Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,778,364 C→T 5.6% intergenic (+17/+26) ydiO → / ← ydiP putative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,778,3640CT 5.6% 331.0 / 5.3 107intergenic (+17/+26)ydiO/ydiPputative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (58/43);  new base T (4/2);  total (62/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01

Only 100 of 113 total aligned reads displayed.

GGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGG  >  NC_000913/1778216‑1778511
                                                                                                                                                    |                                                                                                                                                      
ggCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGct                                                                                                                                                       >  2:404523/1‑150 (MQ=255)
ggCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGct                                                                                                                                                       <  1:1269623/150‑1 (MQ=255)
ggCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGct                                                                                                                                                       <  2:546621/150‑1 (MQ=255)
ggCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGCCGGCGCAGct                                                                                                                                                       >  1:1397745/1‑150 (MQ=255)
   tataCCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGcttct                                                                                                                                                    <  2:181828/150‑1 (MQ=255)
    ataCCGTTGAGGCGCGAGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATTATCTGCAGGCGGCGCAGcttctt                                                                                                                                                   <  1:935584/150‑1 (MQ=255)
     taCCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTa                                                                                                                                                  <  1:1580040/150‑1 (MQ=255)
      aCCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGCACGTAACGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCGGAAAGACTATCAGAACAAATCATCTGCAGGCGGCGCAGCTTCTTaa                                                                                                                                                 <  1:81909/150‑1 (MQ=255)
       ccGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAAc                                                                                                                                                >  2:1626324/1‑150 (MQ=255)
         gATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGCCGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGCAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCGTCTTAActa                                                                                                                                              >  1:394573/1‑148 (MQ=255)
                cgcgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGGCTCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTgcgc                                                                                                                                       <  1:641359/150‑1 (MQ=255)
                cgcgcgTCTCCCGCTTCTGGCGTGATGTCCGGTGTGAACGTATAGGCGGCGTTACAGACGAAATTATGATTTCCGTAGCAGGGCGGCAGATCCTGAAAGAATATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTtcgc                                                                                                                                       <  2:1517415/150‑1 (MQ=255)
                 gcgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGcgcc                                                                                                                                      <  1:413572/150‑1 (MQ=255)
                  cgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGCCTTTCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTTACAAACTGcgccg                                                                                                                                     >  1:1642368/1‑150 (MQ=255)
                  cgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgccg                                                                                                                                     >  1:1507216/1‑150 (MQ=255)
                    cgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCgccgc‑‑‑c                                                                                                                                <  2:1102145/150‑1 (MQ=255)
                        tcCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTAAGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGAt                                                                                                                            >  1:661787/1‑150 (MQ=255)
                             ctgcTGGCGTGATGTGCGTTGTGAACGTCTCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATc                                                                                                                       <  1:153003/147‑1 (MQ=255)
                             cTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATc                                                                                                                       >  1:527571/1‑150 (MQ=255)
                              ttCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATcc                                                                                                                      <  2:1731589/150‑1 (MQ=255)
                                 tGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCcaac                                                                                                                   >  2:68950/1‑150 (MQ=255)
                                    cGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAg                                                                                                                >  2:1636658/1‑150 (MQ=255)
                                      ggATGTCTTTTGTGAACGTATCTGCGGCGTTTCAGAAGAAATTATGATTTTCGTAGCAGGTCGGCAGATCGTGAAAGACTATCAGAACAAATATTCTGCAGGCGGCGCAGCTTCTTAACAATCTGCTCCTC‑‑‑CGGATTTATCCAACAAGAc                                                                                                              <  2:1134029/149‑1 (MQ=255)
                                       tttGTCCGTTGTTAACTATTCGGCGGCGGAACATACTAAATTATTATTTAGTTAGCCGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACt                                                                                                             <  1:128543/148‑1 (MQ=255)
                                        aTGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACtt                                                                                                            >  2:1654975/1‑150 (MQ=255)
                                             cGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCgg                                                                                                       <  1:1185976/150‑1 (MQ=255)
                                              gTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGt                                                                                                      <  1:404523/150‑1 (MQ=255)
                                              gTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGt                                                                                                      >  2:1208062/1‑150 (MQ=255)
                                               ttGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGtt                                                                                                     <  2:534071/150‑1 (MQ=255)
                                               ttGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGtt                                                                                                     >  2:1590338/1‑150 (MQ=255)
                                                tgtgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTg                                                                                                    <  2:1023985/150‑1 (MQ=255)
                                                 gtgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGa                                                                                                   <  2:451153/150‑1 (MQ=255)
                                                 gtgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGa                                                                                                   >  2:256021/1‑150 (MQ=255)
                                                  tgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAg                                                                                                  >  1:1345355/1‑150 (MQ=255)
                                                    aaCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGCCTTAGCGGTTGAGGa                                                                                                >  1:466972/1‑150 (MQ=255)
                                                     aCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGaa                                                                                               <  2:1210913/150‑1 (MQ=255)
                                                      cGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATGTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGaaa                                                                                              <  1:68950/150‑1 (MQ=255)
                                                      cGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGaaa                                                                                              >  2:1426130/1‑150 (MQ=255)
                                                       gTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGAGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAAt                                                                                             <  1:233898/150‑1 (MQ=255)
                                                           cggcggcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAg                                                                                         >  1:1457814/1‑150 (MQ=255)
                                                             gcggctgTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGAt                                                                                       <  2:438652/150‑1 (MQ=255)
                                                               ggcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAg                                                                                     <  1:787497/150‑1 (MQ=255)
                                                                gctgTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAgg                                                                                    <  2:682067/147‑1 (MQ=255)
                                                                 cggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGa                                                                                   >  1:1492355/1‑150 (MQ=255)
                                                                  ggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGAt                                                                                  <  2:1718273/150‑1 (MQ=255)
                                                                     aCAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTc                                                                               <  1:256021/150‑1 (MQ=255)
                                                                         aCGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGt                                                                           >  2:363011/1‑150 (MQ=255)
                                                                         aCGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGAATTCTTGt                                                                           >  1:226610/1‑150 (MQ=255)
                                                                          cGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAAAAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTgttgtt                                                                          >  2:1565426/1‑150 (MQ=255)
                                                                           gAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTc                                                                         >  2:782645/1‑150 (MQ=255)
                                                                            aaaTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTcc                                                                        <  1:610203/150‑1 (MQ=255)
                                                                              aTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGc                                                                      <  1:864503/150‑1 (MQ=255)
                                                                                   gATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAAc                                                                 <  1:1594208/150‑1 (MQ=255)
                                                                                    aTTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACa                                                                >  2:1125430/1‑150 (MQ=255)
                                                                                    aTTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGAGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑AAGATTTATCCAAAAAGACTTACCGGTTGAGGAAATTAAGATATGATTTATTGTTACGCAAAACa                                                                >  1:548609/1‑150 (MQ=255)
                                                                                       tACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtt                                                             >  2:1236502/1‑150 (MQ=255)
                                                                                       tACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtt                                                             >  2:1495919/1‑150 (MQ=255)
                                                                                       tACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGAAAGGATTTCTTGTTCCGCAAAACAGtt                                                             >  2:490771/1‑150 (MQ=255)
                                                                                          gTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt                                                          >  1:1233803/1‑150 (MQ=255)
                                                                                            aGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaa                                                        >  2:110516/1‑150 (MQ=255)
                                                                                            aGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaa                                                        <  2:1507216/150‑1 (MQ=255)
                                                                                            aGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaa                                                        >  2:1386966/1‑150 (MQ=255)
                                                                                             gCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaaa                                                       <  1:1626324/150‑1 (MQ=255)
                                                                                                ggTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTg                                                    <  1:817926/150‑1 (MQ=255)
                                                                                                  tCGGCAGAGCCTGAAAGCCTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTTACAAACGGCGCCGC‑‑‑CAGGTTTATCCAACAAGCGTTAGCGGTTGAGGAATTTCAGATAGGATTTCTTGTTCCGGAATAGAGTTGTTAAATTggc                                                  >  2:660022/1‑148 (MQ=255)
                                                                                                   cGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCt                                                 >  1:1038315/1‑150 (MQ=255)
                                                                                                   cGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCt                                                 <  2:1475583/150‑1 (MQ=255)
                                                                                                      cAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACg                                              >  1:1165991/1‑150 (MQ=255)
                                                                                                       aGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACgg                                             <  1:1506820/150‑1 (MQ=255)
                                                                                                       aGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACgg                                             >  2:1147991/1‑150 (MQ=255)
                                                                                                         aTCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTa                                           <  1:1636658/150‑1 (MQ=255)
                                                                                                            cTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATc                                        <  1:1613121/150‑1 (MQ=255)
                                                                                                             tGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCg                                       <  1:1387319/150‑1 (MQ=255)
                                                                                                              gAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGc                                      <  1:1654975/150‑1 (MQ=255)
                                                                                                              gAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGc                                      >  2:1664684/1‑150 (MQ=255)
                                                                                                                aaGCCTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTg                                    >  1:957910/1‑150 (MQ=255)
                                                                                                                      aTCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGaa                              >  1:767720/1‑150 (MQ=255)
                                                                                                                        cAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGaaca                            >  2:243158/1‑150 (MQ=255)
                                                                                                                         aGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTAACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGaacac                           >  1:362220/1‑149 (MQ=255)
                                                                                                                            aCAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCAACGGTAATCGCTGGGCGAaccaccc                        >  2:1423012/1‑149 (MQ=255)
                                                                                                                             cAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGa                       >  1:1399336/1‑150 (MQ=255)
                                                                                                                             cAAATAATCTGCAGGCGGCGCAGCTTCATAACAAACTGAGCCGC‑‑‑CAGAATTATACAAAAAGACTTACCGGTTGAGGAAATTAAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTTCGGTTATAGCTGGGCGAACAAACGa                       >  1:1503031/1‑150 (MQ=255)
                                                                                                                              aaaTAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGAATTACCGGTTTAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGACCAACCGAc                      >  2:1099164/1‑150 (MQ=255)
                                                                                                                                ataatCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACAt                    >  2:512804/1‑150 (MQ=255)
                                                                                                                                   atCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgc                 <  1:1236502/150‑1 (MQ=255)
                                                                                                                                    tCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgcg                <  2:970724/150‑1 (MQ=255)
                                                                                                                                    aaatctggcggcGCAGTTTGTTAAGAAGCTGCGCCGCCTGCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACACCCGACATg                   >  2:1020189/7‑150 (MQ=37)
                                                                                                                                     cTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACCACAGACATGgcgc               >  1:1040649/1‑150 (MQ=255)
                                                                                                                                     cTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgcgc               >  2:1374469/1‑150 (MQ=255)
                                                                                                                                       gCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCaa             >  1:434402/1‑150 (MQ=255)
                                                                                                                                        ctggcggcGCAGTTTGTTAAGAAGCTGCGCCGC‑‑‑CTGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGAGAACAACCGACATGGCGCaaa            >  1:1177858/3‑150 (MQ=39)
                                                                                                                                        cAGGCGGCGCAGCTTCTTAACATACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTAACGGTTGAGGAAATTCAGAAAGGATTTCTTGTTCCGCAAAACAGGTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATCGCGCaaa            >  2:770999/1‑150 (MQ=255)
                                                                                                                                         aGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCaaaa           >  1:1512854/1‑150 (MQ=255)
                                                                                                                                           gcggcgCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAAc         <  2:1457814/150‑1 (MQ=255)
                                                                                                                                             ggcgCAGTTTGTTAAGAAGCTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgc                 <  1:622380/140‑1 (MQ=255)
                                                                                                                                             ggcgCAGTTTGTTAAGAAGCTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgc                 >  2:622380/1‑140 (MQ=255)
                                                                                                                                             ggcgCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAg       >  1:1040179/1‑150 (MQ=255)
                                                                                                                                                gcAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGttt    <  1:1660597/150‑1 (MQ=255)
                                                                                                                                                  aGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTgg  <  1:1202885/150‑1 (MQ=255)
                                                                                                                                                  aGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTgg  <  1:1138411/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
GGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGG  >  NC_000913/1778216‑1778511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: