Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,861,425 A→C 5.3% intergenic (‑93/+277) ydjL ← / ← yeaC putative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,861,4250AC 5.3% 267.7 / 4.9 94intergenic (‑93/+277)ydjL/yeaCputative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein
Reads supporting (aligned to +/- strand):  ref base A (44/45);  new base C (3/2);  total (47/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

GACATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGG  >  NC_000913/1861276‑1861563
                                                                                                                                                     |                                                                                                                                          
gACATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAACCCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATa                                                                                                                                            >  1:432096/1‑150 (MQ=255)
 aCATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACAATAAATCTGTAAAACACAGATaa                                                                                                                                           >  1:478442/1‑150 (MQ=255)
  cATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAAt                                                                                                                                          >  1:1180454/1‑150 (MQ=255)
  cATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGGTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAAt                                                                                                                                          <  2:142778/150‑1 (MQ=255)
         catcTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGc                                                                                                                                   >  2:397387/1‑150 (MQ=255)
         catcTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGGTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGc                                                                                                                                   <  1:1130183/150‑1 (MQ=255)
           tcTTGTAGCCGCCAAAGGCCTTGCCAAACCGCGCCAGTGCTTTCATTGCGTCATCCTCTTTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGt                                                                                                                                 >  2:139951/1‑150 (MQ=255)
              tGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtttt                                                                                                                              <  2:1295734/150‑1 (MQ=255)
              tGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtttt                                                                                                                              <  1:525619/150‑1 (MQ=255)
                tAGCCGCCAAAGGCCTTGCCAAACCGCGCCAGTGAGTTCATTGAGGCATCCTCTGTGTTTTGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTAc                                                                                                                            <  2:121814/150‑1 (MQ=255)
                tAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTAc                                                                                                                            <  2:1333986/150‑1 (MQ=255)
                tAGCCACCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTCAc                                                                                                                            <  2:698578/150‑1 (MQ=255)
                 cgccgccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCAGACTTACCCCCATGATTTTTGAGGTCAACATTAAATCTGTATAACACAGATAATGATCTGCGTTTTACa                                                                                                                           >  1:163113/2‑150 (MQ=255)
                 aGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACa                                                                                                                           <  1:1400227/150‑1 (MQ=255)
                  tccgccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGAGTCATCCTCTGTGTTTAGTTTTGCTAAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACag                                                                                                                          <  2:1661092/149‑2 (MQ=255)
                  gccgccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtt                                                                                                                                <  2:166145/144‑1 (MQ=255)
                  gccgccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtt                                                                                                                                >  1:166145/1‑144 (MQ=255)
                  gccgccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGAGTCATCCGCTGTGTTTAGTGGTGATTAGCTGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACaa                                                                                                                          >  1:1143118/1‑150 (MQ=255)
                     gccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACagatt                                                                                                                       <  1:1008350/150‑5 (MQ=39)
                      ccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCa                                                                                                                      <  1:906434/150‑1 (MQ=255)
                      ccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCa                                                                                                                      <  1:498791/150‑1 (MQ=255)
                        aaaGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGa                                                                                                                    >  2:1103438/1‑150 (MQ=255)
                          aGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATc                                                                                                                  <  2:1651422/150‑1 (MQ=255)
                          aGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATc                                                                                                                  <  2:928870/150‑1 (MQ=255)
                           ggCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATca                                                                                                                 >  2:58019/1‑150 (MQ=255)
                           ggCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATca                                                                                                                 >  1:1290382/1‑150 (MQ=255)
                            gCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATcac                                                                                                                >  2:60289/1‑150 (MQ=255)
                             ccTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGAGTTGTAAAACGCAGATcatt                                                                                                               <  2:178172/150‑3 (MQ=255)
                                 gCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTa                                                                                                           >  2:804952/1‑150 (MQ=255)
                                 gCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTa                                                                                                           >  1:1283650/1‑150 (MQ=255)
                                   cAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACg                                                                                                         <  2:1622270/150‑1 (MQ=255)
                                    aaaCCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTTTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGc                                                                                                        <  1:603858/150‑1 (MQ=255)
                                    aaaCCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGc                                                                                                        >  2:1416571/1‑150 (MQ=255)
                                        cGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGATTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGaa                                                                                                    <  1:965388/150‑1 (MQ=255)
                                         gAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGTCCATCATACTTACCCACAAGATTTTTGAGGTCAACATTAAATCTGTAAACCACAGATAATGATCTGCGTTTTACAACTCAGATAAAAATTACGCAGaaa                                                                                                   >  2:290132/1‑150 (MQ=255)
                                         gAGCCAGTGCTTTCATTGCGTCATCCTCTGCGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGaaa                                                                                                   >  2:374900/1‑150 (MQ=255)
                                           gCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAAt                                                                                                 <  2:1421763/150‑1 (MQ=255)
                                             cAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATgg                                                                                               >  1:1404065/1‑150 (MQ=255)
                                             cAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATgg                                                                                               >  1:1382640/1‑150 (MQ=255)
                                               gTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCt                                                                                             >  2:1356845/1‑150 (MQ=255)
                                                ttcTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACACACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTg                                                                                            <  1:821246/148‑1 (MQ=255)
                                                    ttCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTc                                                                                        >  2:898336/1‑150 (MQ=255)
                                                    ttCATTGCGGCATCCTCTGTGTGTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTCAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggggc                                                                                        <  2:1372307/150‑3 (MQ=255)
                                                      ccttGCGGCCTCCTCTGTGGTTAGTTTTGCTTAGCTGGCCATCATACTTACCCCCATGATTTTTGCGGTCAACCTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAACTGGCGGGGTCTg                                                                                      <  2:1416667/148‑1 (MQ=255)
                                                             tCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTTCGTTTTACAACACAGATCACAATAACGAAGAAAATGGCTGGGTCTGTTACAgg                                                                               >  1:1385322/1‑150 (MQ=255)
                                                              cATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGt                                                                              >  2:1373412/1‑150 (MQ=255)
                                                              cATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGt                                                                              <  2:398477/150‑1 (MQ=255)
                                                              cATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGt                                                                              <  1:1598168/150‑1 (MQ=255)
                                                               aTCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAAATACGCAGAAAATGGCTGGGTCTGTTACAGGtt                                                                             >  1:890154/1‑150 (MQ=255)
                                                                  ttctGTGTTTATTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACTATTACGCAGAAAATGGCTGTGTCTGTTACAGGTTGAt                                                                          <  1:713922/149‑1 (MQ=255)
                                                                  ctctGTGTTTAGTTTTGATTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATATTGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                                                                          <  1:775347/150‑1 (MQ=255)
                                                                    ctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAGTTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATgg                                                                        <  1:756828/150‑1 (MQ=255)
                                                                     tgtgTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGGTTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGa                                                                       <  2:918068/150‑1 (MQ=255)
                                                                      gtgtTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGaa                                                                      <  1:592909/150‑1 (MQ=255)
                                                                           tAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGATGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACACAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCgg                                                                 <  2:7793/150‑1 (MQ=255)
                                                                               tttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGgcg                                                             <  1:898336/150‑1 (MQ=255)
                                                                               tttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGgcg                                                             >  2:1345349/1‑150 (MQ=255)
                                                                                     tAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaaa                                                       >  1:1579177/1‑150 (MQ=255)
                                                                                       gATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATATGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga                                                     >  2:732222/1‑150 (MQ=255)
                                                                                          ggCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCa                                                  >  1:921315/1‑150 (MQ=255)
                                                                                          ggCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCa                                                  <  1:1166969/150‑1 (MQ=255)
                                                                                              atcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAAtt                                              <  2:1290382/150‑1 (MQ=255)
                                                                                                  tACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGGCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCgtg                                          <  1:139951/150‑3 (MQ=255)
                                                                                                   aCTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCgaga                                         <  2:1047071/150‑1 (MQ=255)
                                                                                                       aCCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACACAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGa                                     >  2:1030253/1‑150 (MQ=255)
                                                                                                         ccACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCAGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTAAAGCTTGATGGAAGGCTGGGCGCAAAAAGAGCAAATTCGAGAcggtat                                   >  2:825754/1‑147 (MQ=255)
                                                                                                           acaTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTAc                                 >  2:1201022/1‑150 (MQ=255)
                                                                                                            caTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACACAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTAcc                                >  2:1378799/1‑150 (MQ=255)
                                                                                                             aTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCg                               >  2:15528/1‑150 (MQ=255)
                                                                                                              tGATTTTTGAGGTCAACATTAAATCTGTTAAACACAGATAATGATCTGCGTTTTACATCTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTTCAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCgg                              >  1:1318032/1‑150 (MQ=255)
                                                                                                              tGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTGGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCgg                              <  1:58019/150‑1 (MQ=255)
                                                                                                                 tttttGAGGTCAACATTAAATATGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCa                           >  2:47738/1‑150 (MQ=255)
                                                                                                                    ttgttgTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATTGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAAt                        <  1:1153099/145‑1 (MQ=255)
                                                                                                                      gAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAgg                     <  1:146485/150‑1 (MQ=255)
                                                                                                                       aGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAgg                     <  2:1283650/150‑1 (MQ=255)
                                                                                                                       aGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAgg                     >  1:182368/1‑150 (MQ=255)
                                                                                                                        ggTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGt                    >  1:428570/1‑150 (MQ=255)
                                                                                                                        ggTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGt                    >  2:185213/1‑150 (MQ=255)
                                                                                                                             aCATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTgag               <  1:541029/150‑1 (MQ=255)
                                                                                                                               aTTAAATCTTTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGAc             <  1:1577327/150‑1 (MQ=255)
                                                                                                                               aTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCTAAATAGGTCTGAGAc             >  2:1226009/1‑150 (MQ=255)
                                                                                                                               aTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGAc             >  1:628699/1‑150 (MQ=255)
                                                                                                                                  aaaaCTGGAAAACACCTAAAATGATCTGCGTGTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGACGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTa          <  1:133147/150‑1 (MQ=255)
                                                                                                                                  aaaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTa          >  2:1721400/1‑150 (MQ=255)
                                                                                                                                   aaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTaa         >  1:1367762/1‑150 (MQ=255)
                                                                                                                                   aaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTaa         >  1:577560/1‑150 (MQ=255)
                                                                                                                                       tGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTc     >  1:27477/1‑150 (MQ=255)
                                                                                                                                        gTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCa    >  1:427009/1‑150 (MQ=255)
                                                                                                                                        atAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCa    >  1:427013/2‑150 (MQ=255)
                                                                                                                                         tAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAg   >  1:390216/1‑150 (MQ=255)
                                                                                                                                          aaaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAgg  >  2:514986/1‑150 (MQ=255)
                                                                                                                                          aaaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAgg  >  2:175428/1‑150 (MQ=255)
                                                                                                                                          aaaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAgg  <  2:508194/150‑1 (MQ=255)
                                                                                                                                          aaaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAgg  <  2:1127938/150‑1 (MQ=255)
                                                                                                                                          aaaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAgg  <  1:1191818/150‑1 (MQ=255)
                                                                                                                                          aaaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAgg  <  2:1632792/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                          
GACATCAATCATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGG  >  NC_000913/1861276‑1861563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: