Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,702,500 C→G 5.5% H90D (CAC→GAC)  add → adenosine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,702,5000CG 5.5% 312.1 / 6.3 91H90D (CAC→GAC) addadenosine deaminase
Reads supporting (aligned to +/- strand):  ref base C (40/46);  new base G (3/2);  total (43/48)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGC  >  NC_000913/1702352‑1702646
                                                                                                                                                    |                                                                                                                                                  
ggAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTTACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCa                                                                                                                                                   <  2:1144117/150‑1 (MQ=255)
 gAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCATCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCAc                                                                                                                                                  <  2:576507/150‑1 (MQ=255)
   aaCACTGCTTCCCCACGTTCCTGTCATTGCCAACGACCCCGATCTGGTGAGCGTTCTGACCAAACTTTACTGGTGCGTTAAAGTTCTCGCCTCTCTAGATGACTGTCGCCGCGTGGCATTTGAAAACATTGAATATGCAGCCCGTCACgg                                                                                                                                                <  2:321339/150‑1 (MQ=255)
    acacTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGc                                                                                                                                               >  2:744290/1‑150 (MQ=255)
    acacTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGc                                                                                                                                               <  2:1573145/150‑1 (MQ=255)
     cacTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGcc                                                                                                                                              <  2:999194/150‑1 (MQ=255)
     cacTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGcc                                                                                                                                              >  1:1630213/1‑150 (MQ=255)
          aTTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCAc                                                                                                                                         <  1:1470025/150‑1 (MQ=255)
          aTTCCCCACGTGCAGGTCATGGCCAACGAACCCGATCTGGTTAGCTTTCAGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCAc                                                                                                                                         <  2:921169/150‑1 (MQ=255)
           ttCCCCACGTTCATGGCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACAAAACTTGACTGGGGCGTTAAAGTTCTCGCCGCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACt                                                                                                                                        <  2:931067/150‑1 (MQ=255)
           ttCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTTTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACt                                                                                                                                        <  1:450196/150‑1 (MQ=255)
            tCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGTCCGTGACGGGCTGCatct                                                                                                                                       <  1:922773/150‑4 (MQ=255)
             ccccACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTAGCCGCGTGGCATTTGAAAACATTGAAGATTCAGCCCGTCACGGCCTGCACTAt                                                                                                                                      >  2:217021/1‑150 (MQ=255)
              cccAGGTTCAGGTCTTTGCCAACGACCCCGATCTGGTGAGCGTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGACTGTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGGCCGTGTCGGGCTGCatcttc                                                                                                                                     <  2:270998/150‑6 (MQ=255)
               ccACGTGCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTTGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGt                                                                                                                                    <  2:369499/150‑1 (MQ=255)
                        ggTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCg                                                                                                                           <  1:435920/150‑1 (MQ=255)
                        ggTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCg                                                                                                                           <  1:467687/150‑1 (MQ=255)
                        ggTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCg                                                                                                                           >  1:307313/1‑150 (MQ=255)
                        ggTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCg                                                                                                                           <  1:1546015/150‑1 (MQ=255)
                           cATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGttt                                                                                                                        >  2:679489/1‑150 (MQ=255)
                            aTTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGtttt                                                                                                                       <  1:1216996/150‑1 (MQ=255)
                             ttGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCGGCGTGGGATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTAGGTCGAGCTGCGttttt                                                                                                                      >  2:1381770/1‑150 (MQ=255)
                              tGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTc                                                                                                                     >  2:385626/1‑150 (MQ=255)
                              tGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTc                                                                                                                     >  2:392000/1‑150 (MQ=255)
                               gCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTct                                                                                                                    <  1:1390167/150‑2 (MQ=255)
                               gCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCa                                                                                                                    >  1:625814/1‑150 (MQ=255)
                                ccAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCAc                                                                                                                   >  1:222330/1‑150 (MQ=255)
                                ccAACGAACCCGATCTGCTGAGCTTTCTGACCAAACTTGACTGGCGCGTTAAAGATCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGGCACGGCCTGCACTATGTCGAGCTGCGTTTTTCAc                                                                                                                   <  1:151115/150‑1 (MQ=255)
                                 cAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCAcc                                                                                                                  >  2:281160/1‑150 (MQ=255)
                                     gAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGc                                                                                                              <  2:1345958/150‑1 (MQ=255)
                                     gAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATCCAGCCCGTCACGGCCTCCACAATGTCGAGCTGCGTTTTTCACCAGGc                                                                                                              >  2:273066/1‑150 (MQ=255)
                                         ccGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACa                                                                                                          >  1:485726/1‑150 (MQ=255)
                                                 gTGAGCTTTCTGACCAAACTTGACTGCGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCCCGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatggcaatg                                                                                                  <  1:151832/150‑1 (MQ=255)
                                                  tgggCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCACGCCTCTCGTGATGCCTGTCTCCGCGTGGCATTTGAAAGAATTGAAGATGCAGCCCGTCGAGGCCTGCCCTATGTCGAGCTGCGTGTTTCACCAGGCTACatggccatgg                                                                                                 >  1:335818/4‑150 (MQ=255)
                                                  tGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCATGCTACatggcaatgg                                                                                                 >  1:734227/1‑150 (MQ=255)
                                                   gAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatggcaatggc                                                                                                <  2:1280531/150‑1 (MQ=255)
                                                   gAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatggcaatggc                                                                                                <  2:1490550/150‑1 (MQ=255)
                                                   gAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatggcaatggc                                                                                                <  1:744290/150‑1 (MQ=255)
                                                    aGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTCGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatggcaatggca                                                                                               <  2:773216/150‑1 (MQ=255)
                                                     gCTTTCTGACCAAACTTGACTGGGTCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGGATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGcac                                                                                              <  1:767621/150‑1 (MQ=255)
                                                     gCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGcac                                                                                              <  1:966373/150‑1 (MQ=255)
                                                         tCTGATCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCa                                                                                          <  2:1063188/150‑1 (MQ=255)
                                                         acTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCa                                                                                          <  1:1065470/149‑1 (MQ=255)
                                                            gACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCt                                                                                       >  1:1320096/1‑150 (MQ=255)
                                                             aCCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCCGCTg                                                                                      >  2:541782/1‑150 (MQ=255)
                                                              ccAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGc                                                                                     >  2:1424144/1‑150 (MQ=255)
                                                               cAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGcc                                                                                    <  1:644542/150‑1 (MQ=255)
                                                                        cTGGGGCGTTAAAGTTCTAGACTCTCTTGATGCCTGTCGCCTCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCggg                                                                           <  2:1443747/150‑1 (MQ=255)
                                                                         tGGGGCGTTTAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGTCTACTTGGCTATGGCACATCAGCTGCCTGTAGCGGgt                                                                          <  2:1633835/150‑1 (MQ=255)
                                                                          ggggCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGgtg                                                                         <  2:585938/150‑1 (MQ=255)
                                                                             gCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGtgttg                                                                      <  2:1664421/150‑1 (MQ=255)
                                                                               ggtAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTc                                                                    <  1:587756/148‑1 (MQ=255)
                                                                               gTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTc                                                                    >  1:1148285/1‑150 (MQ=255)
                                                                               gTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTc                                                                    <  2:1320096/150‑1 (MQ=255)
                                                                               gTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTc                                                                    >  2:1200596/1‑150 (MQ=255)
                                                                                ttAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCAGTTGAACACATTGATGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCAAATCAGCTGCCTGTAGCGGGTGTTGTCg                                                                   >  1:262979/1‑150 (MQ=255)
                                                                                  aaaGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCCCGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAAAGGCACATCAGCTGCCTGTAGCAGGTGTTCTCGaa                                                                 >  2:496987/1‑150 (MQ=255)
                                                                                  aaaGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGaa                                                                 >  2:1352671/1‑150 (MQ=255)
                                                                                          cGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGAt                                                         <  1:281160/150‑1 (MQ=255)
                                                                                           gCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATc                                                        >  2:1121349/1‑150 (MQ=255)
                                                                                           gCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATc                                                        <  1:379155/150‑1 (MQ=255)
                                                                                            ccTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCg                                                       >  1:363379/1‑150 (MQ=255)
                                                                                             ctctctTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGa                                                      >  2:751040/1‑150 (MQ=255)
                                                                                             ctctctTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGa                                                      >  2:744651/1‑150 (MQ=255)
                                                                                             ctctctTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGa                                                      >  2:501764/1‑150 (MQ=255)
                                                                                               ctctTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATg                                                    <  2:485726/150‑1 (MQ=255)
                                                                                                 ctTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGc                                                  <  2:625814/150‑1 (MQ=255)
                                                                                                  gtGATGCCTGTCGCCGCGTGGGATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCg                                                 <  2:900260/149‑1 (MQ=255)
                                                                                                           gTCGCCGCGTGGCATTTGAAAGCATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAg                                        <  2:617987/150‑1 (MQ=255)
                                                                                                           gTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAg                                        >  2:566366/1‑150 (MQ=255)
                                                                                                              gccgcGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGtt                                     <  2:307313/150‑1 (MQ=255)
                                                                                                                      gCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCAcc                             <  1:1411442/150‑1 (MQ=255)
                                                                                                                        aTTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCtt                           >  1:1691423/1‑150 (MQ=255)
                                                                                                                        aTTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACAAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCtt                           >  2:328371/1‑150 (MQ=255)
                                                                                                                           tGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTgg                        >  1:266075/1‑150 (MQ=255)
                                                                                                                           tGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTgg                        >  1:1431023/1‑150 (MQ=255)
                                                                                                                             aaaaCATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGgtg                      >  2:1340878/1‑150 (MQ=255)
                                                                                                                             aaaaCATTGAAGATGCAGCCCGGCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGgtg                      <  2:1704264/150‑1 (MQ=255)
                                                                                                                               aaCATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGtgtg                    <  2:1148285/150‑1 (MQ=255)
                                                                                                                                    tGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTAGTGTGCAGGc               <  1:351796/150‑1 (MQ=255)
                                                                                                                                    tGAAGATGCAGCCCGTCACGGCCTGCACTATGGCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGGGTTGTCGAAGCGGTGATCGATGGCGGACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGc               <  2:1240083/150‑1 (MQ=255)
                                                                                                                                     gAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCg              >  2:559278/1‑150 (MQ=255)
                                                                                                                                     gAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCg              >  2:1362854/1‑150 (MQ=255)
                                                                                                                                       tagtGCAGGCCGTGACGGGCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGaa            >  1:928320/4‑150 (MQ=255)
                                                                                                                                         gtGCAGGCCGTGACGGGCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGc          >  1:1595465/2‑150 (MQ=255)
                                                                                                                                         gtGCAGGCCGTGACGGGCTGCACTATGTCAAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCGCATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGc          >  1:165317/2‑150 (MQ=255)
                                                                                                                                           gCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCtt        >  1:952835/1‑150 (MQ=255)
                                                                                                                                            cAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTTCGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTa       >  2:232349/1‑150 (MQ=255)
                                                                                                                                            cAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTa       >  2:135159/1‑150 (MQ=255)
                                                                                                                                            cAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTa       >  2:43530/1‑150 (MQ=255)
                                                                                                                                                 cGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGc  <  1:679489/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTATCGGC  >  NC_000913/1702352‑1702646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: