Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,830,061 G→T 6.9% A235A (GCC→GCA astA ← arginine succinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,830,0610GT 6.9% 238.8 / 5.2 88A235A (GCC→GCAastAarginine succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (41/39);  new base T (3/4);  total (45/43)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAA  >  NC_000913/1829912‑1830203
                                                                                                                                                     |                                                                                                                                              
cccTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCgg                                                                                                                                                >  1:1435910/1‑150 (MQ=255)
 ccTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCggg                                                                                                                                               <  2:1683134/150‑1 (MQ=255)
  cTGCGCAGGCTGCCCTTCTGCCACTTCCCCCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGGCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGc                                                                                                                                              <  2:1481114/150‑1 (MQ=255)
  cTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGc                                                                                                                                              >  1:367126/1‑150 (MQ=255)
           cTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGt                                                                                                                                     <  1:803099/150‑1 (MQ=255)
            tGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAACCGTCGGCCCACCGTCAAACATGTCGAAATAGTTACGGTAGCCAAAACCTTCTTACACCAGCACCGAGCGCGCACGCGCGGtt                                                                                                                                    >  2:611606/1‑150 (MQ=255)
             gCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCCTGCCCGCGCGGtgc                                                                                                                                   <  1:1081485/150‑3 (MQ=255)
             gCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCCTGCCCGCGCGGtgc                                                                                                                                   <  1:930094/150‑3 (MQ=255)
             gCCCTTCTGCCACTTCCACCAGCCGACTTATACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGttt                                                                                                                                   <  2:481501/150‑1 (MQ=255)
                cTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCCTGCCCGCGCGGtgctgg                                                                                                                                <  1:183548/150‑6 (MQ=255)
                cTTCTGCCACTTCCACCAGCCGACTTTTACGGAAGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCCTGCCCGCGCGGtgctgg                                                                                                                                <  1:898454/150‑6 (MQ=255)
                cTTCTGCCACATCCACCAGCCGACTTTTACGGAGGGCGCGCACGCGGTCGATGTCACACGCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCg                                                                                                                                <  1:1001715/150‑1 (MQ=255)
                 ttCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCgg                                                                                                                               <  1:168623/150‑1 (MQ=255)
                 ttCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCgg                                                                                                                               <  1:208951/150‑1 (MQ=255)
                   cTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACAGTCAAAGATGTCGATATTGTTTCGGTAGCGAAACCCTTCTTTCTCAAGCAACGCTCGGGCATGCGGGGGTTGCGGAt                                                                                                                             >  2:103509/1‑150 (MQ=255)
                    tGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATg                                                                                                                            <  2:21783/150‑1 (MQ=255)
                    tGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATg                                                                                                                            <  1:303354/150‑1 (MQ=255)
                     gCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCCAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTACAGCACCGCGCGGGCAGGCGCGGTTTGCgctcaa                                                                                                                           >  1:1542439/1‑145 (MQ=255)
                     gCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCggg                                                                                                                                               <  1:1536235/130‑1 (MQ=255)
                     gCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCggg                                                                                                                                               >  2:1536235/1‑130 (MQ=255)
                     gCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGt                                                                                                                           >  2:1647079/1‑150 (MQ=255)
                     gCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGt                                                                                                                           >  1:1448599/1‑150 (MQ=255)
                       cACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTAc                                                                                                                         >  1:973032/1‑150 (MQ=255)
                       cACTACCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGGAGGCGCGGTGTGCGGATGTAc                                                                                                                         >  1:716009/1‑150 (MQ=255)
                        aCTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACACAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTAcc                                                                                                                        <  2:953264/150‑1 (MQ=255)
                           tCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAAGCGCGGTTTGCGGATGTACCTGa                                                                                                                     >  1:1458180/1‑150 (MQ=255)
                            ccaccaGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGAc                                                                                                                    >  2:1718863/1‑150 (MQ=255)
                             caccaGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAATACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGAcc                                                                                                                   >  2:861956/1‑150 (MQ=255)
                             caccaGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGAcc                                                                                                                   >  1:998327/1‑150 (MQ=255)
                                 aGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATg                                                                                                               <  2:379164/150‑1 (MQ=255)
                                   ccGACTTTTACTGATGGCGCGCACGCGGTCGATGTCACACTCAACCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGAc                                                                                                             <  2:81166/150‑1 (MQ=255)
                                      aCTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTc                                                                                                          >  1:271849/1‑150 (MQ=255)
                                          ttACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTgg                                                                                                      <  2:1361608/150‑1 (MQ=255)
                                               gATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTc                                                                                                 <  2:1435910/150‑1 (MQ=255)
                                               gATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTc                                                                                                 >  1:934105/1‑150 (MQ=255)
                                                  ggCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTg                                                                                              <  1:1536671/150‑1 (MQ=255)
                                                  ggCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTg                                                                                              <  2:1579472/150‑1 (MQ=255)
                                                   gcgcgcACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTgg                                                                                             <  1:193205/150‑1 (MQ=255)
                                                      cgcACGCGGGCGATGTCACACTCACGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGAt                                                                                          <  2:677092/150‑1 (MQ=255)
                                                        cACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGCCCGATGACGTCCTGGGCTTCCTGGGATaa                                                                                        >  2:1071552/1‑150 (MQ=255)
                                                          cgcgGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATaaaa                                                                                      >  2:1365235/1‑150 (MQ=255)
                                                                cGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTggg                                                                                <  2:1000269/150‑1 (MQ=255)
                                                                cGAGGTCACACCCCCGCGTCGGCCCACCGTCACAGATGTCGATATAGTGACGGTAGCGAACACCTTCTTTCTCCAGCACCGCGCGGGCACGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAACAGTggg                                                                                <  2:1052835/150‑1 (MQ=255)
                                                                 gATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGt                                                                               <  1:579764/150‑1 (MQ=255)
                                                                 gATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGt                                                                               <  2:1242931/150‑1 (MQ=255)
                                                                  aTGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCAATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTggtat                                                                              <  2:1300527/150‑4 (MQ=255)
                                                                      cacaCTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGa                                                                          >  2:544652/1‑150 (MQ=255)
                                                                      cacaCTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGa                                                                          >  2:708889/1‑150 (MQ=255)
                                                                      cacaCTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGa                                                                          >  1:421490/1‑150 (MQ=255)
                                                                            aaaGCGTCGGCCCACCGTCAATGATGTCGATTTAGTTACGGTAGCGAAAACGTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGGTTCCTGGGATAAAAAGTTGGTATAGATCGGAt                                                                    <  1:611606/149‑1 (MQ=255)
                                                                               gCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGCCGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGtt                                                                 >  2:407965/1‑150 (MQ=255)
                                                                                cGTCGGCCCACCGGCAAAGATGGCGATATAGTTACGGTAGGGTCACCCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGttt                                                                <  1:449673/150‑1 (MQ=255)
                                                                                   cGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCgg                                                             <  2:1417761/150‑1 (MQ=255)
                                                                                    ggCCCACAGTCAAAGATGTCGATATAGTGTCGGTAGCGAAAACCTTCTTTCTCCAGCACCCCGCGGGCAGGCGCGGTTTGAGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGTTATAGATCGGATTTTTCGGc                                                            <  2:76895/150‑1 (MQ=255)
                                                                                      cccACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCCCCGCGCGGGCAGGCGCGGTTGGCGGATGTAGCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGGGGGGAGAGATCGGATGTTTCGGcct                                                          >  1:215385/1‑148 (MQ=255)
                                                                                       ccACCGTCAAAGATGTCGATATAGTTACGTTAGCGAAAACCTTCTTGATCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATc                                                         <  2:1256056/150‑1 (MQ=255)
                                                                                         aCCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGGCCTGGGCTTCCTGGGTTTAATTGTGGGTATAGATCGGATGTTTCGGCATCAg                                                       >  2:722539/1‑150 (MQ=255)
                                                                                         aCCGTCAAAGATCACGAAATATTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGCCAGGCGCGGTTTGCGGATGTCCCTTCCCGATGACGGCCTGGGCTTCCTTGGATAAAAAGTGCGTAGAGCTCGGATGATACGGCATCac                                                       >  2:728918/1‑149 (MQ=255)
                                                                                           cGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGtt                                                     >  1:1066717/1‑150 (MQ=255)
                                                                                              cAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTg                                                  <  1:1474995/150‑1 (MQ=255)
                                                                                                    tGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATaa                                            <  2:1525785/150‑1 (MQ=255)
                                                                                                     gTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATaaa                                           <  2:367126/150‑1 (MQ=255)
                                                                                                       cGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCTGTTTGCGGATGTACCTGAAAGATGACGTCATGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCTGAATCAGTTCTGCAATAAATg                                         >  1:1528454/1‑150 (MQ=255)
                                                                                                       cGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATg                                         >  1:342694/1‑150 (MQ=255)
                                                                                                       cGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATg                                         >  1:833527/1‑150 (MQ=255)
                                                                                                        gATATAGTTACGGTAGCGAAACCCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGCTCGGATGTTTCGGCATCAGTTCTGCAATAAATGc                                        >  1:887294/1‑150 (MQ=255)
                                                                                                        gATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGc                                        >  1:1403186/1‑150 (MQ=255)
                                                                                                        gATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGc                                        >  1:223457/1‑150 (MQ=255)
                                                                                                              gTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTg                                  >  2:724634/1‑150 (MQ=255)
                                                                                                                 aCGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGGCCTGGGCTTCCTGGGATAAAACGTGGGAATAGAACGGATGTTTCTGCATCAGTTCTACAATAAATGCCTTTTGccc                               <  1:102910/150‑1 (MQ=255)
                                                                                                                   ggTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCgg                             >  2:63030/1‑150 (MQ=255)
                                                                                                                      aGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGGAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGc                          <  1:79697/150‑1 (MQ=255)
                                                                                                                        cGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCg                        <  2:598942/150‑1 (MQ=255)
                                                                                                                          aaaaCCTTCTTTCTCCAGCACCGCGCGGGCATGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCGTCCTGGGATAAAAAGTGGGTCTAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCa                      <  2:868910/150‑1 (MQ=255)
                                                                                                                          aaaaCCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCCTCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCa                      >  1:859439/1‑150 (MQ=255)
                                                                                                                          aaaaCCTTCTTTCACCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGGGGGTATAGAACGGATGTTACGGCATCAGTTATGCAATAAATGCATTTTGCCCGGTCCCGCa                      >  2:951305/1‑150 (MQ=255)
                                                                                                                             aCCTTCTTTCTCCAGCAGCGCGCGGGCAGGCGCGGTATGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAACAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCagag                   <  1:288953/150‑1 (MQ=255)
                                                                                                                             aCCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCagag                   >  2:57636/1‑150 (MQ=255)
                                                                                                                              ccTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGCTCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGACCGGTGCCGCAgaga                  >  1:678043/1‑150 (MQ=255)
                                                                                                                              ccTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAgaga                  >  2:1231584/1‑150 (MQ=255)
                                                                                                                                ttctttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGaaa                <  1:133826/150‑1 (MQ=255)
                                                                                                                                      ctcCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgc          <  1:743599/150‑1 (MQ=255)
                                                                                                                                      ctcCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATAGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGAAGAGAAAATCGgc          >  2:304310/1‑150 (MQ=255)
                                                                                                                                      ctcCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGGCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGAGCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgc          <  1:798825/150‑1 (MQ=255)
                                                                                                                                        cgcaaaCCGCGCCTGCCCGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcgc        >  2:79661/6‑150 (MQ=255)
                                                                                                                                        ccAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcgc        >  2:351051/1‑150 (MQ=255)
                                                                                                                                        ccAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcgc        >  1:536941/1‑150 (MQ=255)
                                                                                                                                         gcaaaCCGCGCCTGCCCGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGcgcg       >  1:1657536/5‑150 (MQ=255)
                                                                                                                                           aaaCCGCGCCTGCCCGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGACATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCTCAGAGAAAATCGGCGCGGc     >  2:575327/3‑150 (MQ=255)
                                                                                                                                            cACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCt    <  2:342694/150‑1 (MQ=255)
                                                                                                                                              ccGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTaa  >  1:30847/1‑150 (MQ=255)
                                                                                                                                              ccGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGAGGCCGTCCTGGGCTTCCTGGGATAAAAAGTGGGTAGAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGATAATCGGCGCGCCTaa  >  1:1623054/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
CCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAA  >  NC_000913/1829912‑1830203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: