Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,707,044 T→G 14.2% R42R (CGT→CGG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,707,0440TG14.2% 156.9 / 3.3 92R42R (CGT→CGGrsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base T (32/45);  new base G (8/7);  total (40/52)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGGTACCGTTACGGCT  >  NC_000913/1706896‑1707191
                                                                                                                                                    |                                                                                                                                                   
gCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGtt                                                                                                                                                    >  1:1619209/1‑150 (MQ=255)
   tATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTg                                                                                                                                                 <  1:759342/150‑1 (MQ=255)
   tATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAAACCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTg                                                                                                                                                 >  1:260112/1‑150 (MQ=255)
    aTCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGAACCCCTGGCGCAGCGTTTTGt                                                                                                                                                <  1:1115486/150‑1 (MQ=255)
       aTTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTAt                                                                                                                                             >  2:554794/1‑150 (MQ=255)
          cccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTcc                                                                                                                                          <  2:1286844/150‑1 (MQ=255)
          cccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTcc                                                                                                                                          <  1:1244422/150‑1 (MQ=255)
          cccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTcc                                                                                                                                          >  2:1484700/1‑150 (MQ=255)
          cccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTcc                                                                                                                                          <  2:922066/150‑1 (MQ=255)
           ccGTGGTACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTAAACCCCTGGGCCAGGGGACCCGGGCGCAGCGGGTTGTTTTTTCa                                                                                                                                         >  2:1442153/1‑150 (MQ=255)
            cGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGATAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGATAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCAc                                                                                                                                        <  1:1592995/150‑1 (MQ=255)
            cGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCAc                                                                                                                                        <  1:1631816/150‑1 (MQ=255)
               ggAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGTGCAGCGTTTTGTTATTCCACTGa                                                                                                                                     >  2:62426/1‑150 (MQ=255)
                    acCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCCCAGCGTTTTGTTATTCCACTGAAACAg                                                                                                                                >  2:580440/1‑150 (MQ=255)
                     ccATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGc                                                                                                                               >  1:621312/1‑150 (MQ=255)
                      cATGCTTAAGTTCATCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCa                                                                                                                              <  2:255798/150‑1 (MQ=255)
                        tGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCata                                                                                                                            <  2:637121/150‑1 (MQ=255)
                          cTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGTTTTGTTATTACACTTCAACAGCATAtt                                                                                                                          >  1:1374920/1‑150 (MQ=255)
                            tAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTgg                                                                                                                        <  2:13680/150‑1 (MQ=255)
                                ttATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgcgcg                                                                                                                    >  1:1514300/1‑149 (MQ=255)
                                 tATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTg                                                                                                                   <  2:1553306/150‑1 (MQ=255)
                                 tATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTg                                                                                                                   <  2:1608200/150‑1 (MQ=255)
                                 tATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTGTGTTATTCCACTGAAACAGCATATTGGCGCTg                                                                                                                   >  2:853792/1‑150 (MQ=255)
                                  aTTCTCTGCATTCAGAAATAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCAAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCCGCGGTTTGTTATTCCACTGAAACCGCATATTGGCGCTGa                                                                                                                  >  2:1120500/1‑150 (MQ=255)
                                    tctcTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAg                                                                                                                >  1:1404928/1‑150 (MQ=255)
                                    tctcTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAg                                                                                                                <  1:1561484/150‑1 (MQ=255)
                                     ctctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCCGCGTTTTGTTTTTCCACTGAAACAGCATATTGGCGCGGAAgg                                                                                                               >  2:1286309/1‑150 (MQ=255)
                                     ctctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAgg                                                                                                               <  1:1878/150‑1 (MQ=255)
                                      tctGCCTTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGt                                                                                                              <  2:794458/150‑1 (MQ=255)
                                       ctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTg                                                                                                             <  1:452940/150‑1 (MQ=255)
                                         gCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAg                                                                                                           <  1:559466/150‑1 (MQ=255)
                                         gCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTGATTCCACTGTAACAGCATATTGGCGCTGAAGGTGAg                                                                                                           >  1:933403/1‑150 (MQ=255)
                                          cATTCAGAAAAAATAATATCTGGGATTTGAACGGCGGGATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACGCCTGCGCCATGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGt                                                                                                          <  2:763264/150‑1 (MQ=255)
                                              cAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtgtg                                                                                                      >  2:1388253/1‑150 (MQ=255)
                                               aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGc                                                                                                     >  1:892902/1‑150 (MQ=255)
                                                gAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTTCCCCTGGCGCAGCGTTTTGTTATTGCACTGAAACAGCAGATTGGCGCTGAAGGTGAGGTGTGCg                                                                                                    >  2:1104063/1‑150 (MQ=255)
                                                    aaataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTCTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAg                                                                                                <  2:1093579/150‑1 (MQ=255)
                                                       taaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGt                                                                                             >  2:271736/1‑150 (MQ=255)
                                                           atCTGGGATTTCAACGGCGGCATCCATCCTCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGc                                                                                         <  2:355506/150‑1 (MQ=255)
                                                            tCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCg                                                                                        >  1:633371/1‑150 (MQ=255)
                                                                   tttCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGt                                                                                 <  1:1484700/150‑1 (MQ=255)
                                                                     tCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGTGTTGTGCGTTAGCGTCGGCGATAAATTAt                                                                               <  2:31738/150‑1 (MQ=255)
                                                                      cAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGGACCCCAGGCGCCGCGGGTTGTTTTTCCACAGAAACAGCATATTGGCGCGGAAGGGGAGTTGTGCGTTAGCGTCGGCGGTaaaagatt                                                                              >  1:1623700/1‑145 (MQ=255)
                                                                       aaCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTg                                                                             <  1:479483/150‑1 (MQ=255)
                                                                          ggcggaATCCATCCACCGGAGATCAAACCCCAGTCCAACGGCACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTATGTTATTCCACTGAAACAGCATATTCGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgcgc                                                                          <  2:128361/150‑1 (MQ=255)
                                                                           gcggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGcgcg                                                                         <  2:543276/150‑1 (MQ=255)
                                                                           gcggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGcgcg                                                                         <  1:1310217/150‑1 (MQ=255)
                                                                            cggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCgg                                                                        >  2:26240/1‑150 (MQ=255)
                                                                             ggcATCCATCCCCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTAATCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGc                                                                       <  1:1349938/150‑1 (MQ=255)
                                                                             ggcAGCCTTCCACCGGGGATGAAAACCCAGGCCTACGGTACACCCCTGCGCCAGTGACCCCTGGCGCAGCGTCTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGGGAGTTGTGCGATAGCGTCGGCGATAAAGTATTGCGCGGc                                                                       <  2:124043/150‑1 (MQ=255)
                                                                              gcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCGGGCGCGGCGGTTTGTGTTTGCGCGGGGACAGCATATTGGCGGTGAAGGTGAGGTGTGCGTTAGCGTCGGCGATAAAGTTTTGCGCGGcc                                                                      >  1:330245/1‑150 (MQ=255)
                                                                                  ccatccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGGTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGcc                                                                  >  2:192638/1‑150 (MQ=255)
                                                                                    atccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCACGTACCCCAGGCGCAGCGTTTTGTTATTCCACAGAAACAGCATATTTGCGCTGAAGGTGAGTTGTGCGTTAACGTCCGCCATAAAGTAATGCGCCGCCAGcacc                                                                >  2:1415684/1‑147 (MQ=255)
                                                                                        aCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTAc                                                            <  2:892902/150‑1 (MQ=255)
                                                                                           ggAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTgg                                                                                                                        >  2:940469/1‑87 (MQ=255)
                                                                                           ggAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTgg                                                                                                                        <  1:940469/87‑1 (MQ=255)
                                                                                            tagaTTAAAACCCAGTCAAACGGTAAACCCCTGCTCAAGGTACCCCTCGCGCAGCGTTTTGTTATTCCACTGACACAGCATATTGGCGCTGAAGGTGAGTTGTGAGTTATCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGt                                                        <  1:1523852/149‑1 (MQ=255)
                                                                                             agaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCACTGGCGCAGCGTTTTGTTAATCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTATCGTCGGCGATAAAGTATTGAGCGGCCAGCCGCTTACCCGtt                                                       >  2:133033/1‑149 (MQ=255)
                                                                                              gaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTACACTGAAACAGCATATTGGCGCTGAAGGTGAGGTGTGGGTTAGGGTCGGCGATAAAGTATTTCGCGGCCAGCCGCTTACCCgggg                                                      >  1:395542/1‑150 (MQ=255)
                                                                                                tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTc                                                    >  1:1245540/1‑150 (MQ=255)
                                                                                                   aaaCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTTGAGCTGAAGGTGAGTTGTGCGTTAACGTAGGCGATAATCTATTGAGCGGCCAGCCGCTTACCCGTGGTcgcg                                                 >  2:438511/1‑150 (MQ=255)
                                                                                                    aaCCCAGTCCTACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTGTTGTTATTCCACTGCAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCgg                                                <  2:893732/150‑1 (MQ=255)
                                                                                                     tcccAGTCCAACGGTACACTCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGc                                               <  2:159555/149‑1 (MQ=255)
                                                                                                     aCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGc                                               <  2:644087/150‑1 (MQ=255)
                                                                                                     aCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGc                                               >  2:938241/1‑150 (MQ=255)
                                                                                                        cAGTCCAACGGTACACCCCTGCCCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAAAAGCATATTGGCGCTGAAGGTGAGTTGTGGGTTAGCGTCGGCGTTAAAGTATTGAGCGGCCAGCCGCTTGCCCCGGGTCGCGGCaaa                                            >  2:871385/1‑150 (MQ=255)
                                                                                                            ccAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgc                                        >  1:974943/1‑150 (MQ=255)
                                                                                                            ccAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGGTTTTGTATTCCCCGGAAACAGCATATTGGCGATGAACGTGAGGTGGGCGTTAGCGGCGGCAATAAAGTTTTGCTCCGCCAGCCCCATAGCCGGTGTCGGGGCaacaagc                                        >  2:1623450/1‑145 (MQ=255)
                                                                                                            ccAACGGCACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgc                                        <  2:1555079/150‑1 (MQ=255)
                                                                                                              aaCGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgctg                                      <  2:560347/150‑1 (MQ=255)
                                                                                                               ccGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGGTATTCCACTGAAACAGCATATTGGCGCTGAAGGGGAGTTGGGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgctgc                                     <  1:1092815/149‑1 (MQ=255)
                                                                                                                cGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGcc                                    <  1:1286309/150‑1 (MQ=255)
                                                                                                                  gTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTg                                  <  2:1615537/150‑1 (MQ=255)
                                                                                                                  gTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTg                                  <  2:633371/150‑1 (MQ=255)
                                                                                                                     cacCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTc                               <  2:36477/150‑1 (MQ=255)
                                                                                                                       ccccTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAc                             <  2:621312/150‑1 (MQ=255)
                                                                                                                       ccccTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAc                             >  2:1594363/1‑150 (MQ=255)
                                                                                                                        cgcTGCGCCAGGGGTACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcg                            <  2:669757/148‑1 (MQ=255)
                                                                                                                         gcTGCGCCAGGGGTACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcgc                           >  1:118601/2‑150 (MQ=255)
                                                                                                                           tGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACgcgc                         <  2:920757/150‑1 (MQ=255)
                                                                                                                               ccAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTTCGCGTCCAGCCGCTTACCCGTGGTCGCGGCAAAATGATGCCTGTTCACGCGCCCAc                     >  1:1597601/1‑150 (MQ=255)
                                                                                                                                cAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCAcc                    <  2:1544642/150‑1 (MQ=255)
                                                                                                                                   gTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCg                 <  1:1442153/150‑1 (MQ=255)
                                                                                                                                    tACCCCTGGGGCAGCGTTTTGGTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCgg                <  1:62426/150‑1 (MQ=255)
                                                                                                                                     aCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCggg               <  2:1404928/150‑1 (MQ=255)
                                                                                                                                      ccccTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGGt              <  1:679866/150‑1 (MQ=255)
                                                                                                                                       cccTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGGTa             >  2:1271924/1‑150 (MQ=255)
                                                                                                                                         cTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAATGCTGCCTGTTCACGCGCCCACCTCGGGTACCg          <  2:1134607/150‑1 (MQ=255)
                                                                                                                                               cAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGGTACCGTTACg     <  1:524286/150‑1 (MQ=255)
                                                                                                                                                 gCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGGTACCGTTACGGc   >  1:1455958/1‑150 (MQ=255)
                                                                                                                                                 gCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGGTACCGTTACGGc   >  2:306539/1‑150 (MQ=255)
                                                                                                                                                 gCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGGTACCGTTACGGCt  >  1:1660100/1‑150 (MQ=255)
                                                                                                                                                  cGTTTTGTTAATCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGGTACCGTTACGGCt  <  1:759868/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGGTACCGTTACGGCT  >  NC_000913/1706896‑1707191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: