| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,708,844 | C→G | 28.1% | A642A (GCC→GCG) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,708,844 | 0 | C | G | 28.1% | 5.5 / 6.6 | 21 | A642A (GCC→GCG) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base C (12/2); new base G (3/3); total (15/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01 | |||||||||||
AGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGC > NC_000913/1708723‑1708993 | aGCTGGAACAGCAACAGGCTAATGAGGAACCAGAAGAACAGGTCGATCCGCGCAACGCCGCCGTCCACGCCGCGATTGACCCGGCCACAGCCCGCAAGCGGGAGGGGCAACACACTAAGGCCGGGCGCGAACGTCAGGTCGGTCCGCGCa > 2:689804/1‑150 (MQ=9) cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGAGCAAAAGCACTCAAGCTCGAACAGCAACAGGATAATGACTAGACAGAAGAACAGGTGGATCCGCGCAAAGCCGCAGta > 2:561763/1‑149 (MQ=11) aCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCGGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTAGATCCGAGCAAAGCAGCCGTCGCAGCCGCTATTGcccc > 1:1725348/1‑149 (MQ=11) cAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTGGAAGCGGCTATTGCCCGAGCCAAAGCCCGCAACCAGGACCAGCACCAGGCTATTGCCGTGGCAGAAGAAGAGGTCGATCAGCGCACAGCCCCATTTGACGCGGCTAGTGccccgg > 2:353689/1‑147 (MQ=2) gaagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGCCGCCGGTATTGCCCGAGCCAAGGCCCGCAAGCGGGCACAGCACCAGGCTGATGCCGAGCCAGAACAACTGGTCGGTCCGCGCAACGCCGCCGTAGCAGCGGCTAATGCCCGCGcc > 1:741464/1‑150 (MQ=14) gaagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTCGATCAGCGCAAAGCCGCGGTAGACGCGGCGATTGCCCGCGcc > 1:977737/1‑150 (MQ=11) aaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCAAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTGGATCCGCGCAAAGCTGCAGTAGAAGCCGCTATTGCAAGAGCAAAAg > 2:1412892/1‑150 (MQ=11) gcAAAGCCGCCCTCGAAGCCGCTATAGACCGCACCAAATCACCCAATCTGGAACAGGAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAAc < 1:1646700/150‑1 (MQ=17) ccgccgccGTCGTAGCGGCTATTGCCCGTGCCGAAGCCCGCAAGCTGGAAGAGCAACAGGCTCATGCCGAGCCAGAACACCAGGTCGATCCGCGCAAATCCGCCGGCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGc < 1:274420/148‑1 (MQ=255) aGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGCGCTATCCCCCCAGACCCGCCGTCGAACCCGCATATGCCGGCGCGGAAGCCCGCAACAGGGaaaaaca > 1:1618873/1‑145 (MQ=2) cTATTGCCCGTTCCACAGCACGCAAGCTCTAACAGCAACAGGCTAATTCAGATCCAGAAGACCCTTTAGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGc < 1:544633/150‑1 (MQ=14) gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTAGCCGCGGCTATTGCCCGCGCAACCGCGCGAAAACGCGCACAGCAGCAGGCTGAAGCGGAGccctaa > 1:1208976/1‑146 (MQ=14) aaGCCCGCAATATGTAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCTATCTGCTAAAATCCTCCGGCGTAGCCGCTATTGCCCGAGCCAAAGCGCGCAATCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTg < 2:1568298/150‑1 (MQ=11) cAAGCTGGAACAGCAACAGGCTAATGCGGATCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAAc < 1:1726236/107‑1 (MQ=255) cAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAAc > 2:1726236/1‑107 (MQ=12) cAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGCGCAAAATCGCTCAACCGTCAACATCAAAAGACTAATGAGGAGACTAAAGAACAGTTTTATCcgaa > 2:666627/1‑148 (MQ=9) aGCTGGAACAGCAACAGGCTAATGCGGTACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCGAACGCGCGCAAACCGGAACAGCAGCCGGCTAATGCGGAGCCCGAACATCAGGTGGATCAGCGCa > 1:364951/1‑150 (MQ=11) gCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGc < 1:73137/105‑1 (MQ=255) gCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGc > 2:73137/1‑105 (MQ=255) cTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCGGCCGTCGACGCgg > 2:1500492/1‑150 (MQ=255) gCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGATCAAACGCGCAAACCAGCCATCGAAGCGGCTAta > 1:498512/1‑149 (MQ=14) cGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAATCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGc < 2:571847/150‑1 (MQ=255) | AGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGC > NC_000913/1708723‑1708993 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |