Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,768 T→C 8.0% intergenic (+34/+14) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7680TC 8.0% 250.2 / 12.5 100intergenic (+34/+14)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (43/48);  new base C (5/3);  total (48/52)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.33e-02

GGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGG  >  NC_000913/1864624‑1864916
                                                                                                                                                |                                                                                                                                                    
ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGc                                                                                                                                                 <  2:1016360/150‑1 (MQ=255)
ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGc                                                                                                                                                 <  1:898140/150‑1 (MQ=255)
ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGc                                                                                                                                                 <  2:58564/150‑1 (MQ=255)
 gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                                <  2:193207/150‑1 (MQ=255)
 gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                                <  2:537729/150‑1 (MQ=255)
 gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTAGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                                <  2:968507/150‑1 (MQ=255)
  cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                               <  1:794871/150‑1 (MQ=255)
  cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                               <  1:1241124/150‑1 (MQ=255)
  cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                               >  2:389227/1‑150 (MQ=255)
  cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                               <  1:1602452/150‑1 (MQ=255)
  cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                               <  1:1321121/150‑1 (MQ=255)
  cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcac                                                                                                                                               <  1:838504/150‑1 (MQ=255)
  cTACAAAACATTTGTGTGTGTAGAAACGGCTTACGCTTCAGAAAAGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCCTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                               <  1:1235040/150‑1 (MQ=255)
   acacaaaCAAATGTTTTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAAAGTTAATTTACGTTAATGTTGTGTTCCGGGTGCAATGCATCCGGcacac                                                                                                                                             >  1:860555/5‑150 (MQ=255)
    aCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGcacat                                                                                                                                             <  1:397688/150‑2 (MQ=255)
     cAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGcacacc                                                                                                                                            >  2:1303918/1‑149 (MQ=255)
      aaaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaa                                                                                                                                           >  2:720737/1‑150 (MQ=255)
      aaaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaa                                                                                                                                           <  2:389530/150‑1 (MQ=255)
       aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaac                                                                                                                                          <  1:905381/150‑1 (MQ=255)
         aCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaacct                                                                                                                                        >  1:979167/1‑148 (MQ=255)
          cATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGAAATGCATCCGGCACACAACATc                                                                                                                                       >  1:187956/1‑150 (MQ=255)
              tgtttgtGTAGAAACGGCTTACGCTACAGAAACGAAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTACGGGTGCAATTCATCCGGCACACAACATCacac                                                                                                                                   >  2:1314871/1‑150 (MQ=255)
                   gtgtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGAACATCTGGCGCAATCCATTCGCGTGGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCatat                                                                                                                              >  2:199894/1‑150 (MQ=255)
                     gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATcc                                                                                                                            >  2:1162318/1‑150 (MQ=255)
                     gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCCGCCCACAACATCACACCatatac                                                                                                                            >  2:684991/1‑148 (MQ=255)
                     gtAGAAAAGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAAACTGCAAATCTGGCGCAATCCATTCGCGTTGCGCAACGTTAATTTACTTTAATTTTGTGTGCAGGGTGCAATGAATCCGGCACACAACATCACACCATATcc                                                                                                                            >  2:1043978/1‑150 (MQ=255)
                      tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCa                                                                                                                           >  2:744401/1‑150 (MQ=255)
                           aCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGAGCAATCCATTCGCGTTGAGAAACGTTAAATTACCTTAATGTTGTGTGCCGGGTGCAATTCATCCCGCACACAACATAACACCATATACatagca                                                                                                                      >  2:566275/1‑145 (MQ=255)
                                  aCGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTc                                                                                                               >  1:1052538/1‑150 (MQ=255)
                                  aCGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTc                                                                                                               <  1:1004269/150‑1 (MQ=255)
                                   ggCTTCAGAAACGCAAAAATTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTcc                                                                                                              <  2:1508596/149‑1 (MQ=255)
                                   cGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTcc                                                                                                              <  1:1210800/150‑1 (MQ=255)
                                     cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACCCAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                            >  1:1579312/1‑150 (MQ=255)
                                      ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCttt                                                                                                           <  2:397393/150‑1 (MQ=255)
                                           aaaCGAAAAATGTGACAAAATATAAACCTGCACATCTGGCGCATTCCATTCGCGTTGCTAAAGGTTAATTTACGTAAATGTTGTGTGCCGGGTGCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTg                                                                                                      <  2:1342197/150‑1 (MQ=255)
                                            aaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACCCCATATCCAGCGCAGTTTTTCCTTTTTGTGc                                                                                                     >  1:382548/1‑150 (MQ=255)
                                            aaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATAACACCATATGCAGCGCAGTTGTTCCTTTTGGTGc                                                                                                     >  1:463079/1‑150 (MQ=255)
                                                cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACCCAACGTCACACCATTTCCAGCGCAGTTTTTCCTTTTGGTGCCggt                                                                                                 >  2:734166/1‑149 (MQ=255)
                                                      gtcaCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTAACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                                                                           <  1:651197/147‑1 (MQ=255)
                                                      gTGACCAAAGAGAAACCTGCACATCTGGCTCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTGGTGTGCCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                                                                           <  2:740300/150‑1 (MQ=255)
                                                      gTGACCAAAGACAAACCTGAACATCTGGCGCAATCCATTCGCGTTGCGAAAGGTTATTTTACGTTAATGTTGTGTGCCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                                                                           <  2:753255/150‑1 (MQ=255)
                                                       tGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCTAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCt                                                                                          <  2:1460481/150‑1 (MQ=255)
                                                       tGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCt                                                                                          <  2:1052538/150‑1 (MQ=255)
                                                        gACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCtt                                                                                         >  2:1526449/1‑150 (MQ=255)
                                                         aCCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCAGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTa                                                                                        <  2:1136624/150‑1 (MQ=255)
                                                          ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTAAATTTACGTTAATGTTGTGTGACGGGTGCAATGCATACGGCACACAACATCCCACCATATCCAGCGCAGTTTTTCCTTATGGTGCCGGATATGCCTTAt                                                                                       >  1:909252/1‑150 (MQ=255)
                                                            aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATcc                                                                                     >  1:1205044/1‑150 (MQ=255)
                                                               gagaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGc                                                                                  <  1:756348/150‑1 (MQ=255)
                                                                agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCa                                                                                 >  1:1038248/1‑150 (MQ=255)
                                                                    aCCTGCACATCTGGCGCAATCAATTCGCGTTGCTAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGc                                                                             <  2:1349778/150‑1 (MQ=255)
                                                                     ccTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACCCAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCt                                                                            >  1:157986/1‑150 (MQ=255)
                                                                        ccacaTCTGGCGCAATCCTTTCGCGTGGCGAAAGTTTAATTTAGTTTATTTTTGTGTGCCGGTTGAAATGCATCGGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAt                                                                         <  2:1503901/149‑1 (MQ=255)
                                                                         cacaTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAtt                                                                        >  1:1608134/1‑150 (MQ=255)
                                                                         cacaTCTGGCGCAATCCATTCGCGATGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTTCAATTCATACGGCACACAAAAACACACCATATCCAGGGCAGTTTTTACTTTTTGTGCCGGATATGGCTTATACAGCATAGCTAAtt                                                                        >  1:597485/1‑150 (MQ=255)
                                                                          acaTCTGGCGCAAACCATTCGCGTGGCGAAACGTTAATTTACGTTAATGTTGGGGGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTc                                                                       <  2:1721672/150‑1 (MQ=255)
                                                                           caTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACCCAACATCCCCCCATATCCAGAGCAGTATTTCCTTTTTGTGCCGGATATGACTTATCCAGCATAGcgcattga                                                                      >  2:764924/1‑143 (MQ=255)
                                                                           caTCTGGCGAAATCCATTCGCGTTGCGAAACGTTAATTTACGTAAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTcc                                                                      <  1:1400691/150‑1 (MQ=255)
                                                                              cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACTCAACATAACACCATATACAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                                   >  2:874801/1‑150 (MQ=255)
                                                                              cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                                   <  1:1583129/150‑1 (MQ=255)
                                                                               tGGCGCAATCCATTCGCGTGGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTg                                                                  <  2:1712471/150‑1 (MQ=255)
                                                                                ggCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTga                                                                 >  1:1234482/1‑150 (MQ=255)
                                                                                ggCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGACGGGTGCAATGCATCCGGCACACAACATCACACCATTTACAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTga                                                                 >  1:280887/1‑150 (MQ=255)
                                                                                 gcgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACAAAACAACATATACAGCGCAGTTTTTCCTTATGGTGCCGGATATGACATATCCAGCATAGCTAATTCCGcataa                                                                >  1:735680/1‑146 (MQ=255)
                                                                                  cgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTTCCGGGTGCAATGAATCAGGCAAACAACATAACACCATATACAGCCCAGGTTTTACGTTTGGTGCCGGTTTTGGCTTATACAGCATAGCTAATTGCGCTgaac                                                               >  2:1424814/1‑149 (MQ=255)
                                                                                  cgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaag                                                               <  1:723484/150‑1 (MQ=255)
                                                                                  cgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATAACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaag                                                               >  1:666889/1‑150 (MQ=255)
                                                                                  cgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAAAATCACACCATATACAGCGCAGTTTTTGCTTTTGGTGCCGGATATTCCATATCCAGCATATCTAATTCCGCTgaag                                                               >  1:1649819/1‑150 (MQ=255)
                                                                                   gcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaaga                                                              >  1:1392207/1‑150 (MQ=255)
                                                                                     aaTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGaaa                                                            >  2:1726212/1‑150 (MQ=255)
                                                                                        ccATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGAAATGCATACGGCACACAAACTAAACCCATATACAGCGCAGGTTTTACTTTTTGTTGCCGATAATCCATATACAACAAAGATAATTAAACTGAAGaaatat                                                         >  2:1326048/1‑147 (MQ=255)
                                                                                        ccATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGGAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGtt                                                         <  1:1041728/150‑1 (MQ=255)
                                                                                         cATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCAAATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATACAGCATAGCTAATTCCGCTGAAGAAAGttg                                                        >  2:570042/1‑149 (MQ=255)
                                                                                             cgcgTTGCGAAACGTAAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGAcc                                                    <  2:235792/150‑1 (MQ=255)
                                                                                                gTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCGGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCa                                                 <  1:1499393/150‑1 (MQ=255)
                                                                                                  tGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAg                                               <  2:491952/150‑1 (MQ=255)
                                                                                                   gctaaaCTTTATTTTACTTTATTGTTGTGTGCCGGGTGAAATGATTCGGGAACAAAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                              <  1:587382/147‑1 (MQ=255)
                                                                                                      aaaCGTTAATTTACGTTAATGTGGTGTGCGGTGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcaca                                           <  1:1700365/150‑1 (MQ=255)
                                                                                                             aaTTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACCCACGATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCCGCATAGCTAATGCCGCTGCAGATTGTTCGACCTCAAGGACCGCCGCag                                    >  1:917754/1‑149 (MQ=255)
                                                                                                               tttACGTTATTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAttt                                  <  2:1696118/150‑1 (MQ=255)
                                                                                                               tttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAttt                                  <  2:860555/150‑1 (MQ=255)
                                                                                                                 tACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttg                                <  2:1362343/150‑1 (MQ=255)
                                                                                                                        ttgttggGTGCCGGGTCCAAGGAATCGGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACa                         <  1:1673148/149‑1 (MQ=255)
                                                                                                                         tgttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACAt                        <  2:1392207/150‑1 (MQ=255)
                                                                                                                            tgtgggCCGGGTGAAAGGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATggg                     <  1:1409969/150‑1 (MQ=255)
                                                                                                                              tgtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCa                   >  1:1633924/1‑150 (MQ=255)
                                                                                                                               gtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCaa                  >  2:947855/1‑150 (MQ=255)
                                                                                                                                tgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAAt                 >  2:485760/1‑150 (MQ=255)
                                                                                                                                 gCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGCCCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc                >  1:1399278/1‑150 (MQ=255)
                                                                                                                                 gCCGGATGCATTGCACACGGCACACAACATCACACCATATCCAGCGCAGTTGTTACTTTTGGTGCCGGATATGCCTTATACAGCATAGGTAATTACGCTCAAGAAAGTACGACCTCACGAACAGCCGCATTTTGTTTGACATTGGCAATc                >  1:349447/1‑150 (MQ=255)
                                                                                                                                      gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTgcc           <  1:389227/150‑3 (MQ=255)
                                                                                                                                        gCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCACGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCg         >  2:1533386/1‑150 (MQ=255)
                                                                                                                                        gCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCg         >  2:1725378/1‑150 (MQ=255)
                                                                                                                                         cAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCACGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGc        >  1:1699785/1‑150 (MQ=255)
                                                                                                                                          aaTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCt       <  2:979167/150‑1 (MQ=255)
                                                                                                                                          aaTGCATCCGGCAAACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGTTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCt       <  2:464824/150‑1 (MQ=255)
                                                                                                                                           aTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGcgt      >  2:1115528/1‑148 (MQ=255)
                                                                                                                                           aTGCAGCCGGCAAACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCTAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCtt      <  1:525383/150‑1 (MQ=255)
                                                                                                                                              cATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCTGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTg   <  2:932156/150‑1 (MQ=255)
                                                                                                                                              cATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTg   <  2:436108/150‑1 (MQ=255)
                                                                                                                                               aTCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCTATCGTGGCCGCTTTTgg  >  2:1482137/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGG  >  NC_000913/1864624‑1864916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: