Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,693,417 G→C 5.2% L283V (CTG→GTG) ‡ uidB ← glucuronide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,693,4170GC 5.2% 276.9 / 4.4 91L283V (CTG→GTG) ‡uidBglucuronide transporter
Reads supporting (aligned to +/- strand):  ref base G (36/49);  new base C (2/3);  total (39/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

ACCAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGATGCACA  >  NC_000913/1693268‑1693566
                                                                                                                                                     |                                                                                                                                                     
aCCAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAg                                                                                                                                                       <  1:914938/150‑1 (MQ=255)
aCCAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAg                                                                                                                                                       <  1:920965/150‑1 (MQ=255)
     ttGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGCCGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATACCCGGCACCAGCGGTg                                                                                                                                                  <  1:1078997/150‑1 (MQ=255)
     ttGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTg                                                                                                                                                  <  2:992283/150‑1 (MQ=255)
     ttGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTg                                                                                                                                                  <  2:1225693/150‑1 (MQ=255)
       gAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGCTCCTCGCGACCATCCCCGGCACCAGCGGTGcc                                                                                                                                                >  1:406337/1‑150 (MQ=255)
         aGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGa                                                                                                                                              >  2:873569/1‑150 (MQ=255)
          gCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCACTCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCGCTGGTGCCggg                                                                                                                                             <  2:935961/150‑3 (MQ=255)
          gCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCGCTGGTGCCggg                                                                                                                                             <  1:1011251/150‑3 (MQ=255)
            gATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCGCTGGTGCCgggga                                                                                                                                           <  2:1259987/150‑5 (MQ=255)
            gATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTAACCGATCCACGCGACCATCCCCGGCACCAGCGGTGCCGATGc                                                                                                                                           >  1:705301/1‑150 (MQ=255)
             aTGGCCAACGCAACCACCGCCACCGGCAGTGACCAGACGGAAACCCAGCAGCACAGCAGATCACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACGATCCCCGGCACCAGCGGTGCCGATGcc                                                                                                                                          <  1:53359/150‑1 (MQ=255)
             aTGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGcc                                                                                                                                          <  1:510694/150‑1 (MQ=255)
                gCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCCCCCGCGGTGCCGATGCcacc                                                                                                                                       >  1:471695/1‑149 (MQ=255)
                 ccAACGCAACCACCGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCACAGCGCCAATCAGGCAGGTATTCTTTTTACCGCTCCTCGCGACCATCCCCGGCCCCAGCGGTGCCGATGCcaccg                                                                                                                                      >  1:305082/1‑148 (MQ=255)
                 ccAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAg                                                                                                                                      >  1:919695/1‑150 (MQ=255)
                    aCGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACATCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTac                                                                                                                                   >  1:634973/1‑150 (MQ=255)
                    aCGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAACCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTac                                                                                                                                   <  2:918705/150‑1 (MQ=255)
                     cGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTacc                                                                                                                                  <  1:698342/150‑1 (MQ=255)
                      gCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTacca                                                                                                                                 <  2:13857/150‑1 (MQ=255)
                       caaccaacGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTaccaa                                                                                                                                <  1:155546/150‑1 (MQ=255)
                       caaccaacGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTaccaa                                                                                                                                >  2:1109190/1‑150 (MQ=255)
                       caaccaacGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTATTTACCGATCCTCGCGACCATCCCCGGCACCCTCGGTGCCGATGCCACAGTacaaa                                                                                                                                >  2:1483611/1‑150 (MQ=255)
                           caacGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGAAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCAACAGTACCAACCAg                                                                                                                            >  2:1100474/1‑150 (MQ=255)
                            aacGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTACCCAGCAACGCGCCAATCAGGAAGGAATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAgg                                                                                                                           <  1:1050412/150‑1 (MQ=255)
                              cGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCATATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGtt                                                                                                                         <  2:388307/150‑1 (MQ=255)
                               gCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGttt                                                                                                                        >  1:295157/1‑150 (MQ=255)
                                ccACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGtttt                                                                                                                       <  1:246857/150‑1 (MQ=255)
                                  aCCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGc                                                                                                                     <  1:416442/150‑1 (MQ=255)
                                   ccGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCCCCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCa                                                                                                                    >  2:1576239/1‑150 (MQ=255)
                                     ggCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCAcc                                                                                                                  <  1:7295/150‑1 (MQ=255)
                                     ggCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCAcc                                                                                                                  <  2:1017931/150‑1 (MQ=255)
                                     ggCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCAcc                                                                                                                  <  2:1114508/150‑1 (MQ=255)
                                               aGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGca                                                                                                        <  1:1040899/150‑1 (MQ=255)
                                                gACGGAAACCAAGAAGAACAGAAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCCGCGGTGCCGATGCCACAGTACCACCCAGGTTTTGCACCAGTACCAGcac                                                                                                       >  2:1189989/1‑150 (MQ=255)
                                                  cGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAg                                                                                                     <  1:873569/150‑1 (MQ=255)
                                                  cGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGAAACGCGCCAATCAGGACGGTTTTCTTTTTACCGAACCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAg                                                                                                     <  2:1302346/150‑1 (MQ=255)
                                                    gAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTg                                                                                                   <  2:37838/150‑1 (MQ=255)
                                                       aCCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAAc                                                                                                <  1:1719600/150‑1 (MQ=255)
                                                       aCCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAAc                                                                                                <  1:1147576/150‑1 (MQ=255)
                                                       aCCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAAc                                                                                                >  1:983369/1‑150 (MQ=255)
                                                         ccAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAg                                                                                              <  2:983369/150‑1 (MQ=255)
                                                          cAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGc                                                                                             >  2:864703/1‑150 (MQ=255)
                                                           agaagaACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCAGAGTACCCACCAGGGTTTGGCCCAGTACCAGGACAGTGAACAGcc                                                                                            >  1:1144986/1‑150 (MQ=255)
                                                            gaagaaAAGCAGATAACCGCAGGGTCCCAGCAACGCGCCAATCAGGAAGGTTTTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGccc                                                                                           <  2:277367/150‑1 (MQ=255)
                                                                    gcagATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAt                                                                                   <  1:864703/150‑1 (MQ=255)
                                                                     cagATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGGTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCAtt                                                                                  <  2:18643/150‑1 (MQ=255)
                                                                       gATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGCACAGCCCGGTATCATTTa                                                                                >  1:1693103/1‑150 (MQ=255)
                                                                       gATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTa                                                                                >  1:952881/1‑150 (MQ=255)
                                                                       gATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTa                                                                                <  2:471439/150‑1 (MQ=255)
                                                                         tAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCCGCACAGTGAACAGCCCGGTATCATTTAac                                                                              >  2:818910/1‑150 (MQ=255)
                                                                         tAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAac                                                                              <  1:1374807/150‑1 (MQ=255)
                                                                           aCCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAacac                                                                            >  1:1018620/1‑150 (MQ=255)
                                                                            ccGCAGGTTCCCCGCAAAGCGCCAATCAGGAAGGGATTCTTTTTCCCGCTCCTCGCGCCCCTCCCCGGCACCCGCGGTGCCGATGCCACAGTACCAACCCGGTTTTGCACCAGTACCAGCACAGGGCACAGCCCGGTATTATTTAAcaca                                                                           >  2:209955/1‑150 (MQ=255)
                                                                            ccGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCCACCAGGTTTTGCACCAGTCCCAGCACAGGGAACAGCCCGGGATCATTTAAcaca                                                                           >  2:400562/1‑150 (MQ=255)
                                                                                 ggTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCTCCAGTACCAGCACAGTGATCAGCCCGGTATCATTTAAGACATcgcg                                                                      >  2:887855/1‑150 (MQ=255)
                                                                                     cccAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACg                                                                  <  2:504549/150‑1 (MQ=255)
                                                                                     cccAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACg                                                                  >  2:525376/1‑150 (MQ=255)
                                                                                      ccAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGCTGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACCGCCCCGTATCATTTAACACATAGCGCACGt                                                                 >  2:1042541/1‑150 (MQ=255)
                                                                                      ccAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGt                                                                 >  1:769678/1‑150 (MQ=255)
                                                                                      ccAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGt                                                                 >  2:315552/1‑150 (MQ=255)
                                                                                               cgcCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAacg                                                        >  2:1116563/1‑150 (MQ=255)
                                                                                                 ccAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAacgac                                                      <  1:477967/150‑1 (MQ=255)
                                                                                                  cAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAacgacg                                                     >  2:1167359/1‑150 (MQ=255)
                                                                                                  cAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAacgacg                                                     >  1:1207687/1‑150 (MQ=255)
                                                                                                      cAGGAAGGTTTTCTTTTTACCGATCCTTGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGgc                                                 <  2:540642/150‑1 (MQ=255)
                                                                                                      cAGGAAGGTATTCTATTTACCGAGCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGgc                                                 <  2:547522/150‑1 (MQ=255)
                                                                                                      cAGGAAGGAATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTCTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGgc                                                 <  2:683789/150‑1 (MQ=255)
                                                                                                          aaGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTg                                             >  2:398892/1‑150 (MQ=255)
                                                                                                            ggTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGAc                                           <  2:1464537/150‑1 (MQ=255)
                                                                                                             gTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGAcc                                          >  2:385776/1‑150 (MQ=255)
                                                                                                              tATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACCGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCg                                         >  2:724117/1‑150 (MQ=255)
                                                                                                               aTTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGc                                        <  1:903752/150‑1 (MQ=255)
                                                                                                                     tttACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGt                                  <  1:1100474/150‑1 (MQ=255)
                                                                                                                     ttaaCCGATCCTCGCGACCATCCCCGGCACCAGCGGGGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGt                                  <  1:1015353/147‑1 (MQ=255)
                                                                                                                       tACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCg                                <  2:295157/150‑1 (MQ=255)
                                                                                                                          cGATCCTCTCGCCCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGaaa                             <  2:1596950/150‑1 (MQ=255)
                                                                                                                          cGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGaaa                             <  1:1576239/150‑1 (MQ=255)
                                                                                                                          cGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGaaa                             <  1:973419/150‑1 (MQ=255)
                                                                                                                           gATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAAt                            >  1:447408/1‑150 (MQ=255)
                                                                                                                            aTCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATc                           <  2:739963/150‑1 (MQ=255)
                                                                                                                             accTCGCGACCATCACCGGCACCAGCGGGGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGAACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCa                          <  1:1042541/149‑1 (MQ=255)
                                                                                                                                 cgcgACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGcac                      >  2:106552/1‑150 (MQ=255)
                                                                                                                                 cgcgACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGGTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGcac                      <  2:1183982/150‑1 (MQ=255)
                                                                                                                                   cgACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGcacac                    <  2:230060/150‑1 (MQ=255)
                                                                                                                                    gACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGcacaca                   <  2:919695/150‑1 (MQ=255)
                                                                                                                                      ccATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGcacacacc                 >  2:1336439/1‑149 (MQ=255)
                                                                                                                                        ggcatcGGCACCGCTGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCCCAGTGAACAGCCCGGTATCAATTAACACATAGCGCCCGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGCAATCAGCACACCCAgc               >  1:1413502/6‑150 (MQ=255)
                                                                                                                                        aTCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGCCGCGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAgc               >  1:451674/1‑150 (MQ=255)
                                                                                                                                          catcGGCACCGCTGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGCCGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACCgcgc             >  2:393075/4‑150 (MQ=255)
                                                                                                                                            ccGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCAc           <  1:1088802/150‑1 (MQ=255)
                                                                                                                                             cGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCAcc          <  2:654804/150‑1 (MQ=255)
                                                                                                                                               gCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGa        <  2:952881/150‑1 (MQ=255)
                                                                                                                                                     gCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGATGcaca  <  1:1728214/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACCAATTGAAGCGATGGCCAACGCAACCAACGCCACCGGCAGTGACCAGACGGAAACCCAGAAGAACAGCAGATAACCGCAGGTTCCCAGCAAAGCGCCAATCAGGAAGGTATTCTTTTTACCGATCCTCGCGACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCTGACCGCAAAGGTCGAAATCAGCACACACAGCGCACCGATGCACA  >  NC_000913/1693268‑1693566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑