Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,823,335 G→C 9.1% intergenic (+50/‑180) nadE → / → cho NAD synthetase, NH3/glutamine‑dependent/endonuclease of nucleotide excision repair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,823,3350GC 9.1% 314.3 / 18.5 106intergenic (+50/‑180)nadE/choNAD synthetase, NH3/glutamine‑dependent/endonuclease of nucleotide excision repair
Reads supporting (aligned to +/- strand):  ref base G (51/45);  new base C (4/5);  total (56/50)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.38e-01

Only 100 of 107 total aligned reads displayed.

CTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTT  >  NC_000913/1823188‑1823480
                                                                                                                                                   |                                                                                                                                                 
cTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTTTTCACGCCGGAt                                                                                                                                                 >  2:1585407/1‑150 (MQ=255)
  cAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATgc                                                                                                                                               >  2:236938/1‑150 (MQ=255)
      aGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATgcggcg                                                                                                                                           <  1:404657/150‑1 (MQ=255)
       ggTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGATTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGt                                                                                                                                          >  2:1273543/1‑150 (MQ=255)
        gTCGCCAGAACAATAGAGCACTGGTATCTGAAAACCGAACATAAACGCCGGCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTg                                                                                                                                         <  1:828553/150‑1 (MQ=255)
        gTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGTg                                                                                                                                         <  1:562645/150‑1 (MQ=255)
        gTCGCCAGAACAATAGAGAACTCGTATCTGAAAACCGAACATAAACGCCGTCCCCCAATTACCGTTATCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTCGATTGTGCGAAAAAGACTAATTTGTTCACGCCGCATCCGGCGTg                                                                                                                                         <  1:1164958/150‑1 (MQ=255)
         tCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGTGa                                                                                                                                        <  2:1227871/150‑1 (MQ=255)
          cGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGaa                                                                                                                                       >  2:341968/1‑150 (MQ=255)
          cGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGaa                                                                                                                                       >  1:1434527/1‑150 (MQ=255)
           gCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTATTTATTTGCACATATTGGATTGGGGGAAAAAGAGTTATTTGTTCACGCCGGATGCGGCGTGAAc                                                                                                                                      >  2:282046/1‑150 (MQ=255)
           gCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAAc                                                                                                                                      >  2:1393590/1‑150 (MQ=255)
           gCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGTGAAc                                                                                                                                      <  1:1471001/150‑1 (MQ=255)
            ccAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCTAAAAAGAGTAATTTGTTCAAGCCGGATGCGGCGTGAACg                                                                                                                                     >  1:1126914/1‑150 (MQ=255)
            ccAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACg                                                                                                                                     <  1:234244/150‑1 (MQ=255)
            ccAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACg                                                                                                                                     <  1:1671027/150‑1 (MQ=255)
            ccAGAACAATAGAGAACTGGTATCTAAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACg                                                                                                                                     >  1:139870/1‑150 (MQ=255)
              aGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTGTGGAAAAAGTAAAAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGcc                                                                                                                                   >  2:836666/1‑150 (MQ=255)
              aGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGcc                                                                                                                                   <  1:1506168/150‑1 (MQ=255)
              aGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGcc                                                                                                                                   <  2:51779/150‑1 (MQ=255)
                 aCAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                <  1:1613488/150‑1 (MQ=255)
                  cAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGCATCCGGCGTGAAcaaattac                                                                                                                               <  2:1371457/150‑8 (MQ=255)
                  cAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGAGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                               >  1:1647994/1‑150 (MQ=255)
                    aTAGTGAACTGGTATCTGAAAAACGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCTGGATGCGGCGTGAACGCCTTATTc                                                                                                                             <  2:878751/150‑1 (MQ=255)
                    aTAGAGAACTGGTATCTGAAACCCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCTGGATGCGGCGTGAACGCCTTATTc                                                                                                                             <  2:884731/150‑1 (MQ=255)
                     aatagaACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCg                                                                                                                            <  1:1081590/147‑1 (MQ=255)
                      agagAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAATAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGa                                                                                                                           <  1:680731/150‑1 (MQ=255)
                       tagaACTGGTATCTGAAAAACGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGAc                                                                                                                          <  1:436091/149‑1 (MQ=255)
                        agaACTGGTATCTGAACACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAAATTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGAcc                                                                                                                         <  2:1511294/150‑1 (MQ=255)
                        agaACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGAcc                                                                                                                         <  1:1355869/150‑1 (MQ=255)
                           aattgTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCtat                                                                                                                      <  1:346176/146‑1 (MQ=255)
                              ggTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATaaa                                                                                                                   <  1:1145821/150‑1 (MQ=255)
                                tATCTGAAAACCGAACATATACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAAc                                                                                                                 <  2:13089/150‑1 (MQ=255)
                                   cTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAATAGTAATTTGTTCACTCCGGATGCGGCGTGAACCCCTTATTCGAACTATAAAAatat                                                                                                              >  2:893454/1‑150 (MQ=255)
                                   cTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTAt                                                                                                              >  2:1035642/1‑150 (MQ=255)
                                    tGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGATAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATg                                                                                                             >  2:407674/1‑150 (MQ=255)
                                    tGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATg                                                                                                             >  1:1402985/1‑150 (MQ=255)
                                     gAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGATAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGc                                                                                                            >  2:338958/1‑150 (MQ=255)
                                           cGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTc                                                                                                      <  1:528100/150‑1 (MQ=255)
                                           cGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTc                                                                                                      <  2:632242/150‑1 (MQ=255)
                                            gAACATAAACGCCGTCCGCCAATTACCGTTTTCGAAGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACTTATAAAACTATGCAAATTCa                                                                                                     <  2:1050902/150‑1 (MQ=255)
                                            gAACATAAACGCCGTCCGCCAATTACCGTGTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTAGAAAACTATGCAAATTCa                                                                                                     >  2:275822/1‑150 (MQ=255)
                                               cATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGAGCTGTAAAACTATGCAAATTCAATa                                                                                                  >  2:303628/1‑150 (MQ=255)
                                               cATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATa                                                                                                  >  1:876787/1‑150 (MQ=255)
                                                  aaaCGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACAt                                                                                               >  2:1578480/1‑150 (MQ=255)
                                                  aaaCGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACAt                                                                                               >  2:500673/1‑150 (MQ=255)
                                                  aaaCGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACAt                                                                                               >  2:585719/1‑150 (MQ=255)
                                                   aaCGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACAtt                                                                                              >  1:718887/1‑150 (MQ=255)
                                                     cGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGc                                                                                            <  2:189850/150‑1 (MQ=255)
                                                       ccGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAg                                                                                          >  1:1171092/1‑150 (MQ=255)
                                                           ccGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGt                                                                                      <  2:145812/150‑1 (MQ=255)
                                                             gCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCg                                                                                    <  1:750097/150‑1 (MQ=255)
                                                                aaTTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAAt                                                                                 >  2:1670908/1‑150 (MQ=255)
                                                                    aCCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGc                                                                             <  1:341968/150‑1 (MQ=255)
                                                                            cGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAgg                                                                     <  2:718887/150‑1 (MQ=255)
                                                                              atgatTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATTCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCg                                                                   <  1:727766/150‑1 (MQ=255)
                                                                              atgatTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCg                                                                   <  1:647621/150‑1 (MQ=255)
                                                                              atgatTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCg                                                                   >  1:1604640/1‑150 (MQ=255)
                                                                               tgatTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGt                                                                  <  2:1730273/150‑1 (MQ=255)
                                                                                 atTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAg                                                                >  2:1523644/1‑150 (MQ=255)
                                                                                  tttCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGTCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGc                                                               <  1:1265375/150‑1 (MQ=255)
                                                                                  tttCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGc                                                               >  2:120121/1‑150 (MQ=255)
                                                                                  tttATGGAAATAGTAATAATTTGCACATATTGGATTGTGCGAATAATAGTAATTTGTTCTCGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGTAAATTCTATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGc                                                               <  1:276854/150‑1 (MQ=37)
                                                                                   ttCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCa                                                              >  2:366368/1‑150 (MQ=255)
                                                                                   ttCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCa                                                              >  2:279984/1‑150 (MQ=255)
                                                                                       ggAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATATGCCTGACAGGCGTAGCACgac                                                          >  2:1565734/1‑148 (MQ=255)
                                                                                       ggAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTc                                                          <  2:887838/150‑1 (MQ=255)
                                                                                         aaaaaGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAg                                                        <  1:1393590/150‑1 (MQ=255)
                                                                                             aGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGATCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACgg                                                    <  1:653598/150‑1 (MQ=255)
                                                                                              gTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCCCGTCAGACGgt                                                   >  2:1508851/1‑150 (MQ=255)
                                                                                              gTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGgt                                                   >  2:397239/1‑150 (MQ=255)
                                                                                                   aatTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAAc                                              <  1:176435/150‑1 (MQ=255)
                                                                                                        gCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTg                                         <  2:248437/150‑1 (MQ=255)
                                                                                                        gCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTg                                         <  2:957739/150‑1 (MQ=255)
                                                                                                        gCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTg                                         <  1:1539111/150‑1 (MQ=255)
                                                                                                         cacaTATTGGATTGTGCGAAAAAGATTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGt                                        <  2:1058160/150‑1 (MQ=255)
                                                                                                         cacaTATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTATCACGTCAGACGGTGTAACCTTTGt                                        >  1:163234/1‑150 (MQ=255)
                                                                                                         cacaTATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGt                                        >  1:145801/1‑150 (MQ=255)
                                                                                                         cacaTATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGt                                        >  2:1413906/1‑150 (MQ=255)
                                                                                                             tatTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATc                                    <  2:1434527/150‑1 (MQ=255)
                                                                                                              atTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCg                                   >  1:633577/1‑150 (MQ=255)
                                                                                                              atTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCg                                   >  1:1292240/1‑150 (MQ=255)
                                                                                                                       tgCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGCCCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCCGACGGTGTAACCTTTGTCATCGACCCGccgc                          >  2:874209/1‑148 (MQ=255)
                                                                                                                       tgCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCctc                          >  2:1227355/1‑150 (MQ=255)
                                                                                                                        gCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCctct                         >  1:396550/1‑150 (MQ=255)
                                                                                                                         cGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCtt                        >  1:1603996/1‑150 (MQ=255)
                                                                                                                           aaaaaGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCATATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCgttt                      >  1:1590094/1‑150 (MQ=255)
                                                                                                                              aaGAGTAATTTGTTCACGCCGAATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTa                   >  2:1314724/1‑150 (MQ=255)
                                                                                                                               agagTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTaa                  <  1:693359/150‑1 (MQ=255)
                                                                                                                                gagTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAAt                 <  2:417003/150‑1 (MQ=255)
                                                                                                                                  gTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCg               >  1:818398/1‑150 (MQ=255)
                                                                                                                                     aggcgTTCACGCCGCATCCGGCGTGAACACCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCtt            >  2:752016/5‑150 (MQ=255)
                                                                                                                                     aTTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTTACCTTTGTCATCGACCCGCGTCTTTTTTAATCGCtt            >  1:469253/1‑150 (MQ=255)
                                                                                                                                      ggcgTTCACGCCGCATCCGGCGTGAACACCTTATTAGAACTATAAAAATATGCAAATTCAATACATTGCAGGAGTCGAATAGGCATGACAGGCGTAGCACGTCAGACGGTGTAACATTTGACATCGACCCGCCTCTTTTTTAATCGCTTc           >  2:1080941/4‑150 (MQ=255)
                                                                                                                                       ttGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTcc          >  1:775377/1‑150 (MQ=255)
                                                                                                                                         gTTCACGCCGGCTGCGGCGTGAACGCCTTATTCGACCTATAAAACTAAGCAACGTCAATACATTGCAGGAGGCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCg        <  1:638713/150‑1 (MQ=255)
                                                                                                                                         gTTCACGCCGCATCCGGCGTGAACGCCTTATTCGACCTATTAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCGTCTTTTTTAATCGCTTCCCg        >  1:75748/1‑150 (MQ=255)
                                                                                                                                              cgccgcATCCGGCGTGAACGCCTTATTAGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAAAAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGt   >  2:913543/1‑150 (MQ=255)
                                                                                                                                               gCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGtt  >  1:140443/1‑150 (MQ=255)
                                                                                                                                               gCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTGTTAATCGCTTCCCGCCTggt  >  1:858319/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGGAGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTT  >  NC_000913/1823188‑1823480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: