Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,825,936 T→A 6.2% intergenic (‑311/+19) spy ← / ← astE periplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,9360TA 6.2% 331.1 / 7.6 112intergenic (‑311/+19)spy/astEperiplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base T (54/51);  new base A (3/4);  total (57/55)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 115 total aligned reads displayed.

AATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTT  >  NC_000913/1825787‑1826085
                                                                                                                                                     |                                                                                                                                                      
aaTTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAt                                                                                                                                                        >  1:835096/1‑150 (MQ=255)
  tttGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGa                                                                                                                                                      <  2:980615/150‑1 (MQ=255)
   ttGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGAt                                                                                                                                                     <  1:1362280/150‑1 (MQ=255)
    tGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGAtt                                                                                                                                                    >  2:1350620/1‑150 (MQ=255)
        ggAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTg                                                                                                                                                <  2:1451706/150‑1 (MQ=255)
           tagggttgaTAGAAAAATACCACACGATGTATTTTCATAGATTATTCACAACAAAACCGGATGAATTGTGATAAATAAATCAAATAAACAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCgg                                                                                                                                            <  1:1333817/144‑1 (MQ=255)
            agTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCggg                                                                                                                                           <  2:147465/150‑1 (MQ=255)
            agTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCggg                                                                                                                                           <  2:1453911/150‑1 (MQ=255)
            agTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCggg                                                                                                                                           <  2:1236762/150‑1 (MQ=255)
             gTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCGGGt                                                                                                                                          <  1:1401325/150‑1 (MQ=255)
              tgattaGAGAATATTACCACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTa                                                                                                                                         <  2:825507/150‑1 (MQ=255)
              tgatgaGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTa                                                                                                                                         >  1:1022223/1‑150 (MQ=255)
              tgatgaGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCGGGTa                                                                                                                                         <  1:986133/150‑1 (MQ=255)
               gatgaGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTaa                                                                                                                                        >  2:1413806/1‑150 (MQ=255)
                 tgaGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATTATTTCTGCGGGTAAGt                                                                                                                                      >  2:937728/1‑150 (MQ=255)
                   agagaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGTGTAAGTAt                                                                                                                                    >  2:1424682/1‑150 (MQ=255)
                    gagaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATATCTGCGGGTAAGTAtt                                                                                                                                   >  2:1687322/1‑150 (MQ=255)
                     agaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCTGAATGATTTCTGCGGGTAAGTAttt                                                                                                                                  <  1:182333/150‑2 (MQ=255)
                     agaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTa                                                                                                                                  <  1:188229/150‑1 (MQ=255)
                      gaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAg                                                                                                                                 <  1:615594/150‑1 (MQ=255)
                      gaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGAAGAATGATTTCTGCGGGTAAGTATTAg                                                                                                                                 >  2:1575980/1‑150 (MQ=255)
                          atTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTa                                                                                                                             >  1:421566/1‑150 (MQ=255)
                           ttACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGATTAt                                                                                                                            >  1:1265776/1‑150 (MQ=255)
                               aacaCGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAttttt                                                                                                                        >  1:1684542/1‑150 (MQ=255)
                                acacGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAAACAGGATTAATCTGTATTAATTAATAAGAAAGCAACTTAATACCCGCATAATGATATCAGCGGGTAAGTATTATCTTAtatttt                                                                                                                       >  2:1025782/1‑150 (MQ=255)
                                             gCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATcc                                                                                                          >  1:517184/1‑150 (MQ=255)
                                             gCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATcc                                                                                                          >  2:1103612/1‑150 (MQ=255)
                                             gCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGATTATTTTTTCGAGCATTAATcc                                                                                                          >  2:86556/1‑150 (MQ=255)
                                              cAGAGATTATGAAGAACTATACCGGATGATTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTATTTTATAAGAAAGCAACTTAAAACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATccc                                                                                                         >  2:110385/1‑150 (MQ=255)
                                              cAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTATGCTTGTAAGTATTAGATTATTTTTTCGAGCATTAATccc                                                                                                         >  2:617858/1‑150 (MQ=255)
                                               agagATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcg                                                                                                        <  2:78224/150‑1 (MQ=255)
                                               agagATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcg                                                                                                        <  1:325997/150‑1 (MQ=255)
                                                    ttATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATTCCGCGCGt                                                                                                   >  1:1658726/1‑150 (MQ=255)
                                                              cTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACg                                                                                         <  1:1350620/150‑1 (MQ=255)
                                                               tataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGc                                                                                        >  1:837739/1‑150 (MQ=255)
                                                                  aCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTAc                                                                                     >  1:1357130/1‑150 (MQ=255)
                                                                   ccGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTAcc                                                                                    <  2:19410/150‑1 (MQ=255)
                                                                      gATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAAc                                                                                 <  1:1128188/150‑1 (MQ=255)
                                                                       aTGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACg                                                                                >  2:722537/1‑150 (MQ=255)
                                                                        tGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACgg                                                                               <  2:865819/150‑1 (MQ=255)
                                                                            ttgTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTa                                                                           <  2:1510084/148‑1 (MQ=255)
                                                                            tGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTa                                                                           >  2:831956/1‑150 (MQ=255)
                                                                             ggTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAg                                                                          <  1:438904/150‑1 (MQ=255)
                                                                             ggTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACACTACCAACGGATTAg                                                                          >  2:694437/1‑150 (MQ=255)
                                                                               tGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAggg                                                                        <  1:287851/150‑1 (MQ=255)
                                                                               tGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAggg                                                                        <  2:835096/150‑1 (MQ=255)
                                                                                  taaataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCTCGTAATCCCAACGCTACCAACGGATTAGGGAAt                                                                     >  2:344554/1‑150 (MQ=255)
                                                                                  taaataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAAt                                                                     >  1:1219448/1‑150 (MQ=255)
                                                                                   aaataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCTAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATa                                                                    >  2:1657129/1‑150 (MQ=255)
                                                                                   aaataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATa                                                                    >  2:980580/1‑150 (MQ=255)
                                                                                    aataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGATCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATaa                                                                   <  1:706938/150‑1 (MQ=255)
                                                                                    aataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATaa                                                                   <  2:328705/150‑1 (MQ=255)
                                                                                    aataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATaa                                                                   >  2:1249216/1‑150 (MQ=255)
                                                                                    aataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATaa                                                                   <  2:46209/150‑1 (MQ=255)
                                                                                    aataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAAACCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATaa                                                                   >  1:722715/1‑150 (MQ=255)
                                                                                     ataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAATCAACTTAATACCCGCAGAATGATTTCTGCTGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAAAGGAGTAGGGAATAac                                                                  >  2:152343/1‑150 (MQ=255)
                                                                                       aaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAacac                                                                >  1:1711778/1‑150 (MQ=255)
                                                                                       aaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAacac                                                                <  2:761991/150‑1 (MQ=255)
                                                                                         aGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCACCGCTACCAACGGATTAGGGAATAACACAt                                                              <  2:699727/150‑1 (MQ=255)
                                                                                         aGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACAt                                                              <  1:971494/150‑1 (MQ=255)
                                                                                          tcAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACAt                                                              <  1:466377/149‑1 (MQ=255)
                                                                                          gCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCAATAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATa                                                             >  2:1342459/1‑150 (MQ=255)
                                                                                           cAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTACTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATAc                                                            >  2:435428/1‑150 (MQ=255)
                                                                                             aaTAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATActc                                                          >  2:790804/1‑150 (MQ=255)
                                                                                              aTAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATActct                                                         <  2:196425/150‑1 (MQ=255)
                                                                                               tAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTa                                                        >  1:879170/1‑150 (MQ=255)
                                                                                                aaCCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAAACCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAc                                                       <  2:621685/150‑1 (MQ=255)
                                                                                                 aCCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAca                                                      <  1:1616001/150‑1 (MQ=255)
                                                                                                        ttAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAATGGATTAGGGAATAACACATACTCTACATCATggg                                               <  1:837800/150‑1 (MQ=255)
                                                                                                         tAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGt                                              <  1:1424682/150‑1 (MQ=255)
                                                                                                          aaTTTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGtt                                             <  1:594122/150‑1 (MQ=255)
                                                                                                            tCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCTGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGTGAATAACACATACTCTACATCATGGGTTAc                                           >  1:1631589/1‑150 (MQ=255)
                                                                                                              tGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACgg                                         >  2:1139348/1‑150 (MQ=255)
                                                                                                                tATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACAAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTa                                       >  1:875047/1‑150 (MQ=255)
                                                                                                                  ttAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTaaa                                     <  2:1711778/150‑1 (MQ=255)
                                                                                                                    aaTTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAAc                                   <  1:1049107/150‑1 (MQ=255)
                                                                                                                    aaTTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCAAAACGCTACCAATGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAAc                                   >  1:278686/1‑150 (MQ=255)
                                                                                                                    aaTTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGAATTAATCCCGCGCGTAATCCCAACGCTACAAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAAc                                   >  1:1025734/1‑150 (MQ=255)
                                                                                                                        tataAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGATTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTc                               >  1:610687/1‑150 (MQ=255)
                                                                                                                         ataAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTcc                              <  2:462137/150‑1 (MQ=255)
                                                                                                                         ataAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTcc                              >  2:778113/1‑150 (MQ=255)
                                                                                                                           aaGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCctc                            <  1:831956/150‑1 (MQ=255)
                                                                                                                            aGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGTGTTACGGTAAAACGTTCctct                           >  2:673553/1‑150 (MQ=255)
                                                                                                                            aGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCctct                           <  1:538845/150‑1 (MQ=255)
                                                                                                                            aGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGAGTAAGTATAAGCTAATTTTTTCGAGCATTAATACCTCGCGTAATACAAACGCTAACAACGGATTAGGGAATAACACAAACTCTACATCATGGATTACGATAAAACGTTCatct                           >  2:927647/1‑150 (MQ=255)
                                                                                                                              aaaGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTcc                         >  2:174293/1‑150 (MQ=255)
                                                                                                                               aatcAACTTAATTCCCTCATAATGATTTCTGCGTGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATTGGTTACGGTAAAACGTTCCTCTCCg                        <  2:370597/147‑1 (MQ=255)
                                                                                                                                  cAACTTAATACCCGCCGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTcc                     <  2:1083115/150‑1 (MQ=255)
                                                                                                                                    aCTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTg                   <  1:527646/150‑1 (MQ=255)
                                                                                                                                     cTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTACTCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTgc                  >  1:117696/1‑150 (MQ=255)
                                                                                                                                           aCCCGCAGAAATCATTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACTGATTAGGGAATAACACATACTCTACATCATCGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGc            >  1:790528/1‑150 (MQ=255)
                                                                                                                                            cccGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCa           >  1:511627/1‑150 (MQ=255)
                                                                                                                                            cccGCAGAAATCATTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCa           >  1:1499276/1‑150 (MQ=255)
                                                                                                                                             ccGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCaa          >  2:382695/1‑150 (MQ=255)
                                                                                                                                              cGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAAt         >  2:314850/1‑150 (MQ=255)
                                                                                                                                               gCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATg        <  1:1575980/150‑1 (MQ=255)
                                                                                                                                               gCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATg        <  2:643201/150‑1 (MQ=255)
                                                                                                                                                 aGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGtt      >  1:283655/1‑150 (MQ=255)
                                                                                                                                                    aTGATTTCTGCGGTTAAGTATTAGCTTATTTTTTCGAGCATTAAGCCAGCGAGTAATCCCAACTCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCt   <  1:110385/150‑1 (MQ=255)
                                                                                                                                                     tGATTTCTGCGTGTAAGTATTAGCTTATTTTATCGAGCATTAATCCCGCGCGTAATCCCAACGCGACCAACGGATTAGGGAATAACACATACTCTACATCATGGGGTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCtt  <  1:86426/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AATTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTT  >  NC_000913/1825787‑1826085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: