Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,758 G→A 8.5% intergenic (+24/+24) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7580GA 8.5% 263.9 / 10.7 94intergenic (+24/+24)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (40/45);  new base A (5/3);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

GATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGC  >  NC_000913/1864609‑1864907
                                                                                                                                                     |                                                                                                                                                     
gATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCggg                                                                                                                                                       <  1:1087161/150‑1 (MQ=255)
gATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCggg                                                                                                                                                       <  2:1348165/150‑1 (MQ=255)
gATATGCCGGATGATGGCTACAAAACATTTGGTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGCGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCggg                                                                                                                                                       <  1:1068707/150‑1 (MQ=255)
  tatGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTg                                                                                                                                                     <  2:1497690/150‑1 (MQ=255)
     gCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTGCaa                                                                                                                                                  >  1:1503901/1‑150 (MQ=255)
      ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAt                                                                                                                                                 <  1:978214/150‑1 (MQ=255)
       cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                <  2:731108/150‑1 (MQ=255)
        ggATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGc                                                                                                                                               >  1:436108/1‑150 (MQ=255)
               ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGc                                                                                                                                        <  2:58564/150‑1 (MQ=255)
               ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGc                                                                                                                                        <  2:1016360/150‑1 (MQ=255)
               ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGc                                                                                                                                        <  1:898140/150‑1 (MQ=255)
                gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                       <  2:537729/150‑1 (MQ=255)
                gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                       <  2:193207/150‑1 (MQ=255)
                gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTAGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                       <  2:968507/150‑1 (MQ=255)
                 cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                      <  1:794871/150‑1 (MQ=255)
                 cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                      <  1:1241124/150‑1 (MQ=255)
                 cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                      >  2:389227/1‑150 (MQ=255)
                 cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                      <  1:1602452/150‑1 (MQ=255)
                 cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                      <  1:1321121/150‑1 (MQ=255)
                 cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                      <  1:838504/150‑1 (MQ=255)
                 cTACAAAACATTTGTGTGTGTAGAAACGGCTTACGCTTCAGAAAAGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCCTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                      <  1:1235040/150‑1 (MQ=255)
                  acacaaaCAAATGTTTTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAAAGTTAATTTACGTTAATGTTGTGTTCCGGGTGCAATGCATCCGGcacac                                                                                                                                    >  1:860555/5‑150 (MQ=255)
                   aCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcacat                                                                                                                                    <  1:397688/150‑2 (MQ=255)
                    cAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGcacacc                                                                                                                                   >  2:1303918/1‑149 (MQ=255)
                     aaaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaa                                                                                                                                  <  2:389530/150‑1 (MQ=255)
                     aaaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaa                                                                                                                                  >  2:720737/1‑150 (MQ=255)
                      aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaac                                                                                                                                 <  1:905381/150‑1 (MQ=255)
                        aCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaacct                                                                                                                               >  1:979167/1‑148 (MQ=255)
                         cATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGAAATGCATCCGGCACACAACATc                                                                                                                              >  1:187956/1‑150 (MQ=255)
                             tgtttgtGTAGAAACGGCTTACGCTACAGAAACGAAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTACGGGTGCAATTCATCCGGCACACAACATCacac                                                                                                                          >  2:1314871/1‑150 (MQ=255)
                                  gtgtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGAACATCTGGCGCAATCCATTCGCGTGGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCatat                                                                                                                     >  2:199894/1‑150 (MQ=255)
                                    gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATcc                                                                                                                   >  2:1162318/1‑150 (MQ=255)
                                    gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCCGCCCACAACATCACACCatatac                                                                                                                   >  2:684991/1‑148 (MQ=255)
                                    gtAGAAAAGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAAACTGCAAATCTGGCGCAATCCATTCGCGTTGCGCAACGTTAATTTACTTTAATTTTGTGTGCAGGGTGCAATGAATCCGGCACACAACATCACACCATATcc                                                                                                                   >  2:1043978/1‑150 (MQ=255)
                                     tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCa                                                                                                                  >  2:744401/1‑150 (MQ=255)
                                          aCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGAGCAATCCATTCGCGTTGAGAAACGTTAAATTACCTTAATGTTGTGTGCCGGGTGCAATTCATCCCGCACACAACATAACACCATATACatagca                                                                                                             >  2:566275/1‑145 (MQ=255)
                                                 aCGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTc                                                                                                      <  1:1004269/150‑1 (MQ=255)
                                                 aCGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTc                                                                                                      >  1:1052538/1‑150 (MQ=255)
                                                  ggCTTCAGAAACGCAAAAATTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTcc                                                                                                     <  2:1508596/149‑1 (MQ=255)
                                                  cGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTcc                                                                                                     <  1:1210800/150‑1 (MQ=255)
                                                    cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACCCAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                   >  1:1579312/1‑150 (MQ=255)
                                                     ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCttt                                                                                                  <  2:397393/150‑1 (MQ=255)
                                                          aaaCGAAAAATGTGACAAAATATAAACCTGCACATCTGGCGCATTCCATTCGCGTTGCTAAAGGTTAATTTACGTAAATGTTGTGTGCCGGGTGCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTg                                                                                             <  2:1342197/150‑1 (MQ=255)
                                                           aaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACCCCATATCCAGCGCAGTTTTTCCTTTTTGTGc                                                                                            >  1:382548/1‑150 (MQ=255)
                                                           aaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATAACACCATATGCAGCGCAGTTGTTCCTTTTGGTGc                                                                                            >  1:463079/1‑150 (MQ=255)
                                                               cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACCCAACGTCACACCATTTCCAGCGCAGTTTTTCCTTTTGGTGCCggt                                                                                        >  2:734166/1‑149 (MQ=255)
                                                                     gtcaCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTAACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                                                                  <  1:651197/147‑1 (MQ=255)
                                                                     gTGACCAAAGAGAAACCTGCACATCTGGCTCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTGGTGTGCCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                                                                  <  2:740300/150‑1 (MQ=255)
                                                                     gTGACCAAAGACAAACCTGAACATCTGGCGCAATCCATTCGCGTTGCGAAAGGTTATTTTACGTTAATGTTGTGTGCCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                                                                  <  2:753255/150‑1 (MQ=255)
                                                                      tGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCTAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCt                                                                                 <  2:1460481/150‑1 (MQ=255)
                                                                      tGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCt                                                                                 <  2:1052538/150‑1 (MQ=255)
                                                                       gACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCtt                                                                                >  2:1526449/1‑150 (MQ=255)
                                                                        aCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCAGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTa                                                                               <  2:1136624/150‑1 (MQ=255)
                                                                         ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTAAATTTACGTTAATGTTGTGTGACGGGTGCAATGCATACGGCACACAACATCCCACCATATCCAGCGCAGTTTTTCCTTATGGTGCCGGATATGCCTTAt                                                                              >  1:909252/1‑150 (MQ=255)
                                                                           aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATcc                                                                            >  1:1205044/1‑150 (MQ=255)
                                                                              gagaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGc                                                                         <  1:756348/150‑1 (MQ=255)
                                                                               agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCa                                                                        >  1:1038248/1‑150 (MQ=255)
                                                                                   aCCTGCACATCTGGCGCAATCAATTCGCGTTGCTAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGc                                                                    <  2:1349778/150‑1 (MQ=255)
                                                                                    ccTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACCCAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCt                                                                   >  1:157986/1‑150 (MQ=255)
                                                                                       ccacaTCTGGCGCAATCCTTTCGCGTGGCGAAAGTTTAATTTAGTTTATTTTTGTGTGCCGGTTGAAATGCATCGGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAt                                                                <  2:1503901/149‑1 (MQ=255)
                                                                                        cacaTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAtt                                                               >  1:1608134/1‑150 (MQ=255)
                                                                                        cacaTCTGGCGCAATCCATTCGCGATGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTTCAATTCATACGGCACACAAAAACACACCATATCCAGGGCAGTTTTTACTTTTTGTGCCGGATATGGCTTATACAGCATAGCTAAtt                                                               >  1:597485/1‑150 (MQ=255)
                                                                                         acaTCTGGCGCAAACCATTCGCGTGGCGAAACGTTAATTTACGTTAATGTTGGGGGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTc                                                              <  2:1721672/150‑1 (MQ=255)
                                                                                          caTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACCCAACATCCCCCCATATCCAGAGCAGTATTTCCTTTTTGTGCCGGATATGACTTATCCAGCATAGcgcattga                                                             >  2:764924/1‑143 (MQ=255)
                                                                                          caTCTGGCGAAATCCATTCGCGTTGCGAAACGTTAATTTACGTAAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTcc                                                             <  1:1400691/150‑1 (MQ=255)
                                                                                             cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACTCAACATAACACCATATACAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                          >  2:874801/1‑150 (MQ=255)
                                                                                             cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                          <  1:1583129/150‑1 (MQ=255)
                                                                                              tGGCGCAATCCATTCGCGTGGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg                                                         <  2:1712471/150‑1 (MQ=255)
                                                                                               ggCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTga                                                        >  1:1234482/1‑150 (MQ=255)
                                                                                               ggCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGACGGGTGCAATGCATCCGGCACACAACATCACACCATTTACAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTga                                                        >  1:280887/1‑150 (MQ=255)
                                                                                                gcgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACAAAACAACATATACAGCGCAGTTTTTCCTTATGGTGCCGGATATGACATATCCAGCATAGCTAATTCCGcataa                                                       >  1:735680/1‑146 (MQ=255)
                                                                                                 cgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTGCAATGAATCAGGCAAACAACATAACACCATATACAGCCCAGGTTTTACGTTTGGTGCCGGTTTTGGCTTATACAGCATAGCTAATTGCGCTgaac                                                      >  2:1424814/1‑149 (MQ=255)
                                                                                                 cgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaag                                                      <  1:723484/150‑1 (MQ=255)
                                                                                                 cgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATAACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaag                                                      >  1:666889/1‑150 (MQ=255)
                                                                                                 cgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAAAATCACACCATATACAGCGCAGTTTTTGCTTTTGGTGCCGGATATTCCATATCCAGCATATCTAATTCCGCTgaag                                                      >  1:1649819/1‑150 (MQ=255)
                                                                                                  gcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaaga                                                     >  1:1392207/1‑150 (MQ=255)
                                                                                                    aaTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGaaa                                                   >  2:1726212/1‑150 (MQ=255)
                                                                                                       ccATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGAAATGCATACGGCACACAAACTAAACCCATATACAGCGCAGGTTTTACTTTTTGTTGCCGATAATCCATATACAACAAAGATAATTAAACTGAAGaaatat                                                >  2:1326048/1‑147 (MQ=255)
                                                                                                       ccATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGGAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGtt                                                <  1:1041728/150‑1 (MQ=255)
                                                                                                        cATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCAAATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATACAGCATAGCTAATTCCGCTGAAGAAAGttg                                               >  2:570042/1‑149 (MQ=255)
                                                                                                            cgcgTTGCGAAACGTAAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGAcc                                           <  2:235792/150‑1 (MQ=255)
                                                                                                               gTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCGGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCa                                        <  1:1499393/150‑1 (MQ=255)
                                                                                                                 tGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAg                                      <  2:491952/150‑1 (MQ=255)
                                                                                                                  gctaaaCTTTATTTTACTTTATTGTTGTGTGCCGGGTGAAATGATTCGGGAACAAAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                     <  1:587382/147‑1 (MQ=255)
                                                                                                                     aaaCGTTAATTTACGTTAATGTGGTGTGCGGTGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcaca                                  <  1:1700365/150‑1 (MQ=255)
                                                                                                                            aaTTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACCCACGATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCCGCATAGCTAATGCCGCTGCAGATTGTTCGACCTCAAGGACCGCCGCag                           >  1:917754/1‑149 (MQ=255)
                                                                                                                              tttACGTTATTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAttt                         <  2:1696118/150‑1 (MQ=255)
                                                                                                                              tttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAttt                         <  2:860555/150‑1 (MQ=255)
                                                                                                                                tACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttg                       <  2:1362343/150‑1 (MQ=255)
                                                                                                                                       ttgttggGTGCCGGGTCCAAGGAATCGGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACa                <  1:1673148/149‑1 (MQ=255)
                                                                                                                                        tgttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACAt               <  2:1392207/150‑1 (MQ=255)
                                                                                                                                           tgtgggCCGGGTGAAAGGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATggg            <  1:1409969/150‑1 (MQ=255)
                                                                                                                                             tgtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCa          >  1:1633924/1‑150 (MQ=255)
                                                                                                                                              gtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCaa         >  2:947855/1‑150 (MQ=255)
                                                                                                                                               tgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAAt        >  2:485760/1‑150 (MQ=255)
                                                                                                                                                gCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGCCCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc       >  1:1399278/1‑150 (MQ=255)
                                                                                                                                                gCCGGATGCATTGCACACGGCACACAACATCACACCATATCCAGCGCAGTTGTTACTTTTGGTGCCGGATATGCCTTATACAGCATAGGTAATTACGCTCAAGAAAGTACGACCTCACGAACAGCCGCATTTTGTTTGACATTGGCAATc       >  1:349447/1‑150 (MQ=255)
                                                                                                                                                     gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTgcc  <  1:389227/150‑3 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGC  >  NC_000913/1864609‑1864907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: