Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,926,891 G→C 6.4% S650* (TCA→TGA)  ptrB ← protease II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,926,8910GC 6.4% 320.0 / 7.6 99S650* (TCA→TGA) ptrBprotease II
Reads supporting (aligned to +/- strand):  ref base G (46/46);  new base C (3/3);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

TGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCGGTGACG  >  NC_000913/1926745‑1927040
                                                                                                                                                  |                                                                                                                                                     
tGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGt                                                                                                                                                    >  1:1031685/1‑150 (MQ=255)
tGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGt                                                                                                                                                    >  2:1472206/1‑150 (MQ=255)
tGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTACGCAGGCGTAGCGGGTAATGTTCCCTGCGCAAGCGCGA‑AATAAAAGCATATTCAATCGCTACGCCTTCGTACTATTTAAAGCGACCAGATTTACATCCATGGCCTAAGTc                                                                                                                                                   >  1:1469344/1‑150 (MQ=255)
  cGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTcc                                                                                                                                                  <  2:674972/150‑1 (MQ=255)
   gTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCa                                                                                                                                                 >  1:1100622/1‑150 (MQ=255)
   gTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCa                                                                                                                                                 >  2:672318/1‑150 (MQ=255)
    tttAACACTGAACCATTATCTGGAAAATACTTATTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAACGCATATTCCATCGCTACGCCTTCGTACGATTTAACGCGACCAGATTTACCGCCATGGCCTGAGTCCAt                                                                                                                                                <  2:119587/150‑1 (MQ=255)
    tttAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCAt                                                                                                                                                >  2:1329267/1‑150 (MQ=255)
      tAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGt                                                                                                                                              >  1:550511/1‑150 (MQ=255)
         cacTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCgg                                                                                                                                           <  1:769722/150‑1 (MQ=255)
         cacTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCgg                                                                                                                                           >  1:1505154/1‑150 (MQ=255)
           cTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTa                                                                                                                                         >  1:1022810/1‑150 (MQ=255)
           cTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTa                                                                                                                                         >  1:1486721/1‑150 (MQ=255)
           cTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGTa                                                                                                                                         <  1:727951/150‑1 (MQ=255)
           cTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGAGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTa                                                                                                                                         >  1:1614561/1‑150 (MQ=255)
            tGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTAc                                                                                                                                        <  2:1556256/150‑1 (MQ=255)
             gAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACa                                                                                                                                       >  1:338518/1‑150 (MQ=255)
                aCATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGTaaatct                                                                                                                                    <  2:144015/150‑6 (MQ=255)
                aCATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGTaaatct                                                                                                                                    <  2:1583257/150‑6 (MQ=255)
                 cATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGc                                                                                                                                   >  2:1101633/1‑150 (MQ=255)
                    tATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAAt                                                                                                                                <  1:1376094/150‑1 (MQ=255)
                    tATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAACATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAAt                                                                                                                                >  2:550615/1‑150 (MQ=255)
                      tCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGCTTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATaa                                                                                                                              >  1:172393/1‑150 (MQ=255)
                        tGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATaaaa                                                                                                                            <  2:1290769/150‑1 (MQ=255)
                         ggAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAg                                                                                                                           <  1:663643/150‑1 (MQ=255)
                          gAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGa                                                                                                                          >  2:443750/1‑150 (MQ=255)
                            aaaTACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATg                                                                                                                        >  1:819101/1‑150 (MQ=255)
                            aaaTACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATg                                                                                                                        <  1:1639604/150‑1 (MQ=255)
                                 cTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCAt                                                                                                                   >  2:912286/1‑150 (MQ=255)
                                    aGTCCGCAGGCGGAGCGGGTAATGTTCACTGCGCCCGCGCGCCCAGAAAAGCATATTCCATAGCAACCCCTGCGTACGATTTAAAGCGACCAGATTTACCGCCAAAGCCGGAGGCCATGTAGGTTCAGAGCAAGAAAACATGTTCATCgg                                                                                                                >  2:1656725/1‑150 (MQ=255)
                                         gCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTc                                                                                                           <  2:1396287/150‑1 (MQ=255)
                                          cAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATGGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCa                                                                                                          <  1:239514/150‑1 (MQ=255)
                                          cAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCa                                                                                                          >  2:1677272/1‑150 (MQ=255)
                                          cAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCATTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCa                                                                                                          <  1:1440400/150‑1 (MQ=255)
                                            ggCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGACATAGGTTTTCAGc                                                                                                        >  2:262355/1‑150 (MQ=255)
                                                 aGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTcgcg                                                                                                   <  1:1333538/150‑1 (MQ=255)
                                                 aGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTcgcg                                                                                                   <  2:771792/150‑1 (MQ=255)
                                                 aGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTcgcg                                                                                                   <  2:874156/150‑1 (MQ=255)
                                                    gggTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCaa                                                                                                >  2:1344794/1‑150 (MQ=255)
                                                      gTAATGCTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAAtt                                                                                              <  2:209372/150‑1 (MQ=255)
                                                       tAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAAttt                                                                                             <  2:1234211/150‑1 (MQ=255)
                                                         aTGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCCTGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAg                                                                                           <  2:301109/150‑1 (MQ=255)
                                                          tGTTGCCTTCGCCACCGGGAACAGAAAAGCATATTCCCTCGCTACGCCGTCGTTCGATTTAAAGCGACCACATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGc                                                                                          <  1:472504/150‑1 (MQ=255)
                                                           gTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCg                                                                                         >  2:97532/1‑150 (MQ=255)
                                                             tCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGAc                                                                                       >  2:1511688/1‑150 (MQ=255)
                                                               ccTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATATAAGATGGTCTTCGGTTTTCAGCTCGCGCAATTTAGCGAccc                                                                                     >  2:265284/1‑150 (MQ=255)
                                                               ccTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGAccc                                                                                     >  2:88580/1‑150 (MQ=255)
                                                                cTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCTAAATTGCGCGagctg                                                                                    <  2:1100622/150‑5 (MQ=255)
                                                                    gcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCAtttt                                                                                <  1:488136/150‑1 (MQ=255)
                                                                     ccAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTg                                                                               >  1:616360/1‑150 (MQ=255)
                                                                       aGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGcc                                                                             <  2:1229903/150‑1 (MQ=255)
                                                                          gcgACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGt                                                                          <  1:1388533/150‑1 (MQ=255)
                                                                          gcgACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGt                                                                          <  2:910807/150‑1 (MQ=255)
                                                                              ccAGAAAAGCATATTCCATCGCTACGCCTTAGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTccc                                                                      >  1:292732/1‑150 (MQ=255)
                                                                                   aaaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAatat                                                                 <  1:1197166/150‑1 (MQ=255)
                                                                                    aatcATATTCCATCGCTACGCCTTCGTAAGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATAtt                                                                <  1:1373880/147‑1 (MQ=255)
                                                                                     aacATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTg                                                               <  1:550615/148‑1 (MQ=255)
                                                                                        atatTGAATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCAc                                                            <  1:1109091/150‑1 (MQ=255)
                                                                                          atTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCt                                                          <  1:912286/150‑1 (MQ=255)
                                                                                          atTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCt                                                          >  1:648746/1‑150 (MQ=255)
                                                                                           ttCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTg                                                         <  1:1029304/150‑1 (MQ=255)
                                                                                              cATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTgaga                                                      >  2:632735/1‑150 (MQ=255)
                                                                                                  gCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCg                                                  >  2:769028/1‑150 (MQ=255)
                                                                                                   cTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGt                                                 >  2:53890/1‑150 (MQ=255)
                                                                                                      cGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCAGTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCa                                              >  2:1536372/1‑150 (MQ=255)
                                                                                                         cTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAAc                                           <  1:672318/150‑1 (MQ=255)
                                                                                                          ttCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCACAATATTGCACCTGAGAATCGTGCAAAcc                                          <  2:304908/150‑1 (MQ=255)
                                                                                                            cGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCgg                                        >  1:1257203/1‑150 (MQ=255)
                                                                                                              tACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTc                                      <  1:1101633/150‑1 (MQ=255)
                                                                                                               aCGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCg                                     >  2:725763/1‑150 (MQ=255)
                                                                                                                 gATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGtt                                   >  1:928792/1‑150 (MQ=255)
                                                                                                                 gATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGtt                                   >  1:455180/1‑150 (MQ=255)
                                                                                                                    ttAAAGCGACCAGATTTACCGCCTTGGCCTGAGTCCATGTCGGTAGAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTAcc                                <  2:1395017/150‑1 (MQ=255)
                                                                                                                    ttAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTAcc                                <  1:787292/150‑1 (MQ=255)
                                                                                                                    ttAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTAcc                                <  2:1150089/150‑1 (MQ=255)
                                                                                                                     tAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGATCGCGCAATTTCGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGGGCAACCCGGGCGTTAccc                               >  1:340713/1‑149 (MQ=255)
                                                                                                                      aaaGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAg                              <  2:118463/150‑1 (MQ=255)
                                                                                                                      aaaGAGACCTGATTTACCGCCATGGCCGGAGTCCATGTCGGTACAGAGCAATACAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCTACCCATTTTGCCGTTTCCCAATATTTCACATGAGAATCGTGCAAACCGGTCGTTAACAg                              <  2:292615/150‑1 (MQ=255)
                                                                                                                       aaGCGACCAGAATTACCGCCATGGCCTGAGTCCATGTCTGTACAGAGCAATAAAAGATGGGCATCGGTTTTCAGCTCTCGCAATTTAGCGACCCATTTTGCCTGATCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGt                             <  1:559336/150‑1 (MQ=255)
                                                                                                                        aGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTa                            <  1:1048427/150‑1 (MQ=255)
                                                                                                                        aGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTa                            <  1:1087498/150‑1 (MQ=255)
                                                                                                                            aCCAGTTTTACCGCCATTGCCTGAGTCCATGTCGGTACAGAGAAATAAAAGATGGTCTTCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTTAGAATCGTGCAAACAGGTCGTTACAAGTAAATg                        <  2:72634/150‑1 (MQ=255)
                                                                                                                            aCCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAATCCTGTCGTTACCAGTAAAtt                        >  1:395938/1‑149 (MQ=255)
                                                                                                                            aCCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATg                        >  2:1679909/1‑150 (MQ=255)
                                                                                                                             ccAGATTTACCGCCATGGCCTGCGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACGGGTCGTTACCAGTAAATGc                       >  1:863969/1‑150 (MQ=255)
                                                                                                                               aGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTTAATGCgg                     >  2:1517086/1‑150 (MQ=255)
                                                                                                                                gATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGa                    >  2:1387726/1‑150 (MQ=255)
                                                                                                                                  tttACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTAAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATa                  <  2:338518/150‑1 (MQ=255)
                                                                                                                                     aCCGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGc               >  2:675376/1‑150 (MQ=255)
                                                                                                                                     aCCGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGc               >  1:824566/1‑150 (MQ=255)
                                                                                                                                      ccGCCATGGCCTGGGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGcc              <  1:434918/150‑1 (MQ=255)
                                                                                                                                      ccGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCATCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGAACATGAGACTCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGcc              >  2:174743/1‑150 (MQ=255)
                                                                                                                                        gCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCtg            >  1:614077/1‑150 (MQ=255)
                                                                                                                                           ctGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTGTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGGCGTTACCAGTAAATGCGGATAAGCCTGGGc         <  2:1411804/149‑1 (MQ=255)
                                                                                                                                             ggCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCgg       >  1:730011/1‑150 (MQ=255)
                                                                                                                                              gCCGGAGTCCATGTCGGTTCAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCGGt      <  2:1077634/150‑1 (MQ=255)
                                                                                                                                               ccTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCGGTg     >  2:840995/1‑150 (MQ=255)
                                                                                                                                               ccTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCGGTg     <  1:705273/150‑1 (MQ=255)
                                                                                                                                               ccTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTATCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCGGTg     >  1:980600/1‑150 (MQ=255)
                                                                                                                                                  gAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCGGTGACg  <  2:1614561/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGTCGTTACCAGTAAATGCGGATAAGCCTGTGCGGTGACG  >  NC_000913/1926745‑1927040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑