Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,821,241 T→A 6.1% intergenic (‑27/+58) chbC ← / ← chbB N,N'‑diacetylchitobiose‑specific enzyme IIC component of PTS/N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,821,2410TA 6.1% 330.0 / 8.8 115intergenic (‑27/+58)chbC/chbBN,N'‑diacetylchitobiose‑specific enzyme IIC component of PTS/N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS
Reads supporting (aligned to +/- strand):  ref base T (49/59);  new base A (4/3);  total (53/62)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

Only 100 of 118 total aligned reads displayed.

CGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAATTACT  >  NC_000913/1821092‑1821389
                                                                                                                                                     |                                                                                                                                                    
cGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAAtt                                                                                                                                                      >  2:337539/1‑150 (MQ=255)
cGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAAtt                                                                                                                                                      >  2:910802/1‑150 (MQ=255)
     gggTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAat                                                                                                                                                 <  2:16988/150‑1 (MQ=255)
     gggTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAat                                                                                                                                                 <  1:1141419/150‑1 (MQ=255)
      ggTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAata                                                                                                                                                >  1:1423484/1‑150 (MQ=255)
       gTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAatat                                                                                                                                               >  1:559433/1‑150 (MQ=255)
        ttAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGTAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATAtt                                                                                                                                              <  1:201078/150‑1 (MQ=255)
          aaCGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGc                                                                                                                                            <  1:718680/150‑1 (MQ=255)
          aaCGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGc                                                                                                                                            <  2:826350/150‑1 (MQ=255)
          aaCGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGc                                                                                                                                            >  2:302860/1‑150 (MQ=255)
            cGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCgg                                                                                                                                          <  2:1035932/150‑1 (MQ=255)
             ggCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATATTTAAATTGCGGt                                                                                                                                         <  1:1459169/150‑1 (MQ=255)
               cATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTAt                                                                                                                                       >  2:880118/1‑150 (MQ=255)
                aTTAAGCGAATAAAGCCATTTTTGAATGCATTAACGTGTGGCTGCTTTCCTAATTTATCTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTAtt                                                                                                                                      <  2:875183/150‑1 (MQ=255)
                  tAAGCGAATAAAGCTATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGa                                                                                                                                    <  1:1674217/150‑1 (MQ=255)
                   aaGCGAATAAAGCCATTTTTGATTGCATTAACGTGTTGCTGCTTTCCTATTTTAACTGCAAAAGTGAGGAGTACCTTTTCAAGAGATGCAATAACATTACTCATAGAAAAATAACCTTAAAAACAGCAATTTAAATATTGCGGAATTGAt                                                                                                                                   >  1:344334/1‑150 (MQ=255)
                     gCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGAttt                                                                                                                                 >  1:106932/1‑150 (MQ=255)
                      cGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATAACCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTa                                                                                                                                <  2:71871/150‑1 (MQ=255)
                      cGAATAAAGCAATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTa                                                                                                                                <  1:152732/150‑1 (MQ=255)
                       gAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATATTTAAATTGCGGtttttaagggt                                                                                                                               <  1:1447507/150‑8 (MQ=255)
                                 aTTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAActc                                                                                                                     <  2:794708/150‑1 (MQ=255)
                                  tttttGATTGCATAAACGTGTGGCTGCTTTCCTTTTTTAACTGCAATAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAActct                                                                                                                    <  2:738691/150‑1 (MQ=255)
                                  tttttGATTGCAATAACGTTTGTCTGCTTTCATATATTAACTGCAAAAGGGAGTAGTACATTTTCAATCTATTCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTAAAATAATGCGGTATTGATTTATGAAATAActct                                                                                                                    <  1:278882/150‑1 (MQ=255)
                                      tGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGa                                                                                                                >  2:576783/1‑150 (MQ=255)
                                               tAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTAACGGAAAAAt                                                                                                       >  1:920575/1‑150 (MQ=255)
                                                 aCGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAAttt                                                                                                     >  2:1310202/1‑150 (MQ=255)
                                                  cGTGTGGCTGCTTTCCTATTTTAACTGCAAAATGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTa                                                                                                    <  1:1069105/150‑1 (MQ=255)
                                                    tgtgGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGTTGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTaaa                                                                                                  <  1:1343047/150‑1 (MQ=255)
                                                    tgtgGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTaaa                                                                                                  >  1:1124050/1‑150 (MQ=255)
                                                    tgtgGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTaaa                                                                                                  <  2:839485/150‑1 (MQ=255)
                                                     gtgGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTaaaa                                                                                                 >  1:1625661/1‑150 (MQ=255)
                                                       ggCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaata                                                                                               <  2:2254/150‑1 (MQ=255)
                                                        gctgctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataa                                                                                              >  2:1200268/1‑150 (MQ=255)
                                                        gctgctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataa                                                                                              >  2:1193904/1‑150 (MQ=255)
                                                         ctgcaTTCCTATTTTAACTGCAACAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataaa                                                                                             <  2:1701633/150‑1 (MQ=255)
                                                          tgctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataaat                                                                                            <  2:1373425/150‑1 (MQ=255)
                                                          tgctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataaat                                                                                            >  1:105906/1‑150 (MQ=255)
                                                           gctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaatt                                                                                           <  2:1003995/150‑1 (MQ=255)
                                                             tttCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaattaa                                                                                         <  1:1193904/150‑1 (MQ=255)
                                                             tttCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaattaa                                                                                         <  1:1200268/150‑1 (MQ=255)
                                                               tCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATATCATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaattaatt                                                                                       <  1:1732659/150‑1 (MQ=255)
                                                               tCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaattaatt                                                                                       >  1:926601/1‑150 (MQ=255)
                                                               tCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaattaatt                                                                                       <  1:1255463/150‑1 (MQ=255)
                                                                 cTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtg                                                                                     <  2:1623256/150‑1 (MQ=255)
                                                                 atATTTTAACTGCAAAAGGGAGGAGTACATTTTCAAGCGATGCAATAATATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtg                                                                                     <  2:170110/149‑1 (MQ=255)
                                                                  tATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtgc                                                                                    >  1:393909/1‑150 (MQ=255)
                                                                  tATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtgc                                                                                    >  1:413701/1‑150 (MQ=255)
                                                                  tATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtgc                                                                                    <  2:1035403/150‑1 (MQ=255)
                                                                  tATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtgc                                                                                    <  2:706135/150‑1 (MQ=255)
                                                                  tATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtga                                                                                    >  2:762157/1‑149 (MQ=255)
                                                                    ttttAACTGCAAAAGGGAGGAGTACCTTTTCATGCGATGCTATAACATTACTCATAGAAAAATACCCTTAATATCCGCTTTTTATATATTGCTGTATTGATTTAGGATATGACTCTGTGAAGGGATTATTTATTTTAATTTAATTtgctg                                                                                  >  2:1130522/1‑150 (MQ=255)
                                                                     tttAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtgctgc                                                                                 <  1:393799/150‑1 (MQ=255)
                                                                       tAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCgg                                                                               >  1:836616/1‑150 (MQ=255)
                                                                         aCTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGTGAAAATTTAAAAAAAATTAATTTGCTGCGGCt                                                                             >  2:379894/1‑150 (MQ=255)
                                                                         aCTGCAAAAGGGAGGAGTACCTTTTCAAGCGAAGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGTTATTGATTTATGAAATAACTCTTAGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCt                                                                             >  2:1091920/1‑150 (MQ=255)
                                                                          cTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCtt                                                                            >  1:850636/1‑150 (MQ=255)
                                                                            gCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCtttt                                                                          <  2:616292/150‑1 (MQ=255)
                                                                             cAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCttttt                                                                         <  2:1423484/150‑1 (MQ=255)
                                                                              aaaaGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCtttttt                                                                        <  1:1387379/150‑1 (MQ=255)
                                                                              aaaaGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCtttttt                                                                        <  2:655578/150‑1 (MQ=255)
                                                                                  gtgaggagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCATAAAAACCGCTATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATc                                                                    <  1:613005/148‑1 (MQ=255)
                                                                                  gggAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATc                                                                    >  2:505990/1‑150 (MQ=255)
                                                                                  gggAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTAAAAATAAATTAATTTGCTGCGGCTTTTTTAATc                                                                    >  1:1528451/1‑150 (MQ=255)
                                                                                   ggaggaGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATcc                                                                   >  2:1474453/1‑149 (MQ=255)
                                                                                    gaggagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGc                                                                  >  2:1683217/1‑150 (MQ=255)
                                                                                    gaggagTACCTTTTAAAGCGATGCAATAACATTACTCATATAAAAATACCCTTAAAAACCTCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAAATAATTTGCTGCGGCTTTTTTAATCGc                                                                  <  1:1112266/150‑1 (MQ=255)
                                                                                      ggagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTg                                                                <  2:506430/150‑1 (MQ=255)
                                                                                        agTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCa                                                              >  1:560713/1‑150 (MQ=255)
                                                                                         gTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCaa                                                             >  2:755574/1‑150 (MQ=255)
                                                                                            ccTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCg                                                          >  2:1531760/1‑150 (MQ=255)
                                                                                                 tCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAATAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAACATAAATTAATTTTCTGCGGCTTTTTTAATCGCTGCAACCGCAGca                                                     <  2:117267/150‑2 (MQ=255)
                                                                                                 tCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGcc                                                     <  1:1720002/150‑1 (MQ=255)
                                                                                                  cAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCt                                                    >  1:680936/1‑150 (MQ=255)
                                                                                                           cAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGc                                           >  2:889510/1‑150 (MQ=255)
                                                                                                            aaTAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGcc                                          <  1:1631152/150‑1 (MQ=255)
                                                                                                            aaTAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGcc                                          <  1:415302/150‑1 (MQ=255)
                                                                                                                   ttACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCa                                   <  1:1666137/150‑1 (MQ=255)
                                                                                                                     aCTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATc                                 <  1:895738/150‑1 (MQ=255)
                                                                                                                        cATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGAc                              >  2:1612323/1‑150 (MQ=255)
                                                                                                                            gAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTg                          <  2:106932/150‑1 (MQ=255)
                                                                                                                             aaaaaTACCCTTAAAAACAGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGc                         <  1:842677/150‑1 (MQ=255)
                                                                                                                              aaaaTACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTCACGGAAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAAACGCAGCCTTAAGCAAGCCTAAACCATCGACTTTGcc                        >  1:1230468/1‑150 (MQ=255)
                                                                                                                               aaaTACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCa                       <  1:1391104/150‑1 (MQ=255)
                                                                                                                                aaTACCATTAAAATCCGCTATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCAt                      <  2:131279/150‑1 (MQ=255)
                                                                                                                                 aTACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCAATa                     >  2:1546721/1‑150 (MQ=255)
                                                                                                                                     ccTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAagc                 <  2:1295410/150‑1 (MQ=255)
                                                                                                                                         aaaaaCCGCAATTTAAATATTGGGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGAAACCTCAGCCTTAAGCTCGCCTAACCCATCGACTTTGCCATAAAGCAGCg             >  1:262453/1‑150 (MQ=255)
                                                                                                                                         aaaaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAACCCATCGACTTTGCAATAAAGCAGCg             >  1:1260352/1‑150 (MQ=255)
                                                                                                                                         aaaaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCg             >  1:1666445/1‑150 (MQ=255)
                                                                                                                                         aaaaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCg             <  2:926601/150‑1 (MQ=255)
                                                                                                                                          aataCCGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCTCTGAAACCGCAGCCTTAAGCACTCCTAAACCATCGACTTTGCCATAATGCAGCGa            >  1:125756/4‑150 (MQ=255)
                                                                                                                                          aaaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGa            >  1:13117/1‑150 (MQ=255)
                                                                                                                                           ataCCGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAg           >  1:188899/3‑150 (MQ=255)
                                                                                                                                            aaCCGCAATTTAAAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGATTTTTTAATCGCTGCAAACGCATCCTTAACAACTACTAAACAATAGACTATGCCATAAAGCAGCGAga          >  1:46013/1‑149 (MQ=255)
                                                                                                                                             aCCGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTc         >  1:469727/1‑150 (MQ=255)
                                                                                                                                             aCCGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTc         >  2:680125/1‑150 (MQ=255)
                                                                                                                                              ccGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCa        >  1:740613/1‑150 (MQ=255)
                                                                                                                                              ccGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCa        <  2:1309185/150‑1 (MQ=255)
                                                                                                                                                 cAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAAtt     <  1:1531760/150‑1 (MQ=255)
                                                                                                                                                    tttAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAATTACt  <  1:1309538/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAATTACT  >  NC_000913/1821092‑1821389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: