Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,825,937 G→T 6.8% intergenic (‑312/+18) spy ← / ← astE periplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,9370GT 6.8% 315.2 / 8.3 113intergenic (‑312/+18)spy/astEperiplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base G (53/52);  new base T (4/4);  total (57/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

Only 100 of 113 total aligned reads displayed.

TTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTT  >  NC_000913/1825789‑1826085
                                                                                                                                                    |                                                                                                                                                     
tttGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGa                                                                                                                                                      <  2:980615/150‑1 (MQ=255)
 ttGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGAt                                                                                                                                                     <  1:1362280/150‑1 (MQ=255)
  tGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGAtt                                                                                                                                                    >  2:1350620/1‑150 (MQ=255)
      ggAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTg                                                                                                                                                <  2:1451706/150‑1 (MQ=255)
         tagggttgaTAGAAAAATACCACACGATGTATTTTCATAGATTATTCACAACAAAACCGGATGAATTGTGATAAATAAATCAAATAAACAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCgg                                                                                                                                            <  1:1333817/144‑1 (MQ=255)
          agTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCggg                                                                                                                                           <  2:147465/150‑1 (MQ=255)
          agTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCggg                                                                                                                                           <  1:1262369/150‑1 (MQ=255)
          agTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCggg                                                                                                                                           <  2:1236762/150‑1 (MQ=255)
          agTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCggg                                                                                                                                           <  1:794912/150‑1 (MQ=255)
           gTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCGGGt                                                                                                                                          <  1:1401325/150‑1 (MQ=255)
            tgattaGAGAATATTACCACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTa                                                                                                                                         <  2:825507/150‑1 (MQ=255)
            tgatgaGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTa                                                                                                                                         >  1:1022223/1‑150 (MQ=255)
            tgatgaGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCGGGTa                                                                                                                                         <  1:986133/150‑1 (MQ=255)
             gatgaGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTaa                                                                                                                                        >  2:1413806/1‑150 (MQ=255)
               tgaGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATTATTTCTGCGGGTAAGt                                                                                                                                      >  2:937728/1‑150 (MQ=255)
                 agagaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGTGTAAGTAt                                                                                                                                    >  2:1424682/1‑150 (MQ=255)
                  gagaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATATCTGCGGGTAAGTAtt                                                                                                                                   >  2:1687322/1‑150 (MQ=255)
                   agaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCTGAATGATTTCTGCGGGTAAGTAttt                                                                                                                                  <  1:182333/150‑2 (MQ=255)
                   agaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTa                                                                                                                                  <  1:188229/150‑1 (MQ=255)
                    gaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAg                                                                                                                                 <  1:615594/150‑1 (MQ=255)
                    gaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGAAGAATGATTTCTGCGGGTAAGTATTAg                                                                                                                                 >  2:1575980/1‑150 (MQ=255)
                        atTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTa                                                                                                                             >  1:421566/1‑150 (MQ=255)
                             aacaCGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAttttt                                                                                                                        >  1:1684542/1‑150 (MQ=255)
                              acacGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAAACAGGATTAATCTGTATTAATTAATAAGAAAGCAACTTAATACCCGCATAATGATATCAGCGGGTAAGTATTATCTTAtatttt                                                                                                                       >  2:1025782/1‑150 (MQ=255)
                               cacGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCTGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTc                                                                                                                      <  1:1449605/150‑1 (MQ=255)
                                 cGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGATTATTTTTTcta                                                                                                                    >  1:370597/1‑148 (MQ=255)
                                           gCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATcc                                                                                                          >  2:1103612/1‑150 (MQ=255)
                                           gCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATcc                                                                                                          >  1:517184/1‑150 (MQ=255)
                                           gCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGATTATTTTTTCGAGCATTAATcc                                                                                                          >  2:86556/1‑150 (MQ=255)
                                            cAGAGATTATGAAGAACTATACCGGATGATTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTATTTTATAAGAAAGCAACTTAAAACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATccc                                                                                                         >  2:110385/1‑150 (MQ=255)
                                            cAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTATGCTTGTAAGTATTAGATTATTTTTTCGAGCATTAATccc                                                                                                         >  2:617858/1‑150 (MQ=255)
                                             agagATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcg                                                                                                        <  1:325997/150‑1 (MQ=255)
                                             agagATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcg                                                                                                        <  2:78224/150‑1 (MQ=255)
                                                  ttATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATTCCGCGCGt                                                                                                   >  1:1658726/1‑150 (MQ=255)
                                                            cTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACg                                                                                         <  1:1350620/150‑1 (MQ=255)
                                                             tataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGc                                                                                        >  1:837739/1‑150 (MQ=255)
                                                                aCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTAc                                                                                     >  1:1357130/1‑150 (MQ=255)
                                                                    gATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAAc                                                                                 <  1:1128188/150‑1 (MQ=255)
                                                                     aTGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACg                                                                                >  2:722537/1‑150 (MQ=255)
                                                                      tGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACgg                                                                               <  2:865819/150‑1 (MQ=255)
                                                                          ttgTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTa                                                                           <  2:1510084/148‑1 (MQ=255)
                                                                          tGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTa                                                                           >  2:831956/1‑150 (MQ=255)
                                                                           ggTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAg                                                                          <  1:438904/150‑1 (MQ=255)
                                                                           ggTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACACTACCAACGGATTAg                                                                          >  2:694437/1‑150 (MQ=255)
                                                                             tGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAggg                                                                        <  1:287851/150‑1 (MQ=255)
                                                                                taaataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCTCGTAATCCCAACGCTACCAACGGATTAGGGAAt                                                                     >  2:344554/1‑150 (MQ=255)
                                                                                taaataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAAt                                                                     >  1:1219448/1‑150 (MQ=255)
                                                                                 aaataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCTAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATa                                                                    >  2:1657129/1‑150 (MQ=255)
                                                                                 aaataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATa                                                                    >  2:980580/1‑150 (MQ=255)
                                                                                  aataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGATCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATaa                                                                   <  1:706938/150‑1 (MQ=255)
                                                                                  aataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATaa                                                                   <  2:328705/150‑1 (MQ=255)
                                                                                  aataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATaa                                                                   >  2:1249216/1‑150 (MQ=255)
                                                                                  aataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATaa                                                                   <  2:46209/150‑1 (MQ=255)
                                                                                  aataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATaa                                                                   >  1:1710100/1‑150 (MQ=255)
                                                                                   ataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAATCAACTTAATACCCGCAGAATGATTTCTGCTGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAAAGGAGTAGGGAATAac                                                                  >  2:152343/1‑150 (MQ=255)
                                                                                   ataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAAACCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAac                                                                  <  2:722715/150‑1 (MQ=255)
                                                                                     aaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAacac                                                                >  1:1711778/1‑150 (MQ=255)
                                                                                     aaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAacac                                                                <  2:761991/150‑1 (MQ=255)
                                                                                       aGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACAt                                                              <  1:971494/150‑1 (MQ=255)
                                                                                        tcAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACAt                                                              <  1:466377/149‑1 (MQ=255)
                                                                                        gCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCAATAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATa                                                             >  2:1342459/1‑150 (MQ=255)
                                                                                           aaTAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATActc                                                          >  2:790804/1‑150 (MQ=255)
                                                                                            aTAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATActct                                                         <  2:196425/150‑1 (MQ=255)
                                                                                             tAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTa                                                        >  1:879170/1‑150 (MQ=255)
                                                                                              aaCCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAAACCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAc                                                       <  2:621685/150‑1 (MQ=255)
                                                                                               aCCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAca                                                      >  1:592035/1‑150 (MQ=255)
                                                                                               aCCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAca                                                      <  1:1616001/150‑1 (MQ=255)
                                                                                                ccAGGTTTAATCTGTATTAATTTATAAGAAAGCAAATTAATAACCGCAGAATGATTTATGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATACCGCGCGGAATCCCAACGCTACCAACGGATTAGGGAATAACCCATACTCAAcaa                                                     >  2:538797/1‑149 (MQ=255)
                                                                                                      ttAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAATGGATTAGGGAATAACACATACTCTACATCATggg                                               <  1:837800/150‑1 (MQ=255)
                                                                                                       tAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGt                                              <  1:1424682/150‑1 (MQ=255)
                                                                                                        taTCTGTATTAATTTTATCCAAATCAACATAATACCCGCCGAATGCATTATGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTCATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTTCATCATGGGtt                                             <  2:329715/149‑1 (MQ=255)
                                                                                                        aaTTTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGtt                                             <  1:594122/150‑1 (MQ=255)
                                                                                                          tCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCTGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGTGAATAACACATACTCTACATCATGGGTTAc                                           >  1:1631589/1‑150 (MQ=255)
                                                                                                            tGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACgg                                         >  2:1139348/1‑150 (MQ=255)
                                                                                                              tATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACAAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTa                                       >  1:875047/1‑150 (MQ=255)
                                                                                                                ttAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTaaa                                     <  2:1711778/150‑1 (MQ=255)
                                                                                                                  aaTTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAAc                                   <  1:1049107/150‑1 (MQ=255)
                                                                                                                  aaTTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGAATTAATCCCGCGCGTAATCCCAACGCTACAAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAAc                                   >  1:1025734/1‑150 (MQ=255)
                                                                                                                      tataAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGATTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTc                               >  1:610687/1‑150 (MQ=255)
                                                                                                                       ataAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTcc                              <  2:462137/150‑1 (MQ=255)
                                                                                                                       ataAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTcc                              <  2:837739/150‑1 (MQ=255)
                                                                                                                       ataAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTcc                              >  2:778113/1‑150 (MQ=255)
                                                                                                                         aaGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCctc                            <  1:831956/150‑1 (MQ=255)
                                                                                                                          aGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGTGTTACGGTAAAACGTTCctct                           >  2:673553/1‑150 (MQ=255)
                                                                                                                          aGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCctct                           <  1:538845/150‑1 (MQ=255)
                                                                                                                          aGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGAGTAAGTATAAGCTAATTTTTTCGAGCATTAATACCTCGCGTAATACAAACGCTAACAACGGATTAGGGAATAACACAAACTCTACATCATGGATTACGATAAAACGTTCatct                           >  2:927647/1‑150 (MQ=255)
                                                                                                                            aaaGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTcc                         >  2:174293/1‑150 (MQ=255)
                                                                                                                             aatcAACTTAATTCCCTCATAATGATTTCTGCGTGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATTGGTTACGGTAAAACGTTCCTCTCCg                        <  2:370597/147‑1 (MQ=255)
                                                                                                                                cAACTTAATACCCGCCGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTcc                     <  2:1083115/150‑1 (MQ=255)
                                                                                                                                  aCTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTg                   <  1:527646/150‑1 (MQ=255)
                                                                                                                                   cTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTACTCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTgc                  >  1:117696/1‑150 (MQ=255)
                                                                                                                                     acttACCCGCAGAAATCATTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTgcgc                >  2:1444967/4‑150 (MQ=255)
                                                                                                                                         aCCCGCAGAAATCATTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACTGATTAGGGAATAACACATACTCTACATCATCGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGc            >  1:790528/1‑150 (MQ=255)
                                                                                                                                          cccGCAGAAATCATTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCa           >  1:1499276/1‑150 (MQ=255)
                                                                                                                                           ccGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCaa          >  2:382695/1‑150 (MQ=255)
                                                                                                                                            cGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAAt         >  2:314850/1‑150 (MQ=255)
                                                                                                                                             gCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATg        <  2:643201/150‑1 (MQ=255)
                                                                                                                                               aGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGtt      >  1:283655/1‑150 (MQ=255)
                                                                                                                                                  aTGATTTCTGCGGTTAAGTATTAGCTTATTTTTTCGAGCATTAAGCCAGCGAGTAATCCCAACTCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCt   <  1:110385/150‑1 (MQ=255)
                                                                                                                                                   tGATTTCTGCGTGTAAGTATTAGCTTATTTTATCGAGCATTAATCCCGCGCGTAATCCCAACGCGACCAACGGATTAGGGAATAACACATACTCTACATCATGGGGTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCtt  <  1:86426/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTTGATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTT  >  NC_000913/1825789‑1826085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: