Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,792,240 T→A 8.7% intergenic (‑220/+27) ydiV ← / ← nlpC anti‑FlhD4C2 factor, inactive EAL family phosphodiesterase/putative C40 clan peptidase lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,792,2400TA 8.7% 281.2 / 12.0 102intergenic (‑220/+27)ydiV/nlpCanti‑FlhD4C2 factor, inactive EAL family phosphodiesterase/putative C40 clan peptidase lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (41/52);  new base A (2/7);  total (43/59)
Fisher's exact test for biased strand distribution p-value = 2.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTAC  >  NC_000913/1792091‑1792386
                                                                                                                                                     |                                                                                                                                                  
ttACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGt                                                                                                                                                    <  1:1305218/150‑1 (MQ=255)
 tACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTAATGTATTCAGGAGTAAATGGACGGCGATGTa                                                                                                                                                   >  1:590121/1‑150 (MQ=255)
 tACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACATTTTCAGGGGTGCTTTTTTTAGATTATCCTGATTATAAACGAATATTCCATTTACGGAATTTTTGTCTGCAAAATACTAATGTATTCAGGAGTAAATGGACGGCGAGGTa                                                                                                                                                   >  1:857010/1‑150 (MQ=255)
  aCAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTat                                                                                                                                                  >  1:1707139/1‑150 (MQ=255)
  aCAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTat                                                                                                                                                  <  1:1236428/150‑1 (MQ=255)
     gAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGTACGGCGATGTatcat                                                                                                                                               >  2:1397475/1‑150 (MQ=255)
       agtattAACTTTCATTTGTTACATGTTAACCACTTTTTAATGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATTATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCg                                                                                                                                             <  1:742856/145‑1 (MQ=255)
       aGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTCTCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCg                                                                                                                                             <  2:85535/150‑1 (MQ=255)
       aGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACTAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCg                                                                                                                                             >  2:695855/1‑150 (MQ=255)
        ggAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGc                                                                                                                                            <  2:793310/150‑1 (MQ=255)
         gAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGcc                                                                                                                                           <  1:143860/150‑1 (MQ=255)
          aGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGATACATCGCCg                                                                                                                                          <  2:1372559/150‑1 (MQ=255)
           gTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCCGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGt                                                                                                                                         <  2:592430/150‑1 (MQ=255)
            tAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATTAACGAATAATCGATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGATACATCGCCGTc                                                                                                                                        <  1:706861/150‑1 (MQ=255)
            tAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTc                                                                                                                                        <  1:391809/150‑1 (MQ=255)
            tAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGATACATCGCCGTc                                                                                                                                        <  2:1424653/150‑1 (MQ=255)
            tAACTTTCATTTGTTCCATGTTAACCACTTTATCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTCCTGTATTCAGGAGTAAATGGACGGCGATGATACATCGCCGTc                                                                                                                                        <  2:1204521/150‑1 (MQ=255)
             aaCTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTcc                                                                                                                                       <  2:738821/150‑1 (MQ=255)
             aaCTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGATACATCGCCGTcc                                                                                                                                       <  1:900914/150‑1 (MQ=255)
              aCTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCa                                                                                                                                      <  2:1697514/150‑1 (MQ=255)
              aCTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGATACATCGCCGTCCa                                                                                                                                      <  2:896445/150‑1 (MQ=255)
               cTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCAt                                                                                                                                     >  2:569365/1‑150 (MQ=255)
               atttCATAAGTTCCATGTTAACCACTTTTTCAGGGGTCCTATTTTTAGATAATCCTGATTATAAACGAATAATCCATTTACGGAATTTTAGACTGCATAAAACTAGTGTATTAAGGAGTAAATGCACGGCGATGTATCATCGACGTCCAt                                                                                                                                     >  2:1260621/2‑150 (MQ=255)
                     tttGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCg                                                                                                                               <  2:78647/150‑1 (MQ=255)
                     tttGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCg                                                                                                                               <  1:1115740/150‑1 (MQ=255)
                        gTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACa                                                                                                                            >  2:74779/1‑150 (MQ=255)
                           ccATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATAc                                                                                                                         >  2:523903/1‑150 (MQ=255)
                            cATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACt                                                                                                                        <  1:1565912/150‑1 (MQ=255)
                            cATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGAATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACt                                                                                                                        <  1:1265701/150‑1 (MQ=255)
                             atcttAAACACTTTTTCAGTCGTCTTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACTGAATAATTGTCTGCAAAATACTACTGTATTCAGGAGAAAATGTACGCCGATGTATCATCGCCGTCCATTTTTCGACATACTa                                                                                                                       <  1:84011/147‑1 (MQ=255)
                             aTGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGTGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTa                                                                                                                       >  2:1191367/1‑150 (MQ=255)
                                ttAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGAAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGAt                                                                                                                    <  2:1431594/150‑1 (MQ=255)
                                   aCCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCg                                                                                                                 <  1:421058/150‑1 (MQ=255)
                                     cACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGtc                                                                                                               <  2:1056584/150‑1 (MQ=255)
                                      aCTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGtct                                                                                                              <  2:95119/150‑1 (MQ=255)
                                             cAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTAATGAATTTTTGTCTGCAAAATACTACTGTATTAAGGAGTAAATGGACGGCGATGTATCATCGCAGTAAATTTTTCTACATACTAGATTCGTCTAGCCTGc                                                                                                       >  1:63692/1‑150 (MQ=255)
                                                       ttttttAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAtttt                                                                                             <  1:1495077/150‑1 (MQ=255)
                                                       ttttttAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGGCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAtttt                                                                                             <  2:8119/150‑1 (MQ=255)
                                                          tttAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCt                                                                                          <  2:622477/150‑1 (MQ=255)
                                                          tttAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCt                                                                                          >  1:839509/1‑150 (MQ=255)
                                                          tttAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCt                                                                                          <  2:1707139/150‑1 (MQ=255)
                                                            tAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAAATTTCTgg                                                                                        <  1:778719/150‑2 (MQ=255)
                                                             tgATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGcc                                                                                       <  2:387530/149‑1 (MQ=255)
                                                              gATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCa                                                                                      >  2:623271/1‑150 (MQ=255)
                                                                ttATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGCCGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAAt                                                                                    <  1:936193/150‑1 (MQ=255)
                                                                ttATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGTCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCTCCTGCCAGAAATTTTTCTGCCaaa                                                                                    >  1:783605/1‑149 (MQ=255)
                                                                 tATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATa                                                                                   >  1:1249562/1‑150 (MQ=255)
                                                                 tATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAAATTTCTGGCaggc                                                                                   <  1:902977/150‑4 (MQ=255)
                                                                       gATTATAAACGAATAATCCCTTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACAtt                                                                             <  2:1109406/150‑1 (MQ=255)
                                                                           ataAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCa                                                                         <  1:287119/150‑1 (MQ=255)
                                                                           ataAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCa                                                                         <  1:523903/150‑1 (MQ=255)
                                                                            taAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCaa                                                                        >  2:1406833/1‑150 (MQ=255)
                                                                            taAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCaa                                                                        >  2:79691/1‑150 (MQ=255)
                                                                              aaCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGt                                                                      <  2:782894/150‑1 (MQ=255)
                                                                                 gAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAg                                                                   >  1:22636/1‑150 (MQ=255)
                                                                                 gAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAg                                                                   >  2:734468/1‑150 (MQ=255)
                                                                                  aataatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAgg                                                                  <  1:1145755/150‑1 (MQ=255)
                                                                                     aatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAAc                                                               <  2:596714/150‑1 (MQ=255)
                                                                                     aatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATAATCTCCGTACATTTTTCGACATACTAGATTCGTCTCGCCTGCTAGAAATTTTTCTGCCAATATACATTATCAAGTGAGGAAc                                                               >  1:77120/1‑150 (MQ=255)
                                                                                       tCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGc                                                             <  1:691825/150‑1 (MQ=255)
                                                                                         cATTTACGTAATTTTTGTATGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCAt                                                           <  2:542320/150‑1 (MQ=255)
                                                                                         cATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGGCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCAt                                                           <  1:679498/150‑1 (MQ=255)
                                                                                         aaTTTACGTAATTTTTGTCTGCAAAATACTACTGTATTCATGAGTAAATTGACGGCTATGAATCATCGCCTTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCATAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCAt                                                           <  1:284412/149‑1 (MQ=255)
                                                                                             tACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATAAAGTGAGGAACGCATCACt                                                       >  1:1617957/1‑150 (MQ=255)
                                                                                                    tttttGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGc                                                <  1:28119/150‑1 (MQ=255)
                                                                                                         gTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTa                                           >  1:1067428/1‑150 (MQ=255)
                                                                                                            tGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTagag                                        <  2:968408/150‑1 (MQ=255)
                                                                                                            gtccatttactccTGAATACAGTAGTAAATGGACGGCGATGTAGCAACTCCTTCCATTATTCTAAATAATATATTCGTCACCACTGACAGAAAATTATCTTAAAAAAAAAATTATCAAGTGAGTAACGCATCACTCCCTTGCTGGTagag                                        >  2:607621/8‑150 (MQ=255)
                                                                                                             gCAATATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCAGTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAgg                                       <  1:244025/150‑1 (MQ=255)
                                                                                                             gCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAgg                                       <  2:339768/150‑1 (MQ=255)
                                                                                                             gCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAgg                                       <  1:433531/150‑1 (MQ=255)
                                                                                                               aaaaTACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTAGACATACTAGATTCGTATCGCCTGCCAGAAAATTTTCAGCCAATAGACAATATAAATTGAGGAACCCATCACTCCCTTGCGGGTAGAGGCg                                     >  1:1507537/1‑150 (MQ=255)
                                                                                                                aaaTACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACA‑TATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTg                                   <  1:991648/150‑1 (MQ=255)
                                                                                                                 aaTACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTGCCTTGCTGGTAGAGGCGTg                                   <  2:1665131/150‑1 (MQ=255)
                                                                                                                     ctactGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTTAGGAACGCATCACTCACTTGCTGGTAGAGGCGTGGATa                               >  2:828204/1‑150 (MQ=255)
                                                                                                                     ctactGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATa                               >  1:539616/1‑150 (MQ=255)
                                                                                                                     ctactGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCGTGCTGGTAGAGGCGTGGATa                               >  1:350215/1‑150 (MQ=255)
                                                                                                                     ctactGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACCCATCACTCCCTTGCTGGTAGAGTCGTGGATa                               >  2:334627/1‑150 (MQ=255)
                                                                                                                       actGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATaaa                             >  2:1145482/1‑150 (MQ=255)
                                                                                                                         tGTATTCAGGTGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAAtt                           <  1:1191367/150‑1 (MQ=255)
                                                                                                                          gTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAAttt                          >  2:1233950/1‑149 (MQ=255)
                                                                                                                          gTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTATGCCAATAGACATTATCAAGTGAGGAACGCATCACTACCGTGCTGGTAGAGGCGTGGATAAAttt                          >  1:1442792/1‑149 (MQ=255)
                                                                                                                           tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTAAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTgg                         >  1:515243/1‑150 (MQ=255)
                                                                                                                             ttCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgtt                       >  2:749975/1‑150 (MQ=255)
                                                                                                                             ttCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgtt                       <  1:695855/150‑1 (MQ=255)
                                                                                                                                 ggAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTACCTTGCTGGTAGAGGCGTGGTTAAATTGGttgttg                   >  2:988599/1‑150 (MQ=255)
                                                                                                                                  gAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTgg                  <  1:1082036/150‑1 (MQ=255)
                                                                                                                                    gTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTa                >  2:1598623/1‑150 (MQ=255)
                                                                                                                                    gTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTa                <  1:790896/150‑1 (MQ=255)
                                                                                                                                      aaaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGAAATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCATTGCTGGTATAGGCGTGGATAAATTGGTTGTTGGTATc              >  1:1158384/1‑150 (MQ=255)
                                                                                                                                         tGGACGGCGATGTTTCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCTAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTa           <  2:77120/150‑1 (MQ=255)
                                                                                                                                          ggACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTaa          <  1:623271/150‑1 (MQ=255)
                                                                                                                                          ggACGGCGATGATACATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTaa          >  1:1290630/1‑150 (MQ=255)
                                                                                                                                           gACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTaaa         >  2:185011/1‑150 (MQ=255)
                                                                                                                                            aCGGCGATGATACATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAAt        >  1:1391154/1‑150 (MQ=255)
                                                                                                                                               gCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATAcc     >  2:1315780/1‑150 (MQ=255)
                                                                                                                                               gCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATAcc     <  1:1180729/150‑1 (MQ=255)
                                                                                                                                                 gATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGttgttg                   <  2:1409077/134‑1 (MQ=255)
                                                                                                                                                 gATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGttgttg                   >  1:1409077/1‑134 (MQ=255)
                                                                                                                                                  aTGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTAc  >  1:1609818/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTAC  >  NC_000913/1792091‑1792386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑