Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,821,243 A→T 5.8% intergenic (‑29/+56) chbC ← / ← chbB N,N'‑diacetylchitobiose‑specific enzyme IIC component of PTS/N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,821,2430AT 5.8% 323.5 / 7.0 117intergenic (‑29/+56)chbC/chbBN,N'‑diacetylchitobiose‑specific enzyme IIC component of PTS/N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS
Reads supporting (aligned to +/- strand):  ref base A (49/61);  new base T (4/3);  total (53/64)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.22e-01

Only 100 of 118 total aligned reads displayed.

GGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAATTACTTCA  >  NC_000913/1821097‑1821392
                                                                                                                                                  |                                                                                                                                                     
gggTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAat                                                                                                                                                    <  2:16988/150‑1 (MQ=255)
gggTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAat                                                                                                                                                    <  1:1141419/150‑1 (MQ=255)
 ggTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAata                                                                                                                                                   >  1:1423484/1‑150 (MQ=255)
  gTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAatat                                                                                                                                                  >  1:559433/1‑150 (MQ=255)
   ttAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGTAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATAtt                                                                                                                                                 <  1:201078/150‑1 (MQ=255)
     aaCGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGc                                                                                                                                               >  2:286602/1‑150 (MQ=255)
     aaCGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGc                                                                                                                                               <  2:826350/150‑1 (MQ=255)
     aaCGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGc                                                                                                                                               >  2:302860/1‑150 (MQ=255)
     aaCGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGc                                                                                                                                               <  1:718680/150‑1 (MQ=255)
        ggCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATATTTAAATTGCGGt                                                                                                                                            <  1:1459169/150‑1 (MQ=255)
         gCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTa                                                                                                                                           >  2:1391104/1‑150 (MQ=255)
          cATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTAt                                                                                                                                          >  2:880118/1‑150 (MQ=255)
           aTTAAGCGAATAAAGCCATTTTTGAATGCATTAACGTGTGGCTGCTTTCCTAATTTATCTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTAtt                                                                                                                                         <  2:875183/150‑1 (MQ=255)
             tAAGCGAATAAAGCTATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGa                                                                                                                                       <  1:1674217/150‑1 (MQ=255)
              aaGCGAATAAAGCCATTTTTGATTGCATTAACGTGTTGCTGCTTTCCTATTTTAACTGCAAAAGTGAGGAGTACCTTTTCAAGAGATGCAATAACATTACTCATAGAAAAATAACCTTAAAAACAGCAATTTAAATATTGCGGAATTGAt                                                                                                                                      >  1:344334/1‑150 (MQ=255)
               aGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATATTTAAATTGCGGtttttaag                                                                                                                                     <  2:1042707/150‑5 (MQ=255)
                gCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGAttt                                                                                                                                    >  1:106932/1‑150 (MQ=255)
                 cGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTa                                                                                                                                   <  2:1660107/150‑1 (MQ=255)
                 cGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATAACCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTa                                                                                                                                   <  2:71871/150‑1 (MQ=255)
                 cGAATAAAGCAATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTa                                                                                                                                   <  1:152732/150‑1 (MQ=255)
                  gAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTAt                                                                                                                                  <  2:1241668/150‑1 (MQ=255)
                  gAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATATTTAAATTGCGGtttttaagggt                                                                                                                                  <  1:1447507/150‑8 (MQ=255)
                            aTTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAActc                                                                                                                        <  2:794708/150‑1 (MQ=255)
                             tttttGATTGCATAAACGTGTGGCTGCTTTCCTTTTTTAACTGCAATAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAActct                                                                                                                       <  2:738691/150‑1 (MQ=255)
                             tttttGATTGCAATAACGTTTGTCTGCTTTCATATATTAACTGCAAAAGGGAGTAGTACATTTTCAATCTATTCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTAAAATAATGCGGTATTGATTTATGAAATAActct                                                                                                                       <  1:278882/150‑1 (MQ=255)
                                 tGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGa                                                                                                                   >  2:576783/1‑150 (MQ=255)
                                          tAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTAACGGAAAAAt                                                                                                          >  1:920575/1‑150 (MQ=255)
                                            aCGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAAttt                                                                                                        >  2:1310202/1‑150 (MQ=255)
                                             cGTGTGGCTGCTTTCCTATTTTAACTGCAAAATGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTa                                                                                                       <  1:1069105/150‑1 (MQ=255)
                                               tgtgGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGTTGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTaaa                                                                                                     <  1:1343047/150‑1 (MQ=255)
                                               tgtgGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTaaa                                                                                                     >  1:1124050/1‑150 (MQ=255)
                                               tgtgGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTaaa                                                                                                     <  2:839485/150‑1 (MQ=255)
                                                gtgGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTaaaa                                                                                                    >  1:1625661/1‑150 (MQ=255)
                                                   gctgctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataa                                                                                                 >  2:1193904/1‑150 (MQ=255)
                                                    ctgcaTTCCTATTTTAACTGCAACAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataaa                                                                                                <  2:1701633/150‑1 (MQ=255)
                                                     tgctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataaat                                                                                               >  1:105906/1‑150 (MQ=255)
                                                      gctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaatt                                                                                              <  2:1003995/150‑1 (MQ=255)
                                                        tttCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaattaa                                                                                            <  1:1193904/150‑1 (MQ=255)
                                                        tttCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaattaa                                                                                            <  1:1200268/150‑1 (MQ=255)
                                                          tCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATATCATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaattaatt                                                                                          <  1:1732659/150‑1 (MQ=255)
                                                          tCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaattaatt                                                                                          <  1:1255463/150‑1 (MQ=255)
                                                          tCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaattaatt                                                                                          >  1:926601/1‑150 (MQ=255)
                                                            atATTTTAACTGCAAAAGGGAGGAGTACATTTTCAAGCGATGCAATAATATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtg                                                                                        <  2:170110/149‑1 (MQ=255)
                                                             tATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtgc                                                                                       <  2:1035403/150‑1 (MQ=255)
                                                             tATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtgc                                                                                       >  1:413701/1‑150 (MQ=255)
                                                             tATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtgc                                                                                       >  1:393909/1‑150 (MQ=255)
                                                             tATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtgc                                                                                       <  2:706135/150‑1 (MQ=255)
                                                             tATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtga                                                                                       >  2:762157/1‑149 (MQ=255)
                                                               ttttAACTGCAAAAGGGAGGAGTACCTTTTCATGCGATGCTATAACATTACTCATAGAAAAATACCCTTAATATCCGCTTTTTATATATTGCTGTATTGATTTAGGATATGACTCTGTGAAGGGATTATTTATTTTAATTTAATTtgctg                                                                                     >  2:1130522/1‑150 (MQ=255)
                                                                tttAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtgctgc                                                                                    <  1:393799/150‑1 (MQ=255)
                                                                  tAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCgg                                                                                  >  1:836616/1‑150 (MQ=255)
                                                                    aCTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGTGAAAATTTAAAAAAAATTAATTTGCTGCGGCt                                                                                >  2:379894/1‑150 (MQ=255)
                                                                    aCTGCAAAAGGGAGGAGTACCTTTTCAAGCGAAGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGTTATTGATTTATGAAATAACTCTTAGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCt                                                                                >  2:1091920/1‑150 (MQ=255)
                                                                       gCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCtttt                                                                             <  2:616292/150‑1 (MQ=255)
                                                                        cAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCttttt                                                                            <  2:1423484/150‑1 (MQ=255)
                                                                         aaaaGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCtttttt                                                                           <  1:1387379/150‑1 (MQ=255)
                                                                         aaaaGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCtttttt                                                                           <  2:655578/150‑1 (MQ=255)
                                                                             gtgaggagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCATAAAAACCGCTATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATc                                                                       <  1:613005/148‑1 (MQ=255)
                                                                             gggAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATc                                                                       >  2:505990/1‑150 (MQ=255)
                                                                             gggAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTAAAAATAAATTAATTTGCTGCGGCTTTTTTAATc                                                                       >  1:1528451/1‑150 (MQ=255)
                                                                              ggaggaGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATcc                                                                      >  2:1474453/1‑149 (MQ=255)
                                                                               gaggagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGc                                                                     >  2:1683217/1‑150 (MQ=255)
                                                                               gaggagTACCTTTTAAAGCGATGCAATAACATTACTCATATAAAAATACCCTTAAAAACCTCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAAATAATTTGCTGCGGCTTTTTTAATCGc                                                                     <  1:1112266/150‑1 (MQ=255)
                                                                                 ggagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTg                                                                   <  2:506430/150‑1 (MQ=255)
                                                                                   agTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCa                                                                 >  1:560713/1‑150 (MQ=255)
                                                                                    gTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCaa                                                                >  2:755574/1‑150 (MQ=255)
                                                                                       ccTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCg                                                             >  2:1531760/1‑150 (MQ=255)
                                                                                            tCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAATAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAACATAAATTAATTTTCTGCGGCTTTTTTAATCGCTGCAACCGCAGca                                                        <  2:117267/150‑2 (MQ=255)
                                                                                            tCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGcc                                                        <  1:1720002/150‑1 (MQ=255)
                                                                                                     gCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACg                                               <  1:880118/150‑1 (MQ=255)
                                                                                                      cAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGc                                              >  2:889510/1‑150 (MQ=255)
                                                                                                       aaTAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGcc                                             <  1:1631152/150‑1 (MQ=255)
                                                                                                       aaTAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGcc                                             <  1:415302/150‑1 (MQ=255)
                                                                                                              ttACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCa                                      <  1:1666137/150‑1 (MQ=255)
                                                                                                                aCTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATc                                    <  1:895738/150‑1 (MQ=255)
                                                                                                                   cATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGAc                                 >  2:1612323/1‑150 (MQ=255)
                                                                                                                       gAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTg                             <  2:106932/150‑1 (MQ=255)
                                                                                                                        aaaaaTACCCTTAAAAACAGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGc                            <  1:842677/150‑1 (MQ=255)
                                                                                                                         aaaaTACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTCACGGAAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAAACGCAGCCTTAAGCAAGCCTAAACCATCGACTTTGcc                           >  1:1230468/1‑150 (MQ=255)
                                                                                                                          aaaTACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCa                          <  1:1391104/150‑1 (MQ=255)
                                                                                                                           aaTACCATTAAAATCCGCTATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCAt                         <  2:131279/150‑1 (MQ=255)
                                                                                                                            aTACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCAATa                        >  2:1546721/1‑150 (MQ=255)
                                                                                                                              aCCATTAAAAACCTCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACTGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATaaa                      <  1:71122/150‑1 (MQ=255)
                                                                                                                                ccTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAagc                    <  2:1295410/150‑1 (MQ=255)
                                                                                                                                    aaaaaCCGCAATTTAAATATTGGGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGAAACCTCAGCCTTAAGCTCGCCTAACCCATCGACTTTGCCATAAAGCAGCg                >  1:262453/1‑150 (MQ=255)
                                                                                                                                    aaaaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAACCCATCGACTTTGCAATAAAGCAGCg                >  1:1260352/1‑150 (MQ=255)
                                                                                                                                    aaaaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCg                >  1:1666445/1‑150 (MQ=255)
                                                                                                                                    aaaaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCg                <  2:926601/150‑1 (MQ=255)
                                                                                                                                     aataCCGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCTCTGAAACCGCAGCCTTAAGCACTCCTAAACCATCGACTTTGCCATAATGCAGCGa               >  1:125756/4‑150 (MQ=255)
                                                                                                                                     aaaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGa               >  1:13117/1‑150 (MQ=255)
                                                                                                                                      ataCCGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAg              >  1:188899/3‑150 (MQ=255)
                                                                                                                                       aaCCGCAATTTAAAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGATTTTTTAATCGCTGCAAACGCATCCTTAACAACTACTAAACAATAGACTATGCCATAAAGCAGCGAga             >  1:46013/1‑149 (MQ=255)
                                                                                                                                        aCCGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTc            >  2:680125/1‑150 (MQ=255)
                                                                                                                                        aCCGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTc            >  1:469727/1‑150 (MQ=255)
                                                                                                                                         ccGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCa           >  1:740613/1‑150 (MQ=255)
                                                                                                                                         ccGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCa           <  2:1309185/150‑1 (MQ=255)
                                                                                                                                            cAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAAtt        <  1:1531760/150‑1 (MQ=255)
                                                                                                                                               tttAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAATTACt     <  1:1309538/150‑1 (MQ=255)
                                                                                                                                                 tAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAATTACTTc   <  2:96803/150‑1 (MQ=255)
                                                                                                                                                  aaaTATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAATTACTTCa  <  1:576783/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAATTACTTCA  >  NC_000913/1821097‑1821392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: