Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,778,375 A→G 5.5% intergenic (+28/+15) ydiO → / ← ydiP putative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,778,3750AG 5.5% 328.0 / 6.8 110intergenic (+28/+15)ydiO/ydiPputative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (60/43);  new base G (4/2);  total (64/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

Only 100 of 111 total aligned reads displayed.

CGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCG  >  NC_000913/1778232‑1778522
                                                                                                                                               |                                                                                                                                                    
cgcgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGGCTCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTgcgc                                                                                                                                                <  1:641359/150‑1 (MQ=255)
cgcgcgTCTCCCGCTTCTGGCGTGATGTCCGGTGTGAACGTATAGGCGGCGTTACAGACGAAATTATGATTTCCGTAGCAGGGCGGCAGATCCTGAAAGAATATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTtcgc                                                                                                                                                <  2:1517415/150‑1 (MQ=255)
 gcgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgcc                                                                                                                                               >  2:1202885/1‑150 (MQ=255)
  cgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGCCTTTCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTTACAAACTGcgccg                                                                                                                                              >  1:1642368/1‑150 (MQ=255)
  cgcgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGcgccg                                                                                                                                              >  1:1507216/1‑150 (MQ=255)
    cgTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCgccgcc                                                                                                                                            <  2:1102145/150‑1 (MQ=255)
             ctgcTGGCGTGATGTGCGTTGTGAACGTCTCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATc                                                                                                                                   <  1:153003/147‑1 (MQ=255)
             cTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATc                                                                                                                                   >  1:527571/1‑150 (MQ=255)
              ttCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATcc                                                                                                                                  <  1:1702373/150‑1 (MQ=255)
                 tGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCcaac                                                                                                                               >  2:68950/1‑150 (MQ=255)
                    cGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAg                                                                                                                            >  2:1636658/1‑150 (MQ=255)
                      ggATGTCTTTTGTGAACGTATCTGCGGCGTTTCAGAAGAAATTATGATTTTCGTAGCAGGTCGGCAGATCGTGAAAGACTATCAGAACAAATATTCTGCAGGCGGCGCAGCTTCTTAACAATCTGCTCCTCCGGATTTATCCAACAAGAc                                                                                                                          <  2:1134029/149‑1 (MQ=255)
                        aTGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACtt                                                                                                                        >  2:1654975/1‑150 (MQ=255)
                             cGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCgg                                                                                                                   <  1:1185976/150‑1 (MQ=255)
                              gTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGt                                                                                                                  >  2:1208062/1‑150 (MQ=255)
                              gTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGt                                                                                                                  <  1:404523/150‑1 (MQ=255)
                               ttGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGtt                                                                                                                 <  2:534071/150‑1 (MQ=255)
                               ttGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGtt                                                                                                                 >  2:1590338/1‑150 (MQ=255)
                                tgtgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTg                                                                                                                <  2:1023985/150‑1 (MQ=255)
                                 gtgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGa                                                                                                               >  2:256021/1‑150 (MQ=255)
                                 gtgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGa                                                                                                               <  2:451153/150‑1 (MQ=255)
                                  tgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAg                                                                                                              >  1:1345355/1‑150 (MQ=255)
                                  tgAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAg                                                                                                              >  1:758571/1‑150 (MQ=255)
                                    aaCGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGCCTTAGCGGTTGAGGa                                                                                                            >  1:466972/1‑150 (MQ=255)
                                      cGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATGTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGaaa                                                                                                          <  1:68950/150‑1 (MQ=255)
                                      cGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGaaa                                                                                                          >  2:1426130/1‑150 (MQ=255)
                                       gTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGAGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAAt                                                                                                         <  1:233898/150‑1 (MQ=255)
                                           cggcggcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAg                                                                                                     >  1:1457814/1‑150 (MQ=255)
                                             gcggctgTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGAt                                                                                                   <  2:438652/150‑1 (MQ=255)
                                               ggcggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAg                                                                                                 <  1:787497/150‑1 (MQ=255)
                                                gctgTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAgg                                                                                                <  2:682067/147‑1 (MQ=255)
                                                gcggTTCAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAATGACTATCAGATCAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAgg                                                                                                <  2:341928/150‑1 (MQ=255)
                                                 cggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGa                                                                                               >  1:1492355/1‑150 (MQ=255)
                                                  ggTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGAt                                                                                              <  2:1718273/150‑1 (MQ=255)
                                                     aCAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTc                                                                                           <  1:256021/150‑1 (MQ=255)
                                                         aCGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGt                                                                                       >  2:363011/1‑150 (MQ=255)
                                                         aCGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGAATTCTTGt                                                                                       >  1:226610/1‑150 (MQ=255)
                                                          cGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAAAAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTgttgtt                                                                                      >  2:1565426/1‑150 (MQ=255)
                                                           gAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTc                                                                                     >  2:782645/1‑150 (MQ=255)
                                                           gAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGACGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGtta                                                                                     >  1:464068/1‑149 (MQ=255)
                                                            aaaTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTcc                                                                                    <  1:610203/150‑1 (MQ=255)
                                                              aTTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGc                                                                                  <  1:864503/150‑1 (MQ=255)
                                                                   gATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAAc                                                                             <  1:1594208/150‑1 (MQ=255)
                                                                    aTTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACa                                                                            >  2:1125430/1‑150 (MQ=255)
                                                                    aTTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGAGGCGCAGCTTCTTAACAAACTGCGCCGCAAGATTTATCCAAAAAGACTTACCGGTTGAGGAAATTAAGATATGATTTATTGTTACGCAAAACa                                                                            >  1:548609/1‑150 (MQ=255)
                                                                       tACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGtt                                                                        >  2:1236502/1‑150 (MQ=255)
                                                                       tACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGAAAGGATTTCTTGTTCCGCAAAACAGtt                                                                        >  2:490771/1‑150 (MQ=255)
                                                                          gTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGttttt                                                                     >  1:1233803/1‑150 (MQ=255)
                                                                            aGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaa                                                                   <  2:1507216/150‑1 (MQ=255)
                                                                            aGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaa                                                                   >  2:110516/1‑150 (MQ=255)
                                                                            aGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaa                                                                   >  2:1386966/1‑150 (MQ=255)
                                                                             gCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTaaa                                                                  <  1:1626324/150‑1 (MQ=255)
                                                                                ggTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTg                                                               <  1:817926/150‑1 (MQ=255)
                                                                                  tCGGCAGAGCCTGAAAGCCTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTTACAAACGGCGCCGCCAGGTTTATCCAACAAGCGTTAGCGGTTGAGGAATTTCAGATAGGATTTCTTGTTCCGGAATAGAGTTGTTAAATTggc                                                             >  2:660022/1‑148 (MQ=255)
                                                                                   cGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCt                                                            <  2:1475583/150‑1 (MQ=255)
                                                                                   cGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCt                                                            >  1:1038315/1‑150 (MQ=255)
                                                                                      cAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACg                                                         >  1:1165991/1‑150 (MQ=255)
                                                                                       aGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACgg                                                        >  2:1147991/1‑150 (MQ=255)
                                                                                         aTCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTa                                                      <  1:1636658/150‑1 (MQ=255)
                                                                                            cTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATc                                                   <  1:1613121/150‑1 (MQ=255)
                                                                                             tGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCg                                                  <  1:1387319/150‑1 (MQ=255)
                                                                                              gAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGc                                                 >  2:1664684/1‑150 (MQ=255)
                                                                                                aaGCCTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTg                                               >  1:957910/1‑150 (MQ=255)
                                                                                                      aTCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGaa                                         >  1:767720/1‑150 (MQ=255)
                                                                                                        cAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGaaca                                       >  2:243158/1‑150 (MQ=255)
                                                                                                         aGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTAACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGaacac                                      >  1:362220/1‑149 (MQ=255)
                                                                                                            aCAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCAACGGTAATCGCTGGGCGAaccaccc                                   >  2:1423012/1‑149 (MQ=255)
                                                                                                             cAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGa                                  >  1:1399336/1‑150 (MQ=255)
                                                                                                             cAAATAATCTGCAGGCGGCGCAGCTTCATAACAAACTGAGCCGCCAGAATTATACAAAAAGACTTACCGGTTGAGGAAATTAAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTTCGGTTATAGCTGGGCGAACAAACGa                                  >  1:1503031/1‑150 (MQ=255)
                                                                                                              aaaTAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGAATTACCGGTTTAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGACCAACCGAc                                 >  2:1099164/1‑150 (MQ=255)
                                                                                                                ataatCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACAt                               >  2:512804/1‑150 (MQ=255)
                                                                                                                 taatCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATg                              <  1:1590338/150‑1 (MQ=255)
                                                                                                                   atCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgc                            <  1:1236502/150‑1 (MQ=255)
                                                                                                                   atCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgc                            >  1:963595/1‑150 (MQ=255)
                                                                                                                    tCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgcg                           <  2:970724/150‑1 (MQ=255)
                                                                                                                     cTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACCACAGACATGgcgc                          >  1:1040649/1‑150 (MQ=255)
                                                                                                                     cTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgcgc                          >  2:1374469/1‑150 (MQ=255)
                                                                                                                       gCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCaa                        >  1:434402/1‑150 (MQ=255)
                                                                                                                        ctggcggcGCAGTTTGTTAAGAAGCTGCGCCGCCTGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGAGAACAACCGACATGGCGCaaa                       >  1:1177858/3‑150 (MQ=39)
                                                                                                                        cAGGCGGCGCAGCTTCTTAACATACTGCGCCGCCAGATTTATCCAACAAGACTTAACGGTTGAGGAAATTCAGAAAGGATTTCTTGTTCCGCAAAACAGGTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATCGCGCaaa                       >  2:770999/1‑150 (MQ=255)
                                                                                                                         aGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCaaaa                      >  1:1512854/1‑150 (MQ=255)
                                                                                                                           gcggcgCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAAc                    <  2:1457814/150‑1 (MQ=255)
                                                                                                                            cggcgCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACAGACATGGCGCAAAAACa                   >  2:1475008/1‑150 (MQ=255)
                                                                                                                             ggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgc                            >  2:622380/1‑140 (MQ=255)
                                                                                                                             ggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGgc                            <  1:622380/140‑1 (MQ=255)
                                                                                                                             ggcgCAGTTTGTTAAGAAGCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAg                  >  1:1402024/1‑150 (MQ=255)
                                                                                                                             ggcgCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAg                  >  1:1040179/1‑150 (MQ=255)
                                                                                                                               cgcAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGtt                >  2:1634228/1‑150 (MQ=255)
                                                                                                                                gcAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGttt               <  1:1660597/150‑1 (MQ=255)
                                                                                                                                  aGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTgg             <  1:1202885/150‑1 (MQ=255)
                                                                                                                                  aGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTgg             <  1:1138411/150‑1 (MQ=255)
                                                                                                                                      tcttAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATAGCTGGGCGAACAGCCGACATGGCTCAAAAACAGAATGGCaaa         >  1:342700/1‑150 (MQ=255)
                                                                                                                                       cttAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACACCCGACATGGCGCAAAAACAGTTTGGCaaa         >  2:851099/1‑150 (MQ=255)
                                                                                                                                        ttAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGt       <  2:1492355/150‑1 (MQ=255)
                                                                                                                                         tAACAAACTGCGCCGCCAGATTTATCCAACAAGAGATACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTg      >  2:867232/1‑150 (MQ=255)
                                                                                                                                         tAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTg      >  1:1322335/1‑150 (MQ=255)
                                                                                                                                         tAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATACATATAGGAGTTCATGTTCCGCAAAAAAGTTTTTAAATTCTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGAAAAAACAGTTTGGCAAAGTg      <  1:274380/150‑1 (MQ=255)
                                                                                                                                          aaCAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGa     >  1:892617/1‑150 (MQ=255)
                                                                                                                                            cAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATc   <  1:1495919/150‑1 (MQ=255)
                                                                                                                                             aaaCTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCg  <  1:548768/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CGCGCGTCTCCCGCTTCTGGCGTGATGTCCGTTGTGAACGTATCGGCGGCGGTACAGACGAAATTATGATTTACGTAGCAGGTCGGCAGATCCTGAAAGACTATCAGAACAAATAATCTGCAGGCGGCGCAGCTTCTTAACAAACTGCGCCGCCAGATTTATCCAACAAGACTTACCGGTTGAGGAAATTCAGATAGGATTTCTTGTTCCGCAAAACAGTTTTTAAATTGTCTACGGTAATCGCTGGGCGAACAACCGACATGGCGCAAAAACAGTTTGGCAAAGTGATCG  >  NC_000913/1778232‑1778522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: