Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,674,955 A→T 6.6% intergenic (+8/+17) tqsA → / ← pntB pheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,9550AT 6.6% 186.0 / 2.8 70intergenic (+8/+17)tqsA/pntBpheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (36/29);  new base T (2/3);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

GTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAATCACG  >  NC_000913/1674806‑1675104
                                                                                                                                                     |                                                                                                                                                     
gTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGTGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTca                                                                                                                                                       >  2:1616807/1‑150 (MQ=255)
gTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGTGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTca                                                                                                                                                       >  2:1602987/1‑150 (MQ=255)
 ttGATTTTTTGGGGATGGTTGTTAGGACCGGTGGTTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcag                                                                                                                                                      <  2:1241896/150‑1 (MQ=255)
 ttGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcag                                                                                                                                                      <  2:1389924/150‑1 (MQ=255)
  agATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagc                                                                                                                                                     >  1:1397218/2‑150 (MQ=255)
   gATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACATTTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATAGCAGTACTGTTAAGCGATCTCAATAAAGAGTTACGGCCTcagaa                                                                                                                                                    >  1:1029627/1‑148 (MQ=255)
   gATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagca                                                                                                                                                    >  1:1569507/1‑150 (MQ=255)
    aTTTTTTGGGGATGGTTGTTAGGCCCGGTGGGTATGCTGCTTTCAGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCATAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagcag                                                                                                                                                   >  1:1535922/1‑150 (MQ=255)
    aTTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTGCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagcag                                                                                                                                                   <  1:505611/150‑1 (MQ=255)
      tttttGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCagag                                                                                                                                                 >  1:391904/1‑150 (MQ=255)
      tttttGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTGTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCagag                                                                                                                                                 <  2:720302/150‑1 (MQ=255)
        tttGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGGCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGc                                                                                                                                               <  1:917647/150‑1 (MQ=255)
              gATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                                                                         <  1:1659845/150‑2 (MQ=255)
              gATGGGTGGTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCCTGAACAAACAGCGGGAGGGCAAAGCATCGCCGATCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                                                                         <  1:1038766/150‑2 (MQ=255)
                tGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                                                                       >  1:811211/1‑150 (MQ=255)
                tGGTTGTTAGGACCGGGGGGTATGCTGCGTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                                                                       <  1:869281/150‑1 (MQ=255)
                  gttgttAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGtt                                                                                                                                     >  2:1616644/1‑150 (MQ=255)
                  gttgttAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGtt                                                                                                                                     >  2:959005/1‑150 (MQ=255)
                  gttgttAGGACCGGTGGGTATGCTGCTTTCCGAGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGtt                                                                                                                                     <  2:184996/150‑1 (MQ=255)
                       tAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCATTAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACaga                                                                                                                                >  1:1397887/1‑150 (MQ=255)
                       tAGGACCGGTGGCTAAGCTGCTTTCCTTGCCGTTGACAATTATTGTAAAAATTGCGCTTGAACAAACAGCGGGAGGTCACAGCATCGACGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGACTGGGCCGTCAGGGTTACaca                                                                                                                                >  2:483737/1‑148 (MQ=255)
                        aGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACagag                                                                                                                               >  1:1680048/1‑150 (MQ=255)
                           aCCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCtt                                                                                                                            <  1:13232/150‑1 (MQ=255)
                                  ggTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGAt                                                                                                                     >  2:377255/1‑150 (MQ=255)
                                    tATGCTGCTTTCCGTGTCGTTTACAATTTTTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGGATCGCCGTTCTGTTAAGGTATCTCAATATAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTg                                                                                                                   <  2:1701977/150‑1 (MQ=255)
                                    tATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGATGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTg                                                                                                                   <  2:1729286/150‑1 (MQ=255)
                                    tATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTg                                                                                                                   <  1:1568295/150‑1 (MQ=255)
                                    tATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTg                                                                                                                   <  2:1056923/150‑1 (MQ=255)
                                      tgctgcTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGGGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCa                                                                                                                 <  2:627709/150‑1 (MQ=255)
                                        ctgctTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCCGGATTGCATc                                                                                                               >  2:1235521/1‑150 (MQ=255)
                                        ctgctTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCATGATTGCATc                                                                                                               >  2:1469555/1‑150 (MQ=255)
                                        ctgctTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATc                                                                                                               >  2:1137611/1‑150 (MQ=255)
                                         tgctTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGTGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATcc                                                                                                              <  1:1616807/150‑1 (MQ=255)
                                         tgctTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGTGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATcc                                                                                                              <  1:1602987/150‑1 (MQ=255)
                                                cGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTgg                                                                                                       >  2:746741/1‑150 (MQ=255)
                                                      gtcgACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTgg                                                                                                 >  1:547672/4‑150 (MQ=255)
                                                      gtagACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTgg                                                                                                 >  1:547668/4‑150 (MQ=255)
                                                      gTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGCGCTTTCAGGATTGCATCCACGCTGGCTTTgg                                                                                                 >  2:1146516/1‑150 (MQ=255)
                                                       ttGACAATTATTGTCAAAATTGCTCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTTTTAAGCGATCTCAATAATGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGc                                                                                                <  1:1400638/150‑1 (MQ=255)
                                                           cAATTATTGTCAAAATTGCGCTTGAACAAACAGCGCGAGGTCAAAGCATGGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCACAGGCCGGCAGCGATACAGCGCGTACAGGATTGCCTACCCCCTGGCTGTGGCGTCa                                                                                            >  1:299526/1‑150 (MQ=255)
                                                                 ttGTCTAAATTGCGCTTGAACTAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAAc                                                                                      <  1:420199/150‑1 (MQ=255)
                                                                   gTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCATTAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAg                                                                                    >  2:829828/1‑150 (MQ=255)
                                                                   gTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAg                                                                                    >  2:1555546/1‑150 (MQ=255)
                                                                        aaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAtgt                                                                               <  2:1371113/150‑1 (MQ=255)
                                                                         aTTGCGCTTGAAAAAACAGCGGGATGTCTAAGAATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTCCAGATGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAtgtg                                                                              <  1:864905/150‑1 (MQ=255)
                                                                           tGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTggg                                                                            >  1:1492949/1‑150 (MQ=255)
                                                                                 tGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGtttt                                                                      >  2:689144/1‑150 (MQ=255)
                                                                                  gAACAAACCGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCCGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGGGGGTGTTTTc                                                                     >  2:134178/1‑150 (MQ=255)
                                                                                   aacaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGATAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGCTGTTTTcc                                                                    >  1:599543/1‑150 (MQ=255)
                                                                                    acaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCt                                                                   <  1:1616644/150‑1 (MQ=255)
                                                                                    acaaacaGCGGGAGGTCAAAGCAGCGCCGAGCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCt                                                                   <  1:173005/150‑1 (MQ=255)
                                                                                         caGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaag                                                              <  1:407539/150‑1 (MQ=255)
                                                                                                 ggTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTAGAAGAACAGCgg                                                      >  1:703064/1‑150 (MQ=255)
                                                                                                  gTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCggg                                                     >  2:1474176/1‑150 (MQ=255)
                                                                                                    cAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGtt                                                   >  1:1146009/1‑150 (MQ=255)
                                                                                                    cAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGAATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTACTTGAAGAACAGCGGGtt                                                   >  2:529478/1‑150 (MQ=255)
                                                                                                       cgCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTATCCGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTg                                                <  2:351588/149‑1 (MQ=255)
                                                                                                        gCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCGGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGc                                               >  2:278458/1‑150 (MQ=255)
                                                                                                         cATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGGCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGACGAACAGCGGGTTTTGca                                              <  1:1055579/150‑1 (MQ=255)
                                                                                                          aTCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGcac                                             <  1:171673/150‑1 (MQ=255)
                                                                                                              ccGTTCTGTTAAGAGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCa                                         <  1:36807/150‑1 (MQ=255)
                                                                                                               cGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAg                                        >  1:1400511/1‑150 (MQ=255)
                                                                                                                  tCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCAt                                     >  2:1444600/1‑150 (MQ=255)
                                                                                                                  tCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCAt                                     >  1:585354/1‑150 (MQ=255)
                                                                                                                    tGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAg                                   <  1:959005/150‑1 (MQ=255)
                                                                                                                       tAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACCGCGGGTTTTGCACACCAGCATCGCCa                                >  1:133250/1‑150 (MQ=255)
                                                                                                                       tAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGACAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCTAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCa                                >  1:127999/1‑150 (MQ=255)
                                                                                                                        aaGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAg                               <  2:587799/150‑1 (MQ=255)
                                                                                                                           cGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAgtgt                            >  2:556284/1‑150 (MQ=255)
                                                                                                                              tctcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCa                         <  2:586240/150‑1 (MQ=255)
                                                                                                                                tcATTAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATc                       <  1:829828/150‑1 (MQ=255)
                                                                                                                                 cAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGATCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAAAAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCg                      >  2:222765/1‑150 (MQ=255)
                                                                                                                                        ccctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGttt               >  2:223161/4‑150 (MQ=255)
                                                                                                                                         agTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTa              <  1:762225/150‑1 (MQ=255)
                                                                                                                                            gACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAg           >  1:1410266/1‑150 (MQ=255)
                                                                                                                                            gACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAg           <  2:391904/150‑1 (MQ=255)
                                                                                                                                            gACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAg           >  1:970388/1‑150 (MQ=255)
                                                                                                                                              cGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGGTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGATCAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGAc         <  2:1397218/150‑1 (MQ=255)
                                                                                                                                              cGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCTGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGAc         >  1:903626/1‑150 (MQ=255)
                                                                                                                                                     agcagAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAATCACg  <  2:1400511/150‑1 (MQ=255)
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GTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGTTTTGCACACCAGCATAGCCAGTGTTCATCGAACGTTTAAAGACAATCACG  >  NC_000913/1674806‑1675104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: