Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,861,434 C→T 5.4% intergenic (‑102/+268) ydjL ← / ← yeaC putative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,861,4340CT 5.4% 285.4 / 3.6 93intergenic (‑102/+268)ydjL/yeaCputative Zn‑dependent NAD(P)‑binding oxidoreductase/DUF1315 family protein
Reads supporting (aligned to +/- strand):  ref base C (43/43);  new base T (3/2);  total (47/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01

CATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAGGTGTG  >  NC_000913/1861285‑1861581
                                                                                                                                                     |                                                                                                                                                   
catcTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGc                                                                                                                                                     >  2:397387/1‑150 (MQ=255)
catcTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGGTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGc                                                                                                                                                     <  1:1130183/150‑1 (MQ=255)
  tcTTGTAGCCGCCAAAGGCCTTGCCAAACCGCGCCAGTGCTTTCATTGCGTCATCCTCTTTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGt                                                                                                                                                   >  2:139951/1‑150 (MQ=255)
     tGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtttt                                                                                                                                                <  1:525619/150‑1 (MQ=255)
     tGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtttt                                                                                                                                                <  2:1295734/150‑1 (MQ=255)
       tAGCCGCCAAAGGCCTTGCCAAACCGCGCCAGTGAGTTCATTGAGGCATCCTCTGTGTTTTGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTAc                                                                                                                                              <  2:121814/150‑1 (MQ=255)
       tAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTAc                                                                                                                                              <  2:1333986/150‑1 (MQ=255)
       tAGCCACCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTCAc                                                                                                                                              <  2:698578/150‑1 (MQ=255)
        cgccgccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCAGACTTACCCCCATGATTTTTGAGGTCAACATTAAATCTGTATAACACAGATAATGATCTGCGTTTTACa                                                                                                                                             >  1:163113/2‑150 (MQ=255)
        aGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACa                                                                                                                                             <  1:1400227/150‑1 (MQ=255)
         tccgccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGAGTCATCCTCTGTGTTTAGTTTTGCTAAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACag                                                                                                                                            <  2:1661092/149‑2 (MQ=255)
         gccgccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtt                                                                                                                                                  <  2:166145/144‑1 (MQ=255)
         gccgccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGtt                                                                                                                                                  >  1:166145/1‑144 (MQ=255)
         gccgccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGAGTCATCCGCTGTGTTTAGTGGTGATTAGCTGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACaa                                                                                                                                            >  1:1143118/1‑150 (MQ=255)
            gccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACGCAGATCATTATCTGTGTTTTACagatt                                                                                                                                         <  1:1008350/150‑5 (MQ=39)
             ccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCa                                                                                                                                        <  1:906434/150‑1 (MQ=255)
             ccAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCa                                                                                                                                        <  1:498791/150‑1 (MQ=255)
               aaaGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGa                                                                                                                                      >  2:1103438/1‑150 (MQ=255)
                 aGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATc                                                                                                                                    <  2:1651422/150‑1 (MQ=255)
                 aGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATc                                                                                                                                    <  2:928870/150‑1 (MQ=255)
                  ggCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATca                                                                                                                                   >  1:1290382/1‑150 (MQ=255)
                  ggCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATca                                                                                                                                   >  2:58019/1‑150 (MQ=255)
                   gCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATcac                                                                                                                                  >  2:60289/1‑150 (MQ=255)
                    ccTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGAGTTGTAAAACGCAGATcatt                                                                                                                                 <  2:178172/150‑3 (MQ=255)
                        gCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTa                                                                                                                             >  2:804952/1‑150 (MQ=255)
                        gCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTa                                                                                                                             >  1:1283650/1‑150 (MQ=255)
                          cAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACg                                                                                                                           <  2:1622270/150‑1 (MQ=255)
                           aaaCCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTTTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGc                                                                                                                          <  1:603858/150‑1 (MQ=255)
                           aaaCCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGc                                                                                                                          >  2:1416571/1‑150 (MQ=255)
                               cGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGATTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGaa                                                                                                                      <  1:965388/150‑1 (MQ=255)
                                gAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGTCCATCATACTTACCCACAAGATTTTTGAGGTCAACATTAAATCTGTAAACCACAGATAATGATCTGCGTTTTACAACTCAGATAAAAATTACGCAGaaa                                                                                                                     >  2:290132/1‑150 (MQ=255)
                                gAGCCAGTGCTTTCATTGCGTCATCCTCTGCGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGaaa                                                                                                                     >  2:374900/1‑150 (MQ=255)
                                  gCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAAt                                                                                                                   <  2:1421763/150‑1 (MQ=255)
                                    cAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATgg                                                                                                                 >  1:1404065/1‑150 (MQ=255)
                                    cAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATgg                                                                                                                 >  1:1382640/1‑150 (MQ=255)
                                      gTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCt                                                                                                               >  2:1356845/1‑150 (MQ=255)
                                       ttcTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACACACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTg                                                                                                              <  1:821246/148‑1 (MQ=255)
                                           ttCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTc                                                                                                          >  2:898336/1‑150 (MQ=255)
                                           ttCATTGCGGCATCCTCTGTGTGTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTCAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTggggc                                                                                                          <  2:1372307/150‑3 (MQ=255)
                                             ccttGCGGCCTCCTCTGTGGTTAGTTTTGCTTAGCTGGCCATCATACTTACCCCCATGATTTTTGCGGTCAACCTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAACTGGCGGGGTCTg                                                                                                        <  2:1416667/148‑1 (MQ=255)
                                                    tCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTTCGTTTTACAACACAGATCACAATAACGAAGAAAATGGCTGGGTCTGTTACAgg                                                                                                 >  1:1385322/1‑150 (MQ=255)
                                                     cATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGt                                                                                                <  2:398477/150‑1 (MQ=255)
                                                     cATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGt                                                                                                <  1:1598168/150‑1 (MQ=255)
                                                     cATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGt                                                                                                >  2:1373412/1‑150 (MQ=255)
                                                      aTCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAAATACGCAGAAAATGGCTGGGTCTGTTACAGGtt                                                                                               >  1:890154/1‑150 (MQ=255)
                                                         ttctGTGTTTATTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACTATTACGCAGAAAATGGCTGTGTCTGTTACAGGTTGAt                                                                                            <  1:713922/149‑1 (MQ=255)
                                                         ctctGTGTTTAGTTTTGATTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATATTGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGAt                                                                                            <  1:775347/150‑1 (MQ=255)
                                                           ctGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAGTTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATgg                                                                                          <  1:756828/150‑1 (MQ=255)
                                                            tgtgTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGGTTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGa                                                                                         <  2:918068/150‑1 (MQ=255)
                                                             gtgtTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGaa                                                                                        <  1:592909/150‑1 (MQ=255)
                                                                  tAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGATGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACACAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCgg                                                                                   <  2:7793/150‑1 (MQ=255)
                                                                      tttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGgcg                                                                               >  2:1345349/1‑150 (MQ=255)
                                                                      tttGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGgcg                                                                               <  1:898336/150‑1 (MQ=255)
                                                                            tAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCaaaaa                                                                         >  1:1579177/1‑150 (MQ=255)
                                                                              gATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATATGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAaga                                                                       >  2:732222/1‑150 (MQ=255)
                                                                                 ggCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCa                                                                    >  1:921315/1‑150 (MQ=255)
                                                                                 ggCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCa                                                                    <  1:1166969/150‑1 (MQ=255)
                                                                                     atcatACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAAtt                                                                <  2:1290382/150‑1 (MQ=255)
                                                                                         tACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGGCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCgtg                                                            <  1:139951/150‑3 (MQ=255)
                                                                                          aCTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCgaga                                                           <  2:1047071/150‑1 (MQ=255)
                                                                                              aCCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACACAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGa                                                       >  2:1030253/1‑150 (MQ=255)
                                                                                                ccACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCAGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTAAAGCTTGATGGAAGGCTGGGCGCAAAAAGAGCAAATTCGAGAcggtat                                                     >  2:825754/1‑147 (MQ=255)
                                                                                                  acaTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTAc                                                   >  2:1201022/1‑150 (MQ=255)
                                                                                                   caTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACACAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTAcc                                                  >  2:1378799/1‑150 (MQ=255)
                                                                                                    aTGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCg                                                 >  2:15528/1‑150 (MQ=255)
                                                                                                     tGATTTTTGAGGTCAACATTAAATCTGTTAAACACAGATAATGATCTGCGTTTTACATCTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTTCAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCgg                                                >  1:1318032/1‑150 (MQ=255)
                                                                                                     tGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTGGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCgg                                                <  1:58019/150‑1 (MQ=255)
                                                                                                        tttttGAGGTCAACATTAAATATGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCa                                             >  2:47738/1‑150 (MQ=255)
                                                                                                           ttgttgTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATTGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAAt                                          <  1:1153099/145‑1 (MQ=255)
                                                                                                             gAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAgg                                       <  1:146485/150‑1 (MQ=255)
                                                                                                              aGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAgg                                       >  1:182368/1‑150 (MQ=255)
                                                                                                              aGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAgg                                       <  2:1283650/150‑1 (MQ=255)
                                                                                                               ggTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGt                                      >  1:428570/1‑150 (MQ=255)
                                                                                                               ggTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGt                                      >  2:185213/1‑150 (MQ=255)
                                                                                                                    aCATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTgag                                 <  1:541029/150‑1 (MQ=255)
                                                                                                                      aTTAAATCTTTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGAc                               <  1:1577327/150‑1 (MQ=255)
                                                                                                                      aTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCTAAATAGGTCTGAGAc                               >  2:1226009/1‑150 (MQ=255)
                                                                                                                      aTTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGAc                               >  1:628699/1‑150 (MQ=255)
                                                                                                                         aaaaCTGGAAAACACCTAAAATGATCTGCGTGTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGACGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTa                            <  1:133147/150‑1 (MQ=255)
                                                                                                                         aaaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTa                            >  2:1721400/1‑150 (MQ=255)
                                                                                                                          aaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTaa                           >  1:577560/1‑150 (MQ=255)
                                                                                                                          aaTCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTaa                           >  1:1367762/1‑150 (MQ=255)
                                                                                                                              tGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTc                       >  1:27477/1‑150 (MQ=255)
                                                                                                                               gTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCa                      >  1:427009/1‑150 (MQ=255)
                                                                                                                               atAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCa                      >  1:427013/2‑150 (MQ=255)
                                                                                                                                tAAAACGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAg                     >  1:390216/1‑150 (MQ=255)
                                                                                                                                 aaaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAgg                    >  2:514986/1‑150 (MQ=255)
                                                                                                                                 aaaaCGCAGATCATTATCTGTGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAgg                    >  2:175428/1‑150 (MQ=255)
                                                                                                                                 aaaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAgg                    <  2:1127938/150‑1 (MQ=255)
                                                                                                                                 aaaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAgg                    <  1:1191818/150‑1 (MQ=255)
                                                                                                                                 aaaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAgg                    <  2:508194/150‑1 (MQ=255)
                                                                                                                                 aaaaCACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAgg                    <  2:1632792/150‑1 (MQ=255)
                                                                                                                                               gATCTGCGTTTTACAACTCAGAGCACTATTACGCAGAAAATGGCTGGGACTGTTACAGGTTGAGGGAAGGCGGGGCGCAAAAAGAGCAAAATCGAGAGGGATTCCCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAgg      <  2:289403/150‑1 (MQ=255)
                                                                                                                                               gATCTGAGTTGTAAAACGCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCACAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAgg      >  1:586025/1‑150 (MQ=255)
                                                                                                                                                   tGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGTTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAGGtgtg  >  1:439234/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CATCTTGTAGCCGCCAAAGGCCTTGCCAAACCGAGCCAGTGCTTTCATTGCGTCATCCTCTGTGTTTAGTTTTGCTTAGATGGCCATCATACTTACCCACATGATTTTTGAGGTCAACATTAAATCTGTAAAACACAGATAATGATCTGCGTTTTACAACTCAGATCACAATTACGCAGAAAATGGCTGGGTCTGTTACAGGTTGATGGAAGGCGGGGCGCAAAAAGAGCAAATTCGAGAGGGATTACCGGCCAAATAGGTCTGAGACTTAAGTTCAGGAGAAGTCATTCAGGTGTG  >  NC_000913/1861285‑1861581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: