Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,912,748 T→G 6.5% intergenic (‑172/+20) htpX ← / ← prc putative endopeptidase/carboxy‑terminal protease for penicillin‑binding protein 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,912,7480TG 6.5% 399.0 / 15.6 138intergenic (‑172/+20)htpX/prcputative endopeptidase/carboxy‑terminal protease for penicillin‑binding protein 3
Reads supporting (aligned to +/- strand):  ref base T (73/55);  new base G (7/2);  total (80/58)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.78e-01

Only 100 of 141 total aligned reads displayed.

CAAAACGCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTT  >  NC_000913/1912601‑1912896
                                                                                                                                                   |                                                                                                                                                    
cAAAACGCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAAttt                                                                                                                                                    >  2:785906/1‑150 (MQ=255)
 aaaaCGCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTc                                                                                                                                                   >  2:954260/1‑150 (MQ=255)
 aaaaCGCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTATCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAAATTTACATTTTGTTGCATCTGATTTACGGCATCTTGTCGCTGTAAAAAAATAAGGCAAAAttta                                                                                                                                                   >  1:1020758/1‑149 (MQ=255)
  aaaCGCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCt                                                                                                                                                  >  2:103/1‑150 (MQ=255)
  aaaCGCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCt                                                                                                                                                  >  2:1583641/1‑150 (MQ=255)
    aCGCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTtg                                                                                                                                                <  1:1697032/150‑1 (MQ=255)
      gCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTTAAAAATCAGGCACAATTTCTtgtg                                                                                                                                              >  1:1657204/1‑150 (MQ=255)
         atatGCGATACCCACATCGTATGTGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAAGAAATTGTGCCt                                                                                                                                           <  2:1500218/150‑1 (MQ=255)
          tatGCGATACCCAAATCGTATGTGTTACTCGACTAATTTCAAGTCTGTATAGTGCGTAAGTAAAAAGAAAGACACATCTTTACATTTTGTAGCATCTGCTTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAAATTCTTGTGCCtg                                                                                                                                          <  1:289184/150‑1 (MQ=255)
            tGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCtgat                                                                                                                                        >  2:1215598/1‑150 (MQ=255)
            tGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCtgat                                                                                                                                        >  2:935238/1‑150 (MQ=255)
             gCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCtgatt                                                                                                                                       <  2:1449665/150‑1 (MQ=255)
               gATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCtgattga                                                                                                                                     >  1:1719719/1‑150 (MQ=255)
                aTACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCtgattgat                                                                                                                                    >  2:335608/1‑150 (MQ=255)
                 tACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAATTCACCAGAATGACACAAATTTACATTTTGTAGCTTCTGATGTACGGCATCATGTCGCTGTTAAAAAATCAGGCACTCTTTCTTGAGCCTGATTGata                                                                                                                                   <  1:32643/150‑1 (MQ=255)
                   cccACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATAtt                                                                                                                                 >  1:1346898/1‑150 (MQ=255)
                    ccACATCCTATGGGTTACGCGACTATTTTCAAGTCTGGAGAGTGCGGAAGTCACCAGAAAGACACAACGTTACATTTTGTAGCATCTGAATTACGGCATCTTGTCGCTGTAAAAAAAGCCGGCACAATTTCTTGTGCCTGATTGATATTa                                                                                                                                <  1:897066/150‑1 (MQ=255)
                     cacaTCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTaa                                                                                                                               >  1:1388396/1‑149 (MQ=255)
                           gTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACg                                                                                                                         >  2:703277/1‑150 (MQ=255)
                           gTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACg                                                                                                                         <  2:909306/150‑1 (MQ=255)
                            aaTGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAATTCACCAGAAATACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACgg                                                                                                                        <  1:1622576/149‑1 (MQ=255)
                                   tACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCggg                                                                                                                 <  1:1303592/150‑1 (MQ=255)
                                      gcgACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTATACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGgttg                                                                                                              <  2:86491/150‑1 (MQ=255)
                                       cgACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGgttgt                                                                                                             >  1:407697/1‑150 (MQ=255)
                                       cgACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGgttgt                                                                                                             >  1:321743/1‑150 (MQ=255)
                                              tttCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCgg                                                                                                      >  1:1243684/1‑150 (MQ=255)
                                              tttCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCgg                                                                                                      >  1:605619/1‑150 (MQ=255)
                                                 cAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTc                                                                                                   <  2:1411784/150‑1 (MQ=255)
                                                  aaGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCt                                                                                                  >  1:227527/1‑150 (MQ=255)
                                                  aaGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCt                                                                                                  >  2:833959/1‑150 (MQ=255)
                                                   aGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTg                                                                                                 >  1:1466171/1‑150 (MQ=255)
                                                   aGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTg                                                                                                 >  2:848611/1‑150 (MQ=255)
                                                      cTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCt                                                                                              >  2:298262/1‑150 (MQ=255)
                                                       tGGATAGTGCGTAAGGCACCAGAAAGTCACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCtt                                                                                             <  1:396712/150‑1 (MQ=255)
                                                           tAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTc                                                                                         >  2:961797/1‑150 (MQ=255)
                                                             gTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCaa                                                                                       >  2:416553/1‑150 (MQ=255)
                                                              tGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAACTCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAg                                                                                      <  2:125458/150‑1 (MQ=255)
                                                              tGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAg                                                                                      <  2:574164/150‑1 (MQ=255)
                                                              tGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAg                                                                                      >  2:233632/1‑150 (MQ=255)
                                                                 gTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCtt                                                                                   >  2:532340/1‑150 (MQ=255)
                                                                  tAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCAGCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTc                                                                                  <  1:1304315/150‑1 (MQ=255)
                                                                   aaGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTTTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCg                                                                                 <  2:1659147/150‑1 (MQ=255)
                                                                   aaGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCg                                                                                 <  2:978072/150‑1 (MQ=255)
                                                                       cACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCGGGTAAAAAATCAGGCCCACTTTCTGGGGCCGGCTGGAGATTACTTGCCGGGAGCGGGTTGTTCCGCGGGGCTGGCTTTTTCAAGCTTCTCCAg                                                                             >  2:323205/1‑150 (MQ=255)
                                                                       cACCAGAAAGACACAACTTTAACTTTTGTATCATCTGATTTACGGCATCTTGGCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAg                                                                             <  1:1119980/150‑1 (MQ=255)
                                                                         ccAGAAAGACACAACTTTACATTTTGTAGCATCTGAATTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGAt                                                                           >  1:1651857/1‑150 (MQ=255)
                                                                          cAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGCCGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATc                                                                          <  1:1583641/150‑1 (MQ=255)
                                                                           aGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCg                                                                         >  1:1325255/1‑150 (MQ=255)
                                                                            gAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGa                                                                        >  2:901479/1‑150 (MQ=255)
                                                                                   acaACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGat                                                                 <  1:132827/150‑1 (MQ=255)
                                                                                    caACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGata                                                                >  2:1405921/1‑150 (MQ=255)
                                                                                       cTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCAAGATCGAGTGCAatcggt                                                             >  2:1475353/1‑146 (MQ=255)
                                                                                        tttACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCa                                                            >  2:783743/1‑150 (MQ=255)
                                                                                          tACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCAcc                                                          <  1:935238/150‑1 (MQ=255)
                                                                                          tACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCAcc                                                          <  1:1215598/150‑1 (MQ=255)
                                                                                          tACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCAcc                                                          <  1:456225/150‑1 (MQ=255)
                                                                                           aCATTTTGTAGCATCTGATTTACGTCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCg                                                         <  1:1209197/150‑1 (MQ=255)
                                                                                             aTTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGtc                                                       >  2:1038706/1‑150 (MQ=255)
                                                                                              ttttGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGtct                                                      <  1:954260/150‑1 (MQ=255)
                                                                                                    aGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACTGGAGCGGGTTGTTCCGAGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACATTCTCATCCa                                                >  1:353018/1‑150 (MQ=255)
                                                                                                     gCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAg                                               >  2:1114619/1‑150 (MQ=255)
                                                                                                         cTGCTTTACGGCATCTTGTCGCTGTGAAAAAATCAGGCACAATTGCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGGTGTTCCGCTGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATaa                                           <  1:83116/150‑1 (MQ=255)
                                                                                                          tGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTTCGATATTCACCGTCTCATCCAGATAAg                                          >  2:120190/1‑150 (MQ=255)
                                                                                                          tGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAg                                          <  2:1637143/150‑1 (MQ=255)
                                                                                                          tGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAg                                          >  1:1234669/1‑150 (MQ=255)
                                                                                                           gATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAgg                                         >  1:122217/1‑150 (MQ=255)
                                                                                                                 cGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCgg                                   >  2:1569180/1‑150 (MQ=255)
                                                                                                                   gCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCt                                 >  1:27082/1‑150 (MQ=255)
                                                                                                                   gCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCt                                 >  1:993040/1‑150 (MQ=255)
                                                                                                                      tCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCt                              >  1:675086/1‑150 (MQ=255)
                                                                                                                        ttGTGGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTgg                            <  1:350973/150‑1 (MQ=255)
                                                                                                                         ggTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGt                           <  2:1346898/149‑1 (MQ=255)
                                                                                                                             gCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATc                       >  1:704462/1‑150 (MQ=255)
                                                                                                                                gTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCttt                    >  1:75056/1‑150 (MQ=255)
                                                                                                                                 ttAAAAAATCAGGGACATTTTCTTGTGCGTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCATTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTc                   <  2:878354/150‑1 (MQ=255)
                                                                                                                                  tAAATAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGGTGTTCCGCGGGGCTGGCTTTTTGAAGCTTCGCCAGATCGAGTGCGATATTCTCCGTCTCATCCAGATATGGATCCGGCTCCTGGTAATCTTTCg                  <  1:142011/150‑1 (MQ=255)
                                                                                                                                  tAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCg                  >  1:1459088/1‑150 (MQ=255)
                                                                                                                                   aaaaaaTCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCgg                 <  1:918375/150‑1 (MQ=255)
                                                                                                                                    aaaaaTCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGt                >  1:86846/1‑150 (MQ=255)
                                                                                                                                     aaaaTCAGGCACAATTTCTTGTGCCTGATTGATATTTCTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTa               >  1:591857/1‑150 (MQ=255)
                                                                                                                                     aaaaTCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTa               >  2:866826/1‑150 (MQ=255)
                                                                                                                                       aaTCAGTCACAAGAAATTGTGCCAGATTGATATAACTTGACGGGAGCGGTGTGTTCCGAGGGTCTGGCTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCCCCGTCTCATCCATATAAGGATCCGGCTCCTAGTAATCTTTCGGTAGaa            >  2:1589128/1‑149 (MQ=255)
                                                                                                                                       aaTCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGa             >  1:1541967/1‑150 (MQ=255)
                                                                                                                                        aTCAGGCACAAGAAATTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGat            >  1:1010535/1‑150 (MQ=255)
                                                                                                                                        aTCAGGCACAAGAAATTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGat            >  2:1491572/1‑150 (MQ=255)
                                                                                                                                         tCAGGCACAAGAAATTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATACGGCTCCTGGTAATCTTTCGGTAGatc           >  1:773276/1‑150 (MQ=255)
                                                                                                                                          cAGGCAGAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTGGCTCCGCGGGTCTGGCTTTTTCAAGCTGCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTTGTAATCTTTCGGTAGatca          <  2:114893/150‑1 (MQ=255)
                                                                                                                                          cAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGatca          >  1:838248/1‑150 (MQ=255)
                                                                                                                                          cAGGCACAATTTCTTGTGACTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGGCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGatca          <  1:1727981/150‑1 (MQ=255)
                                                                                                                                          cAGGCACAAGAAATTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATGCGGCTCCTGGTAATCTTTCGGTAGatca          >  1:1673560/1‑150 (MQ=255)
                                                                                                                                          cAGGCACAAGAAATTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGatca          >  2:42651/1‑150 (MQ=255)
                                                                                                                                          cAGGCACAAGAAATTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTAAGCGGGTCTGGCTTTTTCACGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGATCCTGGTAATCTTTCGGTAGataa          >  2:1577111/1‑148 (MQ=255)
                                                                                                                                          cAGGAACAATTTCTTGTGCCTGATTGATATTACTTGAGGGGTGGGGGTTGTTCCGCGGGTCTGGCTGTTTCAAGCTTCGGCAGTTCGAGTGCGATATTCACTGTCTCATCCAGTTAAGGATCCGGCTCCTTGTAATCTTTCGGTAGatca          <  2:114894/150‑1 (MQ=255)
                                                                                                                                           aGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGatcat         >  2:1536905/1‑150 (MQ=255)
                                                                                                                                           aGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGatcat         >  2:128138/1‑150 (MQ=255)
                                                                                                                                            ggCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGTTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGatcatc        >  1:533100/1‑150 (MQ=255)
                                                                                                                                             gCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATcc       >  2:1705641/1‑150 (MQ=255)
                                                                                                                                              cacaATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCa      >  1:1844/1‑150 (MQ=255)
                                                                                                                                                caATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGt    >  2:558741/1‑150 (MQ=255)
                                                                                                                                                 aaTTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGtt   <  2:1444025/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAAAACGCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTT  >  NC_000913/1912601‑1912896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: