Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,863,800 T→C 5.9% intergenic (+34/‑50) gapA → / → yeaD glyceraldehyde‑3‑phosphate dehydrogenase A/D‑hexose‑6‑phosphate epimerase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,863,8000TC 5.9% 355.1 / 7.5 117intergenic (+34/‑50)gapA/yeaDglyceraldehyde‑3‑phosphate dehydrogenase A/D‑hexose‑6‑phosphate epimerase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (62/48);  new base C (2/5);  total (64/53)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 117 total aligned reads displayed.

CTAAAGCTGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGATC  >  NC_000913/1863654‑1863946
                                                                                                                                                  |                                                                                                                                                    
cTAAAGATGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCg                                                                                                                                                   <  2:760616/150‑1 (MQ=255)
 tAAAGCTGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCgg                                                                                                                                                  >  2:839848/1‑150 (MQ=255)
 tAAAGCTGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCgg                                                                                                                                                  >  2:1610509/1‑150 (MQ=255)
 tAAAGCTGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCgg                                                                                                                                                  >  1:1678718/1‑150 (MQ=255)
  aaaGCTGGTATCGCTCTGACCGACAACTTCGTGAAACTGGTATCCTGGTACGCCAACGAAACCGGTTACTCCAACAAAGTTCTGGCCCTGATCGCTCCCATCACCAAATAAGTTGAGATGACACTGTGATCTAAAACGAGCGACTTAGGt                                                                                                                                                 >  2:174778/1‑150 (MQ=255)
  aaaGCTGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACACCAACAATGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGt                                                                                                                                                 >  2:829809/1‑150 (MQ=255)
    aGCTGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCg                                                                                                                                               <  2:896629/150‑1 (MQ=255)
      cTGGTATCGCTCTGAACGACAGCTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGct                                                                                                                                             >  1:144445/1‑150 (MQ=255)
       tGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGctc                                                                                                                                            <  2:1370619/150‑1 (MQ=255)
           aTCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCtttt                                                                                                                                      <  2:500611/150‑1 (MQ=255)
           aTCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACCGAAGTCGCTCtttt                                                                                                                                      <  1:1222746/150‑1 (MQ=255)
            tCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTTATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttt                                                                                                                                     <  1:1588957/150‑1 (MQ=255)
            tCGCGCTGAACGACACCTTCGTGAAACTGGTATCCTGGTCCGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACTCTGTGATCTAAAAAGAGCGACCGAAGTCGCTCttttt                                                                                                                                     <  2:1352517/150‑1 (MQ=255)
             cGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACCGAAGTCGCTCttttta                                                                                                                                    <  1:1649635/150‑2 (MQ=255)
              gCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttttt                                                                                                                                   >  2:825902/1‑150 (MQ=255)
              gCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttttt                                                                                                                                   <  2:1265655/150‑1 (MQ=255)
              gCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCttttttt                                                                                                                                   <  2:942427/150‑1 (MQ=255)
                  tGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTAcc                                                                                                                               <  2:1604881/150‑1 (MQ=255)
                   gAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCt                                                                                                                              <  1:910088/150‑1 (MQ=255)
                    aaCGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTg                                                                                                                             <  2:1148254/150‑1 (MQ=255)
                     aCGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGa                                                                                                                            >  1:1717262/1‑150 (MQ=255)
                     aCGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGa                                                                                                                            >  2:705444/1‑150 (MQ=255)
                      cGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGAt                                                                                                                           >  2:860445/1‑150 (MQ=255)
                         cAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATaaa                                                                                                                        >  2:402298/1‑150 (MQ=255)
                           aCTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTTGAACTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCGCTGTTTTTTACCGGAGAatat                                                                                                                      >  2:193760/1‑150 (MQ=255)
                            cTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATg                                                                                                                     <  1:1361758/150‑1 (MQ=255)
                               cGTGAAACAGGAATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATATCCAAATAAGATGAGATGACACTGTGATCTAAAAAGATCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGaaa                                                                                                                  >  1:1586518/1‑149 (MQ=255)
                                    aaCTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTaaa                                                                                                             >  2:941616/1‑150 (MQ=255)
                                      cTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAATAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAgg                                                                                                           <  1:956803/150‑1 (MQ=255)
                                      cTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAgg                                                                                                           >  1:913304/1‑150 (MQ=255)
                                      atGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAgg                                                                                                           <  1:1302823/149‑1 (MQ=255)
                                       tGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGa                                                                                                          >  2:907613/1‑150 (MQ=255)
                                        ggTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGAc                                                                                                         <  1:1404624/150‑1 (MQ=255)
                                         gTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGATCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACt                                                                                                        >  2:761450/1‑150 (MQ=255)
                                            tCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAATAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCg                                                                                                     <  1:486676/150‑1 (MQ=255)
                                             ccTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGt                                                                                                    <  1:493379/150‑1 (MQ=255)
                                               tGGTACGACAACGAACCCGGTTACTCCAACAAAGGTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTc                                                                                                   <  2:795839/150‑1 (MQ=255)
                                                          cGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATTAAATGAAGTTAAAGGACTGCGTCATGATTAAGaaa                                                                                       >  2:362992/1‑150 (MQ=255)
                                                            aaaCCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGCCATGATTAAGAAAAt                                                                                     >  2:1163474/1‑150 (MQ=255)
                                                            aaaCCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTATTTTTTTTACCTGATAAAATGAAGTAAAAGGACTGCGTCATGAATAAGAAAAt                                                                                     >  1:1095910/1‑150 (MQ=255)
                                                             aaCCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAAtt                                                                                    >  1:594015/1‑150 (MQ=255)
                                                               ccGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACTTCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAAtttt                                                                                  <  1:332788/150‑1 (MQ=255)
                                                               ccGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAAtttt                                                                                  <  2:144445/150‑1 (MQ=255)
                                                                 ggTTACTCCAACAAAGTTCTGGAACTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTg                                                                                <  1:1157255/150‑1 (MQ=255)
                                                                    tACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGccc                                                                             >  2:14281/1‑150 (MQ=255)
                                                                        ccAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGTACTGCGTCATGATTAAGAAAATTTTTGACCTTca                                                                         >  2:1166328/1‑149 (MQ=255)
                                                                        ccAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCGTTcc                                                                         >  2:1245574/1‑150 (MQ=255)
                                                                        ccAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTcc                                                                         >  1:1548193/1‑150 (MQ=255)
                                                                        ccAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTcc                                                                         >  2:1333562/1‑150 (MQ=255)
                                                                        ccAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTcc                                                                         >  2:1645126/1‑150 (MQ=255)
                                                                         caacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTTATCTAAAAAGAGCGACTTCCGTCGCTATTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGAGTCATGATTAAGAAAATTTTTGCCCTTcag                                                                        >  1:63952/1‑148 (MQ=255)
                                                                         caacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTTAAGGACTGCGTCATGATGAAGAAAATTTTTGCCCTTCCg                                                                        >  1:280314/1‑150 (MQ=255)
                                                                         caacaaAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCg                                                                        >  1:1606218/1‑150 (MQ=255)
                                                                                gTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCg                                                                 <  2:1506060/150‑1 (MQ=255)
                                                                                  tCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCTGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGAATGCGACATGATTAAGAAAATTTTTGCCCTTCCTGTCATAGaa                                                               >  1:236259/1‑150 (MQ=255)
                                                                                    tGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACa                                                             <  1:1123654/150‑1 (MQ=255)
                                                                                        ccTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAACtc                                                         >  2:61446/1‑150 (MQ=255)
                                                                                        ccTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAAtc                                                         >  2:56191/1‑150 (MQ=255)
                                                                                        ccTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGACATTCCGGTCATCTAACAAAtc                                                         >  1:165641/1‑150 (MQ=255)
                                                                                          tGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAAtctc                                                       >  2:188150/1‑150 (MQ=255)
                                                                                           gATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTGTTGCCCGAGTAATTGATGTGAAAGGACTGCGGCATGATTAAGAAAATTTTTGCGCTTCCGGTCATCGAGCataggtcc                                                      >  2:1166632/1‑143 (MQ=255)
                                                                                             tCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTcccc                                                    <  1:1079849/150‑1 (MQ=255)
                                                                                               gCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCATTCCCGTCATCGAACAAAACTCCCCTg                                                  >  2:1093501/1‑150 (MQ=255)
                                                                                                cTCACATCTCCAAATAATTTGAGATGACACTGTGATCTAAAAAGATCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGt                                                 <  1:1549126/150‑1 (MQ=255)
                                                                                                  aacaTCTCCAAATATGTTGAGATGACACTGTTATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTcc                                               <  1:1667842/149‑1 (MQ=255)
                                                                                                    caTCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCctc                                             >  2:1511341/1‑150 (MQ=255)
                                                                                                       ctcCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTTCCTGATATAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTcc                                          >  2:209270/1‑150 (MQ=255)
                                                                                                       ctcCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTcc                                          <  2:963777/150‑1 (MQ=255)
                                                                                                        tcCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGATAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTcc                                          >  2:48842/1‑150 (MQ=255)
                                                                                                        tcCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTccc                                         <  1:825902/150‑1 (MQ=255)
                                                                                                          aaaaTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAATTGAAGTTAAATGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgt                                       <  2:1586518/149‑1 (MQ=255)
                                                                                                           aaaTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTATTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgtc                                      >  2:1088473/1‑150 (MQ=255)
                                                                                                            aaTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgtcg                                     <  2:755568/150‑1 (MQ=255)
                                                                                                            aaTAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTCTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgtcg                                     <  2:1505218/150‑1 (MQ=255)
                                                                                                               ctttttagaTCACAGTGTCATCTCAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTaa                                  >  2:187886/7‑150 (MQ=255)
                                                                                                               aaGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGATCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTaa                                  <  1:1670064/150‑1 (MQ=255)
                                                                                                                 gTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAAc                                <  1:860445/150‑1 (MQ=255)
                                                                                                                  ttGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACt                               <  2:1717262/150‑1 (MQ=255)
                                                                                                                   tGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTg                              <  2:1678718/150‑1 (MQ=255)
                                                                                                                      gaTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGAt                           <  2:776935/150‑1 (MQ=255)
                                                                                                                       aTGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATg                          <  1:1416180/150‑1 (MQ=255)
                                                                                                                        tGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATg                          <  1:595112/150‑1 (MQ=255)
                                                                                                                          acacTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCATCGTAAACTGGATGAAc                       >  2:1640304/1‑150 (MQ=255)
                                                                                                                             cTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCATTCCGGTCATCAAACAAATATCCCCTGTCCTCTCCATTCGTAAACTGGATTAACTgg                    >  2:152996/1‑150 (MQ=255)
                                                                                                                                tgATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGAcc                 <  2:1722870/150‑1 (MQ=255)
                                                                                                                                  aTCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAATTTAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTTTCCTCTCCAGTCGTAAACTGGATTAAATGGAcgta               >  1:132745/1‑147 (MQ=255)
                                                                                                                                   tCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCTTAAACTGGATGAACTGGAGCtat              >  1:907480/1‑148 (MQ=255)
                                                                                                                                    cTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCAt             >  1:699894/1‑150 (MQ=255)
                                                                                                                                     tAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTAATCGAACAAATCTCCCCAGTCCTCTCCCGTCGTAAAATGGGTGAACTGGACCTCAtt            >  2:590509/1‑150 (MQ=255)
                                                                                                                                      aaaaaGAGCGACTTCGGTCGCTCTTATTTTTTACCTGAAAAAAAGAAGTTAAAGGACTGCGTCATGAATAAGAAAATTTTAGCCCTTCCGGTCATCGAACAAATATCCCCTGTCCTCACACCTCGTAAACTGGATGAACTGTACCTCAtt            >  2:735227/1‑150 (MQ=255)
                                                                                                                                      aaaaaGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATtg           >  2:160185/1‑150 (MQ=255)
                                                                                                                                      aaaaaGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGATAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCGTCCGGTCATCGAACAAAACTCCCCTGTCCTCTCCGGTCGTAAACTGGATGAACTGGACCTCATtg           >  2:86237/1‑150 (MQ=255)
                                                                                                                                      aaaaaGAGCGACCGAAGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATtg           >  2:839379/1‑150 (MQ=255)
                                                                                                                                       aaaaGAGCGCCTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATtgt          >  1:688341/1‑150 (MQ=255)
                                                                                                                                       aaaaGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTATACTGGATGAACTGGCCCTCATtgt          >  1:694600/1‑150 (MQ=255)
                                                                                                                                       aaaaGAGCGACCGAAGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATtgt          >  2:15917/1‑150 (MQ=255)
                                                                                                                                         aaGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGCCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTgg        <  1:1163474/150‑1 (MQ=255)
                                                                                                                                          agagCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGt       <  1:1333562/150‑1 (MQ=255)
                                                                                                                                             gCGACTTCGGTCGCTCTTTTTTTTACCTGAAAAAATGAAGATAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCATTCCGGTCAACGAACAAATCTCCCCTGTCCTCACCCGACGTAAACAGGATCAACTGGAACTCATTGTGGTcta    >  2:1219686/1‑148 (MQ=255)
                                                                                                                                               gACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTTCCCTTCCGGTCATCTAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGatc  >  1:1040274/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CTAAAGCTGGTATCGCTCTGAACGACAACTTCGTGAAACTGGTATCCTGGTACGACAACGAAACCGGTTACTCCAACAAAGTTCTGGACCTGATCGCTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGACCTCATTGTGGTCGATC  >  NC_000913/1863654‑1863946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: