Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,810,069 G→A 8.3% intergenic (+21/‑142) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,810,0690GA 8.3% 327.2 / 11.8 108intergenic (+21/‑142)yniC/ydjM2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
Reads supporting (aligned to +/- strand):  ref base G (44/55);  new base A (4/5);  total (48/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01

Only 100 of 108 total aligned reads displayed.

TAAAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCATGA  >  NC_000913/1809922‑1810214
                                                                                                                                                   |                                                                                                                                                 
tAAAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCt                                                                                                                                                 <  1:462875/150‑1 (MQ=255)
    gcagcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGccc                                                                                                                                             <  1:606971/150‑1 (MQ=255)
     ccgcCCGCATGCGTTCCAGCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGcccc                                                                                                                                            <  2:302653/148‑1 (MQ=255)
     cagcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGcccc                                                                                                                                            <  1:1155492/150‑1 (MQ=255)
      agcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCGGccccg                                                                                                                                           >  1:620975/1‑149 (MQ=255)
      agcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGCCCCt                                                                                                                                           <  1:1143990/150‑1 (MQ=255)
      agcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGCCCCt                                                                                                                                           <  1:1014355/150‑1 (MQ=255)
      agcCCGCATGCGTTCCATCGTCGGTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTACTGCCCAGGGGCAGTGATCTCGCTGCCCCt                                                                                                                                           <  1:299304/150‑1 (MQ=255)
      agcCCGCAGGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGAAGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGGTAATGACCAGCGGCAGCGAGATCACTGCCCCt                                                                                                                                           <  1:271608/150‑1 (MQ=255)
       gcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTg                                                                                                                                          >  1:625876/1‑150 (MQ=255)
        cccGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTgg                                                                                                                                         >  1:653400/1‑150 (MQ=255)
         ccGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTGTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGt                                                                                                                                        <  1:531577/150‑1 (MQ=255)
           gCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTc                                                                                                                                      >  2:601402/1‑150 (MQ=255)
             aTGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCtt                                                                                                                                    <  1:138905/150‑1 (MQ=255)
             aTGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGCCCCTGGtcatt                                                                                                                                    <  2:775593/150‑5 (MQ=255)
              tGCGTTCCATCGTCGGTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACCTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCttt                                                                                                                                   <  2:1088179/150‑1 (MQ=255)
                     cATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGaa                                                                                                                            >  1:464194/1‑150 (MQ=255)
                       tcgtcgTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAAt                                                                                                                          <  1:1258430/150‑1 (MQ=255)
                            gtTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGACCTCACCACAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCAt                                                                                                                     <  1:275649/150‑1 (MQ=255)
                              tCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTc                                                                                                                   <  1:422233/150‑1 (MQ=255)
                                 tGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAAc                                                                                                                <  1:1287041/150‑1 (MQ=255)
                                     ccAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCCACTGAc                                                                                                            >  1:98736/1‑150 (MQ=255)
                                      cataatcgcAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCTCCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACg                                                                                                           <  1:866843/144‑1 (MQ=255)
                                      cAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACg                                                                                                           <  1:559217/150‑1 (MQ=255)
                                        gAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGa                                                                                                         <  2:1413158/150‑1 (MQ=255)
                                        gAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGa                                                                                                         >  2:1573162/1‑150 (MQ=255)
                                         aaGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGTTTAATGACCAGGGGCAGTGATCTCGCTTCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGAt                                                                                                        >  1:97347/1‑150 (MQ=255)
                                         aaGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGAt                                                                                                        <  2:653171/150‑1 (MQ=255)
                                             gcAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAAt                                                                                                    <  2:1161730/150‑1 (MQ=255)
                                              cAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATc                                                                                                   <  2:100732/150‑1 (MQ=255)
                                              aaaaaTGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATc                                                                                                   <  2:325436/149‑1 (MQ=255)
                                                 aaTGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTc                                                                                                <  2:218504/150‑1 (MQ=255)
                                                    gATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAAt                                                                                             >  2:1620195/1‑150 (MQ=255)
                                                    gATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGATTAATccgtcagt                                                                                            <  2:1556351/150‑8 (MQ=255)
                                                     aaccACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAAtt                                                                                            <  2:714259/148‑1 (MQ=255)
                                                     aTCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACATTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCACTGGATCTTTATCTGAAATTGAATTCAACTGACGGATTAATCGTCAAtt                                                                                            >  1:336524/1‑150 (MQ=255)
                                                      tCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAAttt                                                                                           >  1:772685/1‑150 (MQ=255)
                                                      tCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAAttt                                                                                           >  1:779229/1‑150 (MQ=255)
                                                      tCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAAttt                                                                                           <  2:776521/150‑1 (MQ=255)
                                                       ccACGTTTTGTATTAGCAGACGTCAAACTTTCTTCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTa                                                                                          <  2:376810/150‑1 (MQ=255)
                                                        cACGTTTTGTATTAGCAGACGTCAAACTTTCAACGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                                                         >  1:47224/1‑150 (MQ=255)
                                                         aCGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAag                                                                                        <  1:1175220/150‑1 (MQ=255)
                                                                gTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCCCCGCATAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAaga                                                                                 >  1:1420401/1‑150 (MQ=255)
                                                                gTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAaga                                                                                 <  2:1378189/150‑1 (MQ=255)
                                                                 tATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAagag                                                                                <  1:1663720/150‑1 (MQ=255)
                                                                 tATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAagag                                                                                <  1:1573162/150‑1 (MQ=255)
                                                                    tAGCAGACGTCAAACTTTCATCGCTGACAGAACTAACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGtgt                                                                             <  2:653400/150‑3 (MQ=255)
                                                                    tAGAAGACGTCAAACTTTCATCGCTGACTGAACTCACAGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTa                                                                             <  1:1065513/150‑1 (MQ=255)
                                                                            gTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACCCCGTcc                                                                     >  2:571163/1‑149 (MQ=255)
                                                                             tCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcac                                                                    <  2:569797/150‑1 (MQ=255)
                                                                              cAAACTTTCATCGCTGACAGAACTCCCCGCAAAAGACCATCTAGGTTAATGACCAGGGGCAGAGATCTCGCTGCCCCTGGTAAAATAGCTGAAAGTGCATACAACTGCCGGATTAAGCGTCAACTTAAGAGATAGACTTCCACCGTcaca                                                                   >  1:286755/1‑149 (MQ=255)
                                                                              cAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcacc                                                                   <  1:975667/150‑1 (MQ=255)
                                                                               aaaCTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcacca                                                                  >  2:237933/1‑150 (MQ=255)
                                                                                   tttCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTc                                                              <  1:1352153/150‑1 (MQ=255)
                                                                                      cATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGt                                                           >  2:770380/1‑150 (MQ=255)
                                                                                      cATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGt                                                           >  2:1544169/1‑150 (MQ=255)
                                                                                         cGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCa                                                        >  1:1651976/1‑150 (MQ=255)
                                                                                          gCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCCCCACTTCCGTGCAc                                                       >  1:873700/1‑150 (MQ=255)
                                                                                          gCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCAc                                                       >  1:1731737/1‑150 (MQ=255)
                                                                                           cTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACt                                                      <  1:532597/150‑1 (MQ=255)
                                                                                             gACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGAGATCACGCTGCCCCTCGTTCTTTATCAGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGCGTTACACCGTCACCACTTCCGTCCACTGt                                                    >  2:1575987/1‑150 (MQ=255)
                                                                                               cAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGtat                                                  <  2:98736/150‑1 (MQ=255)
                                                                                               cAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGATGCCCCTAATTAATTATCTGAAATTGGATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACAACGTCACCACTTCCGTGCACAGtat                                                  >  2:1361939/1‑150 (MQ=255)
                                                                                                aGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACAGtata                                                 >  1:218991/1‑150 (MQ=255)
                                                                                                 gAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGAGCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGCAAGAGTTACACCGTCACCACTTCCGGGCCCTGTAGaa                                                >  2:284220/1‑150 (MQ=255)
                                                                                                  aaCTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAAt                                                                                             >  2:1237832/1‑104 (MQ=255)
                                                                                                  aaCTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAAt                                                                                             <  1:1237832/104‑1 (MQ=255)
                                                                                                  aaCTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAAt                                                                                             <  1:1651029/104‑1 (MQ=255)
                                                                                                  aaCTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAAt                                                                                             >  2:1651029/1‑104 (MQ=255)
                                                                                                    cTCACCGCAAAAGACCTTCTCCGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTTAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaaaa                                             >  2:131590/1‑150 (MQ=255)
                                                                                                      cACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGGTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCATTTTAAGAGAAAGAGTTACACAGTCCCCACTTACGTGGACTGTATAAAAATc                                           >  2:1556603/1‑150 (MQ=255)
                                                                                                       cccGCAAAAGCCCTTCTCGGTTAATGACCACGGGCCGTGCTCTCGCTGCCCCTGGTTCTTTAACTGAACTTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACCCCGTCCCCACTTCCGTGCACTGTATAAAAATcc                                          <  2:711127/149‑1 (MQ=255)
                                                                                                       aCCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGTATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATcc                                          <  1:565471/150‑1 (MQ=255)
                                                                                                       aCCGCAAAAGACCTTCTCGGTTAAAGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGGGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATcc                                          <  2:1714395/150‑1 (MQ=255)
                                                                                                         cGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTTCACCGTCACCACTTCCGTGCACTGTATAAAAATCCta                                        >  2:727792/1‑150 (MQ=255)
                                                                                                          gCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCtat                                       <  2:464194/150‑1 (MQ=255)
                                                                                                            aaaaGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATAc                                     <  2:934061/150‑1 (MQ=255)
                                                                                                                gACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTa                                 <  1:1620195/150‑1 (MQ=255)
                                                                                                                gACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTa                                 >  1:965780/1‑150 (MQ=255)
                                                                                                                 aCCTTCTGGGTTAATGACCAGGGGCAGTGATATCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGTTTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTAc                                <  2:670563/150‑1 (MQ=255)
                                                                                                                  ccTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACg                               >  2:1473626/1‑150 (MQ=255)
                                                                                                                    ttCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACGTCCGTGCACTGTATAATAATCCTATACTGTACGTa                             >  1:481539/1‑150 (MQ=255)
                                                                                                                      ctcGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTTCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATc                           <  1:533774/150‑1 (MQ=255)
                                                                                                                       tcGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCg                          >  1:129314/1‑150 (MQ=255)
                                                                                                                            tAATGCCCAGGGGCCGTGATCTCGCGGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGt                     <  2:265001/150‑1 (MQ=255)
                                                                                                                              aTGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGttt                   <  2:1619412/150‑1 (MQ=255)
                                                                                                                               tGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTa                  >  1:347796/1‑150 (MQ=255)
                                                                                                                                gACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAg                 <  2:1588577/150‑1 (MQ=255)
                                                                                                                                gACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAg                 >  1:619180/1‑150 (MQ=255)
                                                                                                                                gACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAg                 >  1:1521628/1‑150 (MQ=255)
                                                                                                                                 aCCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGt                >  1:641877/1‑150 (MQ=255)
                                                                                                                                    aGGGGCAGCGAGATCACTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAg             >  1:402579/1‑150 (MQ=255)
                                                                                                                                    aGGGGCAGCGAGATCACTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAg             >  1:208604/1‑150 (MQ=255)
                                                                                                                                     ggggCAGCGAGATCACTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGt            >  2:1634296/1‑150 (MQ=255)
                                                                                                                                       ggCAGTGATATCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAATATTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGttt          >  1:798289/1‑150 (MQ=255)
                                                                                                                                           gTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATc      >  2:604853/1‑150 (MQ=255)
                                                                                                                                            tGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGACAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCa     <  2:1077570/150‑1 (MQ=255)
                                                                                                                                            tGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCa     <  1:413775/150‑1 (MQ=255)
                                                                                                                                            tGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCa     <  2:396328/150‑1 (MQ=255)
                                                                                                                                               tctcGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCATGa  >  2:94359/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TAAAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCATGA  >  NC_000913/1809922‑1810214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: