Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,854,023 T→G 5.7% intergenic (‑44/+73) ydjE ← / ← ydjF putative MFS sugar transporter, membrane protein/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,854,0230TG 5.7% 316.6 / 7.3 105intergenic (‑44/+73)ydjE/ydjFputative MFS sugar transporter, membrane protein/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (55/44);  new base G (3/3);  total (58/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

Only 100 of 108 total aligned reads displayed.

CCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATG  >  NC_000913/1853875‑1854171
                                                                                                                                                    |                                                                                                                                                    
cccTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTATGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAAtta                                                                                                                                                     >  1:379845/1‑149 (MQ=255)
cccTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTc                                                                                                                                                     >  2:661342/1‑150 (MQ=255)
  cTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCAc                                                                                                                                                   >  1:1492069/1‑150 (MQ=255)
      taataaCAGACTAACGCTTATAATACCAAATATACGATAATGAAACCGCGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTAGGATTTGGTATAAAGGTAGCATTTGCGGCAAATTCACTTGc                                                                                                                                               >  2:444741/1‑150 (MQ=255)
      taataaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGc                                                                                                                                               >  1:817639/1‑150 (MQ=255)
       aataaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGcc                                                                                                                                              >  1:710858/1‑150 (MQ=255)
       aataaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGcc                                                                                                                                              >  2:1320612/1‑150 (MQ=255)
       aataaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGcc                                                                                                                                              <  2:560212/150‑1 (MQ=255)
          aaCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCa                                                                                                                                           <  2:801355/150‑1 (MQ=255)
           aCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCAt                                                                                                                                          >  2:901876/1‑150 (MQ=255)
           aCAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCAt                                                                                                                                          >  1:726009/1‑150 (MQ=255)
             aGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAGTGAATTTGCCGCaatt                                                                                                                                        <  1:661445/150‑4 (MQ=255)
             aGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCt                                                                                                                                        <  1:1426461/150‑1 (MQ=255)
                cTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTa                                                                                                                                     <  2:399996/150‑1 (MQ=255)
                atAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTa                                                                                                                                     <  2:705873/149‑1 (MQ=255)
                 aaaaGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTat                                                                                                                                    <  1:772440/149‑1 (MQ=255)
                  aaaGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTata                                                                                                                                   >  2:617291/1‑149 (MQ=255)
                      cTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTAtcatca                                                                                                                               <  2:217933/150‑1 (MQ=255)
                      cTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTAtcatca                                                                                                                               >  2:1000194/1‑150 (MQ=255)
                      cTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTAtcatca                                                                                                                               <  1:1232957/150‑1 (MQ=255)
                        tataATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATAATGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCAtt                                                                                                                             <  1:1517596/150‑1 (MQ=255)
                          taataCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCAtttt                                                                                                                           <  1:1313161/150‑1 (MQ=255)
                           aataCCAAATATACGATAATGAAAACGGGCCAAAGGCATTCGGTCCAGTCTTGCGCCAATTTGATCTTATTGTTCCATTAGGTGCGTCGGATTTGCTAAAAAGGTAGCAATTGCTGCAAATTCACTTGCGGCATCTTTATAATCAttatt                                                                                                                          >  1:1570520/1‑147 (MQ=255)
                             taCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTAtt                                                                                                                        <  1:810248/150‑1 (MQ=255)
                              aCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTAttt                                                                                                                       <  2:667031/150‑1 (MQ=255)
                              aCCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTAttt                                                                                                                       >  2:1119094/1‑150 (MQ=255)
                                   atttaCGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAgg                                                                                                                  <  1:959588/147‑1 (MQ=255)
                                      taCGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGgcgc                                                                                                               >  2:583970/1‑150 (MQ=255)
                                      taCGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCAATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGgcgc                                                                                                               >  1:372194/1‑150 (MQ=255)
                                        cGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAg                                                                                                             >  1:1583367/1‑150 (MQ=255)
                                          aTAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGa                                                                                                           >  2:252812/1‑150 (MQ=255)
                                           tAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGaa                                                                                                          >  1:178667/1‑150 (MQ=255)
                                            aaTGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGaaa                                                                                                         >  2:326537/1‑149 (MQ=255)
                                             aTGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACa                                                                                                        >  2:1326156/1‑150 (MQ=255)
                                             aTGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACa                                                                                                        <  1:1161030/150‑1 (MQ=255)
                                             aTGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACa                                                                                                        >  1:1094498/1‑150 (MQ=255)
                                             aTGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGACAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAACGTAGCAATTGCGGCATATTCACTTGCCGCATCTTTATCATCATTTTATTTAAAGGCGCAGGAACa                                                                                                        >  1:729408/1‑150 (MQ=255)
                                              tGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACaa                                                                                                       >  1:763096/1‑150 (MQ=255)
                                                aaaCCGGGCCAAAGGCAAGTGGGCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAaat                                                                                                     <  2:252261/150‑1 (MQ=255)
                                                  aCCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACa                                                                                                        <  2:1313864/145‑1 (MQ=255)
                                                  aCCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACa                                                                                                        >  1:1313864/1‑145 (MQ=255)
                                                  aCCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAaataa                                                                                                   >  2:855270/1‑150 (MQ=255)
                                                  aCCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAaataa                                                                                                   >  2:1020067/1‑150 (MQ=255)
                                                   ccGGGCCAAAGGCAAGCGGTACAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAaataat                                                                                                  <  1:1102742/150‑1 (MQ=255)
                                                       gCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTg                                                                                              <  2:1465437/150‑1 (MQ=255)
                                                       gCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTAAAGGCGCAGGAACAAATAATAttt                                                                                              >  2:1646619/1‑149 (MQ=255)
                                                        ccAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGa                                                                                             >  1:620115/1‑150 (MQ=255)
                                                         cAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGAc                                                                                            <  2:1444054/150‑1 (MQ=255)
                                                                    ggTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCtt                                                                                 <  2:1212597/150‑1 (MQ=255)
                                                                              tGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACcac                                                                       >  1:874873/1‑150 (MQ=255)
                                                                                cgcCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCacac                                                                     >  1:621887/1‑150 (MQ=255)
                                                                                 gcCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACAcc                                                                    >  2:788858/1‑150 (MQ=255)
                                                                                   cAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAg                                                                  <  1:544498/150‑1 (MQ=255)
                                                                                    aaTTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGc                                                                 >  1:649289/1‑150 (MQ=255)
                                                                                        tGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGa                                                             >  1:1011136/1‑150 (MQ=255)
                                                                                          aTCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGata                                                           >  2:1407162/1‑150 (MQ=255)
                                                                                           tCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGatat                                                          <  2:1492294/150‑1 (MQ=255)
                                                                                                ttGTTCCATTAGGTGCCTCTGATTTGCTAAAAATGTATCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGAGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTg                                                     <  1:125683/150‑1 (MQ=255)
                                                                                                ttGTTCCATTAGGTGCCTCGGATTTACTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTg                                                     <  1:1607629/150‑1 (MQ=255)
                                                                                                atGTTCCATTAGGTGACTCGGATTTGCTAAAAAGGTAGCAAATGCGCCAAATTCACTTGCCGAATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATattattt                                                     >  2:1193119/2‑149 (MQ=255)
                                                                                                  gTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTc                                                   <  2:399531/150‑1 (MQ=255)
                                                                                                  gTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTc                                                   <  1:1020067/150‑1 (MQ=255)
                                                                                                  gTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTc                                                   <  1:855270/150‑1 (MQ=255)
                                                                                                   ttCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCt                                                  <  2:172611/150‑1 (MQ=255)
                                                                                                     ccATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCttt                                                <  1:751885/150‑1 (MQ=255)
                                                                                                           ggTGACTAGGAATTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTAATTCAAGGCGCAGGAACAAATAATATTGACAACGTGAGCTTAAATTACAACACCAGCTGGATATAATTCTCTTTGAaaaa                                          >  2:1353764/1‑150 (MQ=255)
                                                                                                             tGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATg                                        <  1:326537/150‑1 (MQ=255)
                                                                                                              gCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGc                                       <  2:1311408/150‑1 (MQ=255)
                                                                                                                cTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCACGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCGCCCCATCGGGATATTATTGGCTTTGCAAAATGCTa                                     >  2:870808/1‑150 (MQ=255)
                                                                                                                cTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTa                                     >  2:1569182/1‑150 (MQ=255)
                                                                                                                cTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTa                                     >  2:1287823/1‑150 (MQ=255)
                                                                                                                cTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTa                                     >  2:1280874/1‑150 (MQ=255)
                                                                                                                     ttttGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCAc                                <  2:51749/149‑1 (MQ=255)
                                                                                                                        ttcTAAAAAGGTAGCAATTGCGGAAAATTCACTTTCAGAATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCActct                             <  1:195883/148‑1 (MQ=255)
                                                                                                                        tGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCActct                             <  1:74338/150‑1 (MQ=255)
                                                                                                                        tGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCActct                             <  2:807497/150‑1 (MQ=255)
                                                                                                                        tGCTAAAAAGGTAGCAATTGCGGCAAAATCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACAACACAAGCTGGATATTATTGTCTTTGCAAAATGCTATCCActct                             >  1:1416905/1‑150 (MQ=255)
                                                                                                                         gCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACAACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTg                            >  2:783082/1‑150 (MQ=255)
                                                                                                                          cTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGc                           <  2:726009/150‑1 (MQ=255)
                                                                                                                          cTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGc                           >  2:96807/1‑150 (MQ=255)
                                                                                                                          cTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATAGTCTTTGCAAAATGCTATCCACTCTGc                           >  2:87148/1‑150 (MQ=255)
                                                                                                                          cTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGATTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGc                           >  1:923073/1‑150 (MQ=255)
                                                                                                                           tAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGcc                          >  2:79979/1‑149 (MQ=255)
                                                                                                                            aaaaaGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCAc                         <  2:562647/150‑1 (MQ=255)
                                                                                                                                 ggTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCTTCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGt                    >  1:440847/1‑150 (MQ=255)
                                                                                                                                 ggTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGt                    >  1:876064/1‑150 (MQ=255)
                                                                                                                                   tAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGTCAACGTGTGCTTAAATTACCACACCAGCTGGATAATATTGTCTTTGCAAAAGGCTATCCACTCTGCACCCGGTGa                  <  1:103856/150‑1 (MQ=255)
                                                                                                                                   ataaagatGCGGCAAGTGAATTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGATATCCACTCTGCACCCGGTGa                  >  2:759891/8‑150 (MQ=255)
                                                                                                                                     gCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGAtt                >  2:304486/1‑150 (MQ=255)
                                                                                                                                       agatGCGGCAAGTGAATTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCTCACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTa              >  1:519170/4‑150 (MQ=255)
                                                                                                                                          tGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCa           <  2:1282728/150‑1 (MQ=255)
                                                                                                                                          tGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCa           <  1:1119094/150‑1 (MQ=255)
                                                                                                                                           gCGGCAAGTGAATTTGCCGCATCATTATCATCATATTATTTCAATGCGCAGTAACAAATAATATTGACACCGTGTGCTTAAATTACAACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATACACTCTGCACACGTTGATTTATCAg          >  2:195125/1‑150 (MQ=255)
                                                                                                                                           gCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTTTTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGAGTTATCAg          >  2:645323/1‑150 (MQ=255)
                                                                                                                                           gCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAg          >  2:374313/1‑150 (MQ=255)
                                                                                                                                             ggCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTCCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGtt        >  1:1630755/1‑150 (MQ=255)
                                                                                                                                               cAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGttat      <  2:391629/150‑1 (MQ=255)
                                                                                                                                                aaaTTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGttatt     <  2:1583367/150‑1 (MQ=255)
                                                                                                                                                   ttCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTTTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATg  <  1:87234/150‑1 (MQ=255)
                                                                                                                                                   ttCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATg  >  2:1015732/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCCTGTTAATAACAGACTAAAGCTTATAATACCAAATATACGATAATGAAACCGGGCCAAAGGCAAGCGGTCCAGTCTTGCGCCAATTTGATCATATTGTTCCATTAGGTGCCTCGGATTTGCTAAAAAGGTAGCAATTGCGGCAAATTCACTTGCCGCATCTTTATCATCATTTTATTTCAAGGCGCAGGAACAAATAATATTGACAACGTGTGCTTAAATTACCACACCAGCTGGATATTATTGTCTTTGCAAAATGCTATCCACTCTGCACCCGGTGATTTATCAGTTATTATG  >  NC_000913/1853875‑1854171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: