGCAAGATCGCAACTCTTTTATGACGCCGCTGCAAGAGCGTGAAACGTTGGTGCAACGCGTAGGGATTGACGt                                                           >  2:131141/1‑150 (MQ=255)
                                                                                           ccATCATCGTGATGGCGATGGTGTTGCTAATAGCTTATCTGGGTTCCGCGCTCAACGAGTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGtctc                                                        <  1:129247/150‑1 (MQ=255)
                                                                                                atcGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTAAAGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTAAGtt                                                    >  1:238761/1‑150 (MQ=255)
                                                                                                  cGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGa                                                  >  1:1004145/1‑150 (MQ=255)
                                                                                                   gTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATCTCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGAc                                                 <  1:1298239/150‑1 (MQ=255)
                                                                                                   gTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGAc                                                 <  2:665031/150‑1 (MQ=255)
                                                                                                   gTGAGGGCGATTGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGAGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGAc                                                 <  1:1159759/150‑1 (MQ=255)
                                                                                                    tGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGAcc                                                <  2:1326501/150‑1 (MQ=255)
                                                                                                     gATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCa                                               >  2:448653/1‑150 (MQ=255)
                                                                                                      aTGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAg                                              <  1:954978/150‑1 (MQ=255)
                                                                                                      aTGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAg                                              <  2:275881/150‑1 (MQ=255)
                                                                                                       tGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGc                                             >  2:721359/1‑150 (MQ=255)
                                                                                                       tGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGc                                             >  1:1190514/1‑150 (MQ=255)
                                                                                                            aTGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGgcg                                        <  2:1287014/150‑1 (MQ=255)
                                                                                                                tgtTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGAAGTGGGAGTTGACCAGCTGGCGCAtt                                    >  1:954640/1‑150 (MQ=255)
                                                                                                                    gCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTatat                                >  2:1314707/1‑150 (MQ=255)
                                                                                                                     cTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATAtt                               <  2:1265170/150‑1 (MQ=255)
                                                                                                                     cTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCGTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATAtt                               <  2:1711201/150‑1 (MQ=255)
                                                                                                                      tATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGCCGTCTCAGTTGACCAGCTGGCGCAGTATATTg                              >  2:125908/1‑150 (MQ=255)
                                                                                                                       aTTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCCACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGa                             >  2:1608446/1‑150 (MQ=255)
                                                                                                                         tAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATAACGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATc                           <  2:826480/150‑1 (MQ=255)
                                                                                                                          aGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATcc                          <  2:1250391/150‑1 (MQ=255)
                                                                                                                          aGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATcc                          <  1:948358/150‑1 (MQ=255)
                                                                                                                          aGCTTATCTGGGATCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATcc                          <  2:312376/150‑1 (MQ=255)
                                                                                                                           gCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCg                         <  2:50269/150‑1 (MQ=255)
                                                                                                                                 gtaacgtCCGCGTCAACTCGTTGAGCGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAAcgcg                   >  2:1140636/7‑150 (MQ=255)
                                                                                                                                    ggTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGGAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACgcggcg                >  1:1068914/1‑150 (MQ=255)
                                                                                                                                    ggTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACgcggcg                <  1:365822/150‑1 (MQ=255)
                                                                                                                                     gTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCGAACGCGGCGAt              >  1:1662725/1‑150 (MQ=255)
                                                                                                                                     gTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCGCAGTTGACCAGCTGGCGCATTATATTGATCGAACGCGGCGAt              >  1:1669170/1‑150 (MQ=255)
                                                                                                                                     cgtCCGCGTCAACTCGTTGAGCGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGa               >  2:1692312/3‑150 (MQ=255)
                                                                                                                                     cgtCCGCGTCAACTCGTTGAGCGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGa               >  1:513672/3‑150 (MQ=255)
                                                                                                                                        ccGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGa            >  2:1253677/1‑150 (MQ=255)
                                                                                                                                         cgcgCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGGTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGAc           <  2:284411/150‑1 (MQ=255)
                                                                                                                                         ccgcgtCAACTCGTTGAGCGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGa            >  2:1704275/6‑150 (MQ=255)
                                                                                                                                               cAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCt     >  2:803115/1‑150 (MQ=255)
                                                                                                                                                 aCGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCa   <  1:683764/150‑1 (MQ=255)
                                                                                                                                                  cGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCCGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCAc  >  1:1043463/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGCTGCAAGCGCTTGAACCGTTGTTGCAACGCGTAGGGATTGACGTCTCAGTTGACCAGCTGGCGCATTATATTGATCCGAACGCGGCGATGACGTTGCTCAC  >  NC_000913/1674030‑1674325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: