Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,778,372 C→G 5.5% intergenic (+25/+18) ydiO → / ← ydiP putative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,778,3720CG 5.5% 348.6 / 9.2 109intergenic (+25/+18)ydiO/ydiPputative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (59/43);  new base G (4/2);  total (64/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.00e-01

Only 100 of 113 total aligned reads displayed.

CCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATC  >  NC_000913/1778223‑1778521
                                                                                                                                                     |                                                                                                                                                         
  gATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGCCGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGCAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCGTCTTAActa                                                                                                                                                         >  1:394573/1‑148 (MQ=255)
         cgcgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGGCTCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTgcgc                                                                                                                                                  <  1:641359/150‑1 (MQ=255)
         cgcgcgTCTCCCGCTTCTGGCGTGATGTCCGGTGTGAACGTATAGGCGGCGTTACAGACGAAATTATGATTTCCGTAGCAGGGCGGCAGATCCTGAAAGAATATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTtcgc                                                                                                                                                  <  2:1517415/150‑1 (MQ=255)
          gcgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGTTTGTTAAGAAGCTGcgcc                                                                                                                                                 <  1:413572/150‑1 (MQ=255)
           cgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGCCTTTCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTTACAAACTGcgccg                                                                                                                                                >  1:1642368/1‑150 (MQ=255)
           cgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgccg                                                                                                                                                >  1:1507216/1‑150 (MQ=255)
             cgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCgccgc‑‑‑c                                                                                                                                           <  2:1102145/150‑1 (MQ=255)
                 tcCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTAAGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGAt                                                                                                                                       >  1:661787/1‑150 (MQ=255)
                      ctgcTGGCGTGATGTGCGTTGTGAACGTCTCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATc                                                                                                                                  <  1:153003/147‑1 (MQ=255)
                      cTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATc                                                                                                                                  >  1:527571/1‑150 (MQ=255)
                       ttCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATcc                                                                                                                                 <  2:1731589/150‑1 (MQ=255)
                       ttCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATcc                                                                                                                                 <  1:1702373/150‑1 (MQ=255)
                          tGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCcaac                                                                                                                              >  2:68950/1‑150 (MQ=255)
                             cGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAg                                                                                                                           >  2:1636658/1‑150 (MQ=255)
                               ggATGTCTTTTGTGAACGTATCTGCGGCGTTTCAGAAGAAATTATGATTTTCGTAGCAGGTCGGCAGATCGTGAAAGACTATCAGAACAAATATTCTGCAGGCGGCGCAGCTTCTTAACAATCTGCTCCTC‑‑‑CGGATTTATCCAACAAGAc                                                                                                                         <  2:1134029/149‑1 (MQ=255)
                                tttGTCCGTTGTTAACTATTCGGCGGCGGAACATACTAAATTATTATTTAGTTAGCCGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACt                                                                                                                        <  1:128543/148‑1 (MQ=255)
                                 aTGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACtt                                                                                                                       >  2:1654975/1‑150 (MQ=255)
                                      cGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCgg                                                                                                                  <  1:1185976/150‑1 (MQ=255)
                                       gTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGt                                                                                                                 >  2:1208062/1‑150 (MQ=255)
                                       gTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGt                                                                                                                 <  1:404523/150‑1 (MQ=255)
                                        ttGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGtt                                                                                                                >  2:1590338/1‑150 (MQ=255)
                                        ttGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGtt                                                                                                                <  2:534071/150‑1 (MQ=255)
                                         tgtgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTg                                                                                                               <  2:1023985/150‑1 (MQ=255)
                                          gtgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGa                                                                                                              <  2:451153/150‑1 (MQ=255)
                                          gtgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGa                                                                                                              >  2:256021/1‑150 (MQ=255)
                                           tgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAg                                                                                                             >  1:758571/1‑150 (MQ=255)
                                           tgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAg                                                                                                             >  1:1345355/1‑150 (MQ=255)
                                             aaCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGCCTTAGCGGTTGAGGa                                                                                                           >  1:466972/1‑150 (MQ=255)
                                              aCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGaa                                                                                                          <  2:1210913/150‑1 (MQ=255)
                                               cGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGaaa                                                                                                         >  2:1426130/1‑150 (MQ=255)
                                                gTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGAGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAAt                                                                                                        <  1:233898/150‑1 (MQ=255)
                                                     ggcggcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGa                                                                                                   >  2:548768/1‑150 (MQ=255)
                                                      gcggctgTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGAt                                                                                                  <  2:438652/150‑1 (MQ=255)
                                                        ggcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAg                                                                                                <  1:787497/150‑1 (MQ=255)
                                                         gctgTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAgg                                                                                               <  2:682067/147‑1 (MQ=255)
                                                          cggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGa                                                                                              >  1:1492355/1‑150 (MQ=255)
                                                           ggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGAt                                                                                             <  2:1718273/150‑1 (MQ=255)
                                                              aCAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTc                                                                                          <  1:256021/150‑1 (MQ=255)
                                                                  aCGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGt                                                                                      >  2:363011/1‑150 (MQ=255)
                                                                   cGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAAAAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTgttgtt                                                                                     >  2:1565426/1‑150 (MQ=255)
                                                                    gAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTc                                                                                    >  2:782645/1‑150 (MQ=255)
                                                                    gAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGACGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGtta                                                                                    >  1:464068/1‑149 (MQ=255)
                                                                     aaaTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTcc                                                                                   <  1:610203/150‑1 (MQ=255)
                                                                       aTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGc                                                                                 <  1:864503/150‑1 (MQ=255)
                                                                             aTTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACa                                                                           >  2:1125430/1‑150 (MQ=255)
                                                                             aTTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGAGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑AAGATTTATCCAAAAAGACTTACCGGTTGAGGAAATTAAGATATGATTTATTGTTACGCAAAACa                                                                           >  1:548609/1‑150 (MQ=255)
                                                                                tACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtt                                                                       >  2:1236502/1‑150 (MQ=255)
                                                                                tACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtt                                                                       >  2:1495919/1‑150 (MQ=255)
                                                                                tACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGAAAGGATTTCTTGTTCCGCAAAACAGtt                                                                       >  2:490771/1‑150 (MQ=255)
                                                                                   gTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt                                                                    >  1:1233803/1‑150 (MQ=255)
                                                                                     aGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaa                                                                  <  2:1507216/150‑1 (MQ=255)
                                                                                     aGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaa                                                                  >  2:1386966/1‑150 (MQ=255)
                                                                                     aGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaa                                                                  >  2:110516/1‑150 (MQ=255)
                                                                                      gCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaaa                                                                 <  1:1626324/150‑1 (MQ=255)
                                                                                         ggTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTg                                                              <  1:817926/150‑1 (MQ=255)
                                                                                            cGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCt                                                           >  1:1038315/1‑150 (MQ=255)
                                                                                            cGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCt                                                           <  2:1475583/150‑1 (MQ=255)
                                                                                               cAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACg                                                        >  1:1165991/1‑150 (MQ=255)
                                                                                                aGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACgg                                                       <  1:1506820/150‑1 (MQ=255)
                                                                                                aGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACgg                                                       >  2:1147991/1‑150 (MQ=255)
                                                                                                  aTCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTa                                                     <  1:1636658/150‑1 (MQ=255)
                                                                                                     cTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATc                                                  <  1:1613121/150‑1 (MQ=255)
                                                                                                      tGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCg                                                 <  1:1387319/150‑1 (MQ=255)
                                                                                                       gAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGc                                                >  2:1664684/1‑150 (MQ=255)
                                                                                                       gAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGc                                                <  1:1654975/150‑1 (MQ=255)
                                                                                                         aaGCCTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTg                                              >  1:957910/1‑150 (MQ=255)
                                                                                                               aTCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGaa                                        >  1:767720/1‑150 (MQ=255)
                                                                                                                 cAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGaaca                                      >  2:243158/1‑150 (MQ=255)
                                                                                                                  aGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTAACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGaacac                                     >  1:362220/1‑149 (MQ=255)
                                                                                                                     aCAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCAACGGTAATCGCTGGGCGAaccaccc                                  >  2:1423012/1‑149 (MQ=255)
                                                                                                                      cAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGa                                 >  1:1399336/1‑150 (MQ=255)
                                                                                                                      cAAATAATCTGCAGGCGGCGCAGCTTCATAACAAACTGAGCCGC‑‑‑CAGAATTATACAAAAAGACTTACCGGTTGAGGAAATTAAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTTCGGTTATAGCTGGGCGAACAAACGa                                 >  1:1503031/1‑150 (MQ=255)
                                                                                                                       aaaTAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGAATTACCGGTTTAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGACCAACCGAc                                >  2:1099164/1‑150 (MQ=255)
                                                                                                                         ataatCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACAt                              >  2:512804/1‑150 (MQ=255)
                                                                                                                          taatCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATg                             <  1:1590338/150‑1 (MQ=255)
                                                                                                                            atCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgc                           <  1:1236502/150‑1 (MQ=255)
                                                                                                                             tCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgcg                          <  2:970724/150‑1 (MQ=255)
                                                                                                                             aaatctggcggcGCAGTTTGTTAAGAAGCTGCGCCGCCTGCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACACCCGACATg                             >  2:1020189/7‑150 (MQ=37)
                                                                                                                              cTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACCACAGACATGgcgc                         >  1:1040649/1‑150 (MQ=255)
                                                                                                                              cTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgcgc                         >  2:1374469/1‑150 (MQ=255)
                                                                                                                                 ctggcggcGCAGTTTGTTAAGAAGCTGCGCCGC‑‑‑CTGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGAGAACAACCGACATGGCGCaaa                      >  1:1177858/3‑150 (MQ=39)
                                                                                                                                 cAGGCGGCGCAGCTTCTTAACATACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTAACGGTTGAGGAAATTCAGAAAGGATTTCTTGTTCCGCAAAACAGGTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATCGCGCaaa                      >  2:770999/1‑150 (MQ=255)
                                                                                                                                  aGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCaaaa                     >  1:1512854/1‑150 (MQ=255)
                                                                                                                                    gcggcgCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAAc                   <  2:1457814/150‑1 (MQ=255)
                                                                                                                                      ggcgCAGTTTGTTAAGAAGCTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgc                           <  1:622380/140‑1 (MQ=255)
                                                                                                                                      ggcgCAGTTTGTTAAGAAGCTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgc                           >  2:622380/1‑140 (MQ=255)
                                                                                                                                      ggcgCAGTTTGTTAAGAAGCTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAg                 >  1:1402024/1‑150 (MQ=255)
                                                                                                                                      ggcgCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAg                 >  1:1040179/1‑150 (MQ=255)
                                                                                                                                        cgcAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGtt               >  2:1634228/1‑150 (MQ=255)
                                                                                                                                         gcAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGttt              <  1:1660597/150‑1 (MQ=255)
                                                                                                                                           aGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTgg            <  1:1202885/150‑1 (MQ=255)
                                                                                                                                           aGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTgg            <  1:1138411/150‑1 (MQ=255)
                                                                                                                                               tcttAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATAGCTGGGCGAACAGCCGACATGGCTCAAAAACAGAATGGCaaa        >  1:342700/1‑150 (MQ=255)
                                                                                                                                                cttAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACACCCGACATGGCGCAAAAACAGTTTGGCaaa        >  2:851099/1‑150 (MQ=255)
                                                                                                                                                 ttAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGt      <  2:1492355/150‑1 (MQ=255)
                                                                                                                                                  tAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGAGATACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTg     >  2:867232/1‑150 (MQ=255)
                                                                                                                                                  tAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTg     >  1:1322335/1‑150 (MQ=255)
                                                                                                                                                  tAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATACATATAGGAGTTCATGTTCCGCAAAAAAGTTTTTAAATTCTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGAAAAAACAGTTTGGCAAAGTg     <  1:274380/150‑1 (MQ=255)
                                                                                                                                                   aaCAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGa    >  1:892617/1‑150 (MQ=255)
                                                                                                                                                     cAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATc  <  1:1495919/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                         
CCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGC‑‑‑CAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATC  >  NC_000913/1778223‑1778521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: