Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 216,067 A→C 5.3% intergenic (+88/+112) nlpE → / ← yaeF lipoprotein involved with copper homeostasis and adhesion/putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913216,0670AC 5.3% 379.9 / 8.7 131intergenic (+88/+112)nlpE/yaeFlipoprotein involved with copper homeostasis and adhesion/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (57/67);  new base C (3/4);  total (60/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.34e-01

Only 100 of 135 total aligned reads displayed.

TGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATAT  >  NC_000913/215918‑216216
                                                                                                                                                      |                                                                                                                                                     
tGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATaa                                                                                                                                                       >  1:599023/1‑150 (MQ=255)
 ggCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAg                                                                                                                                                      >  1:1683910/1‑150 (MQ=255)
 ggCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAg                                                                                                                                                      >  2:689340/1‑150 (MQ=255)
  gCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTACCCCGTCTTGAGACAGAAACCAACGCAAAACAGGCCAGAAGGATATATTTCATCATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAgg                                                                                                                                                     >  1:1092169/1‑150 (MQ=255)
   cACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGc                                                                                                                                                    <  1:641073/150‑1 (MQ=255)
        gATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGTGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAAGAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGtttt                                                                                                                                               <  1:1722260/150‑2 (MQ=255)
            cGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                           >  2:1530484/1‑150 (MQ=255)
             ggCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                          <  1:1682849/150‑1 (MQ=255)
              gCAGGGAAATTTTACCCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                          <  1:125602/150‑1 (MQ=255)
               cAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCa                                                                                                                                        >  2:473088/1‑150 (MQ=255)
               cAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGAACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCa                                                                                                                                        >  2:1190213/1‑150 (MQ=255)
                aGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCAt                                                                                                                                       >  1:1020942/1‑150 (MQ=255)
                 gggAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                      >  2:647898/1‑150 (MQ=255)
                 gggAAATTTTACCCCAACCAGGATTGCAGTAGATTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATGCGGCGTAAAcgccttatc                                                                                                                                      <  1:483387/150‑6 (MQ=255)
                  ggAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATAATTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                     <  1:473872/150‑1 (MQ=255)
                     aaTTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                  <  2:743861/150‑1 (MQ=255)
                      aTTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                 <  2:836634/150‑2 (MQ=255)
                         ttACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcctac                                                                                                                              <  1:1085444/150‑5 (MQ=255)
                         ttACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATg                                                                                                                              <  1:1383535/150‑1 (MQ=255)
                         ttACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATg                                                                                                                              >  1:1667174/1‑150 (MQ=255)
                           aCCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGACGCATCCGGCAATGGt                                                                                                                            >  2:186120/1‑150 (MQ=255)
                             ccccAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTg                                                                                                                           >  1:1394627/1‑150 (MQ=255)
                                cAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTc                                                                                                                        <  2:1215415/150‑1 (MQ=255)
                                    cAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACg                                                                                                                    <  1:582266/150‑1 (MQ=255)
                                      ggATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCggg                                                                                                                                  <  1:370216/134‑2 (MQ=39)
                                       gATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCt                                                                                                                 >  2:1605467/1‑150 (MQ=255)
                                        aTTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTg                                                                                                                <  2:1290842/150‑1 (MQ=255)
                                         ttGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGa                                                                                                               >  1:1355528/1‑150 (MQ=255)
                                           gCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATg                                                                                                             >  1:969153/1‑150 (MQ=255)
                                           gCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATg                                                                                                             >  2:197238/1‑150 (MQ=255)
                                           gCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATg                                                                                                             >  1:427273/1‑150 (MQ=255)
                                              gtagtTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGa                                                                                                          <  2:599023/150‑1 (MQ=255)
                                              gtagtTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGa                                                                                                          >  2:1211726/1‑150 (MQ=255)
                                                agtTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACg                                                                                                        <  1:89554/150‑1 (MQ=255)
                                                agtTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACg                                                                                                        <  1:1367243/150‑1 (MQ=255)
                                                       ggCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCCCGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGgcgc                                                                                                 >  2:1097525/1‑150 (MQ=255)
                                                       ggCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGgcgc                                                                                                 <  1:646656/150‑1 (MQ=255)
                                                          aGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTc                                                                                              <  1:206401/150‑1 (MQ=255)
                                                           gTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCt                                                                                             >  1:374104/1‑150 (MQ=255)
                                                           gTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCt                                                                                             <  2:79117/150‑1 (MQ=255)
                                                            tAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCtt                                                                                            >  2:488/1‑150 (MQ=255)
                                                             aaCCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTa                                                                                           >  1:1420246/1‑150 (MQ=255)
                                                             aaCCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTa                                                                                           <  1:689340/150‑1 (MQ=255)
                                                              aCCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                          >  2:903521/1‑150 (MQ=255)
                                                              aCCCGTATTGAGACAGAAACAAACGCAAAAAAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTATTTGTAGGCCGGATAAGGCGTTTACGCCTCATAAGTCAATGGTGCTCAACGACTCATGCGACGCTGGAGCGTCTTAt                                                                                          >  1:655443/1‑150 (MQ=38)
                                                                 cGTCTTGAGACAGAAACAACCGCAAAACAGGCCAGAAGGATATATTTCTACATTTTGATTTTGCACGTTTTTTGTAGGCCGGATTAGTCGTTTACGCCGCATCCGGCAATGGTTCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                                                       <  1:1307932/150‑1 (MQ=38)
                                                                 cGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                                                       <  1:1530484/150‑1 (MQ=255)
                                                                       gagaCAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTAc                                                                                 >  2:1232962/1‑150 (MQ=255)
                                                                         gaCAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACaa                                                                               >  2:1566444/1‑150 (MQ=255)
                                                                         gaCAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACaa                                                                               >  1:621455/1‑150 (MQ=255)
                                                                          aCAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAc                                                                              >  1:493026/1‑150 (MQ=255)
                                                                          aCAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAc                                                                              <  1:1049938/150‑1 (MQ=255)
                                                                             gAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAcccc                                                                           >  1:1120383/1‑150 (MQ=255)
                                                                             gAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAcccc                                                                           >  1:1702201/1‑150 (MQ=255)
                                                                             gAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGATTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAcccc                                                                           <  1:1151577/150‑1 (MQ=255)
                                                                               aacaaacGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAAcccccc                                                                         <  2:1683910/150‑1 (MQ=255)
                                                                                 caaacGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTc                                                                       >  1:795820/1‑150 (MQ=255)
                                                                                   aacGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCAt                                                                     <  2:1409001/150‑1 (MQ=255)
                                                                                    acGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATa                                                                    <  2:616336/150‑1 (MQ=255)
                                                                                     cGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATAc                                                                   <  1:844110/150‑1 (MQ=255)
                                                                                       cAAAACCGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCt                                                                 <  1:152138/150‑1 (MQ=255)
                                                                                         aaaCAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTa                                                               >  2:1387993/1‑150 (MQ=255)
                                                                                           agaGGCCAGTATGATATATTTAAACATTTTGAATTTGCACGTTTTTTGTTGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAg                                                             <  1:670834/148‑1 (MQ=255)
                                                                                            cAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAgg                                                            <  2:373430/150‑1 (MQ=255)
                                                                                              ggCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTTCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCg                                                          <  2:652323/150‑1 (MQ=255)
                                                                                              ggCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCg                                                          <  1:339126/150‑1 (MQ=255)
                                                                                              ggCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCg                                                          <  1:1605467/150‑1 (MQ=255)
                                                                                              ggCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCg                                                          <  1:825700/150‑1 (MQ=255)
                                                                                                ccAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGa                                                        >  2:1438870/1‑150 (MQ=255)
                                                                                                 cAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGAt                                                       >  1:361536/1‑150 (MQ=255)
                                                                                                   gAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATaa                                                     <  2:1669260/150‑1 (MQ=255)
                                                                                                          atatTTCAACTTTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACCCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCAttt                                              <  2:119703/150‑1 (MQ=255)
                                                                                                          atatTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCAttt                                              >  2:374549/1‑150 (MQ=255)
                                                                                                             tttCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACt                                           >  1:886122/1‑150 (MQ=255)
                                                                                                             tttCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGGTTACGCCGCATACGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACt                                           <  2:1661826/150‑1 (MQ=255)
                                                                                                               tCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATACCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACttt                                         >  2:287416/1‑150 (MQ=255)
                                                                                                                    aTTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCAc                                    >  2:108889/1‑150 (MQ=255)
                                                                                                                        tGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCggg                                >  1:1619446/1‑150 (MQ=255)
                                                                                                                         taaTTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGAAGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGt                               <  2:655443/149‑1 (MQ=255)
                                                                                                                           aTTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGATCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCa                             <  1:496757/150‑1 (MQ=255)
                                                                                                                               gCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCTCATCCGGCAATGGTGCTCAACGCCTGATGCGACTCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAATGCATTTACTTTATCACCGGGTCAAAcc                         <  1:1005068/150‑1 (MQ=255)
                                                                                                                               gCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAAcc                         <  2:208786/150‑1 (MQ=255)
                                                                                                                                cACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCCCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACca                        >  2:700980/1‑150 (MQ=255)
                                                                                                                                   gTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACcaaca                     <  2:299711/150‑1 (MQ=255)
                                                                                                                                      ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAg                  <  2:723606/150‑1 (MQ=255)
                                                                                                                                       tttGTAGGCCGTATCAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGc                 <  1:145609/150‑1 (MQ=255)
                                                                                                                                       tttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGc                 <  2:1394627/150‑1 (MQ=255)
                                                                                                                                       caccattgCCGGATGCGGCGTAAACGCCTTATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACCACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGc                 >  2:822739/8‑150 (MQ=38)
                                                                                                                                         ccattgCCGGATGCGGCGTAAACGCCTTATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGa               >  1:1094089/6‑150 (MQ=38)
                                                                                                                                            aGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGcccc            >  2:1298498/1‑150 (MQ=255)
                                                                                                                                             ggCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCg           >  2:545249/1‑150 (MQ=255)
                                                                                                                                              gCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGc          >  2:1586830/1‑150 (MQ=255)
                                                                                                                                               ccGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGcc         <  2:427273/150‑1 (MQ=255)
                                                                                                                                                 ggATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCtt       <  2:493026/150‑1 (MQ=255)
                                                                                                                                                  gATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTg      <  2:106528/150‑1 (MQ=255)
                                                                                                                                                    tAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGTGTCAAACCAACAAAGCGACCCGCCTTGat    <  2:1064905/150‑1 (MQ=255)
                                                                                                                                                    tAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACAGGGTCAAACCAACAAAGCGACCCGCCTTGat    >  2:1429911/1‑150 (MQ=255)
                                                                                                                                                    tAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATACCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGat    <  1:287416/150‑1 (MQ=255)
                                                                                                                                                     aaGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGata   <  1:1190213/150‑1 (MQ=255)
                                                                                                                                                      aGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGatat  <  1:1261934/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TGGCACCCGATACGGCAGGGAAATTTTACCCCAACCAGGATTGCAGTAGTTTGGGGCAGTAACCCGTCTTGAGACAGAAACAAACGCAAAACAGGCCAGAAGGATATATTTCAACATTTTGAATTTGCACGTTTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATGGTGCTCAACGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACAACCCCCCTCATACCTTAAGGCGGATAAGGCATTTACTTTATCACCGGGTCAAACCAACAAAGCGACCCGCCTTGATAT  >  NC_000913/215918‑216216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: